Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,464,914 T→G I423L (ATC→CTC)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,464,9140TG93.9% 502.9 / 11.6 163I423L (ATC→CTC) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base G (72/81);  total (77/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

GTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGAT  >  NC_000913/4464815‑4465010
                                                                                                   |                                                                                                
gTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAt                                                                                                  <  1:1415088/100‑1 (MQ=255)
  aCAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGc                                                                                                <  1:1756884/100‑1 (MQ=255)
   cAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGcc                                                                                               >  1:2867492/1‑100 (MQ=255)
     aTCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCAt                                                                                             >  2:1443934/1‑100 (MQ=255)
     aTCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCAt                                                                                             >  2:1763393/1‑100 (MQ=255)
       cGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTc                                                                                           >  1:3489482/1‑100 (MQ=255)
        gggATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCg                                                                                          <  2:1785761/100‑1 (MQ=255)
         ggATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGc                                                                                         <  1:3524954/100‑1 (MQ=255)
            tgatggtgGCGGTAGCCACTGCCAGCGCAAACAGCTGCCAGTAGCTCGGTTGTATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCGAt                                                                                      <  2:3997489/100‑1 (MQ=255)
             gatgatgTCGATAGCCACTGCCAGCGCAAACAGCTGCCAGTAGCGCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATa                                                                                     <  1:3744092/100‑1 (MQ=255)
              atgatgGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATaa                                                                                    >  2:176414/1‑100 (MQ=255)
               tgatgGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAAc                                                                                   <  2:1877024/100‑1 (MQ=255)
                gatgGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACg                                                                                  >  2:213321/1‑100 (MQ=255)
                gatgGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACg                                                                                  >  2:2808608/1‑100 (MQ=255)
                  tgGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGcc                                                                                >  2:3272703/1‑100 (MQ=255)
                   ggCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCg                                                                               >  1:2082348/1‑100 (MQ=255)
                   ggCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCg                                                                               <  2:3971014/100‑1 (MQ=255)
                    gggATAGCCGTTGCCTGCGCAATCACCTTCCCGTCGCTCGGTGGGATCGAGAGGATTCCCGGCAGGCCGCCTCGGCCGAGGCCGCTCGCCGTAACTCCGt                                                                              <  2:2943785/98‑1 (MQ=255)
                          gCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGc                                                                        <  1:3306136/100‑1 (MQ=255)
                          gCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGc                                                                        >  1:843267/1‑100 (MQ=255)
                          gCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGc                                                                        >  2:352516/1‑100 (MQ=255)
                          gCCAATGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCACCATAACGCCGTTCAGGc                                                                        >  2:3386789/1‑100 (MQ=255)
                             aTTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGc                                                                     >  2:215944/1‑100 (MQ=255)
                             aTTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGc                                                                     <  1:898971/100‑1 (MQ=255)
                              ttGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCa                                                                    <  2:268926/100‑1 (MQ=255)
                               tGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGACTCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATCACGCCGTTCAGGCCGCAt                                                                   >  2:3696318/1‑100 (MQ=255)
                                 ccAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAg                                                                 >  1:3161557/1‑100 (MQ=255)
                                 ccAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAg                                                                 <  1:3407230/100‑1 (MQ=255)
                                  cAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGc                                                                <  1:4032270/100‑1 (MQ=255)
                                  cAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGc                                                                <  1:323414/100‑1 (MQ=255)
                                  cAGCGCAAACACCCGCCAGTAGATCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGc                                                                <  1:393445/100‑1 (MQ=255)
                                     cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAAt                                                             <  1:1397227/100‑1 (MQ=255)
                                      gcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATc                                                            <  1:3092734/100‑1 (MQ=255)
                                      gcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATc                                                            <  2:748642/100‑1 (MQ=255)
                                       cAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATcc                                                           >  2:2525377/1‑100 (MQ=255)
                                       cAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATcc                                                           >  1:135472/1‑100 (MQ=255)
                                       aatacatCTGCCAGTAGCTCGGTTGAAACAAGATAATTCCCGGCAATCCTGCTACGCCGATGCCATTCGGCATATCGTCGTGTAGGCTGCCTATCAATca                                                           >  2:3474265/4‑99 (MQ=255)
                                         aaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTGCGCCGCGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTg                                                         <  2:3259029/100‑1 (MQ=255)
                                         aaCACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTg                                                         <  2:2187879/100‑1 (MQ=255)
                                          acacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGc                                                        <  1:68143/100‑1 (MQ=255)
                                          acacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGc                                                        >  2:1254359/1‑100 (MQ=255)
                                           cacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGcc                                                       <  1:3656036/100‑1 (MQ=255)
                                           cacCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGcc                                                       <  2:895360/100‑1 (MQ=255)
                                            acCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGcca                                                      <  2:1631141/100‑1 (MQ=255)
                                             ccTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccag                                                     <  1:3861348/100‑1 (MQ=255)
                                             ccTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccag                                                     >  1:2797344/1‑100 (MQ=255)
                                               tGCCAGTAGCTCGGTTGCATCGAGCGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagac                                                   <  2:2141085/100‑1 (MQ=255)
                                                gCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagacc                                                  <  1:3456450/100‑1 (MQ=255)
                                                 ccAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagacca                                                 <  1:1506873/100‑1 (MQ=255)
                                                   aGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagaccaga                                               >  1:371804/1‑100 (MQ=255)
                                                   aGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagaccaga                                               >  1:659716/1‑100 (MQ=255)
                                                   aGTAGCTCGGTTGAATCGAGAGAATCCCCGGCAGGCCTCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagaccaga                                               <  1:3800168/100‑1 (MQ=255)
                                                     tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc                                             <  1:3682345/100‑1 (MQ=255)
                                                     tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc                                             <  2:2430568/100‑1 (MQ=255)
                                                     tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc                                             <  1:253195/100‑1 (MQ=255)
                                                     tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc                                             <  1:161888/100‑1 (MQ=255)
                                                     tAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc                                             <  1:1514899/100‑1 (MQ=255)
                                                      aGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAcc                                            <  2:785397/100‑1 (MQ=255)
                                                       gCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCCTAGCAACCCTGCCAGACCAGAACCa                                           >  2:1277628/1‑100 (MQ=255)
                                                       gCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCa                                           <  1:1927618/100‑1 (MQ=255)
                                                          cGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAatc                                        >  2:1059856/1‑100 (MQ=255)
                                                           gggtGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAatca                                       <  1:963025/97‑1 (MQ=255)
                                                            gTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAatcat                                      >  1:2372421/1‑100 (MQ=255)
                                                             ttGAATCGAGAGCATTCCCGGCAGGCCGCCGACGCCGAGGCCATTCGCTATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAatcatc                                     <  1:3887001/100‑1 (MQ=255)
                                                              tGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCACATAGCAATCCTGCCAGACCAGAACCAATCATcg                                    <  1:763146/100‑1 (MQ=255)
                                                                aaTCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATcgcg                                  >  2:3579395/1‑100 (MQ=255)
                                                                 aTCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCgcgc                                 >  1:3958276/1‑100 (MQ=255)
                                                                  tCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca                                <  1:3947143/100‑1 (MQ=255)
                                                                    gagagaATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGcaca                              >  2:3775500/1‑100 (MQ=255)
                                                                     agagaATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAg                             <  1:2547409/100‑1 (MQ=255)
                                                                      gagaATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGc                            <  2:3840960/100‑1 (MQ=255)
                                                                        gaATTCCCGGCAGGCCGCCTACGCCGAGGCCATTCACCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCAt                          >  2:157571/1‑100 (MQ=255)
                                                                          atccccGGCAGGCCGCCTACCCCGAGGCCAGTCACCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCg                        <  2:1640064/97‑1 (MQ=255)
                                                                           ttCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCgg                       <  1:1248973/100‑1 (MQ=255)
                                                                           ttCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCgg                       >  1:2322467/1‑100 (MQ=255)
                                                                           ttCCCGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCgg                       <  2:1988436/100‑1 (MQ=255)
                                                                             cccGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGa                     >  2:1044136/1‑100 (MQ=255)
                                                                             cccGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGa                     >  1:2605857/1‑100 (MQ=255)
                                                                             cccGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGa                     >  2:2320795/1‑100 (MQ=255)
                                                                             cccGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGa                     <  1:1995409/100‑1 (MQ=255)
                                                                               cGGCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAg                   >  2:544068/1‑100 (MQ=255)
                                                                                ggCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc                  <  2:3771662/100‑1 (MQ=255)
                                                                                ggCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc                  <  1:3126719/100‑1 (MQ=255)
                                                                                ggCAGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc                  <  2:3368293/100‑1 (MQ=255)
                                                                                ggCAGGCCGCCGACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCGGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc                  <  2:1819695/100‑1 (MQ=255)
                                                                                   aGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGat               >  2:2354977/1‑100 (MQ=255)
                                                                                   aGGCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGat               <  1:1231564/100‑1 (MQ=255)
                                                                                   aGGCCGCCTACACCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGat               >  1:1594870/1‑100 (MQ=255)
                                                                                    ggCCGCCTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGata              >  1:3337707/1‑100 (MQ=255)
                                                                                      ccgccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt            <  2:3559494/100‑1 (MQ=255)
                                                                                      ccgccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt            >  1:2637937/1‑100 (MQ=255)
                                                                                      ccgccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt            >  2:2864171/1‑100 (MQ=255)
                                                                                      ccgccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt            <  2:2597708/100‑1 (MQ=255)
                                                                                      ccgccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt            >  1:225784/1‑100 (MQ=255)
                                                                                         ccTACGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCa         <  1:2038221/100‑1 (MQ=255)
                                                                                            aCGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt      <  2:2497443/100‑1 (MQ=255)
                                                                                            aCGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt      <  2:2339888/100‑1 (MQ=255)
                                                                                             cGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt     <  1:2270829/100‑1 (MQ=255)
                                                                                             cGCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt     >  2:2207499/1‑100 (MQ=255)
                                                                                              gCCGAGGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTg    <  2:2508046/100‑1 (MQ=255)
                                                                                                   |                                                                                                
GTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGAT  >  NC_000913/4464815‑4465010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: