BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,341	T→G	S326A (TCT→GCT)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,341	0	T	G	91.7%	362.6 / 17.2	122	S326A (TCT→GCT)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand): ref base T (6/4); new base G (61/50); total (67/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 130 total aligned reads displayed.

GCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTT  >  NC_000913/969245‑969436

  cTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTtgctggctgctggc                                                                                            >  2:3475325/1‑100 (MQ=255)

  cTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTtgctggctgctggc                                                                                            >  2:76907/1‑100 (MQ=255)

  cTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTtgctggctgctggc                                                                                            <  1:1947393/100‑1 (MQ=255)

   tttGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCa                                                                                           >  1:2759081/1‑100 (MQ=255)

     tGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAAc                                                                                         <  1:3684316/100‑1 (MQ=255)

     tGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAAc                                                                                         <  1:1278476/100‑1 (MQ=255)

     tGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAAc                                                                                         <  2:1641731/100‑1 (MQ=255)

      gCAGAAGAAAATTGGCGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACt                                                                                        >  2:323806/1‑100 (MQ=255)

       cAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAAATAAGCTTGCTGGCTGCTGGCAACTa                                                                                       >  2:3790299/1‑100 (MQ=255)

        agaagaAAATTGGTGGTATTTGCCTGTAGACGCATAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAg                                                                                      <  1:2878668/100‑1 (MQ=255)

        agaagaAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAg                                                                                      <  2:3599560/100‑1 (MQ=255)

        agaagaAAATTGGTGGTATTTGCATGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAg                                                                                      <  2:1114457/100‑1 (MQ=255)

         gaagaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGt                                                                                     >  1:3361300/1‑100 (MQ=255)

         gaagaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGt                                                                                     >  2:3165979/1‑100 (MQ=255)

         gaagaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGt                                                                                     <  2:3358850/100‑1 (MQ=255)

           agaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTa                                                                                   >  2:1890245/1‑100 (MQ=255)

           agaaAATTGGTGGTATTCGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTa                                                                                   >  1:3550161/1‑100 (MQ=255)

            gaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTAc                                                                                  >  1:2490362/1‑100 (MQ=255)

            gaaAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTAc                                                                                  <  1:2185102/100‑1 (MQ=255)

              aaaTTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTAcgc                                                                                >  1:1022613/1‑100 (MQ=255)

                    gtggtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGc                                                                          <  1:275151/100‑1 (MQ=255)

                      ggtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAg                                                                        >  1:190554/1‑100 (MQ=255)

                             gCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCgc                                                                 >  1:4082748/1‑99 (MQ=255)

                             gCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGa                                                                 <  1:281151/100‑1 (MQ=255)

                             gCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGa                                                                 >  1:1931988/1‑100 (MQ=255)

                              ccTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTACCCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGAt                                                                <  2:547824/100‑1 (MQ=255)

                              ccTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGAt                                                                <  1:3469185/100‑1 (MQ=255)

                              ccTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGAt                                                                <  1:2678719/100‑1 (MQ=255)

                              ccTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGAt                                                                >  2:1091196/1‑100 (MQ=255)

                              ccTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGAt                                                                <  1:102637/100‑1 (MQ=255)

                                tGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTg                                                              <  1:2416129/100‑1 (MQ=255)

                                tGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTg                                                              <  2:1846496/100‑1 (MQ=255)

                                tGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTg                                                              >  1:3021533/1‑100 (MQ=255)

                                 gTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGa                                                             >  1:1904002/1‑100 (MQ=255)

                                  tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGAt                                                            <  1:576585/100‑1 (MQ=255)

                                     aCGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAAATAGTTACGCCGCGGCAGCGTTCGATTGATGGa                                                         >  1:3584960/1‑100 (MQ=255)

                                      cGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTCGTTACGCCGCGGCAGCGTTCGATTGATGGAg                                                        >  2:1016602/1‑100 (MQ=255)

                                      cGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAg                                                        <  2:164562/100‑1 (MQ=255)

                                      cGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAg                                                        >  2:1835520/1‑100 (MQ=255)

                                       gCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGt                                                       <  2:560848/100‑1 (MQ=255)

                                        cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAGCCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTc                                                      >  1:3578822/1‑100 (MQ=255)

                                        cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTc                                                      >  1:962223/1‑100 (MQ=255)

                                        cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTc                                                      >  1:544099/1‑100 (MQ=255)

                                        cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTc                                                      >  1:1424623/1‑100 (MQ=255)

                                         acaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTAGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCa                                                     >  1:3651955/1‑100 (MQ=255)

                                          caGCTTTCAGGTGGTGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCAt                                                    <  2:2415703/100‑1 (MQ=255)

                                           aGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATg                                                   <  1:1332694/100‑1 (MQ=255)

                                           aGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATg                                                   <  2:2561352/100‑1 (MQ=255)

                                            gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                  >  1:1832224/1‑100 (MQ=255)

                                            gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                  >  2:664891/1‑100 (MQ=255)

                                            gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                  >  2:2496266/1‑100 (MQ=255)

                                            gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                  >  2:1345028/1‑100 (MQ=255)

                                              tttCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAAt                                                >  2:2052928/1‑100 (MQ=255)

                                              tttCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAAt                                                <  2:2638854/100‑1 (MQ=255)

                                              tttCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAAt                                                >  1:2729450/1‑100 (MQ=255)

                                               ttCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCCCGGCAGCGTTCGATTGATGGAGTCATGAATg                                               >  2:1690186/1‑100 (MQ=255)

                                                 cAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTc                                             >  1:2039050/1‑100 (MQ=255)

                                                   ggTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGc                                           <  2:3454806/100‑1 (MQ=255)

                                                   ggTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGc                                           <  1:3671510/100‑1 (MQ=255)

                                                    gTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCt                                          <  1:15141/100‑1 (MQ=255)

                                                    gTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCt                                          <  2:2152907/100‑1 (MQ=255)

                                                     tgatgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTg                                         <  1:1353578/100‑1 (MQ=255)

                                                      gatgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGc                                        >  1:1014620/1‑100 (MQ=255)

                                                          aaCCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCACCTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGGCGCa                                    >  2:2919292/1‑100 (MQ=255)

                                                          aaCCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCa                                    <  2:2651313/100‑1 (MQ=255)

                                                          aaCCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCACGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCa                                    >  1:2102379/1‑100 (MQ=255)

                                                           aCCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                   >  1:223650/1‑100 (MQ=255)

                                                            ccAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAcn                                  <  1:3262960/100‑2 (MQ=255)

                                                            ccAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAcc                                  <  2:382737/100‑1 (MQ=255)

                                                             cAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACct                                 >  2:2113565/1‑100 (MQ=255)

                                                              aGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGGATGTCGCTGCCGCACctc                                >  1:2545253/1‑100 (MQ=255)

                                                              aGCGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctc                                >  1:229548/1‑100 (MQ=255)

                                                                cGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGGAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                              >  2:3905381/1‑100 (MQ=255)

                                                                cGAAACTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                              >  2:3120689/1‑100 (MQ=255)

                                                                     cTGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTg                         >  2:1220880/1‑100 (MQ=255)

                                                                      tGCTTACGTAACTAACCTTGCTGGCGGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCGGCCGCACCTCTCCCTTGc                        <  1:1157892/100‑1 (MQ=255)

                                                                      tGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGc                        <  1:1157917/100‑1 (MQ=255)

                                                                      tGCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGc                        <  1:1157933/100‑1 (MQ=255)

                                                                       gCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCt                       >  1:3829543/1‑100 (MQ=255)

                                                                       gCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCt                       <  2:1915484/100‑1 (MQ=255)

                                                                       gCTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCt                       >  1:3016357/1‑100 (MQ=255)

                                                                        cTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTg                      <  2:3095238/100‑1 (MQ=255)

                                                                        cTTACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTg                      <  1:1402430/100‑1 (MQ=255)

                                                                          tACGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGAt                    >  1:751250/1‑100 (MQ=255)

                                                                          tACGCAACTAACCTTGCTGGCTGATGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCGCCTCTCCCTTGCTGAt                    >  2:3344464/1‑100 (MQ=255)

                                                                            cGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgc                  >  1:445307/1‑100 (MQ=255)

                                                                            cGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgc                  >  1:1014712/1‑100 (MQ=255)

                                                                            cGCAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgc                  >  1:1269966/1‑100 (MQ=255)

                                                                              cAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgcgc                <  2:2411994/100‑1 (MQ=255)

                                                                              cAACTAACCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATgcgc                >  1:230004/1‑100 (MQ=255)

                                                                                   naCCTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAAt           >  1:3233709/2‑100 (MQ=255)

                                                                                    aCCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATc          >  2:2079785/1‑100 (MQ=255)

                                                                                     ccTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGCTTGATGGAGCCATGAATGTCGCTGCCGCCCCTCTCCCTTGCTGATGCGCGTAATCt         >  2:1581229/1‑100 (MQ=255)

                                                                                     ccTTGCTGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCt         >  2:486338/1‑100 (MQ=255)

                                                                                        tgctggctgctggcAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCa      <  1:491128/100‑1 (MQ=255)

                                                                                        tGCTGGCTCCAGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCa      <  1:187447/100‑1 (MQ=255)

                                                                                         gctggctgCTGGCAACTAGTTACGCCGCGGCAGCGTTAGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGCTGCGCGTAATCTTCAc     >  2:368785/1‑100 (MQ=255)

                                                                                           tGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCt   >  2:2169692/1‑100 (MQ=255)

                                                                                           tGGCTGCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCt   <  2:716368/100‑1 (MQ=255)

                                                                                            ggCTGCTGGCAACTGGTTACGCCGGGGCAGAGTTCGGTTGCTGGAGTCATGAATGTCGCTGCAGAAGCTCTGCATTGCTGCTGAGGGTGATATTTACCtt  >  2:1998330/1‑100 (MQ=255)

GCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTT  >  NC_000913/969245‑969436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`