BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	424,279	A→C	T588P (ACT→CCT)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	424,279	0	A	C	94.6%	408.3 / 9.2	130	T588P (ACT→CCT)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (61/61); total (64/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 134 total aligned reads displayed.

GTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCC  >  NC_000913/424180‑424371

    cAATCAACCGTGGCGAGGCTTGGGAAGTGGCGCTACCCGCGTCACTGTTTCTCAATGCCGTGCAATGCCACTGCAAAGAAGGGCATGGCCAActgcctga                                                                                          >  2:2716100/1‑100 (MQ=255)

    cAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAActgcctga                                                                                          >  1:736773/1‑100 (MQ=255)

     aaTCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACtgcctgac                                                                                         >  1:1826953/1‑100 (MQ=255)

      aTCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACg                                                                                        >  2:1885712/1‑100 (MQ=255)

       ccAACCGTGGCGAGGCCTGTGAAGGGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACgg                                                                                       <  1:3324328/99‑1 (MQ=255)

          aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGAt                                                                                    >  1:1121666/1‑100 (MQ=255)

          aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGAt                                                                                    >  2:1422202/1‑100 (MQ=255)

           ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGAtt                                                                                   >  2:3718860/1‑100 (MQ=255)

            cGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTc                                                                                  <  1:2301007/100‑1 (MQ=255)

                gCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGc                                                                              <  1:258716/100‑1 (MQ=255)

                gCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGc                                                                              >  1:1552475/1‑100 (MQ=255)

                  gAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCtt                                                                            <  1:3473739/100‑1 (MQ=255)

                  gAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCtt                                                                            <  2:1096389/100‑1 (MQ=255)

                    ggCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtg                                                                          >  2:2627835/1‑100 (MQ=255)

                      ccTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcc                                                                        >  1:820499/1‑100 (MQ=255)

                       cTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcct                                                                       >  1:2184034/1‑100 (MQ=255)

                        tgtgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcctg                                                                      <  1:4055852/100‑1 (MQ=255)

                          tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcctgcc                                                                    >  2:727329/1‑100 (MQ=255)

                          tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcctgcc                                                                    >  1:1820121/1‑100 (MQ=255)

                          tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGCGCATGGGCAACTGCCTGACGGGATTCTGGCTTtgcctgcc                                                                    <  1:1188212/100‑1 (MQ=255)

                            aaGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCAt                                                                  <  2:2368470/100‑1 (MQ=255)

                            aaGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCAt                                                                  >  2:3888146/1‑100 (MQ=255)

                              gtggtgCTACCCGCGTCACCGTTTCTCAATGCCATGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCAttt                                                                <  1:3779558/100‑1 (MQ=255)

                               tggtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTc                                                               <  1:909292/100‑1 (MQ=255)

                                ggtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCg                                                              <  2:1067300/100‑1 (MQ=255)

                                ggtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCg                                                              >  1:4063904/1‑100 (MQ=255)

                                 gtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCgg                                                             >  2:1098466/1‑100 (MQ=255)

                                  tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGc                                                            <  1:1080105/100‑1 (MQ=255)

                                  tgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTACCATTTCGGc                                                            <  1:3568586/100‑1 (MQ=255)

                                   gCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCt                                                           >  1:646186/1‑100 (MQ=255)

                                    cTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                                          <  1:1357974/100‑1 (MQ=255)

                                      aCCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                                        >  2:3124235/1‑100 (MQ=255)

                                       cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                       >  1:2870148/1‑100 (MQ=255)

                                       cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                       >  1:1401924/1‑100 (MQ=255)

                                       cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                       <  1:2783449/100‑1 (MQ=255)

                                       cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                       >  2:3269389/1‑100 (MQ=255)

                                       cccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                                       >  1:99302/1‑100 (MQ=255)

                                        ccGCGTCACCGTTTCTCAATGACGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgat                                                      >  2:1383340/1‑98 (MQ=255)

                                            gTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATgg                                                  <  2:2211484/100‑1 (MQ=255)

                                            gTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATgg                                                  >  1:2150302/1‑100 (MQ=255)

                                             tCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                 <  1:2364436/100‑1 (MQ=255)

                                             tCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                 <  1:3326853/100‑1 (MQ=255)

                                             tCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                 <  1:1926469/100‑1 (MQ=255)

                                             tCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                                 <  2:531797/100‑1 (MQ=255)

                                              cACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                                <  1:200168/100‑1 (MQ=255)

                                              cACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                                >  2:756355/1‑100 (MQ=255)

                                              cACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                                >  2:755581/1‑100 (MQ=255)

                                               aCCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATg                                               <  2:488944/100‑1 (MQ=255)

                                               aCCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATg                                               >  1:3491045/1‑100 (MQ=255)

                                               aCCGTTTCTCAATGCCGGGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATg                                               <  1:3066678/100‑1 (MQ=255)

                                                 cGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGaa                                             >  1:1037306/1‑100 (MQ=255)

                                                 cGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGaa                                             >  1:127771/1‑100 (MQ=255)

                                                  gTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                                            <  2:338179/100‑1 (MQ=255)

                                                  gTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                                            <  2:1075895/100‑1 (MQ=255)

                                                  gTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                                            <  1:12515/100‑1 (MQ=255)

                                                  gTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                                            <  2:3160906/100‑1 (MQ=255)

                                                   tttCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACt                                           <  2:3026744/100‑1 (MQ=255)

                                                     tctcAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTaa                                         >  1:3383022/1‑100 (MQ=255)

                                                     tctcAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTaa                                         >  2:2813018/1‑100 (MQ=255)

                                                      ctcAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAAc                                        >  2:3417325/1‑100 (MQ=255)

                                                      ctcAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAAc                                        >  1:454128/1‑100 (MQ=255)

                                                      atcAACGCCGTGCTATGGCAATGCAAAGAAGGGCATGGGCAACTGTCTGACGTGATTCTGGCTTTGCCTGCCACTTCGGCTACGGTATGGATGAACTAAc                                        <  2:3734118/99‑1 (MQ=255)

                                                       tcAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACg                                       >  1:2891683/1‑100 (MQ=255)

                                                            gcggggcaatagcaaggCGAAGGAGGGCACGGGCAAGGGAATGGCGGGATGCAGGCTTCGACGGAAATTTCGGCTACGGTATGGATGAACTAACGTTTta                                  <  2:286685/95‑1 (MQ=255)

                                                            gCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                                  <  2:2318951/100‑1 (MQ=255)

                                                            gCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                                  >  2:855717/1‑100 (MQ=255)

                                                              cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                                >  2:2145525/1‑100 (MQ=255)

                                                                tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAAc                              <  1:1699893/100‑1 (MQ=255)

                                                                    atggcaatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt                          <  2:70153/100‑1 (MQ=255)

                                                                    atggcaatgCAAAGAAGGGCATGGGCAACTGCCTGAAGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTACGGATCCACTAACGTTTTATAACGCCt                          >  2:1504652/1‑100 (MQ=255)

                                                                      ggcaatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTg                        >  1:3951894/1‑100 (MQ=255)

                                                                      ggcaatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTg                        >  1:1625422/1‑100 (MQ=255)

                                                                       gcaatgCAAAGAAGGGGATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGc                       <  2:3778470/100‑1 (MQ=255)

                                                                       gcaatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGc                       >  2:2869122/1‑100 (MQ=255)

                                                                       gcaatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGc                       >  1:37463/1‑100 (MQ=255)

                                                                        caatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCg                      <  2:721362/100‑1 (MQ=255)

                                                                        caatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCg                      <  1:2301489/100‑1 (MQ=255)

                                                                        caatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGAGTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCg                      >  2:3899513/1‑100 (MQ=255)

                                                                         aatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                     >  1:296706/1‑100 (MQ=255)

                                                                         aatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                     <  2:3089706/100‑1 (MQ=255)

                                                                         aatgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCCGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                     <  1:2000396/100‑1 (MQ=255)

                                                                          atgCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTg                    <  2:883003/100‑1 (MQ=255)

                                                                            gCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAc                  <  2:2117174/100‑1 (MQ=255)

                                                                            gCAAAGAAGGGCATGGGCAACTGCCTGACGGGATTATGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAc                  >  2:3904348/1‑100 (MQ=255)

                                                                             taaaGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACg                 <  1:3068365/99‑1 (MQ=255)

                                                                             cAAAGAAGGGCATGGGCAACTGCCTGGCGGGTTTCTGTCTTTGCCTGCCAGTTCGGCTACTGTATGGATTAACTAACGTTTTATAACTCCTTGCGTGACg                 >  2:3586201/1‑100 (MQ=255)

                                                                             cAAAGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACg                 >  2:3579797/1‑100 (MQ=255)

                                                                              aaaGAAGGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGc                >  1:557470/1‑100 (MQ=255)

                                                                               aagaagGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCTGCTACGGTATGGATGAACTAATGTTTTATAACGCCTTGCGTGACGcc               <  1:3649559/100‑1 (MQ=255)

                                                                                   agGGCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTgcg           >  1:2237813/1‑100 (MQ=255)

                                                                                    gggCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTgcgc          <  2:55164/100‑1 (MQ=255)

                                                                                    gggCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTgcgc          <  1:1657230/100‑1 (MQ=255)

                                                                                     ggCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGcc         <  2:349075/100‑1 (MQ=255)

                                                                                     ggCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGcc         <  2:2450906/100‑1 (MQ=255)

                                                                                     ggCATGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGcc         <  2:907889/100‑1 (MQ=255)

                                                                                       cATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcat       <  1:1829270/100‑1 (MQ=255)

                                                                                        aTGGGCAACTGCCTGACGGGGTTCTGGCTTTGCCTGCCATTTCGGCTACGGCATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatc      >  2:201607/1‑100 (MQ=255)

                                                                                        aTGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatc      >  1:2155006/1‑100 (MQ=255)

                                                                                        aTGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatc      >  2:2013944/1‑100 (MQ=255)

                                                                                         tGGGCAACTGCCTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatca     <  1:2661197/100‑1 (MQ=255)

GTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCC  >  NC_000913/424180‑424371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`