Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,290,794 A→C I661L (ATC→CTC)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,290,7940AC98.7% 512.5 / ‑3.0 151I661L (ATC→CTC) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (70/77);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 169 total aligned reads displayed.

CGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTAT  >  NC_000913/3290697‑3290889
                                                                                                 |                                                                                               
cGCCAGTTACCGTTCACCTTATGGCCCCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTtctc                                                                                               <  2:1187676/100‑1 (MQ=255)
cGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTtctc                                                                                               <  1:2039747/100‑1 (MQ=255)
 gCCAGGTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcc                                                                                              >  2:3517586/1‑100 (MQ=255)
  ncAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcca                                                                                             >  1:3235692/2‑100 (MQ=255)
  ccAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcca                                                                                             <  1:280139/100‑1 (MQ=255)
  ccAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcca                                                                                             <  1:2659586/100‑1 (MQ=255)
  ccAGGTACCGTGCACCGTATGGCACCGTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctcca                                                                                             <  2:1318291/100‑1 (MQ=255)
   cAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccat                                                                                            <  2:1055790/100‑1 (MQ=255)
     gTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatcc                                                                                          <  2:1766531/100‑1 (MQ=255)
     gTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatcc                                                                                          >  2:2895884/1‑100 (MQ=255)
     gTTACCGTCCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTctccatcc                                                                                          >  1:3880936/1‑100 (MQ=255)
      ttACCGTTCACCTTACGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCt                                                                                         <  2:2932624/100‑1 (MQ=255)
        aCCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCa                                                                                       <  2:2122820/100‑1 (MQ=255)
         ccGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCAt                                                                                      <  1:2779872/100‑1 (MQ=255)
          cGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATg                                                                                     >  1:1068839/1‑100 (MQ=255)
           gTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATgg                                                                                    >  1:628438/1‑100 (MQ=255)
            ttCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGc                                                                                   >  2:2160729/1‑100 (MQ=255)
               aCCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTg                                                                                <  2:2822918/100‑1 (MQ=255)
                ctttATGGTACCTTCAGTGCCGGATACAGCTACGGTATTGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                               <  2:3617299/98‑1 (MQ=255)
                ccTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                               <  2:1336168/100‑1 (MQ=255)
                ccTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                               <  1:2549037/100‑1 (MQ=255)
                ccTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGa                                                                               >  1:2176522/1‑100 (MQ=255)
                 cTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGAc                                                                              <  1:301838/100‑1 (MQ=255)
                 cTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGAc                                                                              <  1:3008443/100‑1 (MQ=255)
                 cTTATGGCACCTTCAGTGAAGGATACAGCTACGGTAATGACATGAGACAATACAATTAAGTTGTTATCGGGGGAGAGGTCTTCCAGCCTCATGGAGTGaa                                                                              >  2:1800660/1‑99 (MQ=255)
                   tATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGc                                                                            >  1:3417122/1‑100 (MQ=255)
                    aTGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGct                                                                           <  1:2656833/100‑1 (MQ=255)
                      ggCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGAGGTTCTCCATCCTCATGGCGTGACGctct                                                                         >  1:3728902/1‑100 (MQ=255)
                       gCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCtctc                                                                        >  1:1011212/1‑100 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                       <  2:306943/100‑1 (MQ=255)
                        cACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCg                                                                       <  1:1275465/100‑1 (MQ=255)
                         aCCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCCCTCGc                                                                      >  2:1131507/1‑100 (MQ=255)
                            ttCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAat                                                                   >  1:1308820/1‑100 (MQ=255)
                            ttCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAat                                                                   >  1:3036816/1‑100 (MQ=255)
                             tCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAata                                                                  <  2:410498/100‑1 (MQ=255)
                              cAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAatat                                                                 >  1:1794897/1‑100 (MQ=255)
                                gTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCt                                                               >  1:3643239/1‑100 (MQ=255)
                                gTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCt                                                               >  2:1535614/1‑100 (MQ=255)
                                 tGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTg                                                              >  1:208379/1‑100 (MQ=255)
                                   ccGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTggg                                                            <  2:2991724/100‑1 (MQ=255)
                                   ccGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTggg                                                            <  1:3668967/100‑1 (MQ=255)
                                        tACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACg                                                       >  1:199230/1‑100 (MQ=255)
                                          cAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCt                                                     >  1:42440/1‑100 (MQ=255)
                                           aGCGACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATCTCTGGGCAACGCtt                                                    >  2:3664121/1‑100 (MQ=255)
                                            gCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCttt                                                   >  2:737049/1‑100 (MQ=255)
                                            gCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCttt                                                   >  1:1943493/1‑100 (MQ=255)
                                              tACGGTAATGACAGCAGCCAATAGAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTg                                                 <  1:1505936/100‑1 (MQ=255)
                                              tACGGTAATGACAGCAGCCAATACAATTACGCCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTg                                                 <  2:410505/100‑1 (MQ=255)
                                               aCGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTgc                                                <  1:1137217/100‑1 (MQ=255)
                                                cGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATACTCATGGCGTGACGATCTCGCAATATCTGGGCCACGCTTTTgcg                                               >  1:3300435/1‑100 (MQ=255)
                                                  gTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCt                                             >  1:1934353/1‑100 (MQ=255)
                                                  gTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCt                                             <  2:1996282/100‑1 (MQ=255)
                                                  gTAATAACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGAGACGCCCTCGCACTTTCTGGGCAACGCTTTTGCGCt                                             >  1:2564320/1‑100 (MQ=255)
                                                   tAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCtt                                            >  2:393721/1‑100 (MQ=255)
                                                    aaTGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCCACGCTTTTGCGCTTa                                           >  2:2206894/1‑100 (MQ=255)
                                                     ntGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTAt                                          >  1:1018521/2‑100 (MQ=255)
                                                      tGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATc                                         <  1:911105/100‑1 (MQ=255)
                                                      tGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATc                                         <  1:1525780/100‑1 (MQ=255)
                                                      tGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATc                                         <  2:312669/100‑1 (MQ=255)
                                                       gACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCg                                        <  2:3680530/100‑1 (MQ=255)
                                                          agcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATg                                     <  1:2110883/100‑1 (MQ=255)
                                                           gcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGc                                    >  2:2808009/1‑100 (MQ=255)
                                                           gcagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGc                                    <  2:2375692/100‑1 (MQ=255)
                                                            cagcCAATACAATTACGGCGTTACCTGCTGCTTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATGTGGGCAACGCTTTTGCTCTTATCGATGCt                                   <  2:3643821/100‑1 (MQ=255)
                                                            cagcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCt                                   <  1:2830107/100‑1 (MQ=255)
                                                             agcCAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTa                                  >  1:2222570/1‑100 (MQ=255)
                                                               ccAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAAc                                <  2:1643734/100‑1 (MQ=255)
                                                                cAATACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACg                               >  2:555419/1‑100 (MQ=255)
                                                                   tACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAg                            <  2:2398753/100‑1 (MQ=255)
                                                                   tACAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAg                            <  1:704465/100‑1 (MQ=255)
                                                                    aCAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGc                           <  2:2110083/100‑1 (MQ=255)
                                                                    aCAATTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGc                           <  1:3596450/100‑1 (MQ=255)
                                                                      aaTTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCAt                         >  2:487513/1‑100 (MQ=255)
                                                                      aaTTACGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCAt                         >  1:1529748/1‑100 (MQ=255)
                                                                           cGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGc                    >  1:2005241/1‑100 (MQ=255)
                                                                           cGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGc                    >  1:3819073/1‑100 (MQ=255)
                                                                           cGGCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGc                    >  2:547990/1‑100 (MQ=255)
                                                                            ggCGTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCg                   <  1:373904/100‑1 (MQ=255)
                                                                               gTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGa                >  1:1174188/1‑100 (MQ=255)
                                                                               gTTACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGa                >  1:1574485/1‑100 (MQ=255)
                                                                                ttACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAg               <  1:51951/100‑1 (MQ=255)
                                                                                ttACCGGCGGCATGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTCGCGATGCTAACGGAACATCTGGCGTGAg               <  1:3595023/100‑1 (MQ=255)
                                                                                 tACCGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGGTGCTAACGGAGCATCTGGCGTGAgg              <  2:3185609/100‑1 (MQ=255)
                                                                                   ccGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGAt            >  1:359382/1‑100 (MQ=255)
                                                                                   ccGGCGGCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGAt            >  2:3316716/1‑100 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa           <  2:2519405/100‑1 (MQ=255)
                                                                                    cggcggCGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATa           >  1:1654784/1‑100 (MQ=255)
                                                                                     ggcggcGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATAc          <  1:2391301/100‑1 (MQ=255)
                                                                                     ggcggcGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATAc          >  1:3275462/1‑100 (MQ=255)
                                                                                     ggcggcGTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATAc          >  2:3613045/1‑100 (MQ=255)
                                                                                       cggcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACACTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaa        <  1:3085435/100‑1 (MQ=255)
                                                                                        ggcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaaa       >  1:1038861/1‑100 (MQ=255)
                                                                                        ggcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaaa       <  1:2220530/100‑1 (MQ=255)
                                                                                         gcgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACaaaa      <  2:1903593/100‑1 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc     >  2:80921/1‑100 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc     <  2:1574469/100‑1 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc     <  1:685167/100‑1 (MQ=255)
                                                                                          cgTGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAAc     <  2:1032958/100‑1 (MQ=255)
                                                                                            tGGTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTa   >  1:3339985/1‑100 (MQ=255)
                                                                                             ggTTCTCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTAt  <  1:497092/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGCCAGTTACCGTTCACCTTATGGCACCTTCAGTGCCGGATACAGCTACGGTAATGACAGCAGCCAATACAATTACGGCGTTACCGGCGGCGTGGTTATCCATCCTCATGGCGTGACGCTCTCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTAT  >  NC_000913/3290697‑3290889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: