Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,444,566 A→C F53V (TTC→GTC)  rpmD ← 50S ribosomal subunit protein L30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,444,5660AC97.9% 488.7 / ‑1.7 144F53V (TTC→GTC) rpmD50S ribosomal subunit protein L30
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (75/65);  total (76/68)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 144 total aligned reads displayed.

CGATACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGAAGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAAGCAGCGTTGCC  >  NC_000913/3444470‑3444660
                                                                                                |                                                                                              
cGATACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGGATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACggt                                                                                             >  1:2879254/1‑99 (MQ=255)
 gATACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGaa                                                                                            >  1:1050754/1‑100 (MQ=255)
 gATACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGaa                                                                                            >  1:1125078/1‑100 (MQ=255)
   tACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAAc                                                                                          <  2:2330050/100‑1 (MQ=255)
     ccACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACcg                                                                                        >  2:1599977/1‑100 (MQ=255)
     ccACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACcg                                                                                        <  2:2820790/100‑1 (MQ=255)
         gACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGtt                                                                                    <  2:2023012/100‑1 (MQ=255)
         gACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGtt                                                                                    <  2:1803751/100‑1 (MQ=255)
          aCCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACCTTAACCATGACGGAAACCGCGTTg                                                                                   <  2:1684437/100‑1 (MQ=255)
           cccAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGATGGAAACCGCGTTGa                                                                                  <  2:3095484/100‑1 (MQ=255)
           cccAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGa                                                                                  >  1:1127248/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGGGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                >  1:102522/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                >  2:3402059/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                <  2:2705097/100‑1 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                >  1:1700769/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                <  1:769398/100‑1 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                <  1:586174/100‑1 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                >  1:110879/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                >  1:2834503/1‑100 (MQ=255)
             cAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGAAAAAAAAAAAATATATAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATc                                                                                <  2:763596/100‑1 (MQ=255)
               ggCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCAt                                                                              >  2:3852384/1‑100 (MQ=255)
               ggCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCAt                                                                              <  2:127373/100‑1 (MQ=255)
                 cGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAAGTTTAACCATGACGGAAACCGCGTTGATCATAc                                                                            >  1:3737734/1‑100 (MQ=255)
                  gtctACCCGCCTGTTTGGAGCCTTCGGACGGAGACAGAGTATTTCAACGCAACTCTTACTCCTCAACTTTTTCCATGACGGAAACCGCGTTGATCATAcc                                                                           <  2:1727850/97‑1 (MQ=255)
                  gTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATAcc                                                                           <  2:3496940/100‑1 (MQ=255)
                  gTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATAcc                                                                           >  1:2196909/1‑100 (MQ=255)
                  gTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATAcc                                                                           >  2:475945/1‑100 (MQ=255)
                  gTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATAcc                                                                           >  1:1915529/1‑100 (MQ=255)
                  gTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATAcc                                                                           <  1:3050760/100‑1 (MQ=255)
                   tttACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACcg                                                                          <  2:3476354/100‑1 (MQ=255)
                    ttACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATTCcgc                                                                         <  2:3386327/100‑1 (MQ=255)
                      aCCCGCCTGTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTCCTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGa                                                                       >  2:3382994/1‑100 (MQ=255)
                       cccGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGaa                                                                      >  2:911299/1‑100 (MQ=255)
                       cccGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGaa                                                                      >  2:2907719/1‑100 (MQ=255)
                        ccGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTCTTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAAt                                                                     >  2:3692229/1‑100 (MQ=255)
                        ccGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAAt                                                                     <  2:1056814/100‑1 (MQ=255)
                        ccGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAAt                                                                     >  1:3010475/1‑100 (MQ=255)
                         cGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATa                                                                    >  1:203271/1‑100 (MQ=255)
                           ccTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGc                                                                  <  2:1631463/100‑1 (MQ=255)
                            cTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCa                                                                 <  1:1870606/100‑1 (MQ=255)
                               tttGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGa                                                              <  1:3106714/100‑1 (MQ=255)
                               tttGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGa                                                              <  1:2259416/100‑1 (MQ=255)
                                  ggAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGCTGATCATACCGCGAATAGCAGGAGTa                                                           >  2:3949066/1‑100 (MQ=255)
                                   gAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTAt                                                          >  1:767818/1‑100 (MQ=255)
                                    aGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATc                                                         <  2:3114172/100‑1 (MQ=255)
                                     gCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATcc                                                        >  1:229185/1‑100 (MQ=255)
                                      ccTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATccn                                                       <  1:3252412/100‑2 (MQ=255)
                                          cGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTcgcg                                                   <  2:3603246/100‑1 (MQ=255)
                                          cGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTcgcg                                                   <  1:1704475/100‑1 (MQ=255)
                                          cGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTcgcg                                                   <  1:2299187/100‑1 (MQ=255)
                                           ggCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCgcgc                                                  >  1:2089303/1‑100 (MQ=255)
                                            gCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGct                                                 >  2:379812/1‑100 (MQ=255)
                                            gCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGct                                                 <  2:107511/100‑1 (MQ=255)
                                             ccGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGctc                                                >  1:2473825/1‑100 (MQ=255)
                                             ccGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGctc                                                >  1:2123777/1‑100 (MQ=255)
                                                gagaCAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTAc                                             <  1:3078271/100‑1 (MQ=255)
                                                    cAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGgtg                                         <  2:64236/100‑1 (MQ=255)
                                                    cAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGgtg                                         >  1:1918136/1‑100 (MQ=255)
                                                    cAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGAAGGAGTCTCCTCGCGCTCTACGgtg                                         >  1:2613998/1‑100 (MQ=255)
                                                     agagTATTTAAACGCATCTCTTACTCCTCAACTTTCACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGgtgt                                        <  1:2233160/100‑1 (MQ=255)
                                                     agagTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGgtgt                                        <  2:420123/100‑1 (MQ=255)
                                                      gagTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGtgtg                                       >  2:3744400/1‑100 (MQ=255)
                                                      gagTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGtgtg                                       >  2:1990598/1‑100 (MQ=255)
                                                       agTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGa                                      <  2:2585519/100‑1 (MQ=255)
                                                       agTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGa                                      >  1:21687/1‑100 (MQ=255)
                                                         tATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGAcc                                    <  2:2791739/100‑1 (MQ=255)
                                                         tATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGAcc                                    <  1:930901/100‑1 (MQ=255)
                                                          aTTTAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCa                                   <  2:955546/100‑1 (MQ=255)
                                                           tttAACCGCATCTCTTACTCCTCAACTTTAACCATGACGGCAACCGCCTTGATCATACCTCGAATATCAGGAGTATCCTCGCGCTCTACGGTGTCACCaa                                  >  2:1279027/1‑100 (MQ=255)
                                                           tttAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCaa                                  >  2:2380928/1‑100 (MQ=255)
                                                            ttAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAAt                                 >  1:362311/1‑100 (MQ=255)
                                                            ttAAACGCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAAt                                 <  1:3934346/100‑1 (MQ=255)
                                                                  gCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATacgatg                           <  1:2124880/100‑3 (MQ=255)
                                                                  gCATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCATTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATacgacg                           >  1:440776/1‑100 (MQ=255)
                                                                   cATCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGc                          >  1:1626079/1‑100 (MQ=255)
                                                                   cATCTCTTACTCCGCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGc                          <  1:3675653/100‑1 (MQ=255)
                                                                    aTCTCTTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCa                         <  1:356177/100‑1 (MQ=255)
                                                                       tctTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCCCGCTCTACGGTGTGACCAATACGACGCAgcc                      >  2:3594910/1‑98 (MQ=255)
                                                                        ctTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCCTACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGAcc                     >  2:3642820/1‑100 (MQ=255)
                                                                        ctTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGAcc                     <  1:4046166/100‑1 (MQ=255)
                                                                        ctTACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGAcc                     >  1:379546/1‑100 (MQ=255)
                                                                         ttACTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGAccc                    >  2:1575392/1‑100 (MQ=255)
                                                                           aCTCCTCAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAg                  >  2:812890/1‑100 (MQ=255)
                                                                            ctcctcAACTTTAACCATGACGGAAACCGCGTTGCTCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAgg                 <  1:2441446/100‑1 (MQ=255)
                                                                            ctcctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAgg                 >  2:478799/1‑100 (MQ=255)
                                                                            ctcctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAgg                 >  2:2869635/1‑100 (MQ=255)
                                                                              cctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGcc               <  1:1337813/100‑1 (MQ=255)
                                                                              cctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGcc               >  2:2524213/1‑100 (MQ=255)
                                                                              cctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGcc               >  2:1434835/1‑100 (MQ=255)
                                                                              cctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGcc               >  1:3364402/1‑100 (MQ=255)
                                                                               ctcAACTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCACACCCAGGCCa              >  2:3497092/1‑100 (MQ=255)
                                                                                 cAACTTTAACCATGACGGACACCGCGTTGATCATACCGCGAATAGCAGGCGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAag            >  1:2264643/1‑100 (MQ=255)
                                                                                  aaCTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAagc           <  1:590745/100‑1 (MQ=255)
                                                                                   aCTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCCGACCCAGGCCAagca          >  2:402314/1‑100 (MQ=255)
                                                                                   aCTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAagca          >  1:997475/1‑100 (MQ=255)
                                                                                    cTTTAACCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCCCTACGGTGTGACCAATACGACGCAGACCCAGGCCAagcag         >  1:1275640/1‑100 (MQ=255)
                                                                                      ttAACCATGGCGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAAGCAGCg       <  2:103003/100‑1 (MQ=255)
                                                                                         aCCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTTACCAATACGACGCAGACCCAGGCCAAGCAGCGTTg    >  2:539562/1‑100 (MQ=255)
                                                                                         aCCATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAAGCAGCGTTg    <  1:1627223/100‑1 (MQ=255)
                                                                                           cATGACGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAAGCAGCGTTGcc  <  2:3262073/100‑1 (MQ=255)
                                                                                                |                                                                                              
CGATACCACGACCCAGGCGTTTACCCGCCTTTTTGGAGCCTTCGGCCGGAGACAGAGTATTTAAACGCATCTCTTACTCCTCAACTTTAACCATGAAGGAAACCGCGTTGATCATACCGCGAATAGCAGGAGTATCCTCGCGCTCTACGGTGTGACCAATACGACGCAGACCCAGGCCAAGCAGCGTTGCC  >  NC_000913/3444470‑3444660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑