Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 299,214 A→C F574C (TTC→TGC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913299,2140AC98.0% 505.7 / 0.8 148F574C (TTC→TGC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (80/65);  total (82/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 148 total aligned reads displayed.

CGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACT  >  NC_000913/299118‑299310
                                                                                                |                                                                                                
cGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCAtt                                                                                               >  1:173482/1‑100 (MQ=255)
  ccGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCa                                                                                             >  1:1414018/1‑100 (MQ=255)
    ggACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAAt                                                                                           <  1:2918930/100‑1 (MQ=255)
        ccGAGCTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctc                                                                                       <  1:4065370/100‑1 (MQ=255)
        ccGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctn                                                                                       <  1:3260452/100‑2 (MQ=255)
         cGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctct                                                                                      >  1:477075/1‑100 (MQ=255)
         cGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctct                                                                                      <  2:1774358/100‑1 (MQ=255)
         cGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctct                                                                                      >  2:2508772/1‑100 (MQ=255)
         cGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctct                                                                                      >  1:2232264/1‑100 (MQ=255)
             cTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTc                                                                                  >  2:736951/1‑100 (MQ=255)
              ttCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTcc                                                                                 <  2:2653683/100‑1 (MQ=255)
               tctcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCg                                                                                >  1:1325787/1‑100 (MQ=255)
                 tcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCt                                                                              >  2:3380268/1‑100 (MQ=255)
                  cccGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTg                                                                             <  1:856871/100‑1 (MQ=255)
                  cccGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTg                                                                             <  2:2896443/100‑1 (MQ=255)
                    cGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTc                                                                           >  2:3729279/1‑100 (MQ=255)
                     gTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCa                                                                          >  1:2661408/1‑100 (MQ=255)
                      tCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAg                                                                         <  1:3920088/100‑1 (MQ=255)
                          gcTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTg                                                                     >  2:1899886/1‑100 (MQ=255)
                            tATGCACGCCGACCTACACAAAATGCTCGGCAAAGCTCGACTGCTGGTACTATCTGCTAAGTCTTCCGCATTCACAGCTCGCTTCCGCTCCCAGtgttcc                                                                   >  2:3625167/1‑95 (MQ=255)
                              tGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTgccgc                                                                 >  2:3536740/1‑100 (MQ=255)
                               gCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTgccgcc                                                                >  2:1582635/1‑100 (MQ=255)
                                 aCGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTg                                                              >  1:3709645/1‑100 (MQ=255)
                                  cGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGc                                                             >  1:1670107/1‑100 (MQ=255)
                                  cGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGc                                                             >  2:1490974/1‑100 (MQ=255)
                                  cGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGc                                                             <  1:143918/100‑1 (MQ=255)
                                  cGCCGACCTCCACAAAAGGCCCGGCAAAGTTGGACGGCGGGTCCTTTTTTCGCAGTGTTCCGCATTCAATGCTCTCTTCTGCTGTCAGTCTGCCGCCTGc                                                             <  2:3991885/100‑1 (MQ=255)
                                   gCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCg                                                            <  1:801962/100‑1 (MQ=255)
                                      gACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTg                                                         <  2:1642338/100‑1 (MQ=255)
                                      gACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTg                                                         <  1:1706248/100‑1 (MQ=255)
                                        ccTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGTCGCCTGCGGTGGc                                                       <  1:760488/100‑1 (MQ=255)
                                        ccTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGc                                                       >  2:3138385/1‑100 (MQ=255)
                                        ccTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGc                                                       >  1:2360423/1‑100 (MQ=255)
                                         cTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCt                                                      >  1:3442921/1‑100 (MQ=255)
                                         cTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCt                                                      <  1:3990623/100‑1 (MQ=255)
                                          tCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCtt                                                     >  2:1358266/1‑100 (MQ=255)
                                           ccACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTGCGCATTCAATGCTCTCTTCCACTGTCAGTCTGCCGCCTGCGGTGGCTTc                                                    >  2:3936599/1‑100 (MQ=255)
                                            cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  2:1342942/100‑1 (MQ=255)
                                            cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  2:915599/100‑1 (MQ=255)
                                            cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  1:2373144/100‑1 (MQ=255)
                                              caAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCTCTGTCAGTCTGCCGCCTGCGGTGGCTTCATg                                                 <  2:72827/100‑1 (MQ=255)
                                              caAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATg                                                 >  1:3118604/1‑100 (MQ=255)
                                              caAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATg                                                 >  1:253203/1‑100 (MQ=255)
                                              caAAATGCCCGGCAAAGGTCGACTACTGCTACTCTTTGCTCAGTGTTCCGCCTTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATg                                                 <  2:2609658/100‑1 (MQ=255)
                                                aaaTGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTa                                               >  2:347001/1‑100 (MQ=255)
                                                     cccGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTg                                          >  1:3572143/1‑100 (MQ=255)
                                                      ccGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTgg                                         <  2:2378722/100‑1 (MQ=255)
                                                       cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        <  2:2620871/100‑1 (MQ=255)
                                                       cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        >  2:1309653/1‑100 (MQ=255)
                                                       cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        >  2:1101685/1‑100 (MQ=255)
                                                       cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        <  1:2290237/100‑1 (MQ=255)
                                                       cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        >  2:558392/1‑100 (MQ=255)
                                                       cGGCAAAGGCCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCATCCGCTGGCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        <  2:3048460/100‑1 (MQ=255)
                                                          cAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCt                                     >  2:1715171/1‑100 (MQ=255)
                                                           aaaGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGCCTTCATGTAGCGTGGCGCtt                                    <  2:1018896/100‑1 (MQ=255)
                                                            aaGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTc                                   >  2:3362120/1‑100 (MQ=255)
                                                              ggTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCgg                                 <  2:2884150/100‑1 (MQ=255)
                                                               gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                <  1:3483714/100‑1 (MQ=255)
                                                               gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                <  1:1554163/100‑1 (MQ=255)
                                                               gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                >  1:3070105/1‑100 (MQ=255)
                                                               gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                >  1:1201654/1‑100 (MQ=255)
                                                               gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                <  2:2052449/100‑1 (MQ=255)
                                                                 cGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTa                              <  1:3798447/100‑1 (MQ=255)
                                                                   aCTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTAcc                            <  1:1248469/100‑1 (MQ=255)
                                                                   aCTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTAcc                            <  2:1669012/100‑1 (MQ=255)
                                                                      gctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGtt                         >  1:2432580/1‑100 (MQ=255)
                                                                      gctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTCCGGGTACCGtt                         >  2:3191674/1‑100 (MQ=255)
                                                                       ctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTg                        <  2:22410/100‑1 (MQ=255)
                                                                       ctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTg                        <  1:1953444/100‑1 (MQ=255)
                                                                        tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       >  2:872021/1‑100 (MQ=255)
                                                                        tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       <  1:1050398/100‑1 (MQ=255)
                                                                        tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       >  1:3442988/1‑100 (MQ=255)
                                                                        tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       <  2:338141/100‑1 (MQ=255)
                                                                         ggTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGt                      >  1:2173735/1‑100 (MQ=255)
                                                                         ggTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGt                      <  2:3063688/100‑1 (MQ=255)
                                                                          gTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTa                     <  2:2951326/100‑1 (MQ=255)
                                                                            aCTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAAt                   >  1:3955935/1‑100 (MQ=255)
                                                                            aCTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAAt                   <  1:3463225/100‑1 (MQ=255)
                                                                             ctctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATc                  >  1:4008200/1‑100 (MQ=255)
                                                                             cgctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATc                  <  1:3690203/98‑1 (MQ=255)
                                                                              tctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCt                 >  2:3794291/1‑100 (MQ=255)
                                                                              tctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCt                 >  1:4019944/1‑100 (MQ=255)
                                                                               ctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCtt                >  2:2333002/1‑100 (MQ=255)
                                                                                tttGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTg               <  1:3165736/100‑1 (MQ=255)
                                                                                tttGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTg               >  2:2005936/1‑100 (MQ=255)
                                                                                tttGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTg               >  2:2716039/1‑100 (MQ=255)
                                                                                    cTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGt           >  2:670022/1‑100 (MQ=255)
                                                                                    cTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGt           >  1:355649/1‑100 (MQ=255)
                                                                                    cTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGt           >  1:907306/1‑100 (MQ=255)
                                                                                      cAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCg         <  1:2852779/100‑1 (MQ=255)
                                                                                      cAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCg         >  1:331476/1‑100 (MQ=255)
                                                                                       aGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCgg        <  1:1347564/100‑1 (MQ=255)
                                                                                       aGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCgg        <  2:3357945/100‑1 (MQ=255)
                                                                                       aGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCgg        <  1:3906698/100‑1 (MQ=255)
                                                                                         tgtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCa      <  1:1034106/100‑1 (MQ=255)
                                                                                          gtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCaa     >  1:1004901/1‑100 (MQ=255)
                                                                                            tCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAAc   >  1:4019362/1‑100 (MQ=255)
                                                                                            tCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAAc   >  2:473744/1‑100 (MQ=255)
                                                                                            tCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAAc   >  1:2078678/1‑100 (MQ=255)
                                                                                             ccGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACt  >  2:1608732/1‑100 (MQ=255)
                                                                                                |                                                                                                
CGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACT  >  NC_000913/299118‑299310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: