BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,812,232	A→C	F110V (TTT→GTT)	yicR ←	UPF0758 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,812,232	0	A	C	98.2%	556.3 / ‑0.7	163	F110V (TTT→GTT)	yicR	UPF0758 family protein
Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (70/89); total (72/91)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 166 total aligned reads displayed.

AAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGC  >  NC_000913/3812136‑3812328

aaaaaaGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTc                                                                                               >  2:1160672/1‑100 (MQ=255)

 aaaaaGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTcc                                                                                              <  1:3055351/100‑1 (MQ=255)

 aaaaaGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTcc                                                                                              <  1:1643298/100‑1 (MQ=255)

  aaaaGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTccc                                                                                             <  1:1800463/100‑1 (MQ=255)

   aaaGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCt                                                                                            <  2:1416369/100‑1 (MQ=255)

     aGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCg                                                                                          <  1:1885872/100‑1 (MQ=255)

      gACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGt                                                                                         <  2:3466182/100‑1 (MQ=255)

        cGCCAATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTAACCCGTAAGCTGGCTTTGTAAAACTTCCCGCGTCa                                                                                       >  2:3375189/1‑100 (MQ=255)

           cTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCAtct                                                                                    >  1:3065306/1‑100 (MQ=255)

              tGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCg                                                                                 <  1:2630928/100‑1 (MQ=255)

              tGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCg                                                                                 <  1:375986/100‑1 (MQ=255)

               gCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCgg                                                                                >  1:1347596/1‑100 (MQ=255)

                cGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCggg                                                                               <  2:1551820/100‑1 (MQ=255)

                cGTTATAACCCGGTGTTCGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAACCTTCCCCCGTCATCTCCggg                                                                               <  2:1304529/100‑1 (MQ=255)

                 gTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGc                                                                              <  1:2077477/100‑1 (MQ=255)

                 gTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGc                                                                              <  2:509537/100‑1 (MQ=255)

                   tataACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTg                                                                            <  2:3241486/100‑1 (MQ=255)

                      aaCCCGGTGTTGGGAGTCGAGAAAGATCACCATAACGATCTCCCGCTCCTCACACGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGt                                                                         >  2:1811078/1‑100 (MQ=255)

                      aaCCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGt                                                                         >  2:1247301/1‑100 (MQ=255)

                      aaCCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGt                                                                         <  1:535612/100‑1 (MQ=255)

                       aCCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTa                                                                        >  2:3888901/1‑100 (MQ=255)

                        cccGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTaa                                                                       >  1:1330000/1‑100 (MQ=255)

                        cccGGTGTTGGGAGACGAGAAAGATCACCATAAAGATCTCCCGCTCCGCACCCGTGAGCTTGCTTTGTAAAACTTCCCTCGTCATCTCCTGTCTGCGTaa                                                                       >  2:1872036/1‑100 (MQ=255)

                         ccGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTaaa                                                                      >  1:1625279/1‑100 (MQ=255)

                           ggTGTTGGGAGTCGCGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCCTCTCCGGGCTGAGTAAAgg                                                                    <  2:3661292/100‑1 (MQ=255)

                           ggTGTTGGGAGTCGAGAAAGATCACCATAAAGGTCGCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAgg                                                                    <  1:2466909/100‑1 (MQ=255)

                           ggTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAgg                                                                    <  2:2046978/100‑1 (MQ=255)

                           ggTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAgg                                                                    <  1:2290466/100‑1 (MQ=255)

                           ggTGTTGGGAGTCGAGAAAGATATCCCTAAAGTTAGCCCGCTCCTCTCCCGTGAGATGGCTTTGTAATGTTTCCCTTGTCCTCTCCGGGCTGAGTaaacg                                                                    <  2:187020/100‑3 (MQ=255)

                             tgtTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGc                                                                  <  1:295316/100‑1 (MQ=255)

                              gtTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCt                                                                 >  2:352175/1‑100 (MQ=255)

                                tGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCttt                                                               >  1:3775098/1‑100 (MQ=255)

                                 gggAGTCGAGAGAGATCACCATGAAGATCTCGCGGGCCTGACCCGTGAGCTGGCTTTGGAAAACTTCCCTCGTTATGTCCGGGCTGCGTCAAGGGCTttc                                                              <  2:727413/100‑1 (MQ=255)

                                 gggAGTCGAGAAAGATCACCATAAAGTCCTCCCTCTCCTCCCCCGTGTGCTGGCTTTGTAAAACTTCCCTCGTCATCTACGGGCTGAGTAAAGGGCGttc                                                              <  2:2966054/100‑1 (MQ=255)

                                 gggAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttc                                                              >  1:1894216/1‑100 (MQ=255)

                                  ggAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttct                                                             <  2:3033141/100‑1 (MQ=255)

                                  ggAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttct                                                             >  1:3011213/1‑100 (MQ=255)

                                    aGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCTCCCGTGAGCTGGCTTTGTAAAGCTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttcttc                                                           <  2:1599747/100‑1 (MQ=255)

                                    aGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttcttc                                                           <  2:2604830/100‑1 (MQ=255)

                                    aGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttcttc                                                           <  2:2344174/100‑1 (MQ=255)

                                    aGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTttcttc                                                           >  1:1124780/1‑100 (MQ=255)

                                     gTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCa                                                          <  1:1732416/100‑1 (MQ=255)

                                     gTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCa                                                          >  1:169371/1‑100 (MQ=255)

                                      tCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCAc                                                         <  2:1429265/100‑1 (MQ=255)

                                       cGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACg                                                        <  1:122650/100‑1 (MQ=255)

                                       cGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACg                                                        >  1:3989253/1‑100 (MQ=255)

                                        gagaAAGATCACCATAAAGATCTCCCGCTCCTCAGCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGc                                                       >  2:85597/1‑100 (MQ=255)

                                        gagaAAGATCACCATAAAGAGCTCCCGCTCATCACCCGTGAGCTGGCGTTGTAAAACTGCCCTCGACATCGCCGGGCTCAGCAAAGGGCTTTCTTCAAGc                                                       >  2:3665293/1‑100 (MQ=255)

                                         agaAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCa                                                      >  2:2675990/1‑100 (MQ=255)

                                          gaAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCAt                                                     >  1:497515/1‑100 (MQ=255)

                                           aaaGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATc                                                    <  1:2529305/100‑1 (MQ=255)

                                             aGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCg                                                  <  1:2717284/100‑1 (MQ=255)

                                               aTCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCa                                                >  2:2562815/1‑100 (MQ=255)

                                                acgccATCAAGGTCACCCGCTCCTCCCCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGTCTGAGTAAAGGGCGTTCTTCACGCATCCGCAc                                               <  1:3264745/97‑1 (MQ=255)

                                                 cACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACg                                              <  1:1469360/100‑1 (MQ=255)

                                                  aCCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGt                                             >  2:1238932/1‑100 (MQ=255)

                                                   ccATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGtt                                            >  1:1851359/1‑100 (MQ=255)

                                                    cATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTg                                           <  1:894554/100‑1 (MQ=255)

                                                      tAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgta                                         >  1:1049900/1‑100 (MQ=255)

                                                      tAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgta                                         >  1:2986819/1‑100 (MQ=255)

                                                       aaaGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgtag                                        <  1:2288797/100‑1 (MQ=255)

                                                       aaaGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgtag                                        <  1:2172592/100‑1 (MQ=255)

                                                        aaGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgtagt                                       >  2:2211571/1‑100 (MQ=255)

                                                         aGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAGGGGCTTTCTTCACGCATCCGCACGTTgtagta                                      >  1:3740041/1‑100 (MQ=255)

                                                           aTCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAc                                    <  1:4057329/100‑1 (MQ=255)

                                                           aTCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAc                                    <  2:2942268/100‑1 (MQ=255)

                                                           aTCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAc                                    >  2:2869163/1‑100 (MQ=255)

                                                             ctcCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAAcgc                                  <  1:1016053/100‑1 (MQ=255)

                                                                    tcctcACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCa                           <  1:2820354/100‑1 (MQ=255)

                                                                     cctcACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAg                          >  1:2337887/1‑100 (MQ=255)

                                                                      ctcACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGTGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGt                         >  2:2406487/1‑100 (MQ=255)

                                                                      ctcACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGt                         >  1:2372577/1‑100 (MQ=255)

                                                                        cACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTc                       >  2:2399850/1‑100 (MQ=255)

                                                                        cACCCGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTc                       >  2:3044231/1‑100 (MQ=255)

                                                                          cccGTGCGCTGGCTTTGTAAAACTTCCCTCTGCATCTCCGTGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGAAACGCCGCGCCGGTTCAg                     <  1:3403894/100‑1 (MQ=255)

                                                                          cccGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAg                     <  2:1192723/100‑1 (MQ=255)

                                                                          cccGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCCCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAg                     >  2:2119698/1‑100 (MQ=255)

                                                                           ccGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGc                    <  1:1072952/100‑1 (MQ=255)

                                                                           ccGTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGc                    <  1:868226/100‑1 (MQ=255)

                                                                           ccGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGc                    <  2:487923/100‑1 (MQ=255)

                                                                             gTGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCaa                  <  1:2320339/100‑1 (MQ=255)

                                                                              tGAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAAt                 >  1:386764/1‑100 (MQ=255)

                                                                               gAGCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATc                >  2:1507705/1‑100 (MQ=255)

                                                                                 gCTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATccc              >  2:3494310/1‑100 (MQ=255)

                                                                                  cTGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATcccc             <  2:783495/100‑1 (MQ=255)

                                                                                   tGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCt            <  2:116834/100‑1 (MQ=255)

                                                                                   tGGCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCt            <  2:116937/100‑1 (MQ=255)

                                                                                    ggCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCtt           >  2:3957263/1‑100 (MQ=255)

                                                                                    ggCTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCtt           <  1:3165687/100‑1 (MQ=255)

                                                                                    ggCTTTATAAAACTTCCCTCCTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCtt           >  2:896842/1‑100 (MQ=255)

                                                                                      cTTTGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTa         <  2:817851/100‑1 (MQ=255)

                                                                                        ttGTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAAc       >  1:3950437/1‑100 (MQ=255)

                                                                                         tGTAAAACTTCCCTCGTCATCTCAGGGATGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGACGCGCCAGTTCAGCAATCCGCTTTaaat      >  2:288595/1‑98 (MQ=255)

                                                                                          gTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTg     >  1:2664515/1‑100 (MQ=255)

                                                                                          gTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTg     <  1:1728369/100‑1 (MQ=255)

                                                                                          gTAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTg     <  2:1919439/100‑1 (MQ=255)

                                                                                           tAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTgg    <  1:2400225/100‑1 (MQ=255)

                                                                                           tAAAACTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTgg    <  2:3237881/100‑1 (MQ=255)

                                                                                            aaaacTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTggg   >  1:2985161/1‑100 (MQ=255)

                                                                                             aaacTTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGc  <  2:615418/100‑1 (MQ=255)

AAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTTCAGCAATCCCCTTTAACTGGGC  >  NC_000913/3812136‑3812328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`