Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,108,760 A→C H132Q (CAT→CAG)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,108,7600AC89.7% 451.2 / 21.8 156H132Q (CAT→CAG) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (5/11);  new base C (57/83);  total (62/94)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

ATGAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGG  >  NC_000913/3108664‑3108855
                                                                                                |                                                                                               
aTGAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGc                                                                                              <  1:2749608/100‑1 (MQ=255)
aTGAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGc                                                                                              <  1:2749137/100‑1 (MQ=255)
 tGAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGgctgct                                                                                             <  1:608211/100‑1 (MQ=255)
  gAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCtt                                                                                            <  1:1795902/100‑1 (MQ=255)
   aaTAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGGGAAAGACGTTGTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCttt                                                                                           >  2:3996883/1‑100 (MQ=255)
   aaTAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCttt                                                                                           >  1:2610983/1‑100 (MQ=255)
   aaTAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCttt                                                                                           <  1:2927440/100‑1 (MQ=255)
   aaTAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCttt                                                                                           <  2:1673470/100‑1 (MQ=255)
    aTAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCtttt                                                                                          >  2:3083547/1‑100 (MQ=255)
     tAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCttttt                                                                                         <  1:462367/100‑1 (MQ=255)
      aaGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTa                                                                                        >  2:1717916/1‑100 (MQ=255)
       aGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTaa                                                                                       <  1:1591584/100‑1 (MQ=255)
       aGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTaa                                                                                       <  2:2032032/100‑1 (MQ=255)
        gCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTaaa                                                                                      <  2:2490684/100‑1 (MQ=255)
         cAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTaaaa                                                                                     >  2:1262708/1‑100 (MQ=255)
          aGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTaaaaa                                                                                    >  1:2718894/1‑100 (MQ=255)
           gATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTaaaaaa                                                                                   >  2:2045508/1‑100 (MQ=255)
            gtCGCCCAATTTTACGTCAGCGTTACACATATCGGAGCGAAAGACGCTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAAc                                                                                  <  2:2965132/99‑1 (MQ=255)
               gCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCa                                                                               >  1:1026964/1‑100 (MQ=255)
               gCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCa                                                                               >  1:1921713/1‑100 (MQ=255)
                 ccAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCAcc                                                                             <  2:1399169/100‑1 (MQ=255)
                 ccAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCAcc                                                                             >  2:841532/1‑100 (MQ=255)
                   aaTTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCAt                                                                           <  2:2981250/100‑1 (MQ=255)
                    aTTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCAccttt                                                                          <  1:117362/100‑4 (MQ=255)
                     ttttACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGt                                                                         <  2:2877831/100‑1 (MQ=255)
                         aCGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGAt                                                                     <  1:726887/100‑1 (MQ=255)
                          cGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATg                                                                    >  2:2467452/1‑100 (MQ=255)
                           gTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGt                                                                   >  2:3965045/1‑100 (MQ=255)
                            tCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTc                                                                  >  1:1144356/1‑100 (MQ=255)
                            tCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTc                                                                  >  1:1101802/1‑100 (MQ=255)
                             cAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTcc                                                                 <  1:1689781/100‑1 (MQ=255)
                             cAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTcc                                                                 >  2:1396762/1‑100 (MQ=255)
                               gCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAg                                                               <  1:2605558/100‑1 (MQ=255)
                               gCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAg                                                               <  2:2170978/100‑1 (MQ=255)
                               gCGTGAAACATACCGGCGCGAAAGACGTTCTCACAAAAGAAATCGTAAAACTGTCGTCCGGCAAAAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAg                                                               <  2:3439857/100‑1 (MQ=255)
                                  ttACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgc                                                            <  1:2460834/100‑1 (MQ=255)
                                  ttACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgc                                                            >  2:2145436/1‑100 (MQ=255)
                                  ttACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgc                                                            <  1:859727/100‑1 (MQ=255)
                                  gtACACATATCGGCGCGAAAGACGTGCTCGCCAAAGAAATCGGTAAACGGGCGTCCGGCAGGCTGCTTGTTAAAAAACGCGCCATGTTGATGTCCAGGgc                                                            <  2:2253572/99‑1 (MQ=255)
                                   tACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgca                                                           <  2:1079685/100‑1 (MQ=255)
                                   ctcacaTATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgca                                                           <  2:263689/98‑1 (MQ=255)
                                     cacaTATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgcaag                                                         >  1:1033269/1‑100 (MQ=255)
                                     cacaTATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgcaag                                                         >  1:268098/1‑100 (MQ=255)
                                     cacaTATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgcaag                                                         >  1:2707803/1‑100 (MQ=255)
                                     aacaTATCGGCGCGAAAGACGTTCTCACCAAAGAACTAGTAAAAATGGCGTCCAGAAGAAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAGGgcaag                                                         <  2:870577/99‑1 (MQ=255)
                                       caTATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGgcaagca                                                       >  1:719325/1‑100 (MQ=255)
                                         tatCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCaaa                                                     >  1:2323974/1‑100 (MQ=255)
                                          atCTGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAg                                                    <  2:2829792/100‑1 (MQ=255)
                                            cGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGt                                                  >  1:3168702/1‑100 (MQ=255)
                                             ggCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTg                                                 >  2:197792/1‑100 (MQ=255)
                                              gcgcGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGc                                                <  1:36100/100‑1 (MQ=255)
                                              gcgcGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGc                                                >  1:1647232/1‑100 (MQ=255)
                                                 cgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGt                                             >  2:1301668/1‑100 (MQ=255)
                                                 cgAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCACGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGt                                             <  2:3746132/100‑1 (MQ=255)
                                                    aaGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGtaga                                          >  1:2791121/1‑97 (MQ=255)
                                                    aaGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGc                                          <  2:3537614/100‑1 (MQ=255)
                                                    aaGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGc                                          >  2:521873/1‑100 (MQ=255)
                                                     aGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGcc                                         <  1:1765268/100‑1 (MQ=255)
                                                     aGACGATCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGAAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGcc                                         <  2:245679/100‑1 (MQ=255)
                                                      gACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCATCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGccc                                        <  1:1383343/100‑1 (MQ=255)
                                                      gACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGccc                                        <  1:2486495/100‑1 (MQ=255)
                                                          ttCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCAtct                                    >  1:872179/1‑100 (MQ=255)
                                                            ctcACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCa                                  <  1:518617/100‑1 (MQ=255)
                                                            ctcACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCA‑CATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCaa                                 >  1:3824386/1‑100 (MQ=255)
                                                              cACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAAc                                <  1:739711/100‑1 (MQ=255)
                                                               aCCAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACg                               >  1:287166/1‑100 (MQ=255)
                                                               aCCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACg                               >  2:3373549/1‑100 (MQ=255)
                                                                ccAAAGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGt                              >  1:1088977/1‑100 (MQ=255)
                                                                ccAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGTTGTTGCCCATCGCAACGt                              <  1:3397835/100‑1 (MQ=255)
                                                                  aaaGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTAc                            >  1:585852/1‑100 (MQ=255)
                                                                  aaaGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTAc                            <  1:1311013/100‑1 (MQ=255)
                                                                  aaaGAAATCGTAAAAATGGCGTCCGGCAGGAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTAc                            <  2:3478337/100‑1 (MQ=255)
                                                                  aaaGAAATCGTAAAAATGGCGTCCGGCAGGAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTAc                            <  2:502808/100‑1 (MQ=255)
                                                                   aaGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACt                           <  1:4027758/100‑1 (MQ=255)
                                                                   aaGAAATCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACt                           <  2:759744/100‑1 (MQ=255)
                                                                   aaGAAATCGTAAAAATGGCGTCCGGCAGAAAAAAAAAAAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACt                           <  2:1015570/100‑1 (MQ=255)
                                                                      aaaTCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCg                        <  1:2242341/100‑1 (MQ=255)
                                                                        aTCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTc                      <  1:384048/100‑1 (MQ=255)
                                                                         tCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCa                     <  1:1883403/100‑1 (MQ=255)
                                                                         tCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCa                     <  1:1472184/100‑1 (MQ=255)
                                                                         tCGTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCa                     <  2:2279953/100‑1 (MQ=255)
                                                                           gTAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGc                   >  1:2388321/1‑100 (MQ=255)
                                                                           gTAAAAATGACGTCCGGCAGGCTGCCTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTAAGc                   >  2:976485/1‑100 (MQ=255)
                                                                            tAAAAATGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCg                  >  2:658037/1‑100 (MQ=255)
                                                                               aaaTGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCgtgt               >  2:1515084/1‑100 (MQ=255)
                                                                                aaTGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTc              >  1:1861624/1‑100 (MQ=255)
                                                                                 aTGTCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCa             <  2:1880683/100‑1 (MQ=255)
                                                                                 aTGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCa             >  1:3045329/1‑100 (MQ=255)
                                                                                  tGGCGTCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTACGTCAGCGTGTCAt            <  1:2910995/100‑1 (MQ=255)
                                                                                       tCCGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAAc       >  2:3878710/1‑100 (MQ=255)
                                                                                       accGGCAGGATGCTGTGTAAAAAACGCACCAGGTTGATGTCCAGGGCAAGCAAGGGTGCTGTTGTCCATCTCAGCGTACTGCGTCAGCGTGTCATaaagc       <  2:2594098/99‑3 (MQ=255)
                                                                                        ccGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACg      <  1:2021008/100‑1 (MQ=255)
                                                                                         cGGCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACgg     <  2:2793283/100‑1 (MQ=255)
                                                                                          ggCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGt    <  1:3073643/100‑1 (MQ=255)
                                                                                          ggCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGt    <  1:3159217/100‑1 (MQ=255)
                                                                                          ggCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGt    >  1:1421288/1‑100 (MQ=255)
                                                                                           gCAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCTATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTg   <  2:3913769/100‑1 (MQ=255)
                                                                                           gCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTg   <  1:3441589/100‑1 (MQ=255)
                                                                                            cAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGTTgg  <  1:3136592/100‑1 (MQ=255)
                                                                                            cAGGCTGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTgg  <  1:1540565/100‑1 (MQ=255)
                                                                                                |                                                                                               
ATGAATAAGCAGATCGCCCAATTTTACGTCAGCGTTACACATATCGGCGCGAAAGACGTTCTCACCAAAGAAATCGTAAAAATGGCGTCCGGCAGGATGCTTTTTAAAAAACGCACCATGTTGATGTCCAGGGCAAGCAAAGGTGCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGG  >  NC_000913/3108664‑3108855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑