Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,499,550 A→C intergenic (+50/+43) insD1 → / ← yjgX IS2 transposase TnpB/pseudogene fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,499,5500AC97.7% 451.6 / ‑2.1 132intergenic (+50/+43)insD1/yjgXIS2 transposase TnpB/pseudogene fragment
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (60/69);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 132 total aligned reads displayed.

CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTAT  >  NC_000913/4499453‑4499646
                                                                                                 |                                                                                                
cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCACATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCt                                                                                                >  2:2587008/1‑100 (MQ=255)
cAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCt                                                                                                <  1:275812/100‑1 (MQ=255)
cAGCGGGCTTGTAATGGGTTAACTGATACCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCt                                                                                                <  1:3713695/100‑1 (MQ=255)
    gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTc                                                                                            <  1:3068891/100‑1 (MQ=255)
    gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTc                                                                                            <  1:1649799/100‑1 (MQ=255)
     ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCa                                                                                           >  1:275693/1‑100 (MQ=255)
      gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAg                                                                                          <  2:1089464/100‑1 (MQ=255)
      gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAg                                                                                          <  2:3111957/100‑1 (MQ=255)
      gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAg                                                                                          <  1:3348914/100‑1 (MQ=255)
      gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAg                                                                                          >  2:3433574/1‑100 (MQ=255)
       cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGt                                                                                         >  2:3044141/1‑100 (MQ=255)
       cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGt                                                                                         >  1:673234/1‑100 (MQ=255)
        ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTa                                                                                        >  1:2145326/1‑100 (MQ=255)
        ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTa                                                                                        >  1:3193167/1‑100 (MQ=255)
         tGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTAc                                                                                       >  1:415914/1‑100 (MQ=255)
         atctaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTAc                                                                                       <  1:958130/96‑1 (MQ=255)
          gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACa                                                                                      <  2:2276280/100‑1 (MQ=255)
          gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACa                                                                                      >  2:1085795/1‑100 (MQ=255)
           tAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACaa                                                                                     >  1:1004426/1‑100 (MQ=255)
            aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAg                                                                                    <  1:3230411/100‑1 (MQ=255)
            aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAg                                                                                    >  1:1150135/1‑100 (MQ=255)
            aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAg                                                                                    <  1:2004853/100‑1 (MQ=255)
             aTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACagat                                                                                   >  2:938063/1‑97 (MQ=255)
               gggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGa                                                                                 >  2:2466758/1‑100 (MQ=255)
                ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGAc                                                                                >  2:1738360/1‑100 (MQ=255)
                   tAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGt                                                                             >  2:2863263/1‑100 (MQ=255)
                    aaGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTa                                                                            <  1:3171662/100‑1 (MQ=255)
                     aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTAt                                                                           <  1:2843538/100‑1 (MQ=255)
                      gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTAtt                                                                          >  2:1757896/1‑100 (MQ=255)
                        gATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACGGAATCCTTTTCAGTACAAGCGACTGTATTAc                                                                        <  1:3266149/100‑1 (MQ=255)
                        gATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTAc                                                                        >  1:2401354/1‑100 (MQ=255)
                        gATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTAc                                                                        <  1:3281073/100‑1 (MQ=255)
                        gATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTAc                                                                        <  1:429883/100‑1 (MQ=255)
                          tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAg                                                                      <  2:1698824/100‑1 (MQ=255)
                          tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAg                                                                      <  1:151770/100‑1 (MQ=255)
                          tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAg                                                                      <  1:460613/100‑1 (MQ=255)
                           aaCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGt                                                                     >  1:3910322/1‑100 (MQ=255)
                            aCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTc                                                                    >  2:2330122/1‑100 (MQ=255)
                             cAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCt                                                                   <  1:2956284/100‑1 (MQ=255)
                               gATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGc                                                                 <  2:1007733/100‑1 (MQ=255)
                               gATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTAATACAGTcgct                                                                 <  2:2384040/100‑4 (MQ=39)
                                aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGcc                                                                <  2:1922709/100‑1 (MQ=255)
                                 tGTCTGGAAATATAGGGGCAAATCCCCCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                                               <  2:3958871/100‑1 (MQ=255)
                                  gTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCat                                                              <  2:1555625/100‑1 (MQ=255)
                                  gTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCat                                                              >  1:944594/1‑100 (MQ=255)
                                    cttgAAATATAGGGGCAAATCCACCTTGTGGTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTGCAGTCTGCCatat                                                            <  2:1169605/97‑1 (MQ=255)
                                    cTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatat                                                            >  2:1430733/1‑100 (MQ=255)
                                     tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatata                                                           >  1:48892/1‑100 (MQ=255)
                                      ggAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                          >  2:919734/1‑100 (MQ=255)
                                       gAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATg                                                         <  1:697608/100‑1 (MQ=255)
                                       gAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATg                                                         >  1:880923/1‑100 (MQ=255)
                                        aaaTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGa                                                        >  1:30049/1‑100 (MQ=255)
                                        aaaTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGa                                                        <  2:2848001/100‑1 (MQ=255)
                                             taGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAcc                                                   <  1:1165849/100‑1 (MQ=255)
                                             taGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAcc                                                   <  2:2045316/100‑1 (MQ=255)
                                             taGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGAATAcc                                                   <  2:3377960/100‑1 (MQ=255)
                                               ggggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtg                                                 >  2:271968/1‑100 (MQ=255)
                                               ggggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtg                                                 <  1:837121/100‑1 (MQ=255)
                                                 ggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATAGATGATTACCtgtg                                               >  2:67229/1‑100 (MQ=255)
                                                     aaTCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCa                                           <  1:1392868/100‑1 (MQ=255)
                                                      aTCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAg                                          >  2:3563953/1‑100 (MQ=255)
                                                      aTCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAg                                          >  2:3745078/1‑100 (MQ=255)
                                                      aTCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGACTGCCATATATGATTACCTGTGATCAg                                          >  1:116342/1‑100 (MQ=255)
                                                         cACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                                       <  1:2625268/100‑1 (MQ=255)
                                                          aCCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                                      >  1:3848191/1‑100 (MQ=255)
                                                          aCCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                                      >  2:3199747/1‑100 (MQ=255)
                                                          aCCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                                      >  2:2215967/1‑100 (MQ=255)
                                                          aCCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                                      <  1:1767132/100‑1 (MQ=255)
                                                             ttGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                                   >  2:3526043/1‑100 (MQ=255)
                                                              tgtgCTGATACGAATACGGATATTTTATACAGAATACTTTTGAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATa                                  <  2:600836/100‑1 (MQ=255)
                                                                 gCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGt                               <  2:1447903/100‑1 (MQ=255)
                                                                   tGATATGATTACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTgg                             >  2:3840918/1‑100 (MQ=255)
                                                                    gATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGc                            <  2:1342316/100‑1 (MQ=255)
                                                                      tatGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTg                          >  2:1308654/1‑100 (MQ=255)
                                                                      tatGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTg                          >  1:1300909/1‑100 (MQ=255)
                                                                        tGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg                        >  2:911762/1‑100 (MQ=255)
                                                                        tGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg                        <  1:1694601/100‑1 (MQ=255)
                                                                         gAATACGGATATTTTATACAGCATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATACATGATTACATGTGATCAGGAAGAATAAGTGGCTGAgg                       <  2:2194497/100‑1 (MQ=255)
                                                                         gAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCTTATATGCTTACCTGTGATCAGGAAGAATAAGTGGCTGAgg                       >  2:3611805/1‑100 (MQ=255)
                                                                         gAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg                       <  2:3765895/100‑1 (MQ=255)
                                                                         gAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg                       >  1:1543988/1‑100 (MQ=255)
                                                                         gAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg                       >  2:1194975/1‑100 (MQ=255)
                                                                          aaTACGGATCTTTTATACAGAATCCTTTTCAGTACAGGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGt                      <  2:2193653/100‑1 (MQ=255)
                                                                          aaTACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGt                      <  2:1153606/100‑1 (MQ=255)
                                                                           aTACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGtt                     <  2:2783535/100‑1 (MQ=255)
                                                                             aCGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGtttn                   <  1:3250336/100‑2 (MQ=255)
                                                                              cGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCa                  >  1:192421/1‑100 (MQ=255)
                                                                               ggATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaa                 <  1:1377875/100‑1 (MQ=255)
                                                                                 atatTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAAc               <  1:1550804/100‑1 (MQ=255)
                                                                                   atTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTg             >  1:265029/1‑100 (MQ=255)
                                                                                   atTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTg             >  2:2256304/1‑100 (MQ=255)
                                                                                     tttATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTc           <  1:2007005/100‑1 (MQ=255)
                                                                                     tttATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTc           <  1:495273/100‑1 (MQ=255)
                                                                                     tttATACAGAATCCCTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTc           <  2:3660670/100‑1 (MQ=255)
                                                                                           cAGAATCCTTTTCAGTACAAGCGACTGTATTATAGTCTGCCATATATGATTAACAAAAAAAAAAAAAAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg     <  2:1979113/100‑1 (MQ=255)
                                                                                           cAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg     >  2:610697/1‑100 (MQ=255)
                                                                                           cAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg     <  2:47052/100‑1 (MQ=255)
                                                                                           cAGAATCCTTTTCAGTACAAGCGACCGTATTACAGTCTGCCATATATGATCACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg     <  2:677084/100‑1 (MQ=255)
                                                                                             gAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGta   <  1:2285822/100‑1 (MQ=255)
                                                                                              aaTCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGtat  >  1:2222353/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTAT  >  NC_000913/4499453‑4499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: