Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,602,989 T→G I419L (ATT→CTT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,602,9890TG98.7% 544.8 / NA 156I419L (ATT→CTT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (75/78);  total (77/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

GTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCAG  >  NC_000913/3602892‑3603085
                                                                                                 |                                                                                                
gtgccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAagag                                                                                                <  1:3637899/100‑1 (MQ=255)
  gccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTc                                                                                              >  1:881086/1‑100 (MQ=255)
   ccgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCa                                                                                             <  1:1368975/100‑1 (MQ=255)
    cgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAg                                                                                            >  1:75454/1‑100 (MQ=255)
    cgtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAg                                                                                            >  1:3208101/1‑100 (MQ=255)
     gtCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGc                                                                                           >  1:2289285/1‑100 (MQ=255)
      tCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCa                                                                                          <  1:3148397/100‑1 (MQ=255)
       ccAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCAt                                                                                         >  1:2046403/1‑100 (MQ=255)
        cAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATa                                                                                        <  2:1594618/100‑1 (MQ=255)
        cAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATa                                                                                        >  1:3310959/1‑100 (MQ=255)
        cAGTTTCGTTAGCGTGATGCCAGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATa                                                                                        <  1:3802615/100‑1 (MQ=255)
          gTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAAc                                                                                      >  1:281562/1‑100 (MQ=255)
          gTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAAc                                                                                      <  1:1908129/100‑1 (MQ=255)
              cGTTAGCGTGATGCTGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATGAAGAGTCAGCATAACTTCa                                                                                  <  2:3657563/100‑1 (MQ=255)
               gTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCAt                                                                                 >  1:103564/1‑100 (MQ=255)
               gTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCAt                                                                                 >  1:1404420/1‑100 (MQ=255)
                    cGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATgcggc                                                                            >  1:2975465/1‑100 (MQ=255)
                     gTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATgcggcg                                                                           >  1:3088082/1‑100 (MQ=255)
                      tGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGgcgc                                                                          <  1:1508194/100‑1 (MQ=255)
                      tGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGgcgc                                                                          >  1:285549/1‑100 (MQ=255)
                           ccGGTTAAGCCAACGGCTTTATGGAACAGTTTGCCCGGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCGGCATAACGTCATGCGGCGCTGCaa                                                                     >  2:709788/1‑100 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACg                                                                   >  2:1349087/1‑100 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACg                                                                   <  1:3392540/100‑1 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACg                                                                   <  1:1472724/100‑1 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATACGGCGCTTCAACg                                                                   >  1:3680948/1‑100 (MQ=255)
                             ggTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCGTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACg                                                                   <  1:2394401/100‑1 (MQ=255)
                              gTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGt                                                                  >  1:529111/1‑100 (MQ=255)
                               ttAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                                                                 >  2:3001152/1‑100 (MQ=255)
                                tAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCg                                                                <  1:2788415/100‑1 (MQ=255)
                                  aGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAg                                                              <  1:2854354/100‑1 (MQ=255)
                                   gCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGt                                                             >  2:1700857/1‑100 (MQ=255)
                                   gCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGt                                                             >  2:1216470/1‑100 (MQ=255)
                                   gCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGt                                                             >  1:1639341/1‑100 (MQ=255)
                                    ccAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGtt                                                            <  1:1404264/100‑1 (MQ=255)
                                       aCGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                                                         <  2:1992118/100‑1 (MQ=255)
                                        cGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                                                        >  1:2826537/1‑100 (MQ=255)
                                        cGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                                                        <  1:3639632/100‑1 (MQ=255)
                                         ggCTTCATGGAACAGTTTGGCCTAGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGGGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                                                       <  2:1909585/100‑1 (MQ=255)
                                          acTTCATGGAACAGTTTGGCCTGGCTTACCGCGTGCTGGCCGGTGCTGGCATTAATAGTCAGCATAACTTCATGTTGCGCTTCAACGTGGTGTTTCTtca                                                      >  2:1385935/2‑100 (MQ=255)
                                           cTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcat                                                     >  1:3454299/1‑100 (MQ=255)
                                           cTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcat                                                     <  2:1477806/100‑1 (MQ=255)
                                            ttCCTGGCACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatn                                                    <  1:3263913/100‑2 (MQ=255)
                                            ttCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatc                                                    >  2:2434051/1‑100 (MQ=255)
                                            ttCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatc                                                    >  2:2665371/1‑100 (MQ=255)
                                               aTGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcg                                                 >  2:2552649/1‑100 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                >  1:123388/1‑100 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                >  1:274026/1‑100 (MQ=255)
                                                tGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc                                                >  1:1368917/1‑100 (MQ=255)
                                                 ggAACAGTTTGGCCTTGCTTACCGCGTGCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTTATGCGGGGCGTCAACGTCGAGGTTCTTCATCAcgcc                                               >  2:864271/1‑99 (MQ=255)
                                                 ggAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgcg                                               >  2:75169/1‑100 (MQ=255)
                                                 ggAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgcg                                               <  2:2622650/100‑1 (MQ=255)
                                                  gAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg                                              >  1:2584108/1‑100 (MQ=255)
                                                    aCAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGAc                                            <  2:2930413/100‑1 (MQ=255)
                                                     cAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACg                                           >  1:1033197/1‑100 (MQ=255)
                                                      aGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGa                                          >  1:2600568/1‑100 (MQ=255)
                                                         ttcgCCTTGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCAAGTTTCTTCATCACGCGGACGAttt                                       <  2:1840605/97‑1 (MQ=255)
                                                          tGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtttt                                      <  2:3857500/100‑1 (MQ=255)
                                                           ggCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttc                                     <  2:967719/100‑1 (MQ=255)
                                                            gCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct                                    <  2:1675921/100‑1 (MQ=255)
                                                            gCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct                                    <  2:3975150/100‑1 (MQ=255)
                                                            gCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct                                    <  2:1373189/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  2:1177233/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  2:3092243/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  2:3146667/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  1:1442087/100‑1 (MQ=255)
                                                              cTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttcttc                                  <  1:1993147/100‑1 (MQ=255)
                                                               tGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCa                                 >  2:3809084/1‑100 (MQ=255)
                                                                 gCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAc                               <  1:1913596/100‑1 (MQ=255)
                                                                 gCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAc                               <  2:518716/100‑1 (MQ=255)
                                                                 gCTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCAGGCGGCGTTTCAACGTCGAGTTTCTGCATCACGCGGACGATTTTCTTCAAc                               <  2:739272/100‑1 (MQ=255)
                                                                  cTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAct                              >  1:3948087/1‑100 (MQ=255)
                                                                  cTTACCGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAAct                              <  2:1011626/100‑1 (MQ=255)
                                                                      ccGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTc                          >  2:3666599/1‑100 (MQ=255)
                                                                      ccGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTc                          <  1:661575/100‑1 (MQ=255)
                                                                      ccGCGTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTc                          <  1:1566209/100‑1 (MQ=255)
                                                                         cgTTCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATTCGGCGCTTCCACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCAt                       >  2:654620/1‑100 (MQ=255)
                                                                           ttCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCa                     <  1:3746079/100‑1 (MQ=255)
                                                                           ttCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCa                     <  1:1247059/100‑1 (MQ=255)
                                                                           ttCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCa                     <  1:490467/100‑1 (MQ=255)
                                                                            tCTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAg                    <  1:2160036/100‑1 (MQ=255)
                                                                             cTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAgg                   <  2:862460/100‑1 (MQ=255)
                                                                             cTGCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAgg                   <  2:3570447/100‑1 (MQ=255)
                                                                               gCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTg                 >  2:1468150/1‑100 (MQ=255)
                                                                               gCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTg                 >  2:239912/1‑100 (MQ=255)
                                                                               gCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTg                 >  1:3703235/1‑100 (MQ=255)
                                                                               gCCCGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTg                 >  1:2426285/1‑100 (MQ=255)
                                                                                cccGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGc                >  2:1287444/1‑100 (MQ=255)
                                                                                 ccGGTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCg               >  2:1495811/1‑100 (MQ=255)
                                                                                  cGGTGCTAGCATCAAGAGTCAGCATAACCTCATGCTGCGCTTGAACGTCGACTTTCTTCATCCAGCGTAAGATTTTCTTCAAATCTTCCATCAGGTGCGa              <  2:1090911/100‑1 (MQ=255)
                                                                                   ggTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGAt             <  2:2461557/100‑1 (MQ=255)
                                                                                    gTGCTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGAtt            <  1:3521167/100‑1 (MQ=255)
                                                                                      gCTGGCATCAAGGGTCAGCATAACTCCATGCGGCGCTTCAACGCCGAGGTTCTTCATCACGCAGACGATATTCTTCCACTCTTCCACCACGAGCGCTTTg          >  2:2217116/1‑100 (MQ=255)
                                                                                       cTGGCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGt         >  2:445869/1‑100 (MQ=255)
                                                                                         ngCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTc       >  1:992016/2‑100 (MQ=255)
                                                                                         ggCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTc       <  2:3306477/100‑1 (MQ=255)
                                                                                         ggCATCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTc       <  1:98456/100‑1 (MQ=255)
                                                                                           cATCAAGAGACAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTg     >  2:2041997/1‑100 (MQ=255)
                                                                                            aTCAAGAGTCAGCATAACTTCAGGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGc    <  2:3344317/100‑1 (MQ=255)
                                                                                             tCAAGAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCa   >  1:3305498/1‑100 (MQ=255)
                                                                                              cAAGAGTCAGCATAACTTCATGCGGCGCTTCAAAGTCGAGCTTGTTCATCACGCGGGAGACTGTCTTCAACTCTTCCATCCGGCGCGAGTTGTTCtcccc  >  2:2395805/1‑96 (MQ=255)
                                                                                                 |                                                                                                
GTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTTCAACTCTTCCATCAGGTGCGATTTGTTCTGCAG  >  NC_000913/3602892‑3603085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: