BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,612	A→C	L262R (CTG→CGG)	parE ←	DNA topoisomerase IV, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,612	0	A	C	100.0%	505.2 / NA	140	L262R (CTG→CGG)	parE	DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (76/64); total (76/64)

Only 100 of 143 total aligned reads displayed.

ACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGC  >  NC_000913/3174517‑3174707

aCTCACGCATCGCGTCCAACAGGCCTTGACGCAGACCCTTAACATGCGTACCGCCCTGCATCGTTTGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTc                                                                                             <  1:3793019/100‑1 (MQ=255)

aCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTc                                                                                             <  1:1596434/100‑1 (MQ=255)

 cTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCa                                                                                            >  1:1290760/1‑100 (MQ=255)

  tCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAc                                                                                           <  1:898642/100‑1 (MQ=255)

   cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                          >  1:3296409/1‑100 (MQ=255)

   cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                          >  2:356682/1‑100 (MQ=255)

   cACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCAGCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCAcc                                                                                          <  2:3594050/100‑1 (MQ=255)

    aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCg                                                                                         <  1:1061176/100‑1 (MQ=255)

    aCGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCg                                                                                         <  2:3274220/100‑1 (MQ=255)

        aTCGCGTCCAACAGGCCCTGACGCAGACCTTTAACATGGGTACCGCCCTGCATCGTTGTGAGAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCtt                                                                                     >  1:3906152/1‑100 (MQ=255)

        aTCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCtt                                                                                     >  2:2422374/1‑100 (MQ=255)

          cgcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTcc                                                                                   <  1:749577/100‑1 (MQ=255)

           gcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCg                                                                                  <  1:1480130/100‑1 (MQ=255)

           gcgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCg                                                                                  >  1:2606481/1‑100 (MQ=255)

            cgTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCgg                                                                                 <  1:3109086/100‑1 (MQ=255)

            cgTCCAACACGCCCTGACGCAGATCATTAACATGGGTACCGCTCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCgg                                                                                 <  1:3560003/100‑1 (MQ=255)

             gTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGc                                                                                <  1:925860/100‑1 (MQ=255)

              tCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCa                                                                               <  2:2791836/100‑1 (MQ=255)

               ccAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCCGCCGTTTACCGCCTTCCGGCAg                                                                              >  2:3364858/1‑100 (MQ=255)

               ccAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAg                                                                              <  2:1830995/100‑1 (MQ=255)

                cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                             >  2:2054024/1‑100 (MQ=255)

                cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                             >  1:3704887/1‑100 (MQ=255)

                cAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGc                                                                             >  1:2281437/1‑100 (MQ=255)

                 aaCAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGcc                                                                            <  2:919663/100‑1 (MQ=255)

                   cAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcac                                                                          <  1:1932028/100‑1 (MQ=255)

                   cAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcac                                                                          >  1:2016069/1‑100 (MQ=255)

                   cAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcac                                                                          <  1:3445953/100‑1 (MQ=255)

                    aGGACCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCcaca                                                                         <  2:3816694/100‑1 (MQ=255)

                     ggCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTTGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAg                                                                        >  2:1355490/1‑100 (MQ=255)

                     ggCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAg                                                                        >  1:1348410/1‑100 (MQ=255)

                      gCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGt                                                                       <  2:1324540/100‑1 (MQ=255)

                      gCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATAGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGt                                                                       <  2:1821569/100‑1 (MQ=255)

                             cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                >  2:417827/1‑100 (MQ=255)

                             cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                <  2:2067639/100‑1 (MQ=255)

                             cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                <  1:2366724/100‑1 (MQ=255)

                             cGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                <  1:417968/100‑1 (MQ=255)

                             cGCAGACCATTAACAGGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGccc                                                                <  2:3333202/100‑1 (MQ=255)

                             cGCAGACCAGTAACATGGTTACCGCCCTTCATCGTTGAGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCCCAGTAGCGccc                                                                >  2:971445/1‑100 (MQ=255)

                                  aCCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTcc                                                           <  2:705164/100‑1 (MQ=255)

                                  aCCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTcc                                                           <  1:1052290/100‑1 (MQ=255)

                                  aCCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTcc                                                           >  1:4007323/1‑100 (MQ=255)

                                   ccATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                          >  1:579508/1‑100 (MQ=255)

                                   ccATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCca                                                          >  2:1015078/1‑100 (MQ=255)

                                    cATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTAGACGTAGCTTTCCGTCAGCCGATCACCGCCTTCCGGCAGCCACTGTAGCGCCCAGTCcac                                                         >  2:1697209/1‑100 (MQ=255)

                                      ttAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAg                                                       >  1:2928335/1‑100 (MQ=255)

                                      ttAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAg                                                       <  2:1128199/100‑1 (MQ=255)

                                       tAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGc                                                      <  1:1303939/100‑1 (MQ=255)

                                        aaCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCt                                                     >  2:3932998/1‑100 (MQ=255)

                                        aaCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCt                                                     >  1:768536/1‑100 (MQ=255)

                                         aCATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCtt                                                    >  1:945433/1‑100 (MQ=255)

                                           aTGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCa                                                  <  1:3117853/100‑1 (MQ=255)

                                           aTGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCATTCACCGCATTCCGGCAGCCCCAGTAGCGCCCAGTCCACAGCTTCa                                                  >  1:3792479/1‑100 (MQ=255)

                                            tGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAg                                                 <  2:2703083/100‑1 (MQ=255)

                                            tGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAg                                                 >  1:904839/1‑100 (MQ=255)

                                            tGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAg                                                 >  2:2116969/1‑100 (MQ=255)

                                              ggTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTa                                               >  1:1656344/1‑100 (MQ=255)

                                              ggTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTa                                               >  1:2552969/1‑100 (MQ=255)

                                              ggTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTa                                               <  2:1267014/100‑1 (MQ=255)

                                              ggTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTa                                               >  1:2672842/1‑100 (MQ=255)

                                              ggTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTa                                               <  1:829255/100‑1 (MQ=255)

                                               gTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACAGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTAt                                              >  2:1679997/1‑100 (MQ=255)

                                                  ccGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAc                                           >  1:3615541/1‑100 (MQ=255)

                                                  ccGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAc                                           >  1:1770723/1‑100 (MQ=255)

                                                    gCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCa                                         >  1:806660/1‑100 (MQ=255)

                                                    gCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCa                                         >  1:2576031/1‑100 (MQ=255)

                                                     cccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAg                                        <  1:4023163/100‑1 (MQ=255)

                                                      ccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGc                                       >  2:2651262/1‑100 (MQ=255)

                                                      ccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGc                                       <  1:457688/100‑1 (MQ=255)

                                                      ccTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGc                                       <  1:2505789/100‑1 (MQ=255)

                                                       cTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCg                                      >  1:1743005/1‑100 (MQ=255)

                                                         gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGaa                                    >  1:1094428/1‑100 (MQ=255)

                                                         gCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGaa                                    >  1:3172080/1‑100 (MQ=255)

                                                          cATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACGGGTTCAGTATCACCAGCGaaa                                   <  1:3483833/100‑1 (MQ=255)

                                                            tCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAAtt                                 <  1:133111/100‑1 (MQ=255)

                                                                 gggATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGa                            <  2:1995211/100‑1 (MQ=255)

                                                                  ggATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGAt                           >  1:2882773/1‑100 (MQ=255)

                                                                  ggATAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGAt                           >  2:873618/1‑100 (MQ=255)

                                                                   gctAAGGGTGACGTAGCTTTCTGTCAGCCGTTGACCGCCTTGCGGCAGGCACAGTGGCGCCCGGTCCACAGCTTCAGTATCACCCGCGAAACTACCGATa                          >  2:2532285/3‑100 (MQ=255)

                                                                    aTAAGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATaa                         >  2:1034494/1‑100 (MQ=255)

                                                                       aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTATCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACg                      <  1:3645268/100‑1 (MQ=255)

                                                                       aGGTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACg                      <  2:3924400/100‑1 (MQ=255)

                                                                        ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                     >  2:3251129/1‑100 (MQ=255)

                                                                        ggTTGACGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACgg                     >  2:1114721/1‑100 (MQ=255)

                                                                              cGTAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCAACAGCGAAATTAATGATAAACGGttcttc               >  1:630775/1‑100 (MQ=255)

                                                                              cGTAGCTTTCGCTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTc               <  2:2724036/100‑1 (MQ=255)

                                                                                tAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCg             <  2:74018/100‑1 (MQ=255)

                                                                                tAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCg             <  2:996187/100‑1 (MQ=255)

                                                                                tAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCg             >  2:2125678/1‑100 (MQ=255)

                                                                                tAGCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCg             <  1:2854266/100‑1 (MQ=255)

                                                                                  gCTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGc           <  1:75444/100‑1 (MQ=255)

                                                                                   cTTTCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCa          >  2:225481/1‑100 (MQ=255)

                                                                                    tttCGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTCTTCCGGCAg         >  1:3561852/1‑100 (MQ=255)

                                                                                       cGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGt      >  2:2843635/1‑100 (MQ=255)

                                                                                       cGGTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGt      >  1:2470245/1‑100 (MQ=255)

                                                                                        ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTc     <  1:2759439/100‑1 (MQ=255)

                                                                                        ggTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTc     >  1:1660197/1‑100 (MQ=255)

                                                                                         ntCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCg    >  1:3244677/2‑100 (MQ=255)

                                                                                         gTCAGCCGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTCCCGATAAACGGTTTTTCCGGCAGCGTCg    <  2:1086/100‑1 (MQ=255)

                                                                                           cagccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGc  >  2:379844/1‑100 (MQ=255)

                                                                                           cagccgTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGc  >  1:224140/1‑100 (MQ=255)

ACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGGGATAAGGTTGACGTAGCTTTCGGTCAGCAGTTCACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGC  >  NC_000913/3174517‑3174707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`