Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 301,463 T→G E142A (GAA→GCA)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913301,4630TG98.4% 629.8 / 0.7 182E142A (GAA→GCA) paoBPaoABC aldehyde oxidoreductase, FAD‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (94/85);  total (96/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

Only 100 of 182 total aligned reads displayed.

CGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTG  >  NC_000913/301366‑301559
                                                                                                 |                                                                                                
cGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCa                                                                                                <  2:1747620/100‑1 (MQ=255)
    tCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcg                                                                                            >  1:4047550/1‑100 (MQ=255)
     ccAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcgc                                                                                           >  2:285561/1‑100 (MQ=255)
     ccAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAgcgc                                                                                           <  2:3610683/100‑1 (MQ=255)
      cAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGcgcc                                                                                          <  1:1058641/100‑1 (MQ=255)
      cAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGcgcc                                                                                          >  1:3962361/1‑100 (MQ=255)
       aGCAACCGCATTGCGACCGCCATAGCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAGGCCTGCAAGcgccg                                                                                         <  2:784837/100‑1 (MQ=255)
           aCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCa                                                                                     <  2:2682724/100‑1 (MQ=255)
            gcGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTGACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAg                                                                                    <  2:971932/99‑1 (MQ=255)
             cGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGc                                                                                   <  2:1426131/100‑1 (MQ=255)
             cGCATTGCGACCGCCATATCGCTCGGATGGGTGCCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGc                                                                                   <  2:2374769/100‑1 (MQ=255)
              gCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGcc                                                                                  <  1:1951214/100‑1 (MQ=255)
               cATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCg                                                                                 <  1:2414985/100‑1 (MQ=255)
               cATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCg                                                                                 <  1:2053294/100‑1 (MQ=255)
               cATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCg                                                                                 <  2:1673384/100‑1 (MQ=255)
                   gCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTcc                                                                             <  1:3674130/100‑1 (MQ=255)
                    cGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTccc                                                                            >  1:2337361/1‑100 (MQ=255)
                     gACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCg                                                                           <  1:2053577/100‑1 (MQ=255)
                        cGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGc                                                                        <  2:1945747/100‑1 (MQ=255)
                        cGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGc                                                                        <  2:2217102/100‑1 (MQ=255)
                         gCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCa                                                                       >  1:3414043/1‑100 (MQ=255)
                         gCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCa                                                                       >  1:3751052/1‑100 (MQ=255)
                          ccATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAg                                                                      <  1:496974/100‑1 (MQ=255)
                          ccATATCGATCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTACCGGGCAg                                                                      >  1:1264472/1‑100 (MQ=255)
                            atatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgc                                                                    <  1:1700374/100‑1 (MQ=255)
                            atatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgc                                                                    <  1:2592667/100‑1 (MQ=255)
                             tatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgcg                                                                   <  2:447875/100‑1 (MQ=255)
                             tatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgcg                                                                   <  2:1563541/100‑1 (MQ=255)
                             tatCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgcg                                                                   >  1:349994/1‑100 (MQ=255)
                              atCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGgcgc                                                                  >  2:3282026/1‑100 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 <  2:2437652/100‑1 (MQ=255)
                               tCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCt                                                                 <  1:750105/100‑1 (MQ=255)
                                cGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCtt                                                                >  2:1224333/1‑100 (MQ=255)
                                 gCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTa                                                               <  1:2913307/100‑1 (MQ=255)
                                  ctctGATGGGTGGCAATGCGGGCTTCGCTTACGCCTACCACCGCGTGCTGCCGCCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTAt                                                              <  2:430651/100‑1 (MQ=255)
                                  cTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTAt                                                              <  2:3356431/100‑1 (MQ=255)
                                  cTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTAt                                                              >  1:1286491/1‑100 (MQ=255)
                                     ggATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGc                                                           >  2:3558516/1‑100 (MQ=255)
                                     ggATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGc                                                           <  1:3629098/100‑1 (MQ=255)
                                       aTGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAg                                                         <  2:2794466/100‑1 (MQ=255)
                                        tGGGTGGCAATGCAGGCTTTGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAgg                                                        >  2:259159/1‑100 (MQ=255)
                                        tGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAgg                                                        <  1:1667954/100‑1 (MQ=255)
                                        tGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAgg                                                        <  2:715540/100‑1 (MQ=255)
                                         gggTGGCAATGCAGGCTTCGCTGACGCCTGCCGCCGCGTGCTGGCGGCTAAAGCCGGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAggn                                                       <  1:1780417/100‑2 (MQ=255)
                                          ggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCTGGCGCTTATTGCAggtc                                                      >  1:1621006/1‑98 (MQ=255)
                                          ggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGc                                                      >  1:4012925/1‑100 (MQ=255)
                                          ggTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGc                                                      >  1:2131208/1‑100 (MQ=255)
                                             ggCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCACCCGGGCAGGCGCTTATTGCTGGCCTGa                                                   >  2:598651/1‑100 (MQ=255)
                                             ggCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCGCAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTCTTGCAGGGCTGa                                                   >  2:3170625/1‑100 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 <  1:3532469/100‑1 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 <  2:2269967/100‑1 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 >  1:183932/1‑100 (MQ=255)
                                               cAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGAtt                                                 >  1:2202111/1‑100 (MQ=255)
                                                aaTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGGTTg                                                >  2:2524558/1‑100 (MQ=255)
                                                aaTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTg                                                >  1:965758/1‑100 (MQ=255)
                                                 aTGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTgg                                               >  1:1717416/1‑100 (MQ=255)
                                                  tGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGgt                                              >  2:3343395/1‑100 (MQ=255)
                                                   gCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGgtg                                             >  2:102972/1‑100 (MQ=255)
                                                     aGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTc                                           >  1:943049/1‑100 (MQ=255)
                                                      ggCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCg                                          <  1:3924985/100‑1 (MQ=255)
                                                       gCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCCGGCGCTTATTGCAGGGCTGATTGGTGTCGt                                         >  1:1407618/1‑100 (MQ=255)
                                                       gCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCGGGGCTGATTGGTGTCGt                                         >  1:190448/1‑100 (MQ=255)
                                                               tACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAg                                 >  1:1855190/1‑100 (MQ=255)
                                                               tACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAg                                 <  2:2128188/100‑1 (MQ=255)
                                                               tACGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAg                                 >  1:2419785/1‑100 (MQ=255)
                                                                aCGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgg                                <  1:1574989/100‑1 (MQ=255)
                                                                 cGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAggg                               >  2:1707444/1‑100 (MQ=255)
                                                                 cGCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAggg                               <  1:2634599/100‑1 (MQ=255)
                                                                  gCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgggg                              <  1:1784646/100‑1 (MQ=255)
                                                                  gCCTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGAAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAgggg                              <  2:3964093/100‑1 (MQ=255)
                                                                   ccTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGc                             >  2:3774345/1‑100 (MQ=255)
                                                                    cTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCa                            <  1:1530696/100‑1 (MQ=255)
                                                                    cTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGCCGTAAAAATAGGGGCa                            >  1:3356306/1‑100 (MQ=255)
                                                                    cTACCACCGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCCCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCa                            >  2:1122366/1‑100 (MQ=255)
                                                                      accaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgc                          <  2:3676245/100‑1 (MQ=255)
                                                                       ncaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         >  1:1174014/2‑100 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         >  2:918017/1‑100 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         <  1:2683520/100‑1 (MQ=255)
                                                                       ccaccGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAgcg                         >  1:2442600/1‑100 (MQ=255)
                                                                         accGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGcgcg                       <  1:3855327/100‑1 (MQ=255)
                                                                         accGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGcgcg                       <  1:3718041/100‑1 (MQ=255)
                                                                         accGCGTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGcgcg                       >  2:571189/1‑100 (MQ=255)
                                                                            gcgTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTgc                    <  1:3294513/100‑1 (MQ=255)
                                                                             cgTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTgcg                   <  2:2347559/100‑1 (MQ=255)
                                                                              ntGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTgcgc                  >  1:613993/2‑100 (MQ=255)
                                                                              gTGCTGACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTgcgc                  >  1:1464320/1‑100 (MQ=255)
                                                                                   gACGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGGTGATTGGTGTCGTAAAAATAGGGGCAGCGCACGCGCTgccc             <  2:3430318/100‑4 (MQ=255)
                                                                                    aCGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCACGCGCTgcccc            <  2:3671487/100‑5 (MQ=255)
                                                                                     cGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCa           <  1:193213/100‑1 (MQ=255)
                                                                                     cGGCTAAAGCCTGCAAGCGCCGCGCAGCCGCTACCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCACGCGCTgaccct           <  2:3391706/100‑6 (MQ=255)
                                                                                        cTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAt        >  1:1340016/1‑100 (MQ=255)
                                                                                        cTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAt        >  2:2564463/1‑100 (MQ=255)
                                                                                        cTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAt        >  2:2814071/1‑100 (MQ=255)
                                                                                        cTAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGAGTGCGATGGAGCAGAt        >  2:1997750/1‑100 (MQ=255)
                                                                                         tAAAGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGAtt       <  2:34929/100‑1 (MQ=255)
                                                                                          aaaGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTa      >  1:1988364/1‑100 (MQ=255)
                                                                                            aGCCTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTAcc    <  1:416132/100‑1 (MQ=255)
                                                                                              ccTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTaagat  >  1:1595427/1‑96 (MQ=255)
                                                                                              ccTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTg  >  1:257826/1‑100 (MQ=255)
                                                                                              ccTGCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTg  >  1:2673275/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCGCGCAGCCGCTCCCGGGCAGGCGCTTATTGCAGGGCTGATTGGTGTCGTAAAAATAGGGGCAGCGCGTGCGCTGGAGCAGATTACCTG  >  NC_000913/301366‑301559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑