Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 25,474 T→G S90A (TCG→GCG)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091325,4740TG98.7% 543.8 / ‑4.4 157S90A (TCG→GCG) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (79/76);  total (80/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCG  >  NC_000913/25380‑25573
                                                                                              |                                                                                                   
tAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGGTCCTGGCAGTGATGATGTATCGCGCGAAg                                                                                                <  2:1286150/100‑1 (MQ=255)
tAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGaan                                                                                                <  1:1144791/100‑2 (MQ=255)
tAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAg                                                                                                >  1:2855072/1‑100 (MQ=255)
 aGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGTCGGTATTGTGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTTTCGCGCGAAgg                                                                                               >  2:580496/1‑100 (MQ=255)
 aGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTCTTAGCGTGATCTTGGCAGTGATGATGTATCGCGCGAAgg                                                                                               >  1:1765872/1‑100 (MQ=255)
  gTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGc                                                                                              <  2:2954354/100‑1 (MQ=255)
  gTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGc                                                                                              >  1:1771248/1‑100 (MQ=255)
    ttCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCa                                                                                            >  1:3696817/1‑100 (MQ=255)
     tCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCAc                                                                                           <  2:3793085/100‑1 (MQ=255)
     tCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCAc                                                                                           >  1:536876/1‑100 (MQ=255)
     tCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCAc                                                                                           >  1:180882/1‑100 (MQ=255)
      ccTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACg                                                                                          <  2:1360407/100‑1 (MQ=255)
       cTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGc                                                                                         <  2:2161414/100‑1 (MQ=255)
       cTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGc                                                                                         <  2:3780065/100‑1 (MQ=255)
       cTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGc                                                                                         <  2:3659332/100‑1 (MQ=255)
         tGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAg                                                                                       <  1:3276820/100‑1 (MQ=255)
          gCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGa                                                                                      >  1:2186457/1‑100 (MQ=255)
           ccGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGaa                                                                                     >  1:3986214/1‑100 (MQ=255)
           cagATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATTCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGaa                                                                                     <  2:3281863/98‑1 (MQ=255)
             gacaGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGc                                                                                   <  2:2647968/97‑1 (MQ=255)
             gATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGc                                                                                   >  2:1752682/1‑100 (MQ=255)
               tAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTa                                                                                 <  2:497002/100‑1 (MQ=255)
               tAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTa                                                                                 <  1:3837489/100‑1 (MQ=255)
                aGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTaa                                                                                <  2:496288/100‑1 (MQ=255)
                    gcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACaa                                                                            <  1:605003/100‑1 (MQ=255)
                      ggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAata                                                                          <  1:3777610/100‑1 (MQ=255)
                      ggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAata                                                                          >  2:163111/1‑100 (MQ=255)
                       gcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAatat                                                                         <  1:424328/100‑1 (MQ=255)
                        cTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATc                                                                        <  1:1537040/100‑1 (MQ=255)
                         tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCg                                                                       <  2:316855/100‑1 (MQ=255)
                         tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCg                                                                       <  1:1210585/100‑1 (MQ=255)
                          ggCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGc                                                                      >  2:2527325/1‑100 (MQ=255)
                           gCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCt                                                                     >  2:2272766/1‑100 (MQ=255)
                           gCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCt                                                                     <  2:526268/100‑1 (MQ=255)
                            cAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCtt                                                                    <  1:473552/100‑1 (MQ=255)
                            cAGCGTGGGTTCTGCGCCGGTATTGCGATTGGTATTGGCGTGATCCCGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCtt                                                                    <  2:2015077/100‑1 (MQ=255)
                               cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAcg                                                                 >  1:3530344/1‑100 (MQ=255)
                                gTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTAcgc                                                                >  1:239565/1‑100 (MQ=255)
                                  tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACgcgc                                                              >  1:2040242/1‑100 (MQ=255)
                                   ggTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCt                                                             >  2:2165947/1‑100 (MQ=255)
                                   ggTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCt                                                             >  1:3175043/1‑100 (MQ=255)
                                   ggTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCt                                                             >  2:1847492/1‑100 (MQ=255)
                                    gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTg                                                            <  1:1881040/100‑1 (MQ=255)
                                     ttCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTgc                                                           >  2:1925643/1‑99 (MQ=255)
                                      tCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGCATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGat                                                          >  1:3955029/1‑100 (MQ=255)
                                       cTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGatt                                                         >  2:1839988/1‑100 (MQ=255)
                                        tttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGatta                                                        >  1:2708050/1‑100 (MQ=255)
                                          tGCCGGAATTGCGATTGGCATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGcttatt                                                      >  1:3399292/1‑100 (MQ=255)
                                            ccGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTgg                                                    >  2:1266092/1‑100 (MQ=255)
                                            ccGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTgg                                                    >  1:164979/1‑100 (MQ=255)
                                             cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggc                                                   <  1:2333685/100‑1 (MQ=255)
                                             cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggc                                                   <  1:921230/100‑1 (MQ=255)
                                             cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggc                                                   <  1:3923758/100‑1 (MQ=255)
                                             cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggc                                                   <  2:3371988/100‑1 (MQ=255)
                                              ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcg                                                  <  1:4029577/100‑1 (MQ=255)
                                              ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcg                                                  <  1:3092908/100‑1 (MQ=255)
                                               gTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcgg                                                 <  1:1682457/100‑1 (MQ=255)
                                                tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTggcggc                                                <  1:1899215/100‑1 (MQ=255)
                                                 aTTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGgcggcg                                               >  2:3122746/1‑100 (MQ=255)
                                                   tGCGATTGGTGTTAGCGTGATCCTTGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcc                                             >  2:3677556/1‑99 (MQ=255)
                                                   tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcg                                             >  1:809029/1‑100 (MQ=255)
                                                   tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcg                                             >  1:1577407/1‑100 (MQ=255)
                                                    gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcgc                                            >  2:710992/1‑100 (MQ=255)
                                                    gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcgc                                            >  2:3885327/1‑100 (MQ=255)
                                                    gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGgcgcgc                                            >  1:1599277/1‑100 (MQ=255)
                                                     cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCt                                           >  2:1102343/1‑100 (MQ=255)
                                                      gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTg                                          >  2:2718251/1‑100 (MQ=255)
                                                          ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCa                                      >  1:939751/1‑100 (MQ=255)
                                                           ntATTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGACGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCaa                                     >  1:3237715/2‑100 (MQ=255)
                                                           gTACTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCaa                                     >  1:3135631/1‑100 (MQ=255)
                                                             aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                   <  2:2595640/100‑1 (MQ=255)
                                                             aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                   <  1:1316716/100‑1 (MQ=255)
                                                             aTTAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAAcc                                   <  2:3102449/100‑1 (MQ=255)
                                                               tAGCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTg                                 >  1:465789/1‑100 (MQ=255)
                                                                 gCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGtt                               >  1:2337750/1‑100 (MQ=255)
                                                                 gCGTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGtt                               >  1:1667997/1‑100 (MQ=255)
                                                                   gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                             >  2:1659704/1‑100 (MQ=255)
                                                                   gTGATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCg                             >  2:463851/1‑100 (MQ=255)
                                                                     gATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGAc                           <  2:758288/100‑1 (MQ=255)
                                                                     gATCCTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGAc                           >  2:3036290/1‑100 (MQ=255)
                                                                     caTCCTGTCAGTTATGATCTATCGCGCGCAGGCCACGCATAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGAc                           <  2:3336088/99‑1 (MQ=255)
                                                                         cTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGcc                       >  1:1101705/1‑100 (MQ=255)
                                                                         cTGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGcc                       >  1:1102027/1‑100 (MQ=255)
                                                                          tGGCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCt                      >  2:419321/1‑100 (MQ=255)
                                                                            gCAGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCtgt                    >  1:418626/1‑100 (MQ=255)
                                                                              aGTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTgg                  <  1:1544563/100‑1 (MQ=255)
                                                                               gTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTTGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGc                 >  2:626420/1‑100 (MQ=255)
                                                                               gTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGc                 <  1:2585350/100‑1 (MQ=255)
                                                                               gTGATGATGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGAGCAACCTGTTCGACCGCCTGTGGc                 <  2:725301/100‑1 (MQ=255)
                                                                                 gatgatGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCAc               <  1:333268/100‑1 (MQ=255)
                                                                                 gatgatGTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCAc               >  2:2087998/1‑100 (MQ=255)
                                                                                   tgatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACgg             <  2:1346545/100‑1 (MQ=255)
                                                                                   tgatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACgg             >  1:1398068/1‑100 (MQ=255)
                                                                                    gatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTTCGCGCTGATTTTTGTCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGc            >  1:3788506/1‑100 (MQ=255)
                                                                                    gatgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGc            <  2:2276462/100‑1 (MQ=255)
                                                                                     atgTATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCt           >  1:2753571/1‑100 (MQ=255)
                                                                                        tATCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCg        <  1:1347026/100‑1 (MQ=255)
                                                                                         aTCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt       >  1:2508929/1‑100 (MQ=255)
                                                                                         aTCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt       >  1:3157288/1‑100 (MQ=255)
                                                                                         aTCGCGCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATCATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGt       >  2:2594841/1‑100 (MQ=255)
                                                                                              |                                                                                                   
TAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCG  >  NC_000913/25380‑25573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: