Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 43,187 T→G intergenic (+14/‑1) fixA → / → fixB anaerobic carnitine reduction putative electron transfer flavoprotein subunit/putative electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091343,1870TG99.5% 697.2 / NA 198intergenic (+14/‑1)fixA/fixBanaerobic carnitine reduction putative electron transfer flavoprotein subunit/putative electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (78/118);  total (79/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 198 total aligned reads displayed.

CGCGAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAAC  >  NC_000913/43090‑43283
                                                                                                 |                                                                                                
cgcgAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGAt                                                                                                <  1:975616/100‑1 (MQ=255)
cgcgAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGAt                                                                                                <  1:879941/100‑1 (MQ=255)
cgcgAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCGGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGAt                                                                                                >  1:3837876/1‑100 (MQ=255)
 gcgAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATg                                                                                               >  2:533678/1‑100 (MQ=255)
 gcgAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATg                                                                                               <  1:2122221/100‑1 (MQ=255)
   gAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGaa                                                                                             <  1:4061400/100‑1 (MQ=255)
    aaCGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAac                                                                                            <  1:933150/100‑1 (MQ=255)
       gTCAGCGCATCGTGATTGAAGGCGACGGCTAAGAAAAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGGTGAACACg                                                                                         <  2:3827549/100‑1 (MQ=255)
       gTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACg                                                                                         >  1:1125633/1‑100 (MQ=255)
       gTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACg                                                                                         >  1:3365189/1‑100 (MQ=255)
          aGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGttt                                                                                      >  2:2701/1‑100 (MQ=255)
             gcATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTtct                                                                                   <  1:733598/100‑1 (MQ=255)
              cATCGTGATTGAAGGCGACGGTGAATAACAGATCGCAGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTtctc                                                                                  <  2:3784835/100‑1 (MQ=255)
              cATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTtctc                                                                                  >  2:3069388/1‑100 (MQ=255)
              cATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTtctc                                                                                  >  2:2157248/1‑100 (MQ=255)
               aTCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCa                                                                                 >  2:856408/1‑100 (MQ=255)
               aTCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCa                                                                                 >  2:3237174/1‑100 (MQ=255)
               aTCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCa                                                                                 <  1:2403306/100‑1 (MQ=255)
                tCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCaa                                                                                >  1:2355087/1‑100 (MQ=255)
                tCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCaa                                                                                >  1:2451930/1‑100 (MQ=255)
                  gTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGt                                                                              >  1:506460/1‑100 (MQ=255)
                   tGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTc                                                                             >  2:2186795/1‑100 (MQ=255)
                    gATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCt                                                                            >  1:2547234/1‑100 (MQ=255)
                      ttGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGTTGCGATGAACACGTCTTCTCAAGTCTgg                                                                          <  1:3587145/100‑1 (MQ=255)
                      ttGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTgg                                                                          >  1:1756290/1‑100 (MQ=255)
                       tGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTggg                                                                         <  1:2482360/100‑1 (MQ=255)
                        gAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGt                                                                        >  1:2841548/1‑100 (MQ=255)
                          aGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTAt                                                                      <  1:2940718/100‑1 (MQ=255)
                          aGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTAt                                                                      >  1:339004/1‑100 (MQ=255)
                          aGGCGACGGCGAAGAACAGATCGACGAATTTGATGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTAt                                                                      <  1:1620223/100‑1 (MQ=255)
                              gACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAg                                                                  <  1:3494771/100‑1 (MQ=255)
                               aCGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGc                                                                 <  2:2471580/100‑1 (MQ=255)
                                cGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCg                                                                <  1:2564096/100‑1 (MQ=255)
                                cGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCg                                                                >  2:2917592/1‑100 (MQ=255)
                                cGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCg                                                                <  1:2759729/100‑1 (MQ=255)
                                cGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGGCTGGGTATTCAGCg                                                                <  2:3901494/100‑1 (MQ=255)
                                cGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTCTTCTCAAGTCTGGGTATTCAGCg                                                                >  2:792604/1‑100 (MQ=255)
                                  gCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGAt                                                              >  2:3175085/1‑100 (MQ=255)
                                  gCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGAt                                                              <  1:3928527/100‑1 (MQ=255)
                                  gCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGAt                                                              <  1:597365/100‑1 (MQ=255)
                                   cGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATa                                                             >  2:2451246/1‑100 (MQ=255)
                                     aagaaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATAcc                                                           <  2:2641466/100‑1 (MQ=255)
                                        aaCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCt                                                        >  1:2782424/1‑100 (MQ=255)
                                         aCAGATCGCCGCATTTGCTTAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCtt                                                       >  2:2537563/1‑100 (MQ=255)
                                         aCAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCtt                                                       >  2:778715/1‑100 (MQ=255)
                                          cAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTtc                                                      >  2:3291793/1‑100 (MQ=255)
                                          cAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTtc                                                      >  2:1974937/1‑100 (MQ=255)
                                            gATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTtctc                                                    >  1:2825629/1‑100 (MQ=255)
                                            gATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTtctc                                                    >  1:2982583/1‑100 (MQ=255)
                                            gATAGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTCATTACAGGGGATGCGATGAACACGTTTTCTCCAGTCTGGGTATTCAGCGATACCCCTtctc                                                    >  2:3666633/1‑100 (MQ=255)
                                             ntCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCg                                                   >  1:3255311/2‑100 (MQ=255)
                                               cgccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTc                                                 <  2:2946282/100‑1 (MQ=255)
                                               cgccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTc                                                 >  2:300746/1‑100 (MQ=255)
                                               cgccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTc                                                 >  2:1792893/1‑100 (MQ=255)
                                               cgccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTc                                                 <  2:1412891/100‑1 (MQ=255)
                                                 ccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTCCAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTg                                               <  2:1465303/100‑1 (MQ=255)
                                                 ccgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTg                                               <  1:1548210/100‑1 (MQ=255)
                                                  cgcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGc                                              >  1:1976998/1‑100 (MQ=255)
                                                   gcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGcc                                             <  1:3224830/100‑1 (MQ=255)
                                                   gcATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGcc                                             <  1:454148/100‑1 (MQ=255)
                                                    cATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCg                                            <  1:1143664/100‑1 (MQ=255)
                                                    cATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCg                                            <  1:3383547/100‑1 (MQ=255)
                                                    cATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCg                                            <  1:1421488/100‑1 (MQ=255)
                                                     aTTTACTCAAAATCTTCGCAAAGTCATTTAATTACAGGGCATGCTATGAACACGTTTTCTCAAGTCTAGCTATTCAGCGATACCCCTTCTCGTCTGCCgg                                           <  2:2885777/100‑1 (MQ=255)
                                                         gttGAAAATCATCGCAAAGTCATTTAATTCAAGGGGATGAGTTGTGCACGTTTTCTCAAGTCTGGGTTTTTAGCGATACACCTTCTCGTCTGCCGGAACt                                       <  2:3520507/98‑1 (MQ=255)
                                                           tGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtga                                     >  2:1710292/1‑100 (MQ=255)
                                                           tGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtga                                     <  2:3558878/100‑1 (MQ=255)
                                                           tGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtga                                     <  2:2053756/100‑1 (MQ=255)
                                                           tGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtga                                     <  2:332963/100‑1 (MQ=255)
                                                            gAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtgat                                    <  1:3079667/100‑1 (MQ=255)
                                                            gAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtgat                                    <  1:3937494/100‑1 (MQ=255)
                                                             aaaaTCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACtgatg                                   <  2:1531028/100‑1 (MQ=255)
                                                              aaaTCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCCCGTCTGCCGGAACtgatga                                  >  2:800483/1‑100 (MQ=255)
                                                                aTCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAAc                                <  1:2737448/100‑1 (MQ=255)
                                                                 tCTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACg                               >  1:2134795/1‑100 (MQ=255)
                                                                  cTTCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACgg                              >  1:2701103/1‑100 (MQ=255)
                                                                   ttCGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGt                             >  2:1234851/1‑100 (MQ=255)
                                                                     cGCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTgc                           <  1:1507954/100‑1 (MQ=255)
                                                                      gCAAAGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTgcg                          >  1:1099554/1‑100 (MQ=255)
                                                                          aGTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAgg                      <  1:3100236/100‑1 (MQ=255)
                                                                           gTCATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGc                     <  2:2414200/100‑1 (MQ=255)
                                                                            tcgtttAATTAAAGGGTATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGCTGAACGGTGCGCAGGCt                    <  2:1607107/97‑1 (MQ=255)
                                                                             cATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCtt                   >  2:1876771/1‑100 (MQ=255)
                                                                             cATTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCtt                   >  1:1318147/1‑100 (MQ=255)
                                                                              aTTTAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCttt                  >  1:1214547/1‑100 (MQ=255)
                                                                               tttAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTa                 <  2:1082218/100‑1 (MQ=255)
                                                                                 tAATTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGc               <  1:1655649/100‑1 (MQ=255)
                                                                                  aaTTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCt              <  1:1228831/100‑1 (MQ=255)
                                                                                   aTTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTa             <  2:464215/100‑1 (MQ=255)
                                                                                   aTTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTa             <  2:613149/100‑1 (MQ=255)
                                                                                   aTTACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTa             <  1:3640454/100‑1 (MQ=255)
                                                                                    ttACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaa            <  1:1920459/100‑1 (MQ=255)
                                                                                     tACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaat           <  2:2780018/100‑1 (MQ=255)
                                                                                     tACAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCAATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTAAGCTaat           >  2:3716717/1‑100 (MQ=255)
                                                                                      aCAGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaatc          >  2:2858625/1‑100 (MQ=255)
                                                                                        aGGGGATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaatcaa        <  1:2951893/100‑1 (MQ=255)
                                                                                           ggATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaatcaaatc     >  2:1279734/1‑100 (MQ=255)
                                                                                           ggATGCGATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTaatcaaatc     <  2:3416719/100‑1 (MQ=255)
                                                                                             aTGCGATCAACACTTTTTCTCAAGTCTGGGTATTCAGCCATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAatctaatcaa   <  1:3405392/100‑1 (MQ=255)
                                                                                              tGCGATGAACACGTTTCCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAac  <  2:2231530/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGCGAACGTCAGCGCATCGTGATTGAAGGCGACGGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAATTACAGGGGATGCTATGAACACGTTTTCTCAAGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCGGAACTGATGAACGGTGCGCAGGCTTTAGCTAATCAAATCAAC  >  NC_000913/43090‑43283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: