Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 546,173 T→G E64A (GAA→GCA)  allD ← ureidoglycolate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913546,1730TG97.0% 545.3 / 3.4 168E64A (GAA→GCA) allDureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (82/81);  total (84/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 169 total aligned reads displayed.

ATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATA  >  NC_000913/546076‑546269
                                                                                                 |                                                                                                
 tGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACCCCGGTgcgc                                                                                               >  2:3456472/1‑100 (MQ=255)
    tCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCgga                                                                                            >  2:418947/1‑99 (MQ=255)
     ccATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGtt                                                                                           <  1:2931930/100‑1 (MQ=255)
     acATACCCAATGTCGCGGCGACCTGTCCGGCGGCAGTGTCGGCATGTAAGGTTGCCGAGCACGGCCCGGTTTCCTCGAGACGAATCTCCGGTGAGCGGtt                                                                                           <  2:1495772/99‑1 (MQ=255)
      cATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTg                                                                                          <  1:880250/100‑1 (MQ=255)
        tACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGt                                                                                        >  1:2534869/1‑100 (MQ=255)
          cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGTCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  2:512698/1‑100 (MQ=255)
          cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  1:3434064/1‑100 (MQ=255)
          cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  1:3203388/1‑100 (MQ=255)
          cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgc                                                                                      >  2:1863559/1‑100 (MQ=255)
          cccATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGGTGGTgc                                                                                      >  2:847379/1‑100 (MQ=255)
            cctttgCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgccg                                                                                    <  2:1700883/98‑1 (MQ=255)
             aTTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTgccgc                                                                                   >  2:3154681/1‑100 (MQ=255)
               tttCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCt                                                                                 <  1:1637988/100‑1 (MQ=255)
               tttCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCt                                                                                 >  2:1193290/1‑100 (MQ=255)
                 tCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCttt                                                                               <  1:1458027/100‑1 (MQ=255)
                  cgccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCtttt                                                                              <  2:874417/100‑1 (MQ=255)
                  cgccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGGTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCGtttt                                                                              >  1:3656096/1‑100 (MQ=255)
                   gccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTg                                                                             <  1:1749337/100‑1 (MQ=255)
                   gccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTg                                                                             >  1:3487183/1‑100 (MQ=255)
                   gccgcGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTg                                                                             <  1:164718/100‑1 (MQ=255)
                     cgcgACCTGTCCGGCGGCATTGTCGGCATATAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGaa                                                                           <  2:192677/100‑1 (MQ=255)
                      gcgACCGGTCCGGCGGCATTGTCGGCAGGTAAAATTGCCGGGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGGGGTTGGTGCCGCCTTTTGaaa                                                                          <  2:482957/100‑1 (MQ=255)
                       cgACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAAt                                                                         <  2:3088503/100‑1 (MQ=255)
                        gACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATg                                                                        <  2:1196807/100‑1 (MQ=255)
                          ccTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCg                                                                      >  2:3964847/1‑100 (MQ=255)
                           cTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTGAAATGCGtt                                                                    <  2:1489584/100‑1 (MQ=255)
                             gTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTc                                                                   >  2:110781/1‑100 (MQ=255)
                               ccGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcg                                                                 <  1:3138186/100‑1 (MQ=255)
                               ccGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcg                                                                 >  1:1088875/1‑100 (MQ=255)
                                cggcggCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgc                                                                >  1:2333199/1‑100 (MQ=255)
                                cggcggCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgc                                                                >  1:214266/1‑100 (MQ=255)
                                 ggcggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCcgcg                                                               <  1:1103339/100‑1 (MQ=255)
                                  gcggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgt                                                              <  2:370962/100‑1 (MQ=255)
                                    ggcATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCgtag                                                            <  1:3466823/100‑1 (MQ=255)
                                       aTTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTAt                                                         >  2:1097500/1‑100 (MQ=255)
                                         tGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTc                                                       >  1:3963831/1‑100 (MQ=255)
                                          gTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTcc                                                      >  2:2279636/1‑100 (MQ=255)
                                           tCGGCATGTAAAATTGCCGAGCATGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCa                                                     <  2:464618/100‑1 (MQ=255)
                                           tCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCa                                                     <  2:2360283/100‑1 (MQ=255)
                                           gatctaTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCa                                                     <  1:2261299/95‑1 (MQ=255)
                                            cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGCAATGCGTTCCGCGTAGTATTCCAc                                                    <  1:2662690/100‑1 (MQ=255)
                                            cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAc                                                    <  2:2297576/100‑1 (MQ=255)
                                            cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAc                                                    <  2:2516839/100‑1 (MQ=255)
                                            cGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTGTTGAAATGCGTTCCGCGTAGTATTCCAc                                                    >  2:1867304/1‑100 (MQ=255)
                                            cGGCATGTAAAATTGCCGAGCACGGCCCGGTGTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTGGTGAAATTCGTTCCGCGTAGTGTTCCAc                                                    >  2:582354/1‑100 (MQ=255)
                                             ggCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAcg                                                   >  1:776341/1‑100 (MQ=255)
                                             ggCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAcg                                                   >  1:1177546/1‑100 (MQ=255)
                                              gCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCAcgc                                                  <  2:3057059/100‑1 (MQ=255)
                                                aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCaagatc                                                >  2:2226650/1‑95 (MQ=255)
                                                aTGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACgcgc                                                >  2:2599425/1‑100 (MQ=255)
                                                 tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               >  1:474563/1‑100 (MQ=255)
                                                 tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               >  1:1847548/1‑100 (MQ=255)
                                                 tGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCa                                               <  2:3851872/100‑1 (MQ=255)
                                                 tGTAAAATTACCGAGCACGGCCCGGTTTCCTCGAGACGAAACTCCTGTGTGCGGGCGGTGCCGCCTTTTGAAATGCGTTCCGCGGAGTTTTGCTCGGGCa                                               >  2:3208951/1‑100 (MQ=255)
                                                  gTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCAc                                              >  2:3228709/1‑100 (MQ=255)
                                                   tAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGCTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCAcc                                             >  1:431509/1‑100 (MQ=255)
                                                          gCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCAt                                      >  1:2577726/1‑100 (MQ=255)
                                                          gCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCAt                                      >  2:1133613/1‑100 (MQ=255)
                                                           ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCTGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     >  2:3188676/1‑100 (MQ=255)
                                                           ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     <  2:2728441/100‑1 (MQ=255)
                                                           ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     >  1:1054190/1‑100 (MQ=255)
                                                           ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATg                                     <  2:3407521/100‑1 (MQ=255)
                                                           ccGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGAGTAGTATTCCACGCGCACCGCGCCATg                                     <  2:332524/100‑1 (MQ=255)
                                                            cGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATga                                    <  1:2248613/100‑1 (MQ=255)
                                                            cGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGGGGGTGCCGCCTTTTGACATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATga                                    >  1:180839/1‑100 (MQ=255)
                                                             gAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATgag                                   >  2:1792074/1‑100 (MQ=255)
                                                             gAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATgag                                   <  1:1543780/100‑1 (MQ=255)
                                                               gCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGagag                                 <  2:833819/100‑1 (MQ=255)
                                                                cACGGCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGt                                >  1:3014399/1‑100 (MQ=255)
                                                                 aCGGCCCGGTTTCCTCAAGACGAACCTCCGGTGCGGGGTTGGTGGCGCCTTTTGAAATGCTTTCCGCGTAGTATTCCACGCGCACCGCGTCATGGGAGTg                               <  2:1447707/100‑1 (MQ=255)
                                                                   ggCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGa                             >  1:622004/1‑100 (MQ=255)
                                                                   ggCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGa                             >  2:2840612/1‑100 (MQ=255)
                                                                    gCCCGGTTTCCTCAAGACGGAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTCGTATTCCACGCGCACCGCCCCATGACAGCGGAt                            >  2:3656176/1‑100 (MQ=255)
                                                                    gCCCGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGAt                            <  1:3336664/100‑1 (MQ=255)
                                                                     cccGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATc                           >  1:3473530/1‑100 (MQ=255)
                                                                      ccGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATcc                          <  2:3783339/100‑1 (MQ=255)
                                                                      ccGGTTTCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATcc                          <  1:847278/100‑1 (MQ=255)
                                                                          tttCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCctct                      <  1:1370933/100‑1 (MQ=255)
                                                                           ttCCTCAAGACGAAACTCCGGTGCGCTGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTg                     <  2:3594230/100‑1 (MQ=255)
                                                                           ttCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTg                     <  2:1114174/100‑1 (MQ=255)
                                                                            tCCTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTgg                    <  2:2789216/100‑1 (MQ=255)
                                                                             ccTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGc                   >  1:3039706/1‑100 (MQ=255)
                                                                              cTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCGCCGCGCCATGAGAGTGGATCCCTCTGGCa                  <  1:4013998/100‑1 (MQ=255)
                                                                              cTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCa                  >  1:884818/1‑100 (MQ=255)
                                                                              cTCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCa                  <  2:3161163/100‑1 (MQ=255)
                                                                               tCAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCAt                 <  1:2717079/100‑1 (MQ=255)
                                                                                cAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATc                <  2:2229967/100‑1 (MQ=255)
                                                                                cAAGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCCGGCATc                >  1:2075724/1‑100 (MQ=255)
                                                                                 aaGACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCg               <  1:112559/100‑1 (MQ=255)
                                                                                   gACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGc             >  1:3545436/1‑100 (MQ=255)
                                                                                   gACGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGc             <  1:561313/100‑1 (MQ=255)
                                                                                     cGAAACTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGt           >  1:2827913/1‑100 (MQ=255)
                                                                                       aaaCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTaa         >  2:721216/1‑100 (MQ=255)
                                                                                       aaaCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTaa         >  2:797357/1‑100 (MQ=255)
                                                                                        aaCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTaaa        >  2:997581/1‑100 (MQ=255)
                                                                                         aCTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTCCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAc       <  1:3898677/100‑1 (MQ=255)
                                                                                          cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      <  2:760058/100‑1 (MQ=255)
                                                                                          cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      >  2:2248124/1‑100 (MQ=255)
                                                                                          cTCCGGTGCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAAcc      <  1:1081256/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATGTTCCATACCCATTTTCGCCGCGACCTGTCCGGCGGCATTGTCGGCATGTAAAATTGCCGAGCACGGCCCGGTTTCCTCAAGACGAAACTCCGGTTCGCGGTTGGTGCCGCCTTTTGAAATGCGTTCCGCGTAGTATTCCACGCGCACCGCGCCATGAGAGTGGATCCCTCTGGCATCGGCGTAAACCAATA  >  NC_000913/546076‑546269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: