BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,385,478	A→C	E29A (GAG→GCG)	yjeN →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,385,478	0	A	C	92.9%	537.4 / 15.5	170	E29A (GAG→GCG)	yjeN	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (6/6); new base C (66/92); total (72/98)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

ACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGT  >  NC_000913/4385381‑4385572

aCAGGAGATGTTATGGATGACACCAGCTGGGACCCGGACATAACAGAAGGTGAAATCAGGACGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAgcgc                                                                                              <  2:319218/100‑1 (MQ=255)

aCAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAgcgc                                                                                              <  2:2379767/100‑1 (MQ=255)

 cAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCa                                                                                             >  1:104034/1‑100 (MQ=255)

 cAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCa                                                                                             >  2:3058846/1‑100 (MQ=255)

 cAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCa                                                                                             >  1:3563995/1‑100 (MQ=255)

  aGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAg                                                                                            <  1:2077882/100‑1 (MQ=255)

   ggAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGa                                                                                           >  1:2596543/1‑100 (MQ=255)

    gAAACGTTTTGGATGGCACAAGACGGGACCTGGCAATAACCGAAGATGAAATAAGGGCGATGAAATTTTTTGTTGGGGATGTAGTGGAAATAGAGCTGag                                                                                          >  2:2002026/1‑99 (MQ=255)

     aaaTGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACg                                                                                         >  1:884815/1‑100 (MQ=255)

      aaTGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGa                                                                                        >  1:3204209/1‑100 (MQ=255)

         gTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtc                                                                                     <  2:972239/100‑1 (MQ=255)

         gTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtc                                                                                     <  1:3057891/100‑1 (MQ=255)

            aTGGATGACACCAGCCGGGACCCGGCAATAGCCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtctct                                                                                  <  1:3995283/100‑1 (MQ=255)

             tGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtctctc                                                                                 >  2:3048595/1‑100 (MQ=255)

             tGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtctctc                                                                                 <  2:896252/100‑1 (MQ=255)

             tGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATtctctc                                                                                 >  1:3339105/1‑100 (MQ=255)

              ggATGACACCAGCCGGGACCCGGCAATAACCGAAGATGCAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTctctct                                                                                >  2:3230607/1‑100 (MQ=255)

               gATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCtt                                                                               <  1:3046614/100‑1 (MQ=255)

                aTGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCttt                                                                              >  1:3638617/1‑100 (MQ=255)

                  gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTg                                                                            <  2:2486126/100‑1 (MQ=255)

                  gACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTg                                                                            <  2:1643758/100‑1 (MQ=255)

                   acacCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGt                                                                           >  2:3867347/1‑100 (MQ=255)

                    cacCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTc                                                                          <  2:3578822/100‑1 (MQ=255)

                     acgaGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCg                                                                         <  2:499293/97‑1 (MQ=255)

                     acCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCg                                                                         <  1:391088/100‑1 (MQ=255)

                     acCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCg                                                                         <  1:212281/100‑1 (MQ=255)

                     acCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCg                                                                         >  2:2780721/1‑100 (MQ=255)

                      ccAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCgc                                                                        >  2:1910911/1‑99 (MQ=255)

                       cAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAt                                                                       <  1:3403125/100‑1 (MQ=255)

                       cAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGCAAATAGCGCAGACGATTCTCTCTTTTGTCGAt                                                                       <  1:3933289/100‑1 (MQ=255)

                        aGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtg                                                                      >  2:1495736/1‑100 (MQ=255)

                        aGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtg                                                                      >  2:1495204/1‑100 (MQ=255)

                        aGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtg                                                                      <  1:2301279/100‑1 (MQ=255)

                         gCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgc                                                                     >  2:1178860/1‑100 (MQ=255)

                         gCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgc                                                                     >  1:759771/1‑100 (MQ=255)

                           cGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgcct                                                                   >  1:3104385/1‑100 (MQ=255)

                            gggACCCGGCAATAACCGAAGATGAAATCAGGGCTCTGCAATTTTCTGCCGGGGATGTAGCGGAAATATAGCAGACGATTCTCTCTTTTGTCGAtgcctg                                                                  <  2:1382595/100‑1 (MQ=255)

                            gggACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgcctg                                                                  <  1:1746073/100‑1 (MQ=255)

                            ggaaCCCGGCAATAACCGAAGATGGAATCAGGTCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgcctg                                                                  <  2:3311117/97‑1 (MQ=255)

                             ggACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgcctgc                                                                 <  1:917113/100‑1 (MQ=255)

                              gACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTTGAtgcctgcc                                                                <  2:3680878/100‑1 (MQ=255)

                              gACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGAtgcctgcc                                                                <  1:783067/100‑1 (MQ=255)

                               aCCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCca                                                               <  2:1804024/100‑1 (MQ=255)

                                cccGGCAATAACCGAAGATGAAATCAGGGCGCTGTAATTTTCTGCCGGGGATGCAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCcac                                                              >  2:3686285/1‑100 (MQ=255)

                                cccGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCcac                                                              <  2:3572991/100‑1 (MQ=255)

                                cccGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCcac                                                              >  1:55385/1‑100 (MQ=255)

                                cccGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCcac                                                              >  1:3536934/1‑100 (MQ=255)

                                cccGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGAAGACGATTCTCTCTTTTGTCGATGCCTGCcac                                                              >  2:3999940/1‑100 (MQ=255)

                                   ggCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACAcc                                                           <  1:2723791/100‑1 (MQ=255)

                                   ggCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACAcc                                                           >  1:3155231/1‑100 (MQ=255)

                                   ggCAATAACCGAAGAGGAAATCAGGGCGCTACAATTTTCTGCCGGAGATGTAGCGGAAATAGCGCAGACGATACTCTCTTTTGTCGATGCCTGCCACAcc                                                           >  1:3544745/1‑100 (MQ=255)

                                    gCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACAccc                                                          <  1:2149723/100‑1 (MQ=255)

                                      aaTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGt                                                        >  2:2027723/1‑100 (MQ=255)

                                         aaCCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTaaa                                                     >  2:1323867/1‑100 (MQ=255)

                                          aCCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAg                                                    >  1:2459149/1‑100 (MQ=255)

                                          aCCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAg                                                    <  2:1798467/100‑1 (MQ=255)

                                           ccGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGt                                                   <  2:2016879/100‑1 (MQ=255)

                                              aaGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGc                                                <  1:4030259/100‑1 (MQ=255)

                                               aGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCg                                               <  2:3948305/100‑1 (MQ=255)

                                                gATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGa                                              >  1:13713/1‑100 (MQ=255)

                                                 aTGAAATGAGGGCGCGGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAt                                             <  2:3303428/100‑1 (MQ=255)

                                                    aaaTCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggt                                          >  1:1931438/1‑100 (MQ=255)

                                                      aTCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtgg                                        >  1:2600085/1‑100 (MQ=255)

                                                       tCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggt                                       <  2:43566/100‑1 (MQ=255)

                                                       tCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggt                                       <  1:4013346/100‑1 (MQ=255)

                                                        cAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggtg                                      <  2:743867/100‑1 (MQ=255)

                                                         aGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggtgg                                     <  2:2694176/100‑1 (MQ=255)

                                                         aGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGAtggtggtgg                                     >  2:808339/1‑100 (MQ=255)

                                                          gggCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                    >  2:603592/1‑100 (MQ=255)

                                                          gggCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                    >  1:3683192/1‑100 (MQ=255)

                                                          gggCGCTGCAATTTTCTGCCGGGGATGCAGCGGACATAGCCGAGGCGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTggg                                    <  2:3817725/100‑1 (MQ=255)

                                                             cgcTGCAATTTTCTGCCGGGGATGTAGCGGAAATATCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAg                                 <  2:3938094/100‑1 (MQ=255)

                                                              gcTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGc                                <  2:3117566/100‑1 (MQ=255)

                                                              gcTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGc                                <  1:2493534/100‑1 (MQ=255)

                                                              gcTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGc                                <  1:1564829/100‑1 (MQ=255)

                                                               ntGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCa                               >  1:117971/2‑100 (MQ=255)

                                                                tGCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCAc                              >  1:1797970/1‑100 (MQ=255)

                                                                 gCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCAcc                             <  1:3881232/100‑1 (MQ=255)

                                                                 gCAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCAcc                             >  1:2813324/1‑100 (MQ=255)

                                                                  cAATTTTCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTCGCGATGGTGGTGGGTAGCACCa                            <  1:3417012/100‑1 (MQ=255)

                                                                   aaTTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCAt                           <  2:2970161/100‑1 (MQ=255)

                                                                     ttttCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTa                         >  1:1624716/1‑100 (MQ=255)

                                                                      tttCTGCCGGGGATGTAGCGGAAATAGCGCAGCCGATTCTCTCTCTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTaa                        <  1:3744519/100‑1 (MQ=255)

                                                                      tttCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTaa                        <  2:3468062/100‑1 (MQ=255)

                                                                      tttCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTaa                        >  2:2549515/1‑100 (MQ=255)

                                                                        tCTGCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAaca                      <  2:2929586/100‑1 (MQ=255)

                                                                           gCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAccc                   <  2:2717473/100‑1 (MQ=255)

                                                                           gCCGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACAccc                   >  1:3508401/1‑100 (MQ=255)

                                                                             cGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTc                 >  1:3763977/1‑100 (MQ=255)

                                                                             cGGGGATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTc                 >  1:1012356/1‑100 (MQ=255)

                                                                              ggtgATGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTAGATGCCTGCCACTCCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCa                <  2:3908649/97‑1 (MQ=255)

                                                                                 gATGGAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAg             <  2:2183844/100‑1 (MQ=255)

                                                                                  aTGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGa            >  2:3341572/1‑100 (MQ=255)

                                                                                  aTGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCAACACCAGTAGCGTGGGGAAGATGGTGGGTAGCACCATCAACACCATCACAGa            >  1:4078811/1‑100 (MQ=255)

                                                                                   tGTAGCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGAt           >  1:3132451/1‑100 (MQ=255)

                                                                                      aGCGGAAATAGCGCAGACGATTCTCTCCTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATcgc        <  1:796988/100‑1 (MQ=255)

                                                                                       gCGGAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATcgcg       <  1:2059836/100‑1 (MQ=255)

                                                                                          gAAATAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATg    <  1:2306227/100‑1 (MQ=255)

                                                                                          gAAATAGCGCAGACAATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATg    <  1:2001542/100‑1 (MQ=255)

                                                                                            aaTAGCGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGt  <  2:1744653/100‑1 (MQ=255)

ACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTGCCACACCCGTAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGT  >  NC_000913/4385381‑4385572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`