Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,182,529 A→C intergenic (‑122/‑21) ygiD ← / → zupT 4,5‑ DOPA‑extradiol‑dioxygenase/zinc transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,182,5290AC96.8% 503.8 / ‑1.8 155intergenic (‑122/‑21)ygiD/zupT4,5‑ DOPA‑extradiol‑dioxygenase/zinc transporter
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (83/67);  total (86/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG  >  NC_000913/3182431‑3182624
                                                                                                  |                                                                                               
  aTTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGa                                                                                              <  2:1288690/100‑1 (MQ=255)
   ttttGATATCCTAACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGAGGATCTGACTGAAGGTGCAGAt                                                                                             >  1:3368826/1‑100 (MQ=255)
      tGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTta                                                                                          <  2:1031070/100‑1 (MQ=255)
      tGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAAGTGCAGAttga                                                                                          <  2:3702891/100‑3 (MQ=255)
        atatCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTtata                                                                                        <  2:1352816/100‑1 (MQ=255)
         tatCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATaa                                                                                       <  2:1117883/100‑1 (MQ=255)
          atCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAg                                                                                      <  2:2421227/100‑1 (MQ=255)
          atCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAg                                                                                      <  2:2675647/100‑1 (MQ=255)
          atCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAg                                                                                      <  2:1254827/100‑1 (MQ=255)
           ttcTCACAATACGCTTGTTCGGCGGAGTAAGAACACGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAgg                                                                                     <  2:1774609/98‑1 (MQ=255)
           tCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAgg                                                                                     >  2:641191/1‑100 (MQ=255)
           tCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAgg                                                                                     >  1:888636/1‑100 (MQ=255)
           tCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAgg                                                                                     >  2:2075923/1‑100 (MQ=255)
             cTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAg                                                                                   <  1:823762/100‑1 (MQ=255)
              tCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGt                                                                                  >  1:184903/1‑100 (MQ=255)
              tCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGt                                                                                  >  1:265352/1‑100 (MQ=255)
              tCACAAGACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGt                                                                                  <  2:3932373/100‑1 (MQ=255)
                acaATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTg                                                                                <  2:1239786/100‑1 (MQ=255)
                acaATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTg                                                                                >  1:3271681/1‑100 (MQ=255)
                  aaTACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTc                                                                              <  1:3459626/100‑1 (MQ=255)
                       gCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGt                                                                         >  2:2916571/1‑100 (MQ=255)
                       gCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGt                                                                         >  1:2572201/1‑100 (MQ=255)
                        cTTGTTCGGCGGAGTAAGAACCCGGATCACAATGGTGATGAGCAACAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTc                                                                        <  2:3816043/100‑1 (MQ=255)
                        cTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTc                                                                        >  2:705577/1‑100 (MQ=255)
                        cTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTc                                                                        >  1:2863095/1‑100 (MQ=255)
                        attGTTCGGCGGAGTACGAACCCGGATAACAATGATGATGATAATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTc                                                                        <  2:181862/99‑1 (MQ=255)
                          tGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAg                                                                      >  1:1983270/1‑100 (MQ=255)
                           gTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGt                                                                     >  1:1902781/1‑100 (MQ=255)
                           gTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGt                                                                     >  2:739913/1‑100 (MQ=255)
                           gTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGt                                                                     >  1:1035446/1‑100 (MQ=255)
                              cggcggAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTAcc                                                                  >  1:2639326/1‑100 (MQ=255)
                               ggcggAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACct                                                                 <  1:2515908/100‑1 (MQ=255)
                                gcggAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACctc                                                                <  2:2658248/100‑1 (MQ=255)
                                gcggAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACctc                                                                <  2:335437/100‑1 (MQ=255)
                                    aGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCAt                                                            >  1:1441976/1‑100 (MQ=255)
                                     gTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCAtt                                                           >  1:3620188/1‑100 (MQ=255)
                                     gTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCAtt                                                           >  1:1954487/1‑100 (MQ=255)
                                     gTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCAtt                                                           >  2:2595392/1‑100 (MQ=255)
                                     gTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCAtt                                                           >  1:702017/1‑100 (MQ=255)
                                      tAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTc                                                          <  1:1075778/100‑1 (MQ=255)
                                      tAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTc                                                          <  1:408605/100‑1 (MQ=255)
                                       aaGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCt                                                         >  1:2297969/1‑100 (MQ=255)
                                       aaGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCt                                                         >  1:1801372/1‑100 (MQ=255)
                                       aaGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCt                                                         >  2:2729967/1‑100 (MQ=255)
                                        aGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTg                                                        >  1:1567144/1‑100 (MQ=255)
                                        aGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTg                                                        <  1:731754/100‑1 (MQ=255)
                                        aGAACCCGGATAACAATGATGATGAGCATCAGCTATATTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCCCATTCTg                                                        >  2:1026882/1‑100 (MQ=255)
                                          aaCCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTgcc                                                      >  2:2990664/1‑98 (MQ=255)
                                          aaCCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGAc                                                      <  1:1429874/100‑1 (MQ=255)
                                           ncccGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGAcc                                                     >  1:206585/2‑100 (MQ=255)
                                           aCCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGTCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGAcc                                                     <  2:3761483/100‑1 (MQ=255)
                                           aCCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGAcc                                                     >  2:2502302/1‑100 (MQ=255)
                                            cccGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCa                                                    >  1:967099/1‑100 (MQ=255)
                                             ccGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCAt                                                   >  1:2299184/1‑100 (MQ=255)
                                               ggATAACAATGATGATGATCGCCATTTATTTTGTCTTTGTGCCTGAAGGTGCAGATGTATAAGGAGTTGTCTATGTCAGTACCTTTCATGCTGACCATAc                                                 <  2:82841/100‑1 (MQ=255)
                                               ggATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCATATTTATAAGGAGTTGTCGATGTCAGTACCTCTCTTTCTTACCATAc                                                 <  2:1777368/100‑1 (MQ=255)
                                               ggATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATAc                                                 >  1:1707561/1‑100 (MQ=255)
                                               gcaTAACAATGATGATGATCATCAGTTATTTGGACGAGCTGGCTGAAGGTGCAGATTTATAAGGAGTTGGCGATGTCAGTACCTCTCATTCTGACCATAc                                                 <  2:3496180/98‑1 (MQ=255)
                                                gATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACt                                                <  1:1290105/100‑1 (MQ=255)
                                                gATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACt                                                >  1:3273365/1‑100 (MQ=255)
                                                    aCAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCg                                            >  2:2580083/1‑100 (MQ=255)
                                                    aCAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCg                                            <  2:2120724/100‑1 (MQ=255)
                                                     cAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCgg                                           >  1:3176260/1‑100 (MQ=255)
                                                     cAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCgg                                           <  1:2755788/100‑1 (MQ=255)
                                                     cAATGATGATGATCATCAGTTATTTTGACGATCTCCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTATGACCATACTGGCgg                                           >  2:13910/1‑100 (MQ=255)
                                                      aaTGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCggg                                          <  2:447438/100‑1 (MQ=255)
                                                       atgatgatgATCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCgggg                                         >  2:89069/1‑100 (MQ=255)
                                                         gatgatgatCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGc                                       <  2:1191146/100‑1 (MQ=255)
                                                          atgatgatCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCa                                      <  2:869893/100‑1 (MQ=255)
                                                              tgatCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCa                                  >  1:3339305/1‑100 (MQ=255)
                                                               gatCATCAGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAc                                 >  2:838634/1‑100 (MQ=255)
                                                                  catcaGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGtt                              >  2:1191621/1‑100 (MQ=255)
                                                                  catcaGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGtt                              >  1:3497650/1‑100 (MQ=255)
                                                                    tcaGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTa                            <  1:955012/100‑1 (MQ=255)
                                                                     caGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTAt                           >  2:924822/1‑100 (MQ=255)
                                                                     caGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTAt                           >  2:486571/1‑100 (MQ=255)
                                                                     caGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTAt                           >  1:50144/1‑100 (MQ=255)
                                                                      aGTTATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGATTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGAGGGCAGCCACGTTTAtt                          >  2:2864012/1‑100 (MQ=255)
                                                                       ggtATTTTGACGATCTGCCTGAAGGTGCAGATTTATGAGGAGTTGTAGATGGCAGTACCTCTCATTCTGACCGTACTGGCGGGGGCAGCCACGTTTATTg                         <  2:2207210/98‑1 (MQ=255)
                                                                        ttATTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTgg                        <  2:1860873/100‑1 (MQ=255)
                                                                          aTTTTTACGAGCTGCCTTAAGGTGCATATTTATAATGAGTTGTCGATGTCAGAACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGgcg                      <  2:3018443/100‑1 (MQ=255)
                                                                          aTTTTGACGCTCTGCCAGAAGGTGCAGGTTTATAATGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGTCACGTCTTTTgcct                      >  2:3864738/1‑97 (MQ=255)
                                                                          aTTTTGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGgcg                      >  1:1565870/1‑100 (MQ=255)
                                                                           ttttGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGgcgc                     <  1:3948284/100‑1 (MQ=255)
                                                                            tttGACGATCTGCCTGAAGGTACAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGcgcg                    >  1:2565301/1‑100 (MQ=255)
                                                                             ttGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGt                   <  2:3683951/100‑1 (MQ=255)
                                                                              tGACGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGtt                  >  1:290321/1‑100 (MQ=255)
                                                                                aCGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTtc                >  2:1924769/1‑100 (MQ=255)
                                                                                aCGATCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTtc                <  1:2999577/100‑1 (MQ=255)
                                                                                   aTCTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTATGACCGTACTGGCGGGGGAAGCCAGGTTTATTGGCGCGTTTCTCg             >  1:3816344/1‑100 (MQ=255)
                                                                                     cTGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGc           <  2:635607/100‑1 (MQ=255)
                                                                                      tGCCTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCAGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCg          >  2:3818948/1‑100 (MQ=255)
                                                                                       gCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGt         >  1:3764822/1‑100 (MQ=255)
                                                                                        ccTGAAGGTGCAGATTTATAAGGAGGTGTCGATGTCAGTACCTCTCATTCTGACCATATTGGCGGGGAACGCCACGGTGATTGGCGCGTTTCTCGGCGtt        >  2:2290886/1‑100 (MQ=255)
                                                                                         cTGAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTtc       <  2:763526/100‑1 (MQ=255)
                                                                                           gAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTtctc     <  2:1056800/100‑1 (MQ=255)
                                                                                           gAAGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTtctc     <  2:1057327/100‑1 (MQ=255)
                                                                                             aGGTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGTGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCgg   <  2:1411934/100‑1 (MQ=255)
                                                                                              ggTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCggg  <  2:1375738/100‑1 (MQ=255)
                                                                                              ggTGCAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCggg  >  1:1099909/1‑100 (MQ=255)
                                                                                                  |                                                                                               
TCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGG  >  NC_000913/3182431‑3182624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: