BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,808	T→G	L444R (CTG→CGG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,808	0	T	G	94.9%	575.3 / 11.2	177	L444R (CTG→CGG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base T (4/5); new base G (87/81); total (91/86)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCAA  >  NC_000913/4216713‑4216904

aCGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATg                                                                                              >  2:135186/1‑100 (MQ=255)

 cGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATgg                                                                                             >  1:3478239/1‑100 (MQ=255)

   cACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGaa                                                                                           <  2:2220251/100‑1 (MQ=255)

   cACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGaa                                                                                           >  1:1489286/1‑100 (MQ=255)

   cACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGaa                                                                                           >  1:1900857/1‑100 (MQ=255)

     ncGAAGAAGGTATGCGAGCCAACATTCGCGTGGCCGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCGATGTGCCGATTTATCACCGGAGGGAAGa                                                                                         >  2:2957944/2‑100 (MQ=255)

       gaagaaGGTATGCGCGCGAACAGTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATg                                                                                       <  2:571519/100‑1 (MQ=255)

       gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATg                                                                                       >  2:619085/1‑100 (MQ=255)

       gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATg                                                                                       >  2:1732635/1‑100 (MQ=255)

        aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATgc                                                                                      >  1:940624/1‑100 (MQ=255)

         agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATgcg                                                                                     >  2:2496640/1‑100 (MQ=255)

           aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATgcggc                                                                                   <  2:247868/100‑1 (MQ=255)

           aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATgcggc                                                                                   <  2:2816274/100‑1 (MQ=255)

            agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATgcggcg                                                                                  <  2:1725885/100‑1 (MQ=255)

             ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGa                                                                                 >  1:320170/1‑100 (MQ=255)

             ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGa                                                                                 >  1:2825485/1‑100 (MQ=255)

             ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATCTATGGCCGGATGGACGATGCGGCGa                                                                                 >  2:1167472/1‑100 (MQ=255)

              gTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGAc                                                                                <  1:1263998/100‑1 (MQ=255)

                 tGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGc                                                                             <  1:1023918/100‑1 (MQ=255)

                 tGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGc                                                                             <  1:3913080/100‑1 (MQ=255)

                 tGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGc                                                                             >  1:3434771/1‑100 (MQ=255)

                  gcgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCt                                                                            >  1:1352314/1‑100 (MQ=255)

                   cgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTg                                                                           >  2:1850157/1‑100 (MQ=255)

                         aaCATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAttt                                                                     <  2:3714045/100‑1 (MQ=255)

                          aCATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTc                                                                    <  1:330487/100‑1 (MQ=255)

                          aCATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACCGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTc                                                                    <  1:1405775/100‑1 (MQ=255)

                             ttCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCg                                                                 <  1:471983/100‑1 (MQ=255)

                               cgcgTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTa                                                               <  1:632209/100‑1 (MQ=255)

                                 cgTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc                                                             >  2:246130/1‑100 (MQ=255)

                                 cgTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc                                                             >  2:1217775/1‑100 (MQ=255)

                                 cgTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc                                                             <  1:1534647/100‑1 (MQ=255)

                                   tGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc                                                           >  2:3241841/1‑100 (MQ=255)

                                    ggCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCg                                                          <  2:1939232/100‑1 (MQ=255)

                                     gCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATAGCCGGATGGAAGATGCGGAGACGGGTGAAATTTCCCGTACCTCGa                                                         >  1:3822563/1‑100 (MQ=255)

                                       tgtgCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc                                                       >  2:3205299/1‑100 (MQ=255)

                                          gCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTgg                                                    >  1:1644245/1‑100 (MQ=255)

                                          gCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTgg                                                    >  1:3528426/1‑100 (MQ=255)

                                           cAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc                                                   <  2:281226/100‑1 (MQ=255)

                                            aGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCa                                                  >  1:3954209/1‑100 (MQ=255)

                                            aGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCa                                                  >  2:3230539/1‑100 (MQ=255)

                                            aGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATCTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCa                                                  >  2:239181/1‑100 (MQ=255)

                                             gTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGTAATTTCCCGTCCCTCGATCTGGCAg                                                 >  2:2154250/1‑100 (MQ=255)

                                             gTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAg                                                 <  1:1568372/100‑1 (MQ=255)

                                             gTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAg                                                 >  1:3110989/1‑100 (MQ=255)

                                             gTACATCGAAGCGTGGATCTCTGGCAACGGCTGCGTGCCGATTTATGGCCGGCTGGAAGATGCGGCGACGGGTGAAATTTCCCGTACCTCGATCTTGCAg                                                 >  2:3312696/1‑100 (MQ=255)

                                             gTACATCGAAGCGTGGATCTCTGGCAACAGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAg                                                 <  1:3707794/100‑1 (MQ=255)

                                               aCATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAGTTTCCCGTACCTCGATCTGGCAGTg                                               <  1:2228265/100‑1 (MQ=255)

                                               aCATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTg                                               <  1:3868058/100‑1 (MQ=255)

                                               aCATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTg                                               <  1:926271/100‑1 (MQ=255)

                                                cATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTgg                                              >  1:1761044/1‑100 (MQ=255)

                                                cATCGAAGCGAGGATCTCTGGCCACGGCTGTGTGCTGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTCCGCGTACCTCGATCTGGCAGTgg                                              <  2:2220978/100‑1 (MQ=255)

                                                 aTCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGa                                             >  1:1136875/1‑100 (MQ=255)

                                                   cGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATc                                           <  2:1870476/100‑1 (MQ=255)

                                                    gAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATcc                                          <  1:2133185/100‑1 (MQ=255)

                                                       gCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCcatc                                       <  1:2988890/100‑1 (MQ=255)

                                                       gCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCcatc                                       <  2:1492517/100‑1 (MQ=255)

                                                        cGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCcatca                                      >  1:512076/1‑100 (MQ=255)

                                                          tGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCcatcatn                                    <  1:1844580/100‑2 (MQ=255)

                                                           tccgatctGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCAtcatca                                   <  2:2080993/95‑1 (MQ=255)

                                                           ggATCTCTCGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCAtcatca                                   >  2:3832151/1‑100 (MQ=255)

                                                            gATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCaa                                  >  1:2777399/1‑100 (MQ=255)

                                                            gATCTCTGGCAACGGCTGTGTGCCGATGTATGGCCGGATGGAAGATGCGGCGACCGCTGTAATTTCCCGAACCTCGATCTGGCATTGGATCCATCATCaa                                  <  1:3544812/100‑1 (MQ=255)

                                                             atgtctGGCAACGGCTGTGTGCCGATGTATGTCCGCATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCTTCAGCaaa                                 <  2:837376/97‑1 (MQ=255)

                                                              tctcTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCaaaa                                >  1:1542253/1‑100 (MQ=255)

                                                              tctcTGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCaaaa                                >  1:2754926/1‑100 (MQ=255)

                                                                tctGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCaaaaaa                              <  1:613451/100‑1 (MQ=255)

                                                                 ctGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAAc                             >  2:3752160/1‑100 (MQ=255)

                                                                  tGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACg                            <  1:1280223/100‑1 (MQ=255)

                                                                  tGGCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACg                            <  2:1146158/100‑1 (MQ=255)

                                                                  tGGCAACGGCGGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACg                            <  1:3970107/100‑1 (MQ=255)

                                                                   ggCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGt                           <  2:1093505/100‑1 (MQ=255)

                                                                    gCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGtt                          <  2:2713147/100‑1 (MQ=255)

                                                                    gCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGtt                          >  2:2200117/1‑100 (MQ=255)

                                                                    gCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGtt                          <  2:1132063/100‑1 (MQ=255)

                                                                    gCAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGtn                          <  1:3246900/100‑2 (MQ=255)

                                                                     cAACGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTg                         <  1:3351554/100‑1 (MQ=255)

                                                                      aaCGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGa                        <  2:431766/100‑1 (MQ=255)

                                                                       aCGGCTGTGTGGCGATTTATGGCCGGATGGAATATGGGGCGACGGGTGAAATTTCCCGTACCTCGATCTGGCAGGGGATCCATCATCAAAAAACGTTGAg                       <  2:3183832/100‑1 (MQ=255)

                                                                       aCGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGan                       <  1:3590402/100‑2 (MQ=255)

                                                                       aCGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTAAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAg                       >  1:3648163/1‑100 (MQ=255)

                                                                        cGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGc                      <  1:3837496/100‑1 (MQ=255)

                                                                        cGGCTGTGTGCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGc                      <  2:2593351/100‑1 (MQ=255)

                                                                           cTGTTTGCCTATTTATGGCCGGATGGAAGATGCGGCGACGGCGGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAAt                   <  1:2031492/100‑1 (MQ=255)

                                                                               gtgCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCa               >  2:2052408/1‑100 (MQ=255)

                                                                               gtgCCGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCa               <  1:2665217/100‑1 (MQ=255)

                                                                                  ccGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAc            >  1:1308151/1‑100 (MQ=255)

                                                                                  ccGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAc            <  2:671039/100‑1 (MQ=255)

                                                                                  ccGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAc            >  2:3553741/1‑100 (MQ=255)

                                                                                   cGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAcc           >  2:203894/1‑100 (MQ=255)

                                                                                   cGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAcc           >  1:2607297/1‑100 (MQ=255)

                                                                                   cGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAcc           >  1:2683847/1‑100 (MQ=255)

                                                                                   cGATTTATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAAcc           <  1:1984542/100‑1 (MQ=255)

                                                                                       ttATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTg       <  2:180498/100‑1 (MQ=255)

                                                                                       ttATGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTg       <  1:68115/100‑1 (MQ=255)

                                                                                          tGGCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGAcc    <  2:1270911/100‑1 (MQ=255)

                                                                                           ggCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCa   >  2:703765/1‑100 (MQ=255)

                                                                                            gCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCaa  >  1:707989/1‑100 (MQ=255)

                                                                                            gCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCaa  >  1:3206539/1‑100 (MQ=255)

                                                                                            gCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCaa  >  1:734334/1‑100 (MQ=255)

                                                                                            gCCGGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCaa  >  2:2293339/1‑100 (MQ=255)

ACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCAA  >  NC_000913/4216713‑4216904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`