Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,515,115 A→C L267V (TTG→GTG)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,515,1150AC99.4% 565.3 / ‑4.1 159L267V (TTG→GTG) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (73/85);  total (73/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 166 total aligned reads displayed.

TCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACTC  >  NC_000913/3515019‑3515211
                                                                                                |                                                                                                
 cAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGcc                                                                                              <  2:2674903/100‑1 (MQ=255)
  aGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTCTCCGCGGGTGAAACGACTCCTGGTTTGTACACAGCca                                                                                             <  2:3901002/100‑1 (MQ=255)
   gCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcag                                                                                            <  1:1522295/100‑1 (MQ=255)
    ccGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAcagccagc                                                                                           <  1:2779831/100‑1 (MQ=255)
    ccGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAcagccagc                                                                                           >  2:2146496/1‑100 (MQ=255)
    ccGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAcagccagc                                                                                           >  2:3241870/1‑100 (MQ=255)
     cGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagca                                                                                          >  2:306534/1‑100 (MQ=255)
     cGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagca                                                                                          >  2:1431479/1‑100 (MQ=255)
      gACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagcag                                                                                         <  1:1269083/100‑1 (MQ=255)
      gACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagcag                                                                                         <  2:877901/100‑1 (MQ=255)
      gACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCcagcag                                                                                         >  1:240651/1‑100 (MQ=255)
       aCAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGt                                                                                        <  1:76900/100‑1 (MQ=255)
        cAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGtt                                                                                       <  1:1998466/100‑1 (MQ=255)
          gcaTTGAGGGGGCAATCAAATACTGTTTCTTCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCg                                                                                     <  1:3726018/98‑1 (MQ=255)
          gAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCg                                                                                     >  1:1339344/1‑100 (MQ=255)
            aTTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTc                                                                                   <  1:3983568/100‑1 (MQ=255)
             ttGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCTTTGAGAATTATTTTTTCGCGGGTGCAACGACTCCTGCTTTGTACACAGCCAGCAgtccgtcc                                                                                  <  2:616712/100‑1 (MQ=255)
               gAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCAc                                                                                >  1:1152361/1‑100 (MQ=255)
                aGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCAcc                                                                               <  2:903880/100‑1 (MQ=255)
                aGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCAcc                                                                               <  2:3238149/100‑1 (MQ=255)
                 gggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCt                                                                              >  2:1576364/1‑100 (MQ=255)
                 gggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCt                                                                              >  1:1360333/1‑100 (MQ=255)
                  ggggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCtt                                                                             >  2:2290189/1‑100 (MQ=255)
                    ggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCtttt                                                                           <  2:2325949/100‑1 (MQ=255)
                    ggCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCtttt                                                                           <  1:973247/100‑1 (MQ=255)
                     gCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCttttt                                                                          <  2:1740137/100‑1 (MQ=255)
                     gCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCttttt                                                                          <  1:2917346/100‑1 (MQ=255)
                      cAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTa                                                                         <  1:2680334/100‑1 (MQ=255)
                       aaTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTAc                                                                        <  2:1412884/100‑1 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       >  2:1641333/1‑100 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       <  1:2210870/100‑1 (MQ=255)
                        aTCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACg                                                                       >  2:2516459/1‑100 (MQ=255)
                         tCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgt                                                                      >  2:3808123/1‑100 (MQ=255)
                          cAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtg                                                                     <  1:2776461/100‑1 (MQ=255)
                           aaaTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACgtgt                                                                    >  1:576753/1‑100 (MQ=255)
                            aaTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGtgtg                                                                   <  1:2775650/100‑1 (MQ=255)
                             aTACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTgc                                                                  <  1:808487/100‑1 (MQ=255)
                                 tGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTgccgcc                                                              <  1:2843582/100‑1 (MQ=255)
                                    ttCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtn                                                           <  1:523979/100‑2 (MQ=255)
                                    ttCATCCGCTTCTCCTGGAGAATTATTTTTTTTTTTTTTTTTCAACTCCTGGCGTGTACACAGCCAGCAGTTTGTCCTCCTTTTTACGTGTGCCGCCGtt                                                           >  2:3877713/1‑100 (MQ=255)
                                       aTCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTtgct                                                        <  1:649764/100‑1 (MQ=255)
                                          cGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTgctgct                                                     <  1:2866643/100‑1 (MQ=255)
                                          cGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTgctgct                                                     <  1:3013774/100‑1 (MQ=255)
                                          cGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTgctgct                                                     <  2:2889184/100‑1 (MQ=255)
                                          cGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTgctgct                                                     <  2:3928662/100‑1 (MQ=255)
                                           gCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCtt                                                    >  2:3456138/1‑100 (MQ=255)
                                               ctcCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATAc                                                <  2:3545593/100‑1 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              <  2:125424/100‑1 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              <  1:2180012/100‑1 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              >  1:1774314/1‑100 (MQ=255)
                                                 ccTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTg                                              <  1:1724858/100‑1 (MQ=255)
                                                   ttGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCg                                            >  1:2762238/1‑100 (MQ=255)
                                                   ttGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCg                                            >  1:2295515/1‑100 (MQ=255)
                                                     gagaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTc                                          <  1:3434011/100‑1 (MQ=255)
                                                     gagaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTc                                          <  1:3216103/100‑1 (MQ=255)
                                                     gagaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTc                                          <  1:2449232/100‑1 (MQ=255)
                                                      agaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCTGCCGTTGCTGCTCATACTGCGTCg                                         >  2:484434/1‑100 (MQ=255)
                                                       gaATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGa                                        >  1:2559603/1‑100 (MQ=255)
                                                         attattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACTTGTGCCGCCGTTGCTGCTTATACTGCGTCGAAc                                      >  2:510453/1‑100 (MQ=255)
                                                         attattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAAc                                      <  1:1740305/100‑1 (MQ=255)
                                                         attattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAAc                                      <  1:1125317/100‑1 (MQ=255)
                                                         attattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAAc                                      >  2:1142610/1‑100 (MQ=255)
                                                         attattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAAc                                      <  2:1107397/100‑1 (MQ=255)
                                                          ttattTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACt                                     <  2:2201221/100‑1 (MQ=255)
                                                            attTTTTCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACttt                                   >  1:1534909/1‑100 (MQ=255)
                                                             ttttttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTg                                  >  1:3819086/1‑100 (MQ=255)
                                                             ttttttCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTg                                  <  1:306327/100‑1 (MQ=255)
                                                                tttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacg                               <  1:171893/100‑1 (MQ=255)
                                                                tttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacg                               <  2:1704258/100‑1 (MQ=255)
                                                                tttCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacg                               >  1:1865264/1‑100 (MQ=255)
                                                                  tCGCGGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTgacgac                             >  1:3016059/1‑100 (MQ=255)
                                                                   cgcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACa                            <  1:2497046/100‑1 (MQ=255)
                                                                   cgcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACGTTGACGACa                            <  1:1456933/100‑1 (MQ=255)
                                                                    gcgGGTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACAt                           <  2:2074460/100‑1 (MQ=255)
                                                                       ggTGAAACGGCTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTCTTACGTGTGCCGTCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCa                        >  1:3983413/1‑100 (MQ=255)
                                                                       ggTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCa                        <  1:1818740/100‑1 (MQ=255)
                                                                       ggTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCa                        >  1:1600816/1‑100 (MQ=255)
                                                                       ggTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCa                        >  2:3583928/1‑100 (MQ=255)
                                                                       ggTGAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCa                        >  2:1582832/1‑100 (MQ=255)
                                                                          gAAACGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAtt                     >  1:1084794/1‑100 (MQ=255)
                                                                           taaCGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAttt                    <  2:1452747/99‑1 (MQ=255)
                                                                            aaCGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAAtttt                   >  1:1893381/1‑100 (MQ=255)
                                                                             aCGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTg                  <  1:713256/100‑1 (MQ=255)
                                                                              cGACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgc                 <  1:2075651/100‑1 (MQ=255)
                                                                               gACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcg                <  1:2175823/100‑1 (MQ=255)
                                                                               gACTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcg                <  1:3707158/100‑1 (MQ=255)
                                                                                 cTCCTGGTTTGTACCCAGCCAGCAGTTCGTCCACCTTTTTACGTTTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgcg              <  2:2639351/100‑1 (MQ=255)
                                                                                 cTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgcg              >  1:244047/1‑100 (MQ=255)
                                                                                 cTCCTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTgcgcg              >  2:69121/1‑100 (MQ=255)
                                                                                   ccTGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCt            >  1:919978/1‑100 (MQ=255)
                                                                                     tGGTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGa          >  2:3563545/1‑100 (MQ=255)
                                                                                      ggTTTGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGAt         <  1:869546/100‑1 (MQ=255)
                                                                                        tttGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATAc       <  1:2325705/100‑1 (MQ=255)
                                                                                        tttGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTAGCGCGCTGATAc       <  2:887124/100‑1 (MQ=255)
                                                                                         ttGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATAcc      <  1:312001/100‑1 (MQ=255)
                                                                                         ttGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATAcc      <  2:2866980/100‑1 (MQ=255)
                                                                                         ttGTACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATAcc      <  1:1165245/100‑1 (MQ=255)
                                                                                         ttGTACACAGCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCa     <  2:211983/100‑1 (MQ=255)
                                                                                           gTACACAGCCAGCATTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCAAACTTTGACGACATGCACTTTTTAGCGCTGATACCaa    >  2:3725095/1‑99 (MQ=255)
                                                                                            tACACAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACt   >  2:1994219/1‑100 (MQ=255)
                                                                                                |                                                                                                
TCAGCCGACAGAATTGAGGGGGCAATCAAATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTATACTGCGTCGAACTTTGACGACATGCAATTTTGCGCGCTGATACCACTC  >  NC_000913/3515019‑3515211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: