Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,532,668 T→G G172G (GGA→GGC yjhP ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,532,6680TG93.0% 522.0 / 17.2 173G172G (GGA→GGCyjhPputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (8/4);  new base G (80/80);  total (88/85)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

GGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGT  >  NC_000913/4532571‑4532764
                                                                                                 |                                                                                                
ggcgCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGcc                                                                                                <  2:2102246/100‑1 (MQ=255)
  cgcATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGa                                                                                              <  1:2739808/100‑1 (MQ=255)
      tGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAg                                                                                          >  2:1282342/1‑100 (MQ=255)
      tGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAg                                                                                          >  1:2205358/1‑100 (MQ=255)
         tCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTcc                                                                                       <  2:240749/100‑1 (MQ=255)
           aGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTg                                                                                     <  1:468324/100‑1 (MQ=255)
            gCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTgg                                                                                    <  2:2673951/100‑1 (MQ=255)
             ccATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGc                                                                                   <  1:2536179/100‑1 (MQ=255)
                   tGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCAGGCAGCGTc                                                                             >  2:3460826/1‑100 (MQ=255)
                     cGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAg                                                                           >  2:1359707/1‑100 (MQ=255)
                     cGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAg                                                                           <  1:834407/100‑1 (MQ=255)
                     cGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAg                                                                           >  2:1591845/1‑100 (MQ=255)
                      ggCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAgg                                                                          <  1:2661341/100‑1 (MQ=255)
                      ggCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAgg                                                                          <  1:2531814/100‑1 (MQ=255)
                           cATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAAtn                                                                     <  1:3257536/100‑2 (MQ=255)
                           cATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATc                                                                     <  2:687263/100‑1 (MQ=255)
                            aTACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCg                                                                    <  1:1870667/100‑1 (MQ=255)
                            aTACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCg                                                                    <  1:1870580/100‑1 (MQ=255)
                            aTACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCg                                                                    <  2:3250888/100‑1 (MQ=255)
                            aTACCGGTACCAGCCTTCCTGGTCTGCGAGTACTATTTCAACCACGTAATAACCGATATCGTAGAACGCGCCAACAAGTCCTGGCCGCGTCAGGAAATCg                                                                    >  2:2928416/1‑100 (MQ=255)
                             tACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGc                                                                   >  2:3749896/1‑100 (MQ=255)
                             tACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGc                                                                   <  1:1557107/100‑1 (MQ=255)
                               ccGGTCCCAGCCTTCCTGGTCTGCGAGTCCCCTTTCCACCACGCCATGGCCGCGATCGTCGAACGCGCGGACAAGTCCTGCCAGCGGCAGGAAATCGccg                                                                 >  2:2824783/1‑98 (MQ=255)
                                 ggTCCCAGCGTGCCTGGTCTGCCAGTACCATTTCCACCCCGTCATAGCCGAGATGGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGt                                                               <  2:3899502/100‑1 (MQ=255)
                                 ggTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGt                                                               >  1:1906040/1‑100 (MQ=255)
                                  gTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTc                                                              <  1:1352019/100‑1 (MQ=255)
                                   tCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCg                                                             >  1:1859734/1‑100 (MQ=255)
                                     ccAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAg                                                           <  2:1144436/100‑1 (MQ=255)
                                      cAGCCTTCCTGGTCTGCCAGTACCATTTCCATCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGACGAGc                                                          <  2:3523194/100‑1 (MQ=255)
                                      cAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGc                                                          <  1:2468433/100‑1 (MQ=255)
                                      cAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGc                                                          >  2:1705428/1‑100 (MQ=255)
                                      cAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAACGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCgcgc                                                          >  2:615555/1‑100 (MQ=255)
                                          cTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGAc                                                      <  1:1447248/100‑1 (MQ=255)
                                          cTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGAc                                                      >  2:301925/1‑100 (MQ=255)
                                            tCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGAcgc                                                    >  1:167123/1‑100 (MQ=255)
                                             ccTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGAcgcc                                                   <  1:1903603/100‑1 (MQ=255)
                                               tGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGCCAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGATGAcgccgc                                                 >  2:3751207/1‑100 (MQ=255)
                                               tGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGAcgccgc                                                 >  2:3028489/1‑100 (MQ=255)
                                                ggTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCa                                                >  2:3218986/1‑100 (MQ=255)
                                                ggTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCa                                                >  2:1676543/1‑100 (MQ=255)
                                                 gTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAg                                               >  1:845524/1‑100 (MQ=255)
                                                 gTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGACCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAg                                               >  1:3335600/1‑100 (MQ=255)
                                                  tCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAgg                                              <  1:2824551/100‑1 (MQ=255)
                                                   cTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGc                                             >  1:2568467/1‑100 (MQ=255)
                                                   cTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGc                                             <  1:3821133/100‑1 (MQ=255)
                                                     gCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCt                                           >  2:1910910/1‑100 (MQ=255)
                                                      ncAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTg                                          >  2:3984623/2‑100 (MQ=255)
                                                      ccAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTg                                          >  1:3172161/1‑100 (MQ=255)
                                                      ccAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTg                                          <  1:3467414/100‑1 (MQ=255)
                                                      ccAGTAACATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTg                                          <  2:2693968/100‑1 (MQ=255)
                                                        aGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTggg                                        <  2:2958704/100‑1 (MQ=255)
                                                        aGTACCATTTCCACCACGTCATAGCCGAGAACGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTggg                                        >  2:1472549/1‑100 (MQ=255)
                                                         gTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGc                                       <  2:1822493/100‑1 (MQ=255)
                                                         gTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGc                                       <  2:1829825/100‑1 (MQ=255)
                                                         gTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGc                                       >  1:2079886/1‑100 (MQ=255)
                                                          tACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCt                                      <  1:392478/100‑1 (MQ=255)
                                                           aCCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCCGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCtc                                     >  2:3146400/1‑99 (MQ=255)
                                                            ccATTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTAt                                    >  2:2302501/1‑100 (MQ=255)
                                                              aTTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTAttt                                  >  1:1032468/1‑100 (MQ=255)
                                                              aTTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTAttt                                  <  1:1153547/100‑1 (MQ=255)
                                                              aTTTCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTAttt                                  >  1:1196993/1‑100 (MQ=255)
                                                              aTTTCCACCAAGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTAttt                                  <  2:389752/100‑1 (MQ=255)
                                                               tttCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttc                                 >  2:2793892/1‑100 (MQ=255)
                                                               tttCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCGGGCAGCGTCAGGAAATCGGTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttc                                 <  1:3783334/100‑1 (MQ=255)
                                                                ttCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttct                                <  2:2888041/100‑1 (MQ=255)
                                                                 tCCACCACGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttctt                               >  1:2995488/1‑100 (MQ=255)
                                                                  ccaccaCGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttcttc                              <  2:1430263/100‑1 (MQ=255)
                                                                  ccaccaCGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATttcttc                              <  1:1974035/100‑1 (MQ=255)
                                                                   caccacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGTCGCCGAACGCCTGGGCTATTtcttct                             >  1:3819474/1‑100 (MQ=255)
                                                                   caccacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTtcttct                             <  1:704647/100‑1 (MQ=255)
                                                                   caccacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTtcttct                             <  1:1994401/100‑1 (MQ=255)
                                                                    accacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTg                            >  1:2999009/1‑100 (MQ=255)
                                                                     ccacGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGt                           >  2:2450426/1‑100 (MQ=255)
                                                                      cacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGtt                          >  1:2192982/1‑100 (MQ=255)
                                                                      cacGTCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGtt                          >  1:3740445/1‑100 (MQ=255)
                                                                       acGTCATAGCCGAGATCGTCGAACGCGCCGACTAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTg                         <  1:3738493/100‑1 (MQ=255)
                                                                         gTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCt                       >  2:2878287/1‑100 (MQ=255)
                                                                          tCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTg                      <  1:2252948/100‑1 (MQ=255)
                                                                          tCATAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTg                      <  1:3505423/100‑1 (MQ=255)
                                                                            aTAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGt                    <  1:306373/100‑1 (MQ=255)
                                                                            aTAGCCGAGATCGTCGAACGCGCCGACAACTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCTGCAGGCCTGTGCTATTTCTTCTGTTGCTGGt                    <  2:3807026/100‑1 (MQ=255)
                                                                             tAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTa                   <  1:648103/100‑1 (MQ=255)
                                                                             tAGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTa                   >  1:913855/1‑100 (MQ=255)
                                                                              aGCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAg                  >  1:3960611/1‑100 (MQ=255)
                                                                               gCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGc                 >  2:31698/1‑100 (MQ=255)
                                                                               gCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGc                 <  2:85667/100‑1 (MQ=255)
                                                                               gCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGc                 >  1:130721/1‑100 (MQ=255)
                                                                               gCCGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGc                 >  1:1564078/1‑100 (MQ=255)
                                                                                ccGAGATCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCt                >  2:623609/1‑100 (MQ=255)
                                                                                   agaTCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGAc             <  1:2890982/100‑1 (MQ=255)
                                                                                   agaTCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGAc             <  1:2718648/100‑1 (MQ=255)
                                                                                   agaTCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGAc             <  2:127291/100‑1 (MQ=255)
                                                                                    gaTCGTCGAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACg            >  1:466550/1‑100 (MQ=255)
                                                                                      tcgtcgAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGcc          <  2:3858936/100‑1 (MQ=255)
                                                                                       cgtcgAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCa         >  1:2110747/1‑100 (MQ=255)
                                                                                       cgtcgAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCa         >  1:1024118/1‑100 (MQ=255)
                                                                                        gtcgAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAg        <  1:1326201/100‑1 (MQ=255)
                                                                                          cgAACGCGCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGGCGAGCTGACGCCGCAGGACTGGGATATTTCTTCTGTTGCTGCTACCTGACGCCAGTa      >  2:1885336/1‑100 (MQ=255)
                                                                                             aCGCGCCTACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAggg   <  1:3751112/100‑1 (MQ=255)
                                                                                              cgcgCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGt  >  2:568436/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGCAGCGTCAGGAAATCGCTTGTCGAGCTGACGCCGCAGGCCTGGGCTATTTCTTCTGTTGCTGGTAGCTGACGCCAGTAGGGT  >  NC_000913/4532571‑4532764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: