Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 14,011 T→G S617A (TCT→GCT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,0110TG94.7% 362.1 / 3.4 114S617A (TCT→GCT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (52/56);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 121 total aligned reads displayed.

TCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTG  >  NC_000913/13913‑14106
                                                                                                  |                                                                                               
tCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTc                                                                                                <  1:1118232/100‑1 (MQ=255)
  gAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGctgctg                                                                                              >  1:2835508/1‑100 (MQ=255)
  gAAGCGAAAATGCAGGAACTGGCACAGGTTTCACAGAAACTGATGGAAATCGCCCAGAAGCAACATGCACAGCAGCAGACTGCCGGTGCTGATGctgctg                                                                                              >  2:1705609/1‑100 (MQ=255)
    aGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCa                                                                                            <  1:66324/100‑1 (MQ=255)
    aGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCa                                                                                            <  1:1304363/100‑1 (MQ=255)
       gAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAaac                                                                                         <  1:2675985/100‑1 (MQ=255)
       gAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAaac                                                                                         <  1:1949429/100‑1 (MQ=255)
        aaaaTGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAaaca                                                                                        <  2:3267427/100‑1 (MQ=255)
         aaaTGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAaacaa                                                                                       <  2:2244957/100‑1 (MQ=255)
          aaTGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAaacaac                                                                                      <  2:2038859/100‑1 (MQ=255)
          aaTGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATCACCAGCAGCAGACTGCCGGTGCTGACGCTGCTGCAaacaac                                                                                      >  2:3814522/1‑100 (MQ=255)
           aTGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAAcg                                                                                     <  2:3444030/100‑1 (MQ=255)
              cAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGa                                                                                  >  1:3715550/1‑100 (MQ=255)
              cAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGa                                                                                  >  2:708826/1‑100 (MQ=255)
              cAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGa                                                                                  <  1:2918741/100‑1 (MQ=255)
              cAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGa                                                                                  >  2:1490518/1‑100 (MQ=255)
                ggAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGTCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGaaa                                                                                >  2:3463862/1‑100 (MQ=255)
                ggAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGaaa                                                                                <  1:2705662/100‑1 (MQ=255)
                ggAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGaaa                                                                                >  1:2926039/1‑100 (MQ=255)
                     tGGCACAGGTTGCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGa                                                                           <  2:3777966/100‑1 (MQ=255)
                      ggCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGAc                                                                          >  1:1784462/1‑100 (MQ=255)
                       gCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCCGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACg                                                                         <  1:191171/100‑1 (MQ=255)
                       gCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACg                                                                         <  1:3087306/100‑1 (MQ=255)
                       gCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACg                                                                         <  2:1076255/100‑1 (MQ=255)
                           aGGTTCCCCAGAAACTGATGGAAATCGCGCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgt                                                                     <  2:3438188/100‑1 (MQ=255)
                            ggTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGCtgtt                                                                    >  1:3336228/1‑100 (MQ=255)
                            ggTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgtt                                                                    >  1:1111766/1‑100 (MQ=255)
                            ggTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgtt                                                                    >  2:3530823/1‑100 (MQ=255)
                            ggTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgtt                                                                    >  1:1546587/1‑100 (MQ=255)
                            ggTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgtt                                                                    >  2:3704832/1‑100 (MQ=255)
                             gTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgttg                                                                   >  2:2633607/1‑100 (MQ=255)
                              tttGCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGAtgttgt                                                                  >  2:1746952/1‑100 (MQ=255)
                                 cccAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGa                                                               >  1:1864496/1‑100 (MQ=255)
                                  ccAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGAc                                                              <  2:3900674/100‑1 (MQ=255)
                                  ccAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGAc                                                              <  2:1666049/100‑1 (MQ=255)
                                  ccAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGAc                                                              <  1:2329660/100‑1 (MQ=255)
                                  ccAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGAc                                                              >  1:1925371/1‑100 (MQ=255)
                                   cAGAAATTGATGGAAATGGCCCAGCAGCAACATGCACAGCAGCAGACTGCGGGTGCTGATGCTGCTGCAAACAAGGCGAAGGATGACGATGTTGTCGACg                                                             >  2:2325345/1‑100 (MQ=255)
                                   cAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACg                                                             >  2:2324873/1‑100 (MQ=255)
                                    aGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGc                                                            >  1:818999/1‑100 (MQ=255)
                                     gAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCt                                                           >  2:692023/1‑100 (MQ=255)
                                         cTGAGGGAAATCGCCCAGCAGAAACATGCCAAGCAGCAGACTGCCGGAGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAt                                                       <  2:2108119/100‑1 (MQ=255)
                                         cTGACGGAAATCGCCCAGCAGCAACATGCCCAGCAGCATACTTCCGGTGCTGATGCTGCTGCAAACATCGCGAAAGATGACGATGTTGTCGACGCTGAAt                                                       <  2:2834840/100‑1 (MQ=255)
                                          tGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAtt                                                      <  1:3301355/100‑1 (MQ=255)
                                          tGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAtt                                                      <  2:2128079/100‑1 (MQ=255)
                                            aTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGGTGACGATGGTGGTTACTCTGAAttgg                                                    >  1:187877/1‑98 (MQ=255)
                                            aTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTg                                                    >  2:908088/1‑100 (MQ=255)
                                            aTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTg                                                    >  1:1286052/1‑100 (MQ=255)
                                              ggAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGAAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaa                                                  <  2:1261014/100‑1 (MQ=255)
                                               taaaGCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaag                                                 <  1:3287349/99‑1 (MQ=255)
                                               gAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaag                                                 <  1:2099269/100‑1 (MQ=255)
                                                aaaTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaaga                                                >  1:2570404/1‑100 (MQ=255)
                                                 aaTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaagaa                                               >  1:2819228/1‑100 (MQ=255)
                                                  aTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGaagaag                                              <  2:3195894/100‑1 (MQ=255)
                                                  aTCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGaagaag                                              <  2:2757246/100‑1 (MQ=255)
                                                   tCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGt                                             <  1:724062/100‑1 (MQ=255)
                                                   tCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGt                                             <  2:2010089/100‑1 (MQ=255)
                                                     gCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCa                                           <  1:1579161/100‑1 (MQ=255)
                                                      cccAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAAGGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCaa                                          >  1:3742169/1‑100 (MQ=255)
                                                      cccAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCaa                                          >  2:431042/1‑100 (MQ=255)
                                                       ccAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCaaa                                         >  2:214120/1‑100 (MQ=255)
                                                        cagcagCAACATGCCCAGCAGCAGACTGCCGGTTCTGAGGCTGCGGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAg                                        <  2:3200390/100‑1 (MQ=255)
                                                         agcagcAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGa                                       >  1:3821845/1‑100 (MQ=255)
                                                         agcagcAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGa                                       >  1:585285/1‑100 (MQ=255)
                                                          gcagcaACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGAc                                      <  2:1381944/100‑1 (MQ=255)
                                                                aCATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACaaaaaa                                <  1:2064524/100‑1 (MQ=255)
                                                                 cATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAaat                               <  1:2946607/100‑1 (MQ=255)
                                                                   tGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAaataa                             <  2:1896931/100‑1 (MQ=255)
                                                                   tGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAaataa                             >  1:3799977/1‑100 (MQ=255)
                                                                     cccAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATc                           >  1:240150/1‑100 (MQ=255)
                                                                     cccAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATc                           <  1:41056/100‑1 (MQ=255)
                                                                     cccAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATc                           >  1:806560/1‑100 (MQ=255)
                                                                      ccAGCAGCAGACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCg                          <  1:3608441/100‑1 (MQ=255)
                                                                         gcagcagACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGccc                       <  1:79567/100‑1 (MQ=255)
                                                                         gcagcagACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGccc                       >  2:2331916/1‑100 (MQ=255)
                                                                            gcagACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCtat                    >  1:2143730/1‑100 (MQ=255)
                                                                              agACTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATaa                  >  2:516025/1‑100 (MQ=255)
                                                                                aCTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAAc                <  2:1046989/100‑1 (MQ=255)
                                                                                 cTGCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACg               <  1:50485/100‑1 (MQ=255)
                                                                                   gCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCCGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACggg             <  2:3010477/100‑1 (MQ=255)
                                                                                   gCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACggg             <  1:2055111/100‑1 (MQ=255)
                                                                                   gCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACggg             >  2:697613/1‑100 (MQ=255)
                                                                                   gCCGGTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACggg             >  1:1676208/1‑100 (MQ=255)
                                                                                       gTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAAt         <  1:2656463/100‑1 (MQ=255)
                                                                                       gTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAAt         <  1:465071/100‑1 (MQ=255)
                                                                                       gTGCTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAAt         <  1:2634230/100‑1 (MQ=255)
                                                                                        tGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGTTAAtt        >  2:2704603/1‑100 (MQ=255)
                                                                                          cTGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAAAAAAAAAAAAAAAATAATCGCCCTATAAACGGGTAATtat      <  2:3052160/100‑1 (MQ=255)
                                                                                           tGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATtata     >  1:2524116/1‑100 (MQ=255)
                                                                                           tGATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATtata     >  2:317949/1‑100 (MQ=255)
                                                                                            gATGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTAGACGCTGAATTTGAAGAAGTCCAAGACAAAAAATAATCGCCCTATAAACGGGAACTTATAc    >  1:1010182/1‑100 (MQ=255)
                                                                                             aTGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATAc  <  2:3603446/100‑2 (MQ=255)
                                                                                             aTGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACt   >  2:3675016/1‑100 (MQ=255)
                                                                                             aTGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACt   <  1:2695334/100‑1 (MQ=255)
                                                                                             aTGCTGCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACt   <  2:55323/100‑1 (MQ=255)
                                                                                              tgctgctgcAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg  >  1:154044/1‑100 (MQ=255)
                                                                                              tgctgctgcAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg  <  1:735815/100‑1 (MQ=255)
                                                                                              tgctgctgcAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg  <  2:529507/100‑1 (MQ=255)
                                                                                              tgctgctgcAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg  <  1:1366272/100‑1 (MQ=255)
                                                                                              tGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTg  >  2:54848/1‑100 (MQ=255)
                                                                                                  |                                                                                               
TCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTG  >  NC_000913/13913‑14106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: