Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,048,134 A→C N186K (AAT→AAG gfcC ← putative O‑antigen capsule production periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,048,1340AC97.3% 498.7 / ‑0.4 151N186K (AAT→AAGgfcCputative O‑antigen capsule production periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base C (78/68);  total (82/69)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

AAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACC  >  NC_000913/1048038‑1048232
                                                                                                |                                                                                                  
aaGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGttcttc                                                                                                 <  1:2758620/100‑1 (MQ=255)
aaGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGttcttc                                                                                                 <  1:2227347/100‑1 (MQ=255)
 aGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                                <  2:603181/100‑1 (MQ=255)
 aGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATGCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                                <  2:2606101/100‑1 (MQ=255)
 aGCTGGCTGCCAGGCGGTGGTCCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                                <  1:85293/100‑1 (MQ=255)
 aGCTGGCTGCCAGGCGGTAGGTCCACCTGTCTTTTATTCTACAGGCCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTtcttct                                                                                                <  1:3817235/100‑1 (MQ=255)
  gctggctgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCTACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTcttctt                                                                                               >  1:1162476/1‑100 (MQ=255)
        tgCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCcgc                                                                                         <  1:2789403/100‑1 (MQ=255)
           cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGcc                                                                                      <  2:139758/100‑1 (MQ=255)
                ggtggtTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCaa                                                                                 >  1:3585693/1‑100 (MQ=255)
                    gtTCCACGTGTCTTTTGTTCCTCAGTGCATCAGTGTCGACGACAGTCTCACTTTCTGCCTTGATCACGAGAACGTGGGTCGTGTCCGCGCCTGCAAGGCg                                                                             >  2:3996423/1‑100 (MQ=255)
                     ttCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCgg                                                                            >  2:2845488/1‑100 (MQ=255)
                      tCCACATGTCTTTTATTCCACAGGGCAGCAGGGGAGACGAGAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                           <  2:3820598/100‑1 (MQ=255)
                      tCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                           <  2:3500855/100‑1 (MQ=255)
                      tCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggg                                                                           <  1:2237773/100‑1 (MQ=255)
                       ccACGTGTCTTTTATTCCACAGGGCAACCGGGGCGACGACAGTCTCATCATCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCCAGGCgggg                                                                          >  2:230724/1‑100 (MQ=255)
                       ccACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCgggg                                                                          <  1:3505666/100‑1 (MQ=255)
                        cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                         <  1:184548/100‑1 (MQ=255)
                        cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                         >  1:3745692/1‑100 (MQ=255)
                        cacaTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCggggg                                                                         <  1:1047950/100‑1 (MQ=255)
                          caTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTTTCCGCGCCTGCAAGGCGGGGGTg                                                                       >  1:433083/1‑100 (MQ=255)
                          caTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTg                                                                       >  1:2571592/1‑100 (MQ=255)
                           aTGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGa                                                                      >  1:3384786/1‑100 (MQ=255)
                             ntCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                    >  1:3242020/2‑100 (MQ=255)
                             gTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                    >  1:3610849/1‑100 (MQ=255)
                             gTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                    >  2:2186024/1‑100 (MQ=255)
                              tCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCt                                                                   >  2:2554138/1‑100 (MQ=255)
                              tCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCt                                                                   <  2:462181/100‑1 (MQ=255)
                               cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                  >  2:2081842/1‑100 (MQ=255)
                               cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                  >  1:3440175/1‑100 (MQ=255)
                               cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCtt                                                                  >  1:1143994/1‑100 (MQ=255)
                               cTTTTAGTCCACAGGACAACAGGGGCGACACCAGTGTCACCCTCAGGCGTAGTAAACATAACGGTATTCTTATACGCGCCTGCAACGAGGGCGGGATCta                                                                  >  2:1865839/1‑99 (MQ=255)
                                 tttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGc                                                                <  1:753776/100‑1 (MQ=255)
                                 tttATTCCACAGGGAAAAAGGGGAGACGACAGTCTCACCCTAAGGAATACACACCATAAACTCCTTCTTATACGCGCCTGAAAGGAGAGGGTGATGttaa                                                                >  2:3019549/1‑98 (MQ=255)
                                  ttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCa                                                               <  1:126598/100‑1 (MQ=255)
                                  ttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCa                                                               <  2:3293075/100‑1 (MQ=255)
                                  ttATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCa                                                               <  1:727877/100‑1 (MQ=255)
                                    aTTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGa                                                             <  2:3860181/100‑1 (MQ=255)
                                    aTTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGa                                                             >  2:3171674/1‑100 (MQ=255)
                                    aTTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGa                                                             >  1:877370/1‑100 (MQ=255)
                                    aTTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGa                                                             <  1:3592321/100‑1 (MQ=255)
                                       ccACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATaa                                                          >  2:2670518/1‑100 (MQ=255)
                                       ccACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGCTGATCTTGCAGATaa                                                          >  2:390155/1‑100 (MQ=255)
                                         acaGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATc                                                        <  1:2943527/100‑1 (MQ=255)
                                          caGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCg                                                       <  2:1152613/100‑1 (MQ=255)
                                           aGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCgg                                                      <  1:2134050/100‑1 (MQ=255)
                                            gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                     >  1:1690186/1‑100 (MQ=255)
                                            gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                     <  1:1216760/100‑1 (MQ=255)
                                            gggCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                     >  1:2081966/1‑100 (MQ=255)
                                              gCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTaa                                                   <  2:216537/100‑1 (MQ=255)
                                              gCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTaa                                                   >  1:2669471/1‑100 (MQ=255)
                                               cAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAAc                                                  <  1:1612704/100‑1 (MQ=255)
                                               cAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAAc                                                  <  1:3203868/100‑1 (MQ=255)
                                                aaCAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACg                                                 <  2:354222/100‑1 (MQ=255)
                                                    ggggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTAc                                             >  1:2445716/1‑100 (MQ=255)
                                                      ggCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACgg                                           >  1:2822576/1‑100 (MQ=255)
                                                        cgacgaCAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcc                                         >  2:677641/1‑100 (MQ=255)
                                                        cgacgaCAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcc                                         >  1:633681/1‑100 (MQ=255)
                                                         gacgacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcct                                        >  1:737154/1‑100 (MQ=255)
                                                            gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                     <  2:246270/100‑1 (MQ=255)
                                                            gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                     <  2:1208704/100‑1 (MQ=255)
                                                            gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                     >  1:1743117/1‑100 (MQ=255)
                                                            gacAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcc                                     >  2:847718/1‑100 (MQ=255)
                                                             acAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctgcct                                    >  2:2868053/1‑100 (MQ=255)
                                                              cAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGcctgcctg                                   <  2:1341147/100‑1 (MQ=255)
                                                              cAGTCTCACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGcctgcctg                                   >  2:1935071/1‑100 (MQ=255)
                                                                gTCTCACCCTCAGGCGTACTCCCCATAACGGTCTTCATGTCCGCGCATGCAGGACGGGCGTGACCTCGCGCATAACCGGTAACCCTACGccctcccgcac                                 >  2:3103285/1‑96 (MQ=255)
                                                                  ctcACCCTCAGGCGTGATCCGCATAACGTTCTTCTTGTCCGCGGCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAGCGCTACGGCCTGGCTGCCAg                               <  2:602336/100‑1 (MQ=255)
                                                                  ctcACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAg                               <  2:2512131/100‑1 (MQ=255)
                                                                  ctcACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAg                               <  2:2927308/100‑1 (MQ=255)
                                                                  ctcACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTACAGATAATCGGTAACGCTACGGCCTGCCTGCCAg                               <  2:3699152/100‑1 (MQ=255)
                                                                    cACCCTGAGGCGTAATCACCATAACGTTCTGCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTTCTAggg                             <  2:3763212/100‑1 (MQ=255)
                                                                    cACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAggg                             >  1:1111183/1‑100 (MQ=255)
                                                                    cACCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAggg                             >  1:504026/1‑100 (MQ=255)
                                                                    cACCCTCAGGCGTAATCACCATAACGTTCTGCTTGTCTGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCCGTAACGCTCCGGCCTGCCTGCCAgtg                             >  2:2766824/1‑98 (MQ=255)
                                                                     aCCCTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGc                            <  1:1797494/100‑1 (MQ=255)
                                                                        cTCAGGCGTAATCTCCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAAt                         <  2:3130270/100‑1 (MQ=255)
                                                                        cTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCaac                         >  2:1488324/1‑99 (MQ=255)
                                                                        cTCAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAAt                         >  1:2947061/1‑100 (MQ=255)
                                                                          cAGGCGTAATCCCCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTg                       <  2:359252/100‑1 (MQ=255)
                                                                          cAGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTg                       >  2:475656/1‑100 (MQ=255)
                                                                           aGGCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGc                      <  2:726906/100‑1 (MQ=255)
                                                                            ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                     >  1:1909653/1‑100 (MQ=255)
                                                                            ggCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGCAATTGccc                    <  2:3764640/100‑1 (MQ=255)
                                                                             gCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGccc                    <  2:1121510/100‑1 (MQ=255)
                                                                             gCGTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGccc                    <  2:779129/100‑1 (MQ=255)
                                                                               gTAATCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCgg                  <  1:3170317/100‑1 (MQ=255)
                                                                                 aaTCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcc                >  2:1344465/1‑100 (MQ=255)
                                                                                 aaTCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcc                <  2:1742348/100‑1 (MQ=255)
                                                                                  aTCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGccc               >  2:1371079/1‑100 (MQ=255)
                                                                                   tCACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcccc              >  1:571958/1‑100 (MQ=255)
                                                                                    cACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa             >  1:1263268/1‑100 (MQ=255)
                                                                                    cACCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCa             >  2:1716056/1‑100 (MQ=255)
                                                                                     aCCATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCGCCAg            >  2:2531906/1‑100 (MQ=255)
                                                                                      ccATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGa           >  2:2394130/1‑100 (MQ=255)
                                                                                      ccATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGCGATCTTGGAGATAATCGGTAACGCTACGGCCTGCCTGCCAGCGCAATTGCCCGGCCCCAGa           >  1:3620432/1‑100 (MQ=255)
                                                                                       cATAACGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGaa          <  2:1139863/100‑1 (MQ=255)
                                                                                            cGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGc     >  2:2958114/1‑100 (MQ=255)
                                                                                            cGTTCTTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGc     <  2:2908089/100‑1 (MQ=255)
                                                                                               tATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc  >  2:3732640/1‑100 (MQ=255)
                                                                                                |                                                                                                  
AAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACC  >  NC_000913/1048038‑1048232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: