Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,520,438 A→C L89R (CTC→CGC)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,520,4380AC98.7% 517.3 / ‑2.4 150L89R (CTC→CGC) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (75/73);  total (76/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 152 total aligned reads displayed.

ACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCG  >  NC_000913/3520341‑3520534
                                                                                                 |                                                                                                
aCTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTgagcga                                                                                                >  2:2939007/1‑100 (MQ=255)
 cTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGaa                                                                                               >  1:1172011/1‑100 (MQ=255)
 cTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGaa                                                                                               >  1:75892/1‑100 (MQ=255)
 cTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGaa                                                                                               >  1:3988852/1‑100 (MQ=255)
   gCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATg                                                                                             >  2:251164/1‑100 (MQ=255)
      gttctCCAGCAGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTg                                                                                          <  2:3479082/96‑1 (MQ=255)
       ttttCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGc                                                                                         <  1:1141384/100‑1 (MQ=255)
        tttCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGcc                                                                                        >  1:867175/1‑100 (MQ=255)
        tttCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGcc                                                                                        >  1:2603344/1‑100 (MQ=255)
         ttCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCAATATTGTTATTCTGCCAAGCAATGGAATGCACTGACCGAATGTTGCCt                                                                                       >  1:3885829/1‑100 (MQ=255)
            cAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTcc                                                                                    <  2:418214/100‑1 (MQ=255)
            cAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTcc                                                                                    >  1:1914348/1‑100 (MQ=255)
            cAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAAAGTTGCCTTcc                                                                                    >  2:2916144/1‑100 (MQ=255)
             agcagcAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCt                                                                                   >  1:3144294/1‑100 (MQ=255)
             aGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCt                                                                                   <  2:2719681/100‑1 (MQ=255)
             aGCCGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCt                                                                                   >  1:3144330/1‑100 (MQ=255)
              gCGGCAGATTTGCCTGCGTCCGCGCCTGCTCCGACTCCTGGCGGGCGATATTGTTATTCTGCCAGGAAATGGAATGCGCTGAGCCAATGTTGCCTTCCTg                                                                                  <  2:1622714/100‑1 (MQ=255)
              gCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTg                                                                                  >  1:1261317/1‑100 (MQ=255)
               cGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGc                                                                                 >  2:2432535/1‑100 (MQ=255)
                ggCAGATTTGCCTGCGCCTGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGcc                                                                                >  2:1325221/1‑100 (MQ=255)
                ggCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGcc                                                                                >  2:1573731/1‑100 (MQ=255)
                  cAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCcgc                                                                              >  1:2721699/1‑100 (MQ=255)
                   aGATTTGCCTGCGCCCGCGCCTGCTTCGCCTCCTGGCTGGCGATATCGTTATTCTGCCAGGCAATTGAATGCACTGAGCGAATGTTGCCTTCCTGCcgcg                                                                             <  1:3796341/100‑1 (MQ=255)
                   aGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCcgcg                                                                             <  1:261519/100‑1 (MQ=255)
                    gATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGt                                                                            >  2:1146748/1‑100 (MQ=255)
                    gATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGt                                                                            <  2:1729835/100‑1 (MQ=255)
                     aTTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGCATGCACTGAGCGCATGTTGCCTTCCTCCCGCGtt                                                                           >  1:3327900/1‑100 (MQ=255)
                     aTTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGtt                                                                           >  1:2282624/1‑100 (MQ=255)
                     aTTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGtt                                                                           >  2:2080237/1‑100 (MQ=255)
                        tGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATc                                                                        <  2:1543925/100‑1 (MQ=255)
                        tGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATc                                                                        <  1:1024298/100‑1 (MQ=255)
                        tGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATc                                                                        <  1:1506739/100‑1 (MQ=255)
                         gCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCa                                                                       <  2:3917512/100‑1 (MQ=255)
                         gCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATACTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTACCGCGTTATCa                                                                       >  2:3075422/1‑100 (MQ=255)
                          ccTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATcgg                                                                      >  1:943086/1‑98 (MQ=255)
                          ccTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAg                                                                      >  1:1805396/1‑100 (MQ=255)
                           cTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGt                                                                     >  1:195863/1‑100 (MQ=255)
                           cTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGt                                                                     >  2:1028977/1‑100 (MQ=255)
                           cTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGt                                                                     >  1:3989657/1‑100 (MQ=255)
                             gcgcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTcc                                                                   <  1:1197586/100‑1 (MQ=255)
                               gcCCGCGCCTGCTCCGCCTCCTGTCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCgg                                                                 <  2:709839/100‑1 (MQ=255)
                               gcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCgg                                                                 >  1:1021210/1‑100 (MQ=255)
                               gcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCgg                                                                 <  2:1400817/100‑1 (MQ=255)
                                cccGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgc                                                                <  1:584079/100‑1 (MQ=255)
                                cccGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGCATGTTGCCTTCCTGCCGCGTTATCAGTCCGgc                                                                >  2:370203/1‑100 (MQ=255)
                                cccGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTACACGCATGTTGCCTTCCTGCCACGTTATCACTCCGgc                                                                >  1:4077922/1‑100 (MQ=255)
                                  cgcgCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcgc                                                              >  1:3070005/1‑100 (MQ=255)
                                  cgcgCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGgcgc                                                              <  2:3906135/100‑1 (MQ=255)
                                   gcgcCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCt                                                             <  1:1774121/100‑1 (MQ=255)
                                     gcCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCttt                                                           >  1:2046624/1‑100 (MQ=255)
                                       cTGCTCCGCCTCCTGGCGGGAGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTc                                                         <  2:1266692/100‑1 (MQ=255)
                                         gCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCAc                                                       <  2:2232076/100‑1 (MQ=255)
                                              gCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAg                                                  >  1:2334990/1‑100 (MQ=255)
                                               cctcctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGt                                                 <  2:2536590/100‑1 (MQ=255)
                                                ctcctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGtt                                                <  1:4073324/100‑1 (MQ=255)
                                                   ggcgcgggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGt                                             <  1:3600848/97‑1 (MQ=255)
                                                   ctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGt                                             >  1:3488278/1‑100 (MQ=255)
                                                    tGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTa                                            <  1:1687573/100‑1 (MQ=255)
                                                    tGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTa                                            <  1:3403256/100‑1 (MQ=255)
                                                     ggcgggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAg                                           >  2:1886011/1‑100 (MQ=255)
                                                     ggcgggcgAGAGGGTGATGCCGCCAGGCAATGGAATGCACGGTCCGAATGTTTCCTTCCTGCCGCGTGAGTAGGCCGGCGCTTTTCACTAAAGTTTGTAg                                           <  2:689563/100‑1 (MQ=255)
                                                      gcgggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGt                                          >  1:2600561/1‑100 (MQ=255)
                                                      gcgggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGt                                          <  2:1455636/100‑1 (MQ=255)
                                                       cgggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTg                                         <  2:889946/100‑1 (MQ=255)
                                                        gggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGc                                        <  2:3457784/100‑1 (MQ=255)
                                                         ggcgATTTTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGcc                                       <  2:105789/100‑1 (MQ=255)
                                                         ggcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGcc                                       <  1:600340/100‑1 (MQ=255)
                                                          gcgATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCt                                      >  2:3959509/1‑100 (MQ=255)
                                                             atatTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCt                                   <  1:1146375/100‑1 (MQ=255)
                                                              tatTGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCtt                                  >  1:494488/1‑100 (MQ=255)
                                                                ttGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTcc                                >  1:2856726/1‑100 (MQ=255)
                                                                 tGTTATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCa                               <  2:1755422/100‑1 (MQ=255)
                                                                   ttATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAgg                             <  2:1847119/100‑1 (MQ=255)
                                                                   ttATTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAgg                             <  2:646056/100‑1 (MQ=255)
                                                                    tATTCTGCCAGGCAATGGAATGCACCGAGCGAATGTTGCCTTCCTGCCGCGTTATCCGTCCGGGTCTTTTCACTACAGCTTGTTGTGCCTGCTTCCCggg                            >  2:2722777/1‑100 (MQ=255)
                                                                     aTTGTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGa                           >  2:3668243/1‑100 (MQ=255)
                                                                     aTTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGa                           <  2:1645182/100‑1 (MQ=255)
                                                                     aTTCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGa                           <  1:3041805/100‑1 (MQ=255)
                                                                      ttCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGaa                          <  1:1271011/100‑1 (MQ=255)
                                                                      ttCTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGaa                          >  2:2069751/1‑100 (MQ=255)
                                                                        cTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACa                        >  1:3855551/1‑100 (MQ=255)
                                                                        cTGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACa                        >  2:2371366/1‑100 (MQ=255)
                                                                         tGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                       >  1:680683/1‑100 (MQ=255)
                                                                         tGCCAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                       >  2:1315780/1‑100 (MQ=255)
                                                                           ccAGGCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCt                     >  1:520627/1‑100 (MQ=255)
                                                                              ggCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGGCTGCTTCCAGGGAACATCTGtt                  <  1:3416631/100‑1 (MQ=255)
                                                                              ggCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGtt                  <  2:565194/100‑1 (MQ=255)
                                                                               gCAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTa                 <  2:1125115/100‑1 (MQ=255)
                                                                                cAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAg                >  2:3268676/1‑100 (MQ=255)
                                                                                cAATGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAg                <  2:2014637/100‑1 (MQ=255)
                                                                                  aTGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGAt              >  1:2204521/1‑100 (MQ=255)
                                                                                  aTGGAATGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGAt              <  1:2082841/100‑1 (MQ=255)
                                                                                       gtGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCCCTACAGTTTGTAGTGCCTGCTTCCAGGGAACCTCTGTTAGATGTAAc         <  2:3044108/99‑1 (MQ=255)
                                                                                       aTGCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAAc         <  1:1848308/100‑1 (MQ=255)
                                                                                         gCACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGa       <  1:685714/100‑1 (MQ=255)
                                                                                          cACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCTGCGCTTTTCAATACAGTTTGTAACGCCTGCTACCAGGGAACAGCTGTTAGATATCACGac      >  2:3267349/1‑100 (MQ=255)
                                                                                          cACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGac      >  1:805343/1‑100 (MQ=255)
                                                                                          cACTGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGac      >  2:2457736/1‑100 (MQ=255)
                                                                                             tGAGCGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACAcc   <  1:726664/100‑1 (MQ=255)
                                                                                              gagcgaATGTTGCCTTCCTGCGGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCATTGAACATCTGTTAGATGTAACGACACCg  <  2:1340486/100‑1 (MQ=255)
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ACTGCGATTTTCCAGCGGCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCG  >  NC_000913/3520341‑3520534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑