Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,291,339 T→G I288M (ATT→ATG nrfD → formate‑dependent nitrite reductase, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,291,3390TG96.4% 551.9 / 2.0 165I288M (ATT→ATGnrfDformate‑dependent nitrite reductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (5/1);  new base G (88/71);  total (93/72)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

CGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCC  >  NC_000913/4291243‑4291435
                                                                                                |                                                                                                
cGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCt                                                                                               >  2:98336/1‑100 (MQ=255)
 ggTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTg                                                                                              >  2:3973142/1‑100 (MQ=255)
 ggTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTg                                                                                              <  1:1229116/100‑1 (MQ=255)
   tttCTGGACGTGGTGGCTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCAtgcctgcc                                                                                            <  2:36582/100‑1 (MQ=255)
     tCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGt                                                                                          <  1:1038241/100‑1 (MQ=255)
       tGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGGCAATCGCAGTTCCGGCATGCCTGCCGTGc                                                                                        >  1:497269/1‑100 (MQ=255)
        ggACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCt                                                                                       >  2:2480935/1‑100 (MQ=255)
        ggACGTGGTGGTTCTGGCTTGGTGTCGCCGGGATGGGGCTGATTGTACCAATGCTGCTCAAACCGTGGGTCAATCGCAGTACCGGCATTCCTGCCGTGCt                                                                                       >  1:3627486/1‑100 (MQ=255)
           cGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggc                                                                                    >  1:1582298/1‑100 (MQ=255)
            gtggtgGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcg                                                                                   <  1:841539/100‑1 (MQ=255)
            gtggtgGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcg                                                                                   >  2:2006530/1‑100 (MQ=255)
            gtggtgGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcg                                                                                   <  1:2162370/100‑1 (MQ=255)
             tggtggTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcgg                                                                                  >  1:3686237/1‑100 (MQ=255)
              ggtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCCTGCCTGCCGTGCTggcggc                                                                                 >  2:3574402/1‑100 (MQ=255)
              ggtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTggcgcc                                                                                 >  2:2299120/1‑98 (MQ=255)
               gtggtTCTGGCTTGGTGTGGCCGGGCTGGGGCTGATTGTGGCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGgcggcg                                                                                <  2:3475330/100‑1 (MQ=255)
               gtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCTCAGTTCCGGCATGCCTGCCGTGCTGgcggcg                                                                                >  2:1654891/1‑100 (MQ=255)
               gtggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGgcggcg                                                                                <  1:3531461/100‑1 (MQ=255)
                tggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgt                                                                               >  1:2779965/1‑100 (MQ=255)
                 ggtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtg                                                                              >  1:413796/1‑100 (MQ=255)
                  gtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtgt                                                                             >  1:1119616/1‑100 (MQ=255)
                  gtTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCgtgt                                                                             <  1:2084820/100‑1 (MQ=255)
                   ttCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGtgtg                                                                            >  2:2516446/1‑100 (MQ=255)
                   ttCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGtgtg                                                                            <  1:2519699/100‑1 (MQ=255)
                   ttCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGtgtg                                                                            <  2:1735440/100‑1 (MQ=255)
                   ttCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGtgtg                                                                            >  1:597545/1‑100 (MQ=255)
                    tCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAATCCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgg                                                                           >  2:3784857/1‑100 (MQ=255)
                    tCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgg                                                                           <  2:471842/100‑1 (MQ=255)
                    tCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgg                                                                           >  2:3169915/1‑100 (MQ=255)
                     cTGGCTTGGTGTCGCGGGGGTGGGGCTGATTGTGCCAATGATGCTCACACAGTGGGTCAATAGCAGTTCAGGCATGCCTGACGTGATGGCGGAGTGTggg                                                                          >  2:2830626/1‑100 (MQ=255)
                      tGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgggg                                                                         <  2:179798/100‑1 (MQ=255)
                      tGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGGCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTgggg                                                                         <  2:3814563/100‑1 (MQ=255)
                       ggCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGc                                                                        <  2:1981587/100‑1 (MQ=255)
                       ggCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGc                                                                        >  1:1310834/1‑100 (MQ=255)
                         cTTGGTGTCGCCGGGTTGGGGCTGATTGTGCCAATGCTGGTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGACGTGCTGGCGGCGCGTGGGGCCa                                                                      >  1:3555101/1‑100 (MQ=255)
                         cTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCa                                                                      >  2:1414063/1‑100 (MQ=255)
                         cTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCa                                                                      >  1:354544/1‑100 (MQ=255)
                             gtgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCt                                                                  >  1:1042573/1‑100 (MQ=255)
                             gtgtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCt                                                                  >  2:129039/1‑100 (MQ=255)
                               gtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTgg                                                                >  1:880499/1‑100 (MQ=255)
                               gtCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCGCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTgg                                                                <  2:3174659/100‑1 (MQ=255)
                                 cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                              >  1:3299205/1‑100 (MQ=255)
                                 cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                              >  1:1379867/1‑100 (MQ=255)
                                 cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                              <  2:2264077/100‑1 (MQ=255)
                                 cGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTc                                                              >  1:1296615/1‑100 (MQ=255)
                                  gCCGGGCTGGGGCTGATTGTGCGAATGCTGTTGAAACGGTGGGGCAAGCGCAGTACCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCg                                                             <  2:2925340/100‑1 (MQ=255)
                                   ccGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCgg                                                            >  2:1847369/1‑100 (MQ=255)
                                     gggctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCg                                                          <  2:3607701/100‑1 (MQ=255)
                                        ctggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCgtgn                                                       <  1:256120/100‑2 (MQ=255)
                                         tggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGtt                                                      <  2:335611/100‑1 (MQ=255)
                                         tggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGtt                                                      >  1:3892718/1‑100 (MQ=255)
                                          ggggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTg                                                     >  2:936554/1‑100 (MQ=255)
                                           gggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGa                                                    <  2:970586/100‑1 (MQ=255)
                                            ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAt                                                   >  1:2196506/1‑100 (MQ=255)
                                            ggctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAt                                                   <  2:687641/100‑1 (MQ=255)
                                              ctgATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgc                                                 >  2:2270661/1‑100 (MQ=255)
                                                gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctg                                               >  2:320493/1‑100 (MQ=255)
                                                gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctg                                               >  1:3505499/1‑100 (MQ=255)
                                                gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctg                                               >  2:439717/1‑100 (MQ=255)
                                                gATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctg                                               >  2:752081/1‑100 (MQ=255)
                                                 aTTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGAtgctgc                                              >  2:3699766/1‑100 (MQ=255)
                                                  ttGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTgcg                                             >  1:362076/1‑100 (MQ=255)
                                                      gCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCttt                                         >  1:1672887/1‑100 (MQ=255)
                                                      gCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGGGTTGATGCTGCGCttt                                         >  2:2723326/1‑100 (MQ=255)
                                                       ccAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCtttt                                        >  1:2620530/1‑100 (MQ=255)
                                                          aTGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTATGGTCGGCGTGTTGATGCTGCGCTTTTTCa                                     >  1:3811765/1‑100 (MQ=255)
                                                           tgctgcTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAt                                    >  1:1709405/1‑100 (MQ=255)
                                                           tgctgcTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAt                                    <  1:2978359/100‑1 (MQ=255)
                                                           tgctgcTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAt                                    <  2:604932/100‑1 (MQ=255)
                                                           tgctgcTCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAt                                    <  2:1655126/100‑1 (MQ=255)
                                                             ctgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtc                                  >  1:2888808/1‑100 (MQ=255)
                                                              tgctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATtct                                 <  1:4056732/100‑1 (MQ=255)
                                                                ctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTctct                               >  2:1444239/1‑100 (MQ=255)
                                                                ctCAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCAGTctct                               >  2:2604711/1‑100 (MQ=255)
                                                                  cAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTAc                             >  1:1661084/1‑100 (MQ=255)
                                                                  cAAACCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTAc                             >  1:2378675/1‑100 (MQ=255)
                                                                   aaaCCGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACg                            <  2:2161730/100‑1 (MQ=255)
                                                                      ccGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCg                         >  1:593580/1‑100 (MQ=255)
                                                                      ccGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCg                         <  1:1877502/100‑1 (MQ=255)
                                                                      ccGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCg                         >  1:142069/1‑100 (MQ=255)
                                                                       cGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGTCCAGTCTGGTCGGCGTGTTGCTGCTGCGCTTTTTCATTCTCTACGCCgg                        <  1:3213151/100‑1 (MQ=255)
                                                                       cGTGGGTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCgg                        <  1:779114/100‑1 (MQ=255)
                                                                           ggTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAg                    <  1:3223844/100‑1 (MQ=255)
                                                                           ggTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAg                    >  1:237860/1‑100 (MQ=255)
                                                                            gTCAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGt                   <  2:834759/100‑1 (MQ=255)
                                                                              cAATCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTg                 <  1:1342516/100‑1 (MQ=255)
                                                                                aTCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc               >  1:3985493/1‑100 (MQ=255)
                                                                                aTCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc               >  2:355440/1‑100 (MQ=255)
                                                                                aTCGCAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGAc               >  2:3946787/1‑100 (MQ=255)
                                                                                   gCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGt            >  2:1593730/1‑100 (MQ=255)
                                                                                    cAGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTg           <  1:3389373/100‑1 (MQ=255)
                                                                                     aGTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTgg          <  1:3150124/100‑1 (MQ=255)
                                                                                      gTTCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGc         >  1:1637781/1‑100 (MQ=255)
                                                                                       ttCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCg        <  1:97914/100‑1 (MQ=255)
                                                                                        tCCGGCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGt       <  1:99989/100‑1 (MQ=255)
                                                                                           ggCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAg    >  1:1172792/1‑100 (MQ=255)
                                                                                            gCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGc   <  1:1620920/100‑1 (MQ=255)
                                                                                            gCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGc   >  2:1708917/1‑100 (MQ=255)
                                                                                            gCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGc   >  2:3870584/1‑100 (MQ=255)
                                                                                            gCATGCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGc   <  1:3455528/100‑1 (MQ=255)
                                                                                                |                                                                                                
CGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAATGCTGCTCAAACCGTGGGTCAATCGCAGTTCCGGCATTCCTGCCGTGCTGGCGGCGTGTGGGGCCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCC  >  NC_000913/4291243‑4291435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: