Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 313,275 T→G intergenic (‑33/+441) ykgR ← / ← ykgP uncharacterized protein/pseudogene, oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913313,2750TG98.7% 515.1 / ‑2.4 149intergenic (‑33/+441)ykgR/ykgPuncharacterized protein/pseudogene, oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (66/80);  total (69/80)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 149 total aligned reads displayed.

ATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATT  >  NC_000913/313178‑313371
                                                                                                 |                                                                                                
aTTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCg                                                                                                <  2:758222/100‑1 (MQ=255)
  tGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTa                                                                                              >  1:1528920/1‑100 (MQ=255)
  tGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTa                                                                                              >  1:2471473/1‑100 (MQ=255)
   gCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTaa                                                                                             >  1:938718/1‑100 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            >  1:2520026/1‑100 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            <  2:673623/100‑1 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            <  2:129221/100‑1 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            >  1:2301082/1‑100 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            >  1:2301313/1‑100 (MQ=255)
        aaaaaCAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGcc                                                                                        <  1:2955315/100‑1 (MQ=255)
          aaaCAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCt                                                                                      <  1:790107/100‑1 (MQ=255)
           aacaacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCtt                                                                                     <  2:1656274/100‑1 (MQ=255)
             caacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtat                                                                                   >  1:329086/1‑100 (MQ=255)
              aacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtata                                                                                  >  1:3333266/1‑100 (MQ=255)
                     ttAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATataaataa                                                                           >  1:1257340/1‑100 (MQ=255)
                      tAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAAc                                                                          >  1:2636404/1‑100 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         <  2:821553/100‑1 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         <  2:3927112/100‑1 (MQ=255)
                        aTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGa                                                                        >  2:2313856/1‑100 (MQ=255)
                         tCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAg                                                                       >  2:2312960/1‑100 (MQ=255)
                            gTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGttt                                                                    >  1:3286549/1‑100 (MQ=255)
                            gTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGttt                                                                    <  2:640716/100‑1 (MQ=255)
                            gTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAACTGCCCTTATATAAATAACGAGttt                                                                    >  2:1440943/1‑100 (MQ=255)
                              ttATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTgg                                                                  <  1:559599/100‑1 (MQ=255)
                               tATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGt                                                                 <  1:1216714/100‑1 (MQ=255)
                                aTGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTc                                                                <  2:2495764/100‑1 (MQ=255)
                                 tGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCa                                                               >  1:2240026/1‑100 (MQ=255)
                                  gACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCaa                                                              >  1:416446/1‑100 (MQ=255)
                                    cTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAAcc                                                            <  2:2057910/100‑1 (MQ=255)
                                    cTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAAcc                                                            >  2:979016/1‑100 (MQ=255)
                                    cTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAAcc                                                            >  2:4002916/1‑100 (MQ=255)
                                     tGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCa                                                           >  2:2626048/1‑100 (MQ=255)
                                      gATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCaa                                                          >  1:38695/1‑100 (MQ=255)
                                      gATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCaa                                                          >  1:2825250/1‑100 (MQ=255)
                                         ttGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAttt                                                       <  2:1991952/100‑1 (MQ=255)
                                          tGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAtttt                                                      >  1:610357/1‑100 (MQ=255)
                                           gCTGTACTTCATTATCTTTCATTGGTACTTAGTCGCTTTAAAAAAGATTGAACTGCGTAAGTGCACTTATATAGATAACGAGGTTGGTGAACGAAttttt                                                     >  2:583808/1‑100 (MQ=255)
                                           gCTGAACTTTATTGTCTTTCATTGGTACTTACTAGAGTTAAAAAAGAGTGCACTGCGTAAGTGTCCTTATATTAATAACGAGGTTGGTCAACTAActtgt                                                     >  2:3405727/1‑98 (MQ=255)
                                            ccgatcTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAtttttt                                                    <  1:2928284/95‑1 (MQ=255)
                                             tGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCTCTGCGTAATTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTg                                                   <  2:3804853/100‑1 (MQ=255)
                                             tGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTg                                                   <  2:478636/100‑1 (MQ=255)
                                             tGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTg                                                   <  1:4087024/100‑1 (MQ=255)
                                              gTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAACAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGa                                                  <  2:3863705/100‑1 (MQ=255)
                                              gTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGa                                                  >  2:3517756/1‑100 (MQ=255)
                                              gTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGa                                                  <  2:850070/100‑1 (MQ=255)
                                              gTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGa                                                  >  1:1089490/1‑100 (MQ=255)
                                               tACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAc                                                 <  2:3451096/100‑1 (MQ=255)
                                               tACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAc                                                 <  2:3883904/100‑1 (MQ=255)
                                                aCTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACa                                                <  2:2757385/100‑1 (MQ=255)
                                                 cTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACAt                                               >  1:2615218/1‑100 (MQ=255)
                                                 cTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACAt                                               >  1:1029721/1‑100 (MQ=255)
                                                  tttATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATg                                              >  1:3493593/1‑100 (MQ=255)
                                                   ttATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGt                                             >  1:2180087/1‑100 (MQ=255)
                                                    tATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTa                                            <  2:2782595/100‑1 (MQ=255)
                                                     aTTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTACACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTGGACATGTAt                                           >  1:3558303/1‑100 (MQ=255)
                                                       tctcTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAAGAACGAGTTTGGTCAGCCAATTTTTTTTCATTTATca                                         >  2:3785745/1‑100 (MQ=255)
                                                       tctcTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGACCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATca                                         >  1:2538005/1‑100 (MQ=255)
                                                        ctctTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATcac                                        >  2:1359229/1‑100 (MQ=255)
                                                        ctctTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATcac                                        <  2:3163410/100‑1 (MQ=255)
                                                          ctTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACaa                                      >  1:1010797/1‑100 (MQ=255)
                                                          ctTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACaa                                      <  1:2809263/100‑1 (MQ=255)
                                                            ttCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAAt                                    >  2:3523740/1‑100 (MQ=255)
                                                             tCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAAtt                                   <  1:2396978/100‑1 (MQ=255)
                                                               aTTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTg                                 <  2:1187098/100‑1 (MQ=255)
                                                               aTTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTg                                 <  2:1117932/100‑1 (MQ=255)
                                                               aTTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTg                                 <  1:418009/100‑1 (MQ=255)
                                                                 tGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGaa                               <  2:2146567/100‑1 (MQ=255)
                                                                 tGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGaa                               <  1:1606430/100‑1 (MQ=255)
                                                                  ggTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAAt                              <  2:42188/100‑1 (MQ=255)
                                                                  ggTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAAACAATTTTTTGACATGTATAACAAATTTGAAt                              >  1:3980683/1‑100 (MQ=255)
                                                                   gTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATa                             <  2:3590897/100‑1 (MQ=255)
                                                                   gTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATa                             >  2:1582728/1‑100 (MQ=255)
                                                                   gTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATa                             <  1:3754995/100‑1 (MQ=255)
                                                                     aCTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCACTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGa                           <  2:989494/100‑1 (MQ=255)
                                                                      cTTCCTCGCTTTAAAAAAGAGTTCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGAt                          <  2:3453868/100‑1 (MQ=255)
                                                                      cTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGAt                          <  2:2052266/100‑1 (MQ=255)
                                                                      cTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGAt                          >  1:3129960/1‑100 (MQ=255)
                                                                         ccTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTTTCTTTTATCACAAATTTGAATAGATGTa                       >  2:2655842/1‑100 (MQ=255)
                                                                          cTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTAt                      <  1:1601494/100‑1 (MQ=255)
                                                                             gCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTAc                   >  1:612596/1‑100 (MQ=255)
                                                                                ttAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATc                <  1:774985/100‑1 (MQ=255)
                                                                                  aaaaaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCaa              >  1:740884/1‑100 (MQ=255)
                                                                                  aaaaaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCaa              <  1:358530/100‑1 (MQ=255)
                                                                                   aaaaaGAGTGCACTGCGTAAGTGCCCTTATGTAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAAc             <  1:1874212/100‑1 (MQ=255)
                                                                                   aaaaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAAc             <  1:1609790/100‑1 (MQ=255)
                                                                                   aaaaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAAc             >  2:1483925/1‑100 (MQ=255)
                                                                                    aaaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACt            <  1:3135846/100‑1 (MQ=255)
                                                                                     aaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTa           <  1:248509/100‑1 (MQ=255)
                                                                                          gTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCttt      <  1:3963308/100‑1 (MQ=255)
                                                                                          gTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCttt      <  1:371490/100‑1 (MQ=255)
                                                                                           tGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCtttt     >  2:2256038/1‑100 (MQ=255)
                                                                                            gCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTa    <  2:2481255/100‑1 (MQ=255)
                                                                                            gCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACGATCTTTTa    >  2:1471614/1‑100 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   >  1:3349686/1‑100 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   <  1:457549/100‑1 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   <  1:2395627/100‑1 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   >  1:2111681/1‑100 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   <  2:1537816/100‑1 (MQ=255)
                                                                                              aCTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAtt  <  1:3479119/100‑1 (MQ=255)
                                                                                              aCTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAtt  <  2:3721799/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATT  >  NC_000913/313178‑313371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: