Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 623,458 T→G L387V (TTG→GTG)  entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913623,4580TG97.8% 481.4 / ‑3.1 139L387V (TTG→GTG) entSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (60/74);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGC  >  NC_000913/623363‑623553
                                                                                               |                                                                                               
aGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTgtgtt                                                                                             <  1:3051642/100‑1 (MQ=255)
 gAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTgtgttg                                                                                            >  1:2370032/1‑100 (MQ=255)
  aaCGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGa                                                                                           >  2:764553/1‑100 (MQ=255)
   aCGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGAt                                                                                          >  1:3836713/1‑100 (MQ=255)
      tGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTAt                                                                                       >  1:681439/1‑100 (MQ=255)
      tGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGGGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTAt                                                                                       >  1:1673780/1‑100 (MQ=255)
        aCGGGAGATGACATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTCGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCg                                                                                     <  2:2195079/100‑1 (MQ=255)
         cGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCgg                                                                                    >  1:896036/1‑100 (MQ=255)
           ggCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCg                                                                                  <  1:2916346/100‑1 (MQ=255)
           ggCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCg                                                                                  <  1:3840161/100‑1 (MQ=255)
           ggCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCg                                                                                  >  1:1820715/1‑100 (MQ=255)
           ggCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCg                                                                                  >  1:3637187/1‑100 (MQ=255)
             cGATGCGATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCgtg                                                                                <  2:3796825/100‑1 (MQ=255)
                tGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgttg                                                                             >  2:699333/1‑100 (MQ=255)
                 gCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgttgt                                                                            <  2:3857239/100‑1 (MQ=255)
                 gCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgttgt                                                                            >  1:4048230/1‑100 (MQ=255)
                 accATCGGCCCGTCGCTGTTGACTCTTTTGCACGCGATTATGACACCGGTTGGTTACCAAAGCGCGACCGGTTTTCATCTGTTGATTATCGGCGtgttgt                                                                            <  2:479250/99‑1 (MQ=255)
                   cATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTa                                                                          >  2:1438443/1‑100 (MQ=255)
                   cATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTa                                                                          >  2:1155264/1‑100 (MQ=255)
                   cATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTa                                                                          <  1:340745/100‑1 (MQ=255)
                    aTAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAt                                                                         <  1:3441867/100‑1 (MQ=255)
                     tAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAtt                                                                        <  1:3511874/100‑1 (MQ=255)
                      aGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtg                                                                       <  1:2712860/100‑1 (MQ=255)
                      aGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtg                                                                       <  2:742499/100‑1 (MQ=255)
                       ggcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgc                                                                      >  1:2755350/1‑100 (MQ=255)
                       ggcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgc                                                                      >  1:379079/1‑100 (MQ=255)
                       ggcgcggcacTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgc                                                                      <  2:1039809/100‑1 (MQ=255)
                         cgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctg                                                                    >  2:2702078/1‑100 (MQ=255)
                          gcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctgg                                                                   <  2:3919964/100‑1 (MQ=255)
                          gcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctgg                                                                   <  2:521234/100‑1 (MQ=255)
                          gcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctgg                                                                   <  1:903341/100‑1 (MQ=255)
                           cggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggt                                                                  >  2:554741/1‑100 (MQ=255)
                           cggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggt                                                                  >  1:2203434/1‑100 (MQ=255)
                           cggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggt                                                                  <  1:1998355/100‑1 (MQ=255)
                             gcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggtgc                                                                >  1:1612130/1‑100 (MQ=255)
                              cgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggtgct                                                               <  1:280547/100‑1 (MQ=255)
                              cgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggtgct                                                               >  2:709964/1‑100 (MQ=255)
                                 tgctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTgctggtgctggt                                                            <  1:2948471/100‑1 (MQ=255)
                                 tgctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTgctggtgctggt                                                            >  1:1586669/1‑100 (MQ=255)
                                  gctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGctggtgctggtg                                                           <  2:1234954/100‑1 (MQ=255)
                                  gctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGctggtgctggtg                                                           <  2:431001/100‑1 (MQ=255)
                                  gctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGctggtgctggtg                                                           <  2:95119/100‑1 (MQ=255)
                                  gctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGctggtgctggtg                                                           <  1:2106343/100‑1 (MQ=255)
                                   ctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCtggtgg                                                          <  1:608882/100‑1 (MQ=255)
                                   ctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCtggtgg                                                          <  1:3120934/100‑1 (MQ=255)
                                     gggTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGGGCGGTTTTGGTGTGTTGCTTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAg                                                        <  1:1992964/100‑1 (MQ=255)
                                      ggtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGt                                                       >  2:543739/1‑100 (MQ=255)
                                      ggtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGt                                                       <  1:2149550/100‑1 (MQ=255)
                                      ggtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGt                                                       <  2:3131263/100‑1 (MQ=255)
                                       gtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGtt                                                      >  2:914339/1‑100 (MQ=255)
                                        tggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTg                                                     <  1:1123401/100‑1 (MQ=255)
                                         ggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGc                                                    >  1:2395927/1‑100 (MQ=255)
                                          gtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCg                                                   <  1:580102/100‑1 (MQ=255)
                                          gtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCg                                                   <  2:2177638/100‑1 (MQ=255)
                                                 cgcgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACAtttt                                            >  2:2683248/1‑100 (MQ=255)
                                                 cgcgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACAtttt                                            >  2:500509/1‑100 (MQ=255)
                                                     atgatgACACCGGTTGCTTCCGCAAGCGCGCGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGcc                                        <  1:1317844/100‑1 (MQ=255)
                                                     atgatgACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGcc                                        <  1:2630753/100‑1 (MQ=255)
                                                       gatgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTGGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAg                                      <  1:3844113/100‑1 (MQ=255)
                                                       catgaCACCGGTTGCTTCCGCAAGCGCGACCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAg                                      <  2:1620390/99‑1 (MQ=255)
                                                         tgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAc                                    >  1:2758793/1‑100 (MQ=255)
                                                         tgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAc                                    >  1:1237641/1‑100 (MQ=255)
                                                         tgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTGATTGCTGGTGCTGGGGGAGTTGCGACATTTTCGCCAGAc                                    >  2:3207975/1‑100 (MQ=255)
                                                         tgaCACCGGTTGCTGCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAc                                    <  2:3061195/100‑1 (MQ=255)
                                                           acacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgc                                  >  1:1176028/1‑100 (MQ=255)
                                                            cacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgcc                                 >  1:1222039/1‑100 (MQ=255)
                                                            cacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgcc                                 >  1:3100082/1‑100 (MQ=255)
                                                             tccGGCTGCTTCCACAAGCGCGAGCGGTTTTGGTGTGTTGATTATTGGCGTGTTGTTATTGCTGGTGCTGGTGCAGTTGCGACATATTCGCCAGAcgccg                                <  2:3733870/99‑1 (MQ=255)
                                                             acCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccg                                <  2:1512887/100‑1 (MQ=255)
                                                                ggTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccgccg                             >  1:4021088/1‑100 (MQ=255)
                                                                    gCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAgg                         >  2:3223474/1‑100 (MQ=255)
                                                                     cTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGt                        >  2:1049459/1‑100 (MQ=255)
                                                                        ccGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGAc                     >  2:1344927/1‑100 (MQ=255)
                                                                         cGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACa                    >  2:3861209/1‑100 (MQ=255)
                                                                         cGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACa                    >  2:2838878/1‑100 (MQ=255)
                                                                          gCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAg                   <  1:12684/100‑1 (MQ=255)
                                                                              gcgcGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTc               <  2:3461053/100‑1 (MQ=255)
                                                                              gcgcGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTc               >  1:3502359/1‑100 (MQ=255)
                                                                              gcgcGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTc               <  2:2354654/100‑1 (MQ=255)
                                                                               cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc              <  1:1472908/100‑1 (MQ=255)
                                                                               cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc              <  2:190643/100‑1 (MQ=255)
                                                                               cgcgAGCGGTTTTGGTCTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc              <  2:3347700/100‑1 (MQ=255)
                                                                                 cgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACCGCGTCCGa            >  2:1849114/1‑100 (MQ=255)
                                                                                 cgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGa            >  1:452077/1‑100 (MQ=255)
                                                                                  gAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGAc           <  2:2069784/100‑1 (MQ=255)
                                                                                  gAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGAc           <  2:1573386/100‑1 (MQ=255)
                                                                                  gAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGAc           >  1:3730456/1‑100 (MQ=255)
                                                                                  gAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGAc           >  1:1828426/1‑100 (MQ=255)
                                                                                    gCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAg         <  1:3279992/100‑1 (MQ=255)
                                                                                    gCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAg         >  2:2324290/1‑100 (MQ=255)
                                                                                    gCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAg         <  2:2792552/100‑1 (MQ=255)
                                                                                     cGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGt        >  2:1801832/1‑100 (MQ=255)
                                                                                      ggTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGtt       <  2:506540/100‑1 (MQ=255)
                                                                                      ggTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGtt       >  1:2813657/1‑100 (MQ=255)
                                                                                       gTTTTGGTTTGTTGAGTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGATTTGCGACATTTTCGCAAGACGGCGCCGCAGGTGACAGCGTCCGACAGTTa      <  2:2578650/100‑1 (MQ=255)
                                                                                       gTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa      <  2:3533057/100‑1 (MQ=255)
                                                                                         tttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAAt    <  1:3341283/100‑1 (MQ=255)
                                                                                          ttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATg   <  2:2116894/100‑1 (MQ=255)
                                                                                           tGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGc  <  1:3282785/100‑1 (MQ=255)
                                                                                           tGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGc  <  1:264032/100‑1 (MQ=255)
                                                                                               |                                                                                               
AGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGC  >  NC_000913/623363‑623553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: