Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 832,388 T→G intergenic (+152/+80) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3880TG93.7% 516.0 / 13.4 175intergenic (+152/+80)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base G (83/81);  total (88/87)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 176 total aligned reads displayed.

CTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAT  >  NC_000913/832291‑832486
                                                                                                 |                                                                                                  
cTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAgtt                                                                                                  >  1:1005811/1‑100 (MQ=255)
 tGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTc                                                                                                 <  1:2988738/100‑1 (MQ=255)
   aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTcac                                                                                               <  2:1268696/100‑1 (MQ=255)
   aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTcac                                                                                               <  1:142296/100‑1 (MQ=255)
    tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTcaca                                                                                              <  1:3862005/100‑1 (MQ=255)
    tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTcaca                                                                                              <  2:563409/100‑1 (MQ=255)
      cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCacaac                                                                                            <  1:721847/100‑1 (MQ=255)
      cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCacaac                                                                                            >  2:1633580/1‑100 (MQ=255)
         tACGCTTATCAGGCCTACGTGAACTCTGCAATATATTTAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAg                                                                                         >  2:1937420/1‑100 (MQ=255)
         tACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAg                                                                                         <  1:2092068/100‑1 (MQ=255)
            gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCAc                                                                                      <  2:3582526/100‑1 (MQ=255)
            gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCAc                                                                                      <  2:264101/100‑1 (MQ=255)
              ttATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACtt                                                                                    >  2:763715/1‑100 (MQ=255)
               tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTg                                                                                   >  2:1362342/1‑100 (MQ=255)
               tATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTg                                                                                   >  2:3581773/1‑100 (MQ=255)
                aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGt                                                                                  >  1:3531690/1‑100 (MQ=255)
                 tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTc                                                                                 >  1:1955600/1‑100 (MQ=255)
                 tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTc                                                                                 <  2:2904392/100‑1 (MQ=255)
                 tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTc                                                                                 >  1:2686178/1‑100 (MQ=255)
                  cAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCa                                                                                >  2:1439722/1‑100 (MQ=255)
                  cAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCa                                                                                <  1:3273234/100‑1 (MQ=255)
                   aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAg                                                                               >  1:518232/1‑100 (MQ=255)
                   aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAg                                                                               >  2:1786624/1‑100 (MQ=255)
                    ggCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGc                                                                              <  2:1733062/100‑1 (MQ=255)
                     gCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCa                                                                             >  1:2766333/1‑100 (MQ=255)
                        tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAAtt                                                                          <  1:2906399/100‑1 (MQ=255)
                         aCGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAATCACTTGTGAGCAAttt                                                                         <  2:3866558/100‑1 (MQ=21)
                          cGTGAACTCTGCAATATATTGAATGTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTg                                                                        <  2:3989838/100‑1 (MQ=255)
                           gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                       <  1:3667498/100‑1 (MQ=255)
                           gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTga                                                                       >  1:1373695/1‑100 (MQ=255)
                           gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTga                                                                       <  1:314973/100‑1 (MQ=255)
                            tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTgag                                                                      <  1:109086/100‑1 (MQ=255)
                             gAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGCCAGCAATTTgaga                                                                     >  2:269158/1‑100 (MQ=255)
                               aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAac                                                                   >  1:1837573/1‑100 (MQ=255)
                                   tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACgg                                                               <  1:3724543/100‑1 (MQ=255)
                                    gCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGATTACGCCGCATCCGTCAGTTCACAACAAGCACTTGTCAGCAATTTCAGAACACggg                                                              >  2:3956625/1‑100 (MQ=25)
                                      aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaa                                                            >  2:142703/1‑100 (MQ=255)
                                              gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTa                                                    <  2:1398020/100‑1 (MQ=255)
                                               aaTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTAt                                                   >  1:452890/1‑100 (MQ=255)
                                                    gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                              <  2:2691232/100‑1 (MQ=255)
                                                    gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                              <  1:2307314/100‑1 (MQ=255)
                                                     cATGCTTTTGTAGTCCTGATAAGGCGCTTACACCACATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATGTATCTGttt                                             >  2:1608587/1‑100 (MQ=255)
                                                     cATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGttt                                             >  2:3284949/1‑100 (MQ=255)
                                                      aTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                            >  1:1307639/1‑100 (MQ=255)
                                                       tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTa                                           >  1:2847637/1‑100 (MQ=255)
                                                       tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGAACTTGTCAGCAATTTGAGAACAAGGGAAAACATCTACCTGTTTTa                                           >  1:3066147/1‑100 (MQ=25)
                                                        tgctttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                          >  2:920223/4‑100 (MQ=255)
                                                        gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                          >  1:933870/1‑100 (MQ=255)
                                                        gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                          <  1:2736442/100‑1 (MQ=255)
                                                         cTTTTGTAGGCCGGATAAGGCGTTTACGCTGCATCCGGCAGTTCACAACAAGCACTTGGCAACAATTTGAGAACACGGGAAAAAATTTATCAGTTTTAcc                                         >  1:3907273/1‑100 (MQ=37)
                                                         cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAcc                                         >  1:3295731/1‑100 (MQ=255)
                                                            ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                      <  2:2961531/100‑1 (MQ=255)
                                                            ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                      <  1:3675179/100‑1 (MQ=255)
                                                             tGTAGGCCGGATAAGGCGTTTAGGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGccc                                     <  2:2608212/100‑1 (MQ=255)
                                                             tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAACATTTATCTGTTTTACCGccc                                     >  2:2904083/1‑100 (MQ=255)
                                                             tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGccc                                     >  1:226653/1‑100 (MQ=255)
                                                              gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCa                                    <  1:3510001/100‑1 (MQ=255)
                                                               tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCAt                                   <  1:2468542/100‑1 (MQ=255)
                                                               tAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCAt                                   <  2:1714080/100‑1 (MQ=255)
                                                                aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATa                                  >  1:2213083/1‑100 (MQ=255)
                                                                 ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                 >  2:1564466/1‑100 (MQ=255)
                                                                  gCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACTGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                <  1:3589957/100‑1 (MQ=255)
                                                                  gCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                >  1:2843932/1‑100 (MQ=255)
                                                                  gCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                <  1:2371044/100‑1 (MQ=255)
                                                                  gCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                <  1:3488752/100‑1 (MQ=255)
                                                                  gCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                >  1:824455/1‑100 (MQ=255)
                                                                   ccGGATAAGGCGTTTACGCCGCATGCGGCAGTTCACAACGAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                               <  2:823337/100‑1 (MQ=255)
                                                                   ccGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                               >  2:2010378/1‑100 (MQ=255)
                                                                   ccGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGc                               >  1:583804/1‑100 (MQ=255)
                                                                    cGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTTAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGcc                              <  2:3620503/100‑1 (MQ=255)
                                                                      gATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCAc                            >  2:740947/1‑100 (MQ=255)
                                                                        tAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCg                          <  1:916697/100‑1 (MQ=255)
                                                                          aGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgag                        >  1:1766560/1‑100 (MQ=255)
                                                                           ggCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgaga                       >  1:197099/1‑100 (MQ=255)
                                                                           ggCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgaga                       >  2:1119092/1‑100 (MQ=255)
                                                                           ggCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGAGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgaga                       >  1:501823/1‑100 (MQ=255)
                                                                             cGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGACCACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACg                     <  1:4011103/100‑1 (MQ=255)
                                                                             cGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACg                     >  1:2046710/1‑100 (MQ=255)
                                                                             cGTTTACGCCGCATCCGGCAGTTAACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTAACTGTTTTACCGCCCATAAGCCACCGAGACg                     >  2:2735973/1‑100 (MQ=39)
                                                                              gTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGt                    >  2:2805947/1‑100 (MQ=255)
                                                                               tttACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGtt                   <  1:3909834/100‑1 (MQ=255)
                                                                                ttACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTa                  >  1:1920916/1‑100 (MQ=255)
                                                                                  aCGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                <  1:1163389/100‑1 (MQ=255)
                                                                                  aCGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                <  2:2983761/100‑1 (MQ=255)
                                                                                  aCGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                <  2:1315172/100‑1 (MQ=255)
                                                                                  aCGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                <  2:2240053/100‑1 (MQ=255)
                                                                                   cgccgcATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAAGACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCAACCGAGACGTTACtt               >  2:1048341/1‑100 (MQ=255)
                                                                                   cgccgcATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACtt               >  1:1717039/1‑100 (MQ=255)
                                                                                   cgccgcATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACtt               >  2:3428492/1‑100 (MQ=255)
                                                                                    gccgcATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGTCCGTCGGCCACCGAGACGTTACTtc              <  2:725154/100‑1 (MQ=255)
                                                                                     ccgcATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCACAACCCACCGAGCCGTTACTtct             >  2:1944827/1‑100 (MQ=255)
                                                                                     ccgcATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct             >  2:2499111/1‑100 (MQ=255)
                                                                                        cATCCGGCAGTTCACAACAAGCACTTGTCAGTAATTTGAGAACACGTGAAAAAATTTATCTGTTTTCCCGCCCATAAGCCACCGAGACGTTACTTCTCta          <  2:3315814/100‑1 (MQ=255)
                                                                                        cATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCta          <  1:1568034/100‑1 (MQ=255)
                                                                                           ccGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAg       >  2:420561/1‑100 (MQ=255)
                                                                                           ccGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAg       >  1:1223960/1‑100 (MQ=255)
                                                                                            cGGCAGTTCACAAGAAGCACTTGTCAGCAATATGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGc      <  2:3139380/100‑1 (MQ=255)
                                                                                            cGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGc      >  2:2101761/1‑100 (MQ=255)
                                                                                             ggCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc     <  2:2523751/100‑1 (MQ=255)
                                                                                                aTTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAt  <  1:1696898/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
CTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAT  >  NC_000913/832291‑832486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: