Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,566 A→C L48W (TTG→TGG)  tomB ← Hha toxicity attenuator; conjugation‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,5660AC97.6% 560.6 / ‑5.4 166L48W (TTG→TGG) tomBHha toxicity attenuator; conjugation‑related protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base C (70/92);  total (73/93)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACT  >  NC_000913/480469‑480665
                                                                                                 |                                                                                                   
     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                              >  2:3635689/1‑100 (MQ=255)
      ttCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAgg                                                                                             <  1:343228/100‑1 (MQ=255)
      tcttccgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAgg                                                                                             <  2:505142/95‑1 (MQ=255)
       tcgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGt                                                                                            <  2:3002367/100‑1 (MQ=255)
        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           <  2:2669603/100‑1 (MQ=255)
        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           >  1:1489937/1‑100 (MQ=255)
        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           >  1:3269796/1‑100 (MQ=255)
         ntcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTg                                                                                          >  1:3260714/2‑100 (MQ=255)
         gtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTg                                                                                          <  2:3343603/100‑1 (MQ=255)
          tcgAGCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGa                                                                                         <  1:3424543/100‑1 (MQ=255)
               cTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGcc                                                                                    >  2:2942370/1‑100 (MQ=255)
                tGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCg                                                                                   >  1:2319149/1‑100 (MQ=255)
                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:2502421/1‑100 (MQ=255)
                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:363024/1‑100 (MQ=255)
                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:3864124/1‑100 (MQ=255)
                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  <  2:1351084/100‑1 (MQ=255)
                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  2:1464063/1‑100 (MQ=255)
                  cTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAg                                                                                 <  1:3993416/100‑1 (MQ=255)
                   tCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAgg                                                                                <  1:21164/100‑1 (MQ=255)
                    cAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGt                                                                               >  2:2504645/1‑100 (MQ=255)
                     aaTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGtt                                                                              <  2:3912852/100‑1 (MQ=255)
                     aaTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGtt                                                                              >  1:2414815/1‑100 (MQ=255)
                      aTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTg                                                                             <  2:3876203/100‑1 (MQ=255)
                       tGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTgg                                                                            >  2:2521851/1‑100 (MQ=255)
                        gcgCTTATTGTCTTCATTATACTTAATTTTTTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATGCCACTGGAGGTTGATCGCCGAGGTTggg                                                                           <  1:1730033/98‑1 (MQ=255)
                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          <  1:2916875/100‑1 (MQ=255)
                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          <  1:2312527/100‑1 (MQ=255)
                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          >  2:122245/1‑100 (MQ=255)
                          gCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTc                                                                         <  2:1154140/100‑1 (MQ=255)
                            ttATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGt                                                                       >  2:1901630/1‑100 (MQ=255)
                             tATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGtt                                                                      <  1:2604492/100‑1 (MQ=255)
                              aTTGTCTTCATTATACTTGATTTTGTAATTAAGTGCGGAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGttt                                                                     <  2:334974/100‑1 (MQ=255)
                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                   >  2:2180372/1‑100 (MQ=255)
                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                   >  2:321809/1‑100 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  <  1:3349399/100‑1 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  >  1:3394424/1‑100 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  <  1:3274337/100‑1 (MQ=255)
                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  >  1:310420/1‑100 (MQ=255)
                                  tCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                 <  1:1354117/100‑1 (MQ=255)
                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                <  2:2801209/100‑1 (MQ=255)
                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                <  1:1555275/100‑1 (MQ=255)
                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                >  1:3691410/1‑100 (MQ=255)
                                     tCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGc                                                              <  2:1411052/100‑1 (MQ=255)
                                          ataCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGa                                                         <  1:824620/100‑1 (MQ=255)
                                           taCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAt                                                        <  1:1986428/100‑1 (MQ=255)
                                            aCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAtt                                                       <  2:3517296/100‑1 (MQ=255)
                                             cTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTg                                                      >  1:1957421/1‑100 (MQ=255)
                                                aaTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                   <  1:894230/100‑1 (MQ=255)
                                                 aTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCttt                                                  <  1:2598511/100‑1 (MQ=255)
                                                   tttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct                                                <  1:3498872/100‑1 (MQ=255)
                                                    ttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttctt                                               <  1:436599/100‑1 (MQ=255)
                                                     tGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttcttc                                              <  1:3037431/100‑1 (MQ=255)
                                                      gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCa                                             >  2:3091317/1‑100 (MQ=255)
                                                       tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCaa                                            >  2:1450505/1‑100 (MQ=255)
                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                          <  2:2711072/100‑1 (MQ=255)
                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                          >  1:2640877/1‑100 (MQ=255)
                                                             agggCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTg                                      >  2:3214506/4‑100 (MQ=255)
                                                              gTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                     >  1:2279055/1‑100 (MQ=255)
                                                               tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCTATGATTGCTTTCTTCAAGGTTTGCa                                    <  1:1170013/100‑1 (MQ=255)
                                                               tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa                                    >  2:690818/1‑100 (MQ=255)
                                                                gCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCaa                                   >  1:3936028/1‑100 (MQ=255)
                                                                 cGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg                                  <  2:794951/100‑1 (MQ=255)
                                                                 cGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg                                  >  1:2543786/1‑100 (MQ=255)
                                                                   aaGGTCGCAATATGCTCAATGAGTTCATTCCGCTGGAGGTTGTTCGCCGAGGTTGGGTCGTTTACCCAGCGATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:1471361/100‑1 (MQ=255)
                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:1327393/100‑1 (MQ=255)
                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:2026847/100‑1 (MQ=255)
                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  1:2209738/100‑1 (MQ=255)
                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg                              <  2:2283736/100‑1 (MQ=255)
                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCACGGCAGt                             >  1:487070/1‑100 (MQ=255)
                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGt                             >  2:2080341/1‑100 (MQ=255)
                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGt                             <  2:2463962/100‑1 (MQ=255)
                                                                        cGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa                           <  2:2105602/100‑1 (MQ=255)
                                                                         gCAATATGCTCAATCGGTTCATGCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                          <  2:861480/100‑1 (MQ=255)
                                                                         gCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                          >  1:1023443/1‑100 (MQ=255)
                                                                           aaTATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                        >  2:1660906/1‑100 (MQ=255)
                                                                               tGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACa                    >  1:857308/1‑100 (MQ=255)
                                                                               tGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGAGACa                    <  1:3138576/100‑1 (MQ=255)
                                                                                 cTCAATCAGTTCATTCCGTTGGAGGTTGATCGCCGAGGTGGGGTCGTTTAGCCAGCCATGATTTCTTTCTTCAAGGTTTTCAAGGCAGTCATGATACAgg                  >  2:2870763/1‑100 (MQ=255)
                                                                                 cTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg                  <  1:2924169/100‑1 (MQ=255)
                                                                                 cTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg                  <  2:1038478/100‑1 (MQ=255)
                                                                                  tCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAggg                 <  2:825081/100‑1 (MQ=255)
                                                                                   cAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGt                >  2:1553916/1‑100 (MQ=255)
                                                                                     aTCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt              <  2:1003492/100‑1 (MQ=255)
                                                                                     aTCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt              <  1:787462/100‑1 (MQ=255)
                                                                                      tCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTc             <  1:2467597/100‑1 (MQ=255)
                                                                                       ctgTTCTTTAAACTGGAGGTTGATCGTCGTGGTTGGGTAGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            <  1:763894/98‑1 (MQ=255)
                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            >  2:1134241/1‑100 (MQ=255)
                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            <  1:2137242/100‑1 (MQ=255)
                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            >  2:938621/1‑100 (MQ=255)
                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1589228/100‑1 (MQ=255)
                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1688861/100‑1 (MQ=255)
                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1842937/100‑1 (MQ=255)
                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  2:98344/100‑1 (MQ=255)
                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTAGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCACGGCAGTCATGATACAGGGTTTcac           >  2:382435/1‑100 (MQ=255)
                                                                                         gTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca          >  1:2969045/1‑100 (MQ=255)
                                                                                           tcattccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga        <  1:407453/100‑1 (MQ=255)
                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     >  1:3728497/1‑100 (MQ=255)
                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     >  2:1848520/1‑100 (MQ=255)
                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     <  2:2354045/100‑1 (MQ=255)
                                                                                                 aaCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACt  <  2:304997/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACT  >  NC_000913/480469‑480665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑