Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,921 A→C E184A (GAA→GCA)  ttdB → L‑tartrate dehydratase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,9210AC91.7% 454.8 / 20.9 157E184A (GAA→GCA) ttdBL‑tartrate dehydratase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (8/5);  new base C (78/66);  total (86/71)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 159 total aligned reads displayed.

CTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCA  >  NC_000913/3207824‑3208016
                                                                                                 |                                                                                               
cTGTGGGTCTGCCGGGTCAAAGACTTCGGCCACCTCATTGTCTCTATTGATACTCACGGCAACAACCTGATAGCTGAAAACAAAAAGCTGTTCGCCGAAc                                                                                               <  2:1521089/100‑1 (MQ=255)
 tgtgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCAcg                                                                                              <  1:3613183/100‑1 (MQ=255)
 tgtgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCAcg                                                                                              <  1:2145092/100‑1 (MQ=255)
    gggTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCAcgccg                                                                                           >  1:2204903/1‑100 (MQ=255)
     ggTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCAcgccgc                                                                                          >  2:1540608/1‑100 (MQ=255)
      gTCTGCCGGGTAAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAAAAAAAAAAAAAAAAAGCTGTTCGCCGCACGCcgcg                                                                                         <  2:2794918/100‑1 (MQ=255)
         tGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATc                                                                                      >  1:1779119/1‑100 (MQ=255)
         tGCCGGGTCAAAGAGTTCGGCCCGCTGAATGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATc                                                                                      <  2:2923503/100‑1 (MQ=255)
          gCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACATAAAGCTGTTCGCCGCACGCCGCGATcc                                                                                     >  2:1795228/1‑100 (MQ=255)
            cGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCa                                                                                   >  2:2657113/1‑100 (MQ=255)
               gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAGCAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCg                                                                                >  2:3715194/1‑100 (MQ=255)
               gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCg                                                                                <  2:3399808/100‑1 (MQ=255)
               gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCg                                                                                >  2:1925559/1‑100 (MQ=255)
               gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCg                                                                                >  1:3803225/1‑100 (MQ=255)
                tCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGt                                                                               >  1:2132298/1‑100 (MQ=255)
                 cAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTg                                                                              <  2:3933380/100‑1 (MQ=255)
                 cAAAGAGTTCGGCCCGATGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTg                                                                              <  1:3353851/100‑1 (MQ=255)
                  aaaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTgg                                                                             <  1:2289942/100‑1 (MQ=255)
                     gagTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAag                                                                          >  2:1130785/1‑100 (MQ=255)
                       gTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAagag                                                                        >  1:2712562/1‑100 (MQ=255)
                         tCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGAt                                                                      <  2:2749094/100‑1 (MQ=255)
                          cGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATc                                                                     <  1:2024475/100‑1 (MQ=255)
                          cGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATc                                                                     <  1:1296262/100‑1 (MQ=255)
                          cGGCCCGCTGATTGTCGCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATc                                                                     <  1:2165450/100‑1 (MQ=255)
                           ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCt                                                                    >  1:1169480/1‑100 (MQ=255)
                            gCCCGCTGATTGTCTCTATGGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATAGTGGAAGAGATCTg                                                                   >  2:3661153/1‑100 (MQ=255)
                              ccGCTGATTGTGTCTATTGATAGCCGCGGCAACAACCTGATAGGCGAAAACAAAAAGGTGTTCGGCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                                                 <  2:2276647/100‑1 (MQ=255)
                                gCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAg                                                               >  1:3014853/1‑100 (MQ=255)
                                  tGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCa                                                             <  1:2760450/100‑1 (MQ=255)
                                   gATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                                            <  2:2171052/100‑1 (MQ=255)
                                   gATTGTCTCTAGTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                                            <  2:501788/100‑1 (MQ=255)
                                    aTTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCAGCGTGGAAGAGATCTGCGAGCACg                                                           >  1:1205081/1‑100 (MQ=255)
                                     ttGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGt                                                          >  2:641754/1‑100 (MQ=255)
                                     ttGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAAAGATCTGCGAGCACGt                                                          >  2:1429114/1‑100 (MQ=255)
                                      tGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTc                                                         >  1:301762/1‑100 (MQ=255)
                                        tctcTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCa                                                       <  1:2868396/100‑1 (MQ=255)
                                         ctctATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAt                                                      <  1:732994/100‑1 (MQ=255)
                                         ctctATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAt                                                      >  1:2106919/1‑100 (MQ=255)
                                          tctATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAtt                                                     >  2:2368400/1‑100 (MQ=255)
                                          tctATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAtt                                                     >  1:3860333/1‑100 (MQ=255)
                                           ctATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                                                    >  1:2597882/1‑100 (MQ=255)
                                            tATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTAc                                                   >  1:648964/1‑100 (MQ=255)
                                              ttGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAt                                                 <  2:1801302/100‑1 (MQ=255)
                                              ttGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAt                                                 <  2:1368867/100‑1 (MQ=255)
                                               tGATACCCACGGCAACAACCTGATAGCCGAAAACACAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATc                                                <  1:498382/100‑1 (MQ=255)
                                               tGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATc                                                >  1:2973833/1‑100 (MQ=255)
                                                gATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa                                               >  2:1057533/1‑100 (MQ=255)
                                                gATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa                                               <  2:1202829/100‑1 (MQ=255)
                                                 aTACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                                              <  2:3159645/100‑1 (MQ=255)
                                                  tACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa                                             >  2:232957/1‑100 (MQ=255)
                                                   aCCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaan                                            <  1:1948260/100‑2 (MQ=255)
                                                   aCCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCACAt                                            >  1:1630173/1‑100 (MQ=255)
                                                    cccACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCCAATa                                           >  1:511179/1‑100 (MQ=255)
                                                    cccACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa                                           >  2:3195874/1‑100 (MQ=255)
                                                    cccACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa                                           <  2:3013939/100‑1 (MQ=255)
                                                    cccACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa                                           <  1:1052435/100‑1 (MQ=255)
                                                     ccACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATaa                                          >  2:3085809/1‑100 (MQ=255)
                                                      cACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAc                                         <  1:350063/100‑1 (MQ=255)
                                                        cGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAccc                                       >  2:1004681/1‑100 (MQ=255)
                                                         ggCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCt                                      >  1:3519896/1‑100 (MQ=255)
                                                           caacacCCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCCCGGCGCGCTCCCGTCGTGGAAGAGATCTGCGAGCAAGTCCATTACATCAAATAACCCttc                                    <  2:2611947/100‑3 (MQ=255)
                                                             acaacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCTAATAACCCTCCCg                                  >  2:1898956/1‑100 (MQ=255)
                                                             acaacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCg                                  <  1:2217581/100‑1 (MQ=255)
                                                             acaacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCg                                  >  2:2680686/1‑100 (MQ=255)
                                                             acaacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCACCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCg                                  >  1:2968401/1‑100 (MQ=255)
                                                              caacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCgg                                 <  2:3512196/100‑1 (MQ=255)
                                                               aacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGga                                <  1:2299895/100‑1 (MQ=255)
                                                                 ccggATAGCCGAAAACAAAATGCTGTTCGCCGCGCGCCGCGAGCCCATCGTGGAAGAGATCTGCTAGCACGTCCATTACATCAAATAACCCTCCCGgaga                              <  2:2180375/97‑1 (MQ=255)
                                                                 ccTGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGgaga                              >  2:3720375/1‑100 (MQ=255)
                                                                   tGATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAgg                            >  2:3842486/1‑100 (MQ=255)
                                                                    gATAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc                           <  1:2370279/100‑1 (MQ=255)
                                                                      tAGCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCtn                         <  1:3253564/100‑2 (MQ=255)
                                                                        gCCGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAc                       >  2:1001594/1‑100 (MQ=255)
                                                                        gCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAc                       >  2:3868030/1‑100 (MQ=255)
                                                                        gCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAc                       >  1:196816/1‑100 (MQ=255)
                                                                         ccGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAcc                      <  1:2572527/100‑1 (MQ=255)
                                                                         ccGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAcc                      >  2:1482201/1‑100 (MQ=255)
                                                                          cGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGATAGGCTCAccc                     >  2:782075/1‑100 (MQ=255)
                                                                          cGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAccc                     >  1:1126424/1‑100 (MQ=255)
                                                                          cGAAAACAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGACCACGTCCATTACATCAAATAACCCTCCGGCAGACGCTCAccc                     >  2:3653106/1‑100 (MQ=255)
                                                                             aaaCAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCctc                  >  1:1077646/1‑100 (MQ=255)
                                                                                cAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTcc               >  2:516117/1‑100 (MQ=255)
                                                                                cAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTcc               >  1:859571/1‑100 (MQ=255)
                                                                                cAAAAAGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTAACCCCTCTcc               >  1:2536134/1‑100 (MQ=255)
                                                                                cAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTcc               >  2:1939625/1‑100 (MQ=255)
                                                                                 aaaaaGCTGTTCGCAGCCCGACGAGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCCAATAACCCTCCCGGAGAGGCTCACCCCTCTCCt              <  2:2478991/100‑1 (MQ=255)
                                                                                  aaaaGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCtt             >  2:468008/1‑100 (MQ=255)
                                                                                  aaaaGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCtt             >  1:3330291/1‑100 (MQ=255)
                                                                                  aaaaGCTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACACTCCCGGAGAGGCTCACCCCTCTCCtt             >  2:2776985/1‑100 (MQ=255)
                                                                                    caGCTGTCCTGCGCACGCCGCGAGCCCATCTTTCGAGAGGTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCtttt           <  2:3267719/99‑1 (MQ=255)
                                                                                      gCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTc         <  2:3756968/100‑1 (MQ=255)
                                                                                       cTGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCg        <  2:1283767/100‑1 (MQ=255)
                                                                                        tGTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGc       >  1:1512276/1‑100 (MQ=255)
                                                                                         gTTCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCa      >  1:65203/1‑100 (MQ=255)
                                                                                          ttCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAg     <  1:2091768/100‑1 (MQ=255)
                                                                                          ttCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAg     <  2:2671320/100‑1 (MQ=255)
                                                                                           tCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAgg    <  1:2323844/100‑1 (MQ=255)
                                                                                           tCGCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAgg    <  2:405075/100‑1 (MQ=255)
                                                                                            cgccgcACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGc   <  2:3254498/100‑1 (MQ=255)
                                                                                             gCCGCACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCa  >  2:693426/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCA  >  NC_000913/3207824‑3208016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑