BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,139,916	T→G	D513E (GAT→GAG)	flgK →	flagellar hook‑filament junction protein 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,139,916	0	T	G	100.0%	354.5 / NA	100	D513E (GAT→GAG)	flgK	flagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (48/52); total (48/52)

Only 100 of 113 total aligned reads displayed.

TAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTA  >  NC_000913/1139820‑1140010

taaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                             <  1:669005/100‑1 (MQ=255)

taaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                             <  2:719205/100‑1 (MQ=255)

taaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                             >  1:3999048/1‑100 (MQ=255)

taaAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                             >  2:58196/1‑100 (MQ=255)

taaAACCGCGACGTTGAAAACCAGTAGCCCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggag                                                                                             >  1:3598557/1‑100 (MQ=255)

 aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                            >  2:3175020/1‑100 (MQ=255)

 aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                            <  2:1802641/100‑1 (MQ=255)

 aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                            <  1:1718790/100‑1 (MQ=255)

 aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                            <  1:719040/100‑1 (MQ=255)

 aaaaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGGGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGaggagg                                                                                            >  2:704593/1‑100 (MQ=255)

   aaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggaggag                                                                                          <  1:1943460/100‑1 (MQ=255)

   aaCCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCgaggaggag                                                                                          <  1:1243348/100‑1 (MQ=255)

     ccGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTa                                                                                        >  2:1151531/1‑100 (MQ=255)

     ccGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTa                                                                                        <  1:1973845/100‑1 (MQ=255)

     ccGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTa                                                                                        >  1:3430399/1‑100 (MQ=255)

        cgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACgg                                                                                     <  1:2731678/100‑1 (MQ=255)

        cgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACgg                                                                                     <  2:1825296/100‑1 (MQ=255)

        cgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACgg                                                                                     <  2:3760722/100‑1 (MQ=255)

            gTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAAt                                                                                 >  1:2246577/1‑100 (MQ=255)

             ttGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATc                                                                                <  1:3837890/100‑1 (MQ=255)

             ttGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATc                                                                                >  1:2866537/1‑100 (MQ=255)

             ttGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATc                                                                                >  2:2559104/1‑100 (MQ=255)

              tGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCt                                                                               >  1:2812349/1‑100 (MQ=255)

              tGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCt                                                                               <  1:2798044/100‑1 (MQ=255)

                aaaaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGc                                                                             >  1:2050889/1‑100 (MQ=255)

                 aaaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                            <  1:4054469/100‑1 (MQ=255)

                 aaaCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCa                                                                            <  1:1293080/100‑1 (MQ=255)

                   aCCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAAc                                                                          <  1:2296081/100‑1 (MQ=255)

                    ccAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACg                                                                         <  2:3237797/100‑1 (MQ=255)

                    ccAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACg                                                                         >  1:1157574/1‑100 (MQ=255)

                    ccAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACg                                                                         >  1:1291160/1‑100 (MQ=255)

                       gTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGttt                                                                      >  1:1667561/1‑100 (MQ=255)

                       gTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGttt                                                                      >  2:1035759/1‑100 (MQ=255)

                         aGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                    <  2:1800752/100‑1 (MQ=255)

                         aGCGCCACGCAAGGTAAGGTGGTGACGCAGCTGTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTc                                                                    <  2:476860/100‑1 (MQ=255)

                           cgcCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcag                                                                  <  2:2788034/100‑1 (MQ=255)

                            gcCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcagc                                                                 <  1:1174228/100‑1 (MQ=255)

                            gcCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcagc                                                                 <  2:1312780/100‑1 (MQ=255)

                              cACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTcagcag                                                               <  1:3687098/100‑1 (MQ=255)

                                 gCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTAt                                                            <  1:3942815/100‑1 (MQ=255)

                                    aGGTAATATGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTGCCGGGGTCACTCTCGAGAAGACGACCGGAAATCTGCAACGTATTCAGCAGTATTAc                                                         >  2:3859590/1‑100 (MQ=255)

                                      gTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCt                                                       >  2:2713051/1‑100 (MQ=255)

                                       tAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTg                                                      <  1:606611/100‑1 (MQ=255)

                                       tAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTg                                                      <  2:56114/100‑1 (MQ=255)

                                       tAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTg                                                      >  1:73022/1‑100 (MQ=255)

                                            tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGaa                                                 >  1:1275444/1‑100 (MQ=255)

                                            tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGaa                                                 >  2:1977045/1‑100 (MQ=255)

                                            tggtgACGCAGCTTTACAATCAGCAGCAGTCGAGTTCCCGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTCTTACCTGGCGaa                                                 <  2:3846955/100‑1 (MQ=255)

                                             ggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAAt                                                >  1:2687919/1‑100 (MQ=255)

                                             ggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAAt                                                >  1:3293884/1‑100 (MQ=255)

                                              gtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATg                                               <  2:2982275/100‑1 (MQ=255)

                                              gtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATg                                               >  1:2121939/1‑100 (MQ=255)

                                               tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgc                                              <  2:635447/100‑1 (MQ=255)

                                               tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgc                                              <  2:1974938/100‑1 (MQ=255)

                                                gACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgcg                                             >  2:535646/1‑100 (MQ=255)

                                                 aCGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgcgc                                            >  1:177632/1‑100 (MQ=255)

                                                  cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                           >  1:496998/1‑100 (MQ=255)

                                                  cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                           <  2:3429215/100‑1 (MQ=255)

                                                  cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCa                                           <  1:2805871/100‑1 (MQ=255)

                                                   gCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAg                                          <  1:762322/100‑1 (MQ=255)

                                                    cAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAgg                                         <  1:2935896/100‑1 (MQ=255)

                                                       cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTc                                      >  2:2506858/1‑100 (MQ=255)

                                                       cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTc                                      <  1:546549/100‑1 (MQ=255)

                                                          tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                   <  1:3022339/100‑1 (MQ=255)

                                                          tCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGc                                   <  1:2499386/100‑1 (MQ=255)

                                                           ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                  >  1:2727232/1‑100 (MQ=255)

                                                           ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                  >  1:2993525/1‑100 (MQ=255)

                                                           ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                  >  2:2038605/1‑100 (MQ=255)

                                                           ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                  <  2:606868/100‑1 (MQ=255)

                                                           ccAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTACAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCa                                  >  2:90349/1‑100 (MQ=255)

                                                            cAATCAGCAGCAGTCGATTTCCGTTTTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                 <  2:2183427/100‑1 (MQ=255)

                                                            cAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                 <  1:251457/100‑1 (MQ=255)

                                                            cAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                 >  1:2681342/1‑100 (MQ=255)

                                                            cAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAg                                 >  1:3995369/1‑100 (MQ=255)

                                                              aTCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGAc                               >  2:609730/1‑100 (MQ=255)

                                                               tCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACg                              <  2:918620/100‑1 (MQ=255)

                                                               tCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACg                              >  1:2125257/1‑100 (MQ=255)

                                                               tCAGCAGCAGTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACg                              <  2:352215/100‑1 (MQ=255)

                                                                cagcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACgg                             <  1:2567373/100‑1 (MQ=255)

                                                                  gcagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCa                           <  1:2436067/100‑1 (MQ=255)

                                                                   cagcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCaa                          >  2:2871887/1‑100 (MQ=255)

                                                                     gcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAc                        >  1:1231253/1‑100 (MQ=255)

                                                                     gcagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAc                        >  2:2189969/1‑100 (MQ=255)

                                                                      cagTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAcg                       >  1:2301858/1‑100 (MQ=255)

                                                                       agTCGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAAcgc                      >  2:1926949/1‑100 (MQ=255)

                                                                          cGATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAt                   >  2:1763423/1‑100 (MQ=255)

                                                                           ggtttCCGGTGTCAATCACGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAtt                  <  2:2215678/98‑1 (MQ=255)

                                                                           gATTTCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAtt                  <  2:2216185/100‑1 (MQ=255)

                                                                              ttCCGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGAttttt               <  2:3315587/100‑1 (MQ=255)

                                                                                ccGGTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGa             >  2:3432185/1‑100 (MQ=255)

                                                                                  ggTGTCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg           <  2:2092408/100‑1 (MQ=255)

                                                                                    tgtCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg         >  1:735678/1‑100 (MQ=255)

                                                                                     gtCAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcgc        >  2:496427/1‑100 (MQ=255)

                                                                                       cAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACGTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTg      >  1:3773084/1‑100 (MQ=255)

                                                                                       cAATCTCGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTg      <  2:329905/100‑1 (MQ=255)

                                                                                         ttctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCNCAGGNTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAt    <  1:2784451/99‑1 (MQ=255)

                                                                                         aTCACGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAt    >  1:3134828/1‑100 (MQ=255)

                                                                                          tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt   >  2:1504531/1‑100 (MQ=255)

                                                                                          tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt   <  2:2839617/100‑1 (MQ=255)

                                                                                          tctcGAGGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCCATTTTTGATGCGCTGAtt   <  2:3972134/100‑1 (MQ=255)

TAAAACCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTA  >  NC_000913/1139820‑1140010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`