Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,473,6120AC98.9% 628.3 / ‑4.7 182L157* (TTA→TGA) rpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (96/84);  total (97/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 182 total aligned reads displayed.

TTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAG  >  NC_000913/3473515‑3473708
                                                                                                 |                                                                                                
 tGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCAcc                                                                                               <  2:408302/100‑1 (MQ=255)
  gTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCa                                                                                              >  2:2579296/1‑100 (MQ=255)
   tttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCaa                                                                                             >  2:66016/1‑100 (MQ=255)
      ccTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACgg                                                                                          <  1:656914/100‑1 (MQ=255)
       cTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGt                                                                                         >  1:4022165/1‑100 (MQ=255)
         cGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAg                                                                                       <  2:1755637/100‑1 (MQ=255)
         cGTTTAACTTTTACGCTTTCAATTAAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAg                                                                                       <  1:1018499/100‑1 (MQ=255)
          gTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgt                                                                                      >  1:412509/1‑100 (MQ=255)
           tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtg                                                                                     >  2:3110858/1‑100 (MQ=255)
           tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtg                                                                                     >  1:984768/1‑100 (MQ=255)
           tttATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAgtg                                                                                     <  1:1852049/100‑1 (MQ=255)
           tttAGCGTTTAGGCGTTCAATGTAAGTAGCGCAAAGCGGGATGCTTACTGGAAGCGCCCGCCTGGTGACTAAGGCTCGGAAGGGATAACCAGCGGTAgtg                                                                                     <  2:3545256/100‑1 (MQ=255)
             tATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTGAAACTCCGAAGGGATCACCAACGGTAGtgtg                                                                                   <  1:3276729/100‑1 (MQ=255)
             tATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGtgtg                                                                                   <  2:960561/100‑1 (MQ=255)
                cTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGa                                                                                <  2:3219347/100‑1 (MQ=255)
                cTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGa                                                                                >  1:2161103/1‑100 (MQ=255)
                 ttttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGaa                                                                               >  1:4039095/1‑100 (MQ=255)
                 ttttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGaa                                                                               >  1:1033761/1‑100 (MQ=255)
                     aGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGcc                                                                           >  2:3253620/1‑100 (MQ=255)
                        cGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCACCGGTAGTGTGCGAACGCCttg                                                                        >  2:1799732/1‑100 (MQ=255)
                        cGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttg                                                                        >  2:3270302/1‑100 (MQ=255)
                         gTTCAATTTAAGTTGCCCAAAGCGGGCTGCTGACTGGAAGCGCACGCCTGGGGACTAAAACTTCGAAGCCATCATCAACGGTAGTGTGCGAACGCCttgt                                                                       <  2:2802158/100‑1 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       <  2:1246790/100‑1 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  2:3604539/1‑100 (MQ=255)
                         gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgt                                                                       >  1:1589996/1‑100 (MQ=255)
                          ttCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgtt                                                                      >  1:109258/1‑100 (MQ=255)
                          ttCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgtt                                                                      <  2:1558749/100‑1 (MQ=255)
                           tCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCttgttg                                                                     <  2:1443954/100‑1 (MQ=255)
                            cAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTgg                                                                    <  1:2857667/100‑1 (MQ=255)
                            cAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTgg                                                                    >  2:3510124/1‑100 (MQ=255)
                            cAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTgg                                                                    <  1:2165905/100‑1 (MQ=255)
                             naTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGc                                                                   >  1:3257094/2‑100 (MQ=255)
                             aaTTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGc                                                                   >  2:3168741/1‑100 (MQ=255)
                               tttAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCtt                                                                 >  1:3383236/1‑100 (MQ=255)
                               tttAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCtt                                                                 <  1:1945370/100‑1 (MQ=255)
                                ttAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTc                                                                >  2:2736823/1‑100 (MQ=255)
                                 tAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCg                                                               <  1:3586963/100‑1 (MQ=255)
                                  aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                              <  1:1231676/100‑1 (MQ=255)
                                  aaGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCgg                                                              <  1:935804/100‑1 (MQ=255)
                                   aGTAGGCCAAGGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGc                                                             <  1:1873667/100‑1 (MQ=255)
                                   aGTAGCCCAAAGCGTGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGc                                                             <  2:3663326/100‑1 (MQ=255)
                                    gTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            >  1:3202239/1‑100 (MQ=255)
                                    gTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            <  1:2722198/100‑1 (MQ=255)
                                    gTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGcc                                                            >  2:1128861/1‑100 (MQ=255)
                                      aGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCAt                                                          >  1:2376852/1‑100 (MQ=255)
                                      aGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCAt                                                          <  1:1171357/100‑1 (MQ=255)
                                      aGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCAt                                                          <  1:2197245/100‑1 (MQ=255)
                                      aGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCAt                                                          >  1:404597/1‑100 (MQ=255)
                                       gCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATa                                                         >  2:2971910/1‑100 (MQ=255)
                                        cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATAc                                                        <  1:1321749/100‑1 (MQ=255)
                                         ccAAAGCGGGCTGCTTACTGGCAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                       >  1:3482809/1‑100 (MQ=255)
                                          cAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACgg                                                      >  2:2360223/1‑100 (MQ=255)
                                          cAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACgg                                                      <  2:597392/100‑1 (MQ=255)
                                           aaaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                     <  1:2255534/100‑1 (MQ=255)
                                            aaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                    <  2:210196/100‑1 (MQ=255)
                                            aaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                    <  2:334648/100‑1 (MQ=255)
                                              gCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGaa                                                  <  2:3736637/100‑1 (MQ=255)
                                               cGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                 <  2:109455/100‑1 (MQ=255)
                                                  gctgctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTc                                              >  1:1143251/1‑100 (MQ=255)
                                                    tgctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCtt                                            >  2:3651220/1‑100 (MQ=255)
                                                      catACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCa                                          <  2:3974362/98‑1 (MQ=255)
                                                        tACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACg                                        <  1:618682/100‑1 (MQ=255)
                                                        tACTGGAAGCGCCCGCATGGTGACTAAAACTCCGAAGGGATCACGAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACg                                        >  1:3574116/1‑100 (MQ=255)
                                                         aCTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGt                                       >  1:1714116/1‑100 (MQ=255)
                                                          cTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                      >  2:2710106/1‑100 (MQ=255)
                                                           tGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGttt                                     <  1:2938892/100‑1 (MQ=255)
                                                             gAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCt                                   <  2:60313/100‑1 (MQ=255)
                                                              aaGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                  >  2:1859223/1‑100 (MQ=255)
                                                               aGCGCCCGCCTGGTGACTAAAACTCCGAAGTGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTa                                 <  2:3028544/100‑1 (MQ=255)
                                                                  gcccgccTGGTGACTAAAACTCCGAAGGGATCACCCACGGTAGTGTGCGAACGCCCTGTTGGCTTCGGCCATACGGTGACCGTCTTCACGTCTCTTAACt                              >  2:2131784/1‑100 (MQ=255)
                                                                   cccgctTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTg                             >  1:708261/1‑100 (MQ=255)
                                                                   cccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTg                             <  2:1685917/100‑1 (MQ=255)
                                                                    ccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGc                            <  1:3683034/100‑1 (MQ=255)
                                                                    ccgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGc                            >  2:1336474/1‑100 (MQ=255)
                                                                    ccgccTGGCGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGc                            <  1:2147161/100‑1 (MQ=255)
                                                                     cgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa                           <  2:2299005/100‑1 (MQ=255)
                                                                     cgccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCa                           <  1:3414212/100‑1 (MQ=255)
                                                                      gccTGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAg                          <  2:1910260/100‑1 (MQ=255)
                                                                         tGGTGACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAc                       >  1:2789452/1‑100 (MQ=255)
                                                                             gACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                   <  2:722682/100‑1 (MQ=255)
                                                                             gACTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCttt                   >  1:3123106/1‑100 (MQ=255)
                                                                              aCTAAAACGCCGAAGGGATCACCAACGTTAGTGTGCGAACGCCTTGTTGGCTTCGTCCATACTGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTg                  <  2:2858330/100‑1 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                 >  1:803515/1‑100 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                 >  2:1301112/1‑100 (MQ=255)
                                                                               cTAAAACTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGt                 >  1:3712008/1‑100 (MQ=255)
                                                                                 aaaaCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGttt               >  1:387496/1‑100 (MQ=255)
                                                                                  aaaCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGtttt              >  1:167221/1‑100 (MQ=255)
                                                                                    aCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt            >  2:221365/1‑100 (MQ=255)
                                                                                    aCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt            <  2:1088406/100‑1 (MQ=255)
                                                                                    aCTCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt            >  1:1224415/1‑100 (MQ=255)
                                                                                      tCCGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgc          >  1:2646592/1‑100 (MQ=255)
                                                                                      tCCGAACGGACCCCCAACGCTAGTGTGCGAACGCGTTCTTTGCCTCGGCCATACGGTGAGCGCCTTCTCGTTCCTTAACTGCAGTACCTTTGTTTTCttc          <  2:366285/100‑3 (MQ=255)
                                                                                       ccGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgta         >  1:445944/1‑98 (MQ=255)
                                                                                       ccGAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgca         <  2:3678104/100‑1 (MQ=255)
                                                                                         gAAGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagc       >  2:2007881/1‑100 (MQ=255)
                                                                                           aGGGATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTACGGCCAGACGGTGTACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagcan     <  1:766222/100‑2 (MQ=255)
                                                                                            gggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc    >  2:3136030/1‑100 (MQ=255)
                                                                                             ggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCa   >  1:688442/1‑100 (MQ=255)
                                                                                             ggATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCa   >  2:899978/1‑100 (MQ=255)
                                                                                              gATCACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGCTGAACGTCTTCACGTTTCTTCCCTGAAGTACCTTTGTTTTCTGCAGCATCAg  >  2:2542686/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAG  >  NC_000913/3473515‑3473708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: