Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,285,713 A→C N553K (AAT→AAG acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,285,7130AC98.3% 610.7 / ‑0.4 176N553K (AAT→AAGacsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (88/85);  total (90/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 176 total aligned reads displayed.

TTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACG  >  NC_000913/4285618‑4285809
                                                                                               |                                                                                                
ttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                              >  2:2356468/1‑100 (MQ=255)
ttACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgt                                                                                              <  2:2674049/100‑1 (MQ=255)
 tACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTtgtg                                                                                             >  2:3563945/1‑100 (MQ=255)
   cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCg                                                                                           >  1:3788760/1‑100 (MQ=255)
   cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCg                                                                                           >  1:3804224/1‑100 (MQ=255)
   cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCg                                                                                           >  1:1985147/1‑100 (MQ=255)
   cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCg                                                                                           <  2:3706138/100‑1 (MQ=255)
    gCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCgg                                                                                          <  1:1030286/100‑1 (MQ=255)
    gCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCgg                                                                                          <  1:4038237/100‑1 (MQ=255)
     cACCGAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                         <  1:3902162/100‑1 (MQ=255)
     cACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGa                                                                                         >  1:1249520/1‑100 (MQ=255)
       cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAAt                                                                                       <  2:3031601/100‑1 (MQ=255)
        ccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATa                                                                                      >  2:2222874/1‑100 (MQ=255)
        ccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATa                                                                                      <  1:3607478/100‑1 (MQ=255)
          aGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATAcc                                                                                    <  2:3902922/100‑1 (MQ=255)
           gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACct                                                                                   <  1:2574777/100‑1 (MQ=255)
             tGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctac                                                                                 >  1:334792/1‑100 (MQ=255)
             tGCGGACTTCTGAGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctac                                                                                 >  1:3516541/1‑100 (MQ=255)
              gCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctact                                                                                <  2:306703/100‑1 (MQ=255)
               cGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACctacta                                                                               >  1:1359632/1‑100 (MQ=255)
                   cTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggc                                                                           >  1:1391169/1‑100 (MQ=255)
                    ttCTGCGTACCGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcg                                                                          <  2:2770040/100‑1 (MQ=255)
                     tCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcgg                                                                         >  2:1878520/1‑100 (MQ=255)
                     tCTGCGAACAGTTCTGGTAACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTAcggcgg                                                                         >  1:3406731/1‑100 (MQ=255)
                      cTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACggcggc                                                                        <  1:461737/100‑1 (MQ=255)
                       tGCGTACAGTTCTGGTGGCGGTGCCTCCCCTTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCt                                                                       <  2:1169064/100‑1 (MQ=255)
                         cGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTc                                                                     >  2:2524003/1‑100 (MQ=255)
                           tACGGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                   <  1:2428257/100‑1 (MQ=255)
                           tACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                   <  1:3159050/100‑1 (MQ=255)
                            aCAGTTGTTGGGACGGTTCCTCCCCGGGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGTAATACCTACTACGGCGGCTTCGGc                                                                  <  2:1812038/100‑1 (MQ=255)
                            aCAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGc                                                                  <  2:553910/100‑1 (MQ=255)
                             cAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCa                                                                 <  1:4064693/100‑1 (MQ=255)
                             cAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCa                                                                 >  2:169718/1‑100 (MQ=255)
                                 gcTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCt                                                             <  2:2441116/99‑1 (MQ=255)
                                   tGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTc                                                           >  2:1680064/1‑100 (MQ=255)
                                    ggTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                          <  1:961143/100‑1 (MQ=255)
                                    ggTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCg                                                          >  1:827123/1‑100 (MQ=255)
                                      tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                        <  1:3408641/100‑1 (MQ=255)
                                      tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGa                                                        <  1:55014/100‑1 (MQ=255)
                                       gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                       <  2:80412/100‑1 (MQ=255)
                                       gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                       <  1:4008536/100‑1 (MQ=255)
                                       gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                       >  2:917529/1‑100 (MQ=255)
                                       gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                       >  1:1054570/1‑100 (MQ=255)
                                       gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                       <  1:2941012/100‑1 (MQ=255)
                                          ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcg                                                    >  1:1073821/1‑100 (MQ=255)
                                          ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcg                                                    <  2:809728/100‑1 (MQ=255)
                                          ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcg                                                    <  1:357911/100‑1 (MQ=255)
                                           gTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcgc                                                   <  1:2800126/100‑1 (MQ=255)
                                              cctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccac                                                >  2:2107726/1‑100 (MQ=255)
                                                  cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgc                                            >  1:1844613/1‑100 (MQ=255)
                                                   ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcg                                           >  2:1970491/1‑100 (MQ=255)
                                                   ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcg                                           >  2:689867/1‑100 (MQ=255)
                                                     gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                         <  1:2001904/100‑1 (MQ=255)
                                                     gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGcc                                         >  1:2611570/1‑100 (MQ=255)
                                                      tGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCCATCCTCGGATGCGCCACCAGCGCCg                                        >  1:2856262/1‑100 (MQ=255)
                                                        gttAAGCGTGGCGTTGGCATAGATCGTCTGCCCTTTACGCTTGGGCGTAATGCCCACAACCACGCCTCCGGGCATCCTCGGATGCGCCACCAGCGCTGag                                      >  2:616255/2‑99 (MQ=255)
                                                        aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGAc                                      <  1:2345140/100‑1 (MQ=255)
                                                        aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGAc                                      >  2:2155305/1‑100 (MQ=255)
                                                        aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGAc                                      <  1:1515086/100‑1 (MQ=255)
                                                         ttAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACt                                     <  2:175472/100‑1 (MQ=255)
                                                           aaGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCa                                   >  2:1111577/1‑100 (MQ=255)
                                                            aGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCaa                                  >  1:722914/1‑100 (MQ=255)
                                                            aGCGGGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCaa                                  <  1:119753/100‑1 (MQ=255)
                                                             gCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAt                                 <  1:1249806/100‑1 (MQ=255)
                                                             gCGTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAt                                 >  1:2721460/1‑100 (MQ=255)
                                                               gTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtct                               <  2:2789025/100‑1 (MQ=255)
                                                               gTGACGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAtct                               <  2:830270/100‑1 (MQ=255)
                                                                  aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                            <  2:3161460/100‑1 (MQ=255)
                                                                  aCGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTg                            <  1:632720/100‑1 (MQ=255)
                                                                   cGTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGc                           <  2:2926291/100‑1 (MQ=255)
                                                                    gTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                          <  1:1788249/100‑1 (MQ=255)
                                                                    gTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                          <  2:534680/100‑1 (MQ=255)
                                                                    gTAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                          <  1:2092678/100‑1 (MQ=255)
                                                                     tAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCg                         <  2:1644541/100‑1 (MQ=255)
                                                                     tAGGCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCg                         >  1:592187/1‑100 (MQ=255)
                                                                       ggCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTc                       >  1:3975711/1‑100 (MQ=255)
                                                                        gCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcc                      >  2:3499266/1‑100 (MQ=255)
                                                                        gCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcc                      >  1:3759789/1‑100 (MQ=255)
                                                                        gCGTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcc                      >  1:2438306/1‑100 (MQ=255)
                                                                          gTAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcccc                    >  1:2819248/1‑100 (MQ=255)
                                                                          gTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTcccc                    <  1:172579/100‑1 (MQ=255)
                                                                           tAGATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCa                   >  1:3159943/1‑100 (MQ=255)
                                                                             gATCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGa                 >  2:1794686/1‑100 (MQ=255)
                                                                              aTCGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGAc                <  1:3777170/100‑1 (MQ=255)
                                                                                cGCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACgg              <  1:542903/100‑1 (MQ=255)
                                                                                 gCCTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGt             >  2:1464535/1‑100 (MQ=255)
                                                                                 gCCTGACCTTTAATCTTGTGCGGAATACCCACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGt             >  1:3399172/1‑100 (MQ=255)
                                                                                  ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTg            >  1:785190/1‑100 (MQ=255)
                                                                                  ccTGACCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTg            <  2:3656749/100‑1 (MQ=255)
                                                                                    tGACCTTTAATCTTGTGCGGAATACCTCCTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCCGCGCCGACTCACAGTCTGCCGTCCCCAGACGGTGAc          <  2:1545057/100‑1 (MQ=255)
                                                                                      aCCTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg        <  1:212984/100‑1 (MQ=255)
                                                                                       ccTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCgg       >  1:1606550/1‑100 (MQ=255)
                                                                                        ntttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga      >  1:3232799/2‑100 (MQ=255)
                                                                                        cgttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAACCTCTGCCGTCTCCAGACGGTAACCGga      >  2:160345/3‑100 (MQ=255)
                                                                                        cTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga      >  1:3698619/1‑100 (MQ=255)
                                                                                        cTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga      >  2:1245807/1‑100 (MQ=255)
                                                                                        cTTTAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGga      <  1:2241913/100‑1 (MQ=255)
                                                                                          ttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGCCGGTGACCGgaga    >  2:3771364/1‑100 (MQ=255)
                                                                                          ttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga    <  2:864979/100‑1 (MQ=255)
                                                                                          ttAATCTTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga    >  1:2134132/1‑100 (MQ=255)
                                                                                               |                                                                                                
TTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACG  >  NC_000913/4285618‑4285809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: