Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,452,800 T→G I77S (ATC→AGC)  ytfT → putative sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,452,8000TG93.9% 466.4 / 12.0 148I77S (ATC→AGC) ytfTputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base G (70/69);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGA  >  NC_000913/4452703‑4452895
                                                                                                 |                                                                                               
gtggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCg                                                                                               <  2:2651898/100‑1 (MQ=255)
 tggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAgcgc                                                                                              >  1:2535371/1‑100 (MQ=255)
 tggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAgcgc                                                                                              >  1:1849304/1‑100 (MQ=255)
  ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGcc                                                                                             >  1:776705/1‑100 (MQ=255)
   gtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCa                                                                                            >  2:8296/1‑100 (MQ=255)
   gtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCa                                                                                            >  1:1423949/1‑100 (MQ=255)
    tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCAc                                                                                           >  1:2779852/1‑100 (MQ=255)
    tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCAc                                                                                           >  1:4069736/1‑100 (MQ=255)
    tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCAc                                                                                           >  1:1718156/1‑100 (MQ=255)
         cAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTg                                                                                      <  2:619424/100‑1 (MQ=255)
           ggATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTggg                                                                                    >  1:1790451/1‑100 (MQ=255)
            gATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGa                                                                                   <  2:1481351/100‑1 (MQ=255)
            gATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGa                                                                                   >  1:526121/1‑100 (MQ=255)
                ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGa                                                                               >  2:1260084/1‑100 (MQ=255)
                ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGa                                                                               >  1:2320613/1‑100 (MQ=255)
                 gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGAt                                                                              <  2:738274/100‑1 (MQ=255)
                 gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGAt                                                                              >  2:1531911/1‑100 (MQ=255)
                  cGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGAtc                                                                             <  1:2483761/100‑1 (MQ=255)
                  cGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGAtc                                                                             <  1:479422/100‑1 (MQ=255)
                     ttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGAtctct                                                                          >  1:3009706/1‑100 (MQ=255)
                       gTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTcc                                                                        <  2:3452844/100‑1 (MQ=255)
                       gTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTcc                                                                        <  1:645669/100‑1 (MQ=255)
                       gTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTcc                                                                        >  1:245718/1‑100 (MQ=255)
                         tCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGt                                                                      >  2:2006704/1‑100 (MQ=255)
                           ggTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAg                                                                    >  1:2589666/1‑100 (MQ=255)
                            gTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAgg                                                                   <  1:2164368/100‑1 (MQ=255)
                            gTAGCCCCATAGACATTCTTAACCGTGCAGCGCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAgg                                                                   <  2:1677982/100‑1 (MQ=255)
                               gCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGc                                                                >  1:2103422/1‑100 (MQ=255)
                                 cccATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCgg                                                              <  2:2737732/100‑1 (MQ=255)
                                 ccaaTAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACACTGGTAAGCGAAACCGGTGGGCTTGATATCTCCGTCGGGGAgg                                                              >  2:984704/4‑100 (MQ=255)
                                    aTAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa                                                           >  2:2947546/1‑100 (MQ=255)
                                    aTAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa                                                           >  2:3182669/1‑100 (MQ=255)
                                     tAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt                                                          <  2:2797392/100‑1 (MQ=255)
                                     tAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt                                                          <  2:1377830/100‑1 (MQ=255)
                                     tAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt                                                          <  2:2797923/100‑1 (MQ=255)
                                     tAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt                                                          <  2:345335/100‑1 (MQ=255)
                                      aGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg                                                         <  1:377314/100‑1 (MQ=255)
                                      aGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg                                                         >  1:3947199/1‑100 (MQ=255)
                                      aGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg                                                         >  2:1210259/1‑100 (MQ=255)
                                      aGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg                                                         <  2:3794901/100‑1 (MQ=255)
                                       gACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATgg                                                        >  1:1474663/1‑100 (MQ=255)
                                       gACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATgg                                                        <  2:2927151/100‑1 (MQ=255)
                                       gACACTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTTATgg                                                        <  1:2873730/100‑1 (MQ=255)
                                        aCATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGc                                                       <  1:3523253/100‑1 (MQ=255)
                                         cATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCt                                                      >  2:2137461/1‑100 (MQ=255)
                                            tCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGCTGGCTATc                                                   >  2:3635758/1‑100 (MQ=255)
                                            tCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATc                                                   <  1:1675466/100‑1 (MQ=255)
                                            tCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATc                                                   >  1:1521461/1‑100 (MQ=255)
                                             cTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCg                                                  <  1:2528532/100‑1 (MQ=255)
                                               tAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGcc                                                <  1:1751500/100‑1 (MQ=255)
                                               tAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGcc                                                >  1:398911/1‑100 (MQ=255)
                                                 aCCGTGCAGCTCCCGTGGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCgg                                              >  2:702375/1‑100 (MQ=255)
                                                  ccGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCggn                                             <  2:1514969/100‑2 (MQ=255)
                                                  ccGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGa                                             >  1:2645951/1‑100 (MQ=255)
                                                  ccGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGa                                             >  2:776836/1‑100 (MQ=255)
                                                  ccGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGCGATAGCCATCACCgcc                                             <  1:1044511/100‑3 (MQ=255)
                                                   cGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAg                                            >  2:2263456/1‑100 (MQ=255)
                                                   cGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAg                                            <  1:3266628/100‑1 (MQ=255)
                                                   cGTGCAGCTCCCGTTGCGTCACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATTTCACCGTAGGGGCGGTGATGGCTATCGCCGGAg                                            >  2:3312368/1‑100 (MQ=255)
                                                    gTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGc                                           <  1:1399746/100‑1 (MQ=255)
                                                    gTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGc                                           <  1:297663/100‑1 (MQ=255)
                                                     tGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGGTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGcc                                          <  1:2030118/100‑1 (MQ=255)
                                                     tGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGcc                                          >  2:1132108/1‑100 (MQ=255)
                                                     tGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGcc                                          <  2:2550081/100‑1 (MQ=255)
                                                      gCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCca                                         <  1:3381775/100‑1 (MQ=255)
                                                      gCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCca                                         >  1:887112/1‑100 (MQ=255)
                                                      gCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCca                                         >  1:3962289/1‑100 (MQ=255)
                                                        aGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCcaca                                       <  1:8398/100‑1 (MQ=255)
                                                         gCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACaa                                      >  1:3990101/1‑100 (MQ=255)
                                                          cTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAAc                                     >  1:77406/1‑100 (MQ=255)
                                                              cGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCt                                 >  1:13671/1‑100 (MQ=255)
                                                                ttGCGTTACGGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGc                               <  2:1974850/100‑1 (MQ=255)
                                                                   cGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGTCAGTGATGGCTATCGCCGGAGCCACAACGGCTGCGAt                            <  2:3907370/100‑1 (MQ=255)
                                                                   cGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGAt                            <  1:3625935/100‑1 (MQ=255)
                                                                   cGTTACTGGCGATGGGCATGACGCTGGTGAGCGCCTTCAGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGAt                            <  2:1879996/100‑1 (MQ=255)
                                                                    gTTACTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATg                           >  2:1582675/1‑100 (MQ=255)
                                                                       aCTGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACg                        <  1:1201504/100‑1 (MQ=255)
                                                                         tGGCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGt                      >  2:2577556/1‑100 (MQ=255)
                                                                         tGGCGATTGGCATGACGCTGGTGAGCGCCACAGGTGGGATTGATCTCTCCGTAGGGCCGGTGATGGCTATCGCTGGAGCCACCACGGCTGCGCTGACGGt                      >  2:3036256/1‑100 (MQ=255)
                                                                          ggCGATTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTc                     >  1:506529/1‑100 (MQ=255)
                                                                             gATTGGCCTGACGCTGGAGAGCGCCACCGGTGCGATTGATCTCTACGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTcgcg                  <  1:3744943/100‑1 (MQ=255)
                                                                              atgggCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCgg                 <  2:2050828/97‑1 (MQ=255)
                                                                              aTTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCgg                 <  1:605368/100‑1 (MQ=255)
                                                                              aTTGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCgg                 <  2:3121031/100‑1 (MQ=255)
                                                                              aTTGGCATGACGCTGGGGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATCGCTGTCGCCGGAGCCACAACGGCTGCGATGACGGTCGCgg                 <  2:3755875/100‑1 (MQ=255)
                                                                               tttgCATGACGCTGGTGAGCGCCACCGTTGGGATTGATCTCTCCGTAGGGTCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCggg                <  2:3125517/97‑1 (MQ=255)
                                                                                tGGCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGa               >  1:1560350/1‑100 (MQ=255)
                                                                                 ggCATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCCCGGGAt              >  2:1389647/1‑100 (MQ=255)
                                                                                  gCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGAtt             >  2:1920980/1‑100 (MQ=255)
                                                                                   cATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTc            <  1:2727907/100‑1 (MQ=255)
                                                                                   cATGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTc            >  2:1279946/1‑100 (MQ=255)
                                                                                    aTGACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCa           >  2:3238489/1‑100 (MQ=255)
                                                                                      gACGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGc         >  1:1905318/1‑100 (MQ=255)
                                                                                       aCGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGcc        >  2:3549376/1‑100 (MQ=255)
                                                                                        cGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCt       >  2:1451392/1‑100 (MQ=255)
                                                                                        cGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCt       >  1:227814/1‑100 (MQ=255)
                                                                                        cGCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCt       >  1:1640682/1‑100 (MQ=255)
                                                                                         gCTGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTg      <  2:56117/100‑1 (MQ=255)
                                                                                           tGGTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGcc    <  1:3409690/100‑1 (MQ=255)
                                                                                             gTGAGCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTCTCGCCGGAGCCACAACGGCTGCGATGACGGTCGCCGGATTCAGCCTGCCGa  >  2:2498650/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGA  >  NC_000913/4452703‑4452895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: