Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,539 T→G E254A (GAA→GCA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,5390TG98.8% 574.5 / ‑1.9 166E254A (GAA→GCA) sgrRtranscriptional DNA‑binding transcriptional activator of sgrS sRNA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (78/86);  total (79/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 166 total aligned reads displayed.

GAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCG  >  NC_000913/76442‑76635
                                                                                                 |                                                                                                
gAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGcg                                                                                                >  1:1328834/1‑100 (MQ=255)
     ccTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCgg                                                                                           >  1:3854511/1‑100 (MQ=255)
      ctaaaTTCTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGc                                                                                          <  2:242827/97‑1 (MQ=255)
      cTGAAGTTTCAGTTGATGGGTGCGGTTGCGAATCACGGCATGCGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGc                                                                                          >  2:805995/1‑100 (MQ=255)
       tGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCa                                                                                         <  1:3693584/100‑1 (MQ=255)
       tGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCa                                                                                         <  1:247104/100‑1 (MQ=255)
        gAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCGAAGTTACTGAGGGTTTCCCATGCGCGCGGCAg                                                                                        <  1:957234/100‑1 (MQ=255)
        gAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAg                                                                                        <  2:2142365/100‑1 (MQ=255)
         aaTTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAgg                                                                                       <  1:2760301/100‑1 (MQ=255)
          aTTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGa                                                                                      <  2:3616591/100‑1 (MQ=255)
          aTTTTCAGTTGATTGGTGCTGTTACGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGa                                                                                      >  1:2760895/1‑100 (MQ=255)
           ttttCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGat                                                                                     >  1:1592083/1‑100 (MQ=255)
           ttgtCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGat                                                                                     >  1:613051/4‑100 (MQ=255)
            tttCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatc                                                                                    <  2:666186/100‑1 (MQ=255)
             ttCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGatca                                                                                   >  2:347572/1‑100 (MQ=255)
               cAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatcatc                                                                                 >  1:1352763/1‑100 (MQ=255)
                aGTTGATTTGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCg                                                                                <  1:1081946/100‑1 (MQ=255)
                  ttGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGcc                                                                              <  1:1549856/100‑1 (MQ=255)
                       tGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAAc                                                                         >  1:1428003/1‑100 (MQ=255)
                       tGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAAc                                                                         >  1:1175149/1‑100 (MQ=255)
                        ggTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACt                                                                        <  2:2927557/100‑1 (MQ=255)
                          tGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTg                                                                      >  1:568358/1‑100 (MQ=255)
                             tgtgGCGAATCACCGCCTACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGCGGTTCCCATGCGCGCGGCAGGAGCATCGCCGGAACTTGccc                                                                   <  2:3075438/100‑1 (MQ=255)
                             tGTTGCGAATCACCGCATACGGACCGGTGCCGATCGGATGGCTGGCAAACTTACTGAGGGTTTCCCATGCGCGCGGCCGGATCATCGCCGGAACTTGccc                                                                   >  2:3697247/1‑100 (MQ=255)
                              gTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGcccc                                                                  >  2:1671829/1‑100 (MQ=255)
                               cggCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCca                                                                 <  1:1023276/98‑1 (MQ=255)
                                tGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcag                                                                <  1:736342/100‑1 (MQ=255)
                                  cGAATCACCGCATACGGACCGGTGCTGGTGGGATGGCTGGCAAAGTTACTGAGGGTTTCCTATGCGCGCGGCAGGATCCTCGCCGGAACTTGCCCcagca                                                              <  2:1549900/100‑1 (MQ=255)
                                   gAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcag                                                             <  2:2031165/100‑1 (MQ=255)
                                   gAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcag                                                             <  1:1213578/100‑1 (MQ=255)
                                       cACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAAc                                                         <  1:2836786/100‑1 (MQ=255)
                                       cACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAAc                                                         <  2:2777675/100‑1 (MQ=255)
                                        aCCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACg                                                        >  1:1880331/1‑100 (MQ=255)
                                        aCCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACg                                                        <  1:4026100/100‑1 (MQ=255)
                                         ccGCATACGGACCGGTGCCGATGGGATGGCTGGCAAATTTACTGAGGGTTCCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACgg                                                       <  2:3838167/100‑1 (MQ=255)
                                         ccGCATACGGACCGGGGCCGATGGGCTGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCGGCAGTAACgg                                                       <  2:2204151/100‑1 (MQ=255)
                                           gCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTa                                                     >  1:3613419/1‑100 (MQ=255)
                                              tACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAAcc                                                  <  1:1849329/100‑1 (MQ=255)
                                              tACGGACCAGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAAcc                                                  <  2:1430791/100‑1 (MQ=255)
                                               aCGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGGTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCa                                                 >  2:2581467/1‑100 (MQ=255)
                                                cGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAg                                                >  1:3720689/1‑100 (MQ=255)
                                                 ggACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGc                                               >  1:2864342/1‑100 (MQ=255)
                                                 ggACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGc                                               <  1:2005137/100‑1 (MQ=255)
                                                 ggACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGc                                               <  1:2897318/100‑1 (MQ=255)
                                                  gACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCg                                              <  1:2010815/100‑1 (MQ=255)
                                                   aCCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCgg                                             <  1:2971316/100‑1 (MQ=255)
                                                     cGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTc                                           <  2:847510/100‑1 (MQ=255)
                                                      ggTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTcc                                          >  1:1415780/1‑100 (MQ=255)
                                                      ggTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTcc                                          >  1:535071/1‑100 (MQ=255)
                                                      ggTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTcc                                          >  1:1169087/1‑100 (MQ=255)
                                                       gTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCg                                         <  1:3102033/100‑1 (MQ=255)
                                                        tGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCCGCAGTAACGGTAACCAGCGGTCCgg                                        >  2:2583988/1‑100 (MQ=255)
                                                        tGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCgg                                        <  2:2821218/100‑1 (MQ=255)
                                                         gCCGATGGGATGGCTTGCAAAGTTACAGAGGGTTTCCCATGCGCGCGGCAGGCACATCGCCGGAACTGGCCTCACCCGTAACGCTAACCACCGGTCCgct                                       >  2:1377260/1‑98 (MQ=255)
                                                          ccGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGtt                                      >  2:3588489/1‑100 (MQ=255)
                                                           cGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCGTCGCCGGAGCTTGCCCCAGCAGTAACGGTAACCAGCGGGCCGGtta                                     >  2:1334435/1‑99 (MQ=255)
                                                           cGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTg                                     >  2:78679/1‑100 (MQ=255)
                                                            gatgggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTgt                                    >  1:3525682/1‑99 (MQ=255)
                                                            gatgggatggCTGGCAAAGTGACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGc                                    <  1:80045/100‑1 (MQ=255)
                                                             atgggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCg                                   <  2:519789/100‑1 (MQ=255)
                                                              tgggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGt                                  >  1:2675217/1‑100 (MQ=255)
                                                              tgggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGt                                  <  1:931394/100‑1 (MQ=255)
                                                                ggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTga                                >  2:2105484/1‑100 (MQ=255)
                                                                 gatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTgag                               <  1:740352/100‑1 (MQ=255)
                                                                 gatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTgag                               <  2:3773540/100‑1 (MQ=255)
                                                                  atggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTgaga                              >  2:613214/1‑100 (MQ=255)
                                                                     gctggcAAAGTTACTGAGGGTTTCCCATGCTCGCGGCAGGATCATCGCTGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATgg                           <  1:3886215/100‑1 (MQ=255)
                                                                      ctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGa                          <  2:2760256/100‑1 (MQ=255)
                                                                      ctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGa                          >  1:2636143/1‑100 (MQ=255)
                                                                      ctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGa                          >  1:828838/1‑100 (MQ=255)
                                                                       tggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGat                         >  2:1632498/1‑100 (MQ=255)
                                                                       tggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGat                         >  1:1729834/1‑100 (MQ=255)
                                                                       tggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGat                         <  1:1463848/100‑1 (MQ=255)
                                                                        ggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGata                        <  2:2302279/100‑1 (MQ=255)
                                                                        ggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGata                        >  2:916507/1‑100 (MQ=255)
                                                                        ggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGata                        <  1:428800/100‑1 (MQ=255)
                                                                           aaaGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATcc                     >  2:843749/1‑100 (MQ=255)
                                                                            aaGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCa                    >  2:768380/1‑100 (MQ=255)
                                                                            aaGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCa                    >  1:2277515/1‑100 (MQ=255)
                                                                              gTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGCCCGGTTGCGTGAGAGGGATATCCAGc                  >  2:1118664/1‑100 (MQ=255)
                                                                               ttACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCg                 <  1:2738763/100‑1 (MQ=255)
                                                                               ttACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCg                 <  1:313735/100‑1 (MQ=255)
                                                                               ttACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCg                 >  1:46294/1‑100 (MQ=255)
                                                                                tACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGt                >  2:2906744/1‑100 (MQ=255)
                                                                                tACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGt                <  1:2061748/100‑1 (MQ=255)
                                                                                  cTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTcc              <  2:2992796/100‑1 (MQ=255)
                                                                                  cTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTcc              >  2:1764564/1‑100 (MQ=255)
                                                                                   tGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCa             <  2:732653/100‑1 (MQ=255)
                                                                                    gAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAg            <  1:1301177/100‑1 (MQ=255)
                                                                                     aGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAgg           >  1:2180032/1‑100 (MQ=255)
                                                                                     aGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAgg           <  1:3920812/100‑1 (MQ=255)
                                                                                     aGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAgg           <  2:2632637/100‑1 (MQ=255)
                                                                                      gggTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAggg          >  2:243361/1‑100 (MQ=255)
                                                                                      gggTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGGCCAggg          >  2:1360246/1‑100 (MQ=255)
                                                                                        gTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCg        >  1:2132546/1‑100 (MQ=255)
                                                                                        gTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCg        <  2:3087128/100‑1 (MQ=255)
                                                                                        gTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCg        <  1:3551392/100‑1 (MQ=255)
                                                                                            nccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCgg    >  1:3241152/2‑100 (MQ=255)
                                                                                            cccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCgg    >  1:689856/1‑100 (MQ=255)
                                                                                             ccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGc   <  2:1490556/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCG  >  NC_000913/76442‑76635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: