Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,156,452 T→G S231A (TCG→GCG)  holB → DNA polymerase III, delta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,156,4520TG95.3% 341.0 / 5.4 107S231A (TCG→GCG) holBDNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (48/54);  total (50/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 108 total aligned reads displayed.

CCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGTGCTGC  >  NC_000913/1156353‑1156549
                                                                                                   |                                                                                                 
ccTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTAtt                                                                                                   >  1:2782317/1‑100 (MQ=255)
    gcgcGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgct                                                                                               >  1:470355/1‑100 (MQ=255)
     cgcgGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctg                                                                                              <  2:2124954/100‑1 (MQ=255)
      gcgGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctgc                                                                                             <  1:1478795/100‑1 (MQ=255)
       cgGCACTTTCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctgct                                                                                            <  1:1631984/100‑1 (MQ=255)
         gCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAg                                                                                          <  2:3575653/100‑1 (MQ=255)
          cACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGc                                                                                         >  1:46003/1‑100 (MQ=255)
             tGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGc                                                                                      >  2:2429659/1‑100 (MQ=255)
             tGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGc                                                                                      <  1:413146/100‑1 (MQ=255)
              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGcc                                                                                     <  1:2555171/100‑1 (MQ=255)
              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGcc                                                                                     <  1:1270622/100‑1 (MQ=255)
              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGCTATGCGCTGCTCGCGGcc                                                                                     <  1:3403498/100‑1 (MQ=255)
               gCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGccc                                                                                    <  1:3219471/100‑1 (MQ=255)
               gCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGccc                                                                                    <  2:359599/100‑1 (MQ=255)
                cGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCt                                                                                   <  1:817105/100‑1 (MQ=255)
                cGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCt                                                                                   <  1:817118/100‑1 (MQ=255)
                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCtt                                                                                  >  1:2681655/1‑100 (MQ=255)
                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCtt                                                                                  <  1:262060/100‑1 (MQ=255)
                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGACCtt                                                                                  <  2:3384488/100‑1 (MQ=255)
                   tgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTaa                                                                                <  1:992709/100‑1 (MQ=255)
                   tgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTaa                                                                                <  2:2756393/100‑1 (MQ=255)
                    gttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAAt                                                                               <  2:3166267/100‑1 (MQ=255)
                     tttCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATc                                                                              >  1:1595484/1‑100 (MQ=255)
                     tttCAGGGAGATAACGGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATc                                                                              <  1:2085940/100‑1 (MQ=255)
                      ttaaGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGTCGTTGGCATATAGCATGCCATCGGGCGACTGGTATGCGCTGCTAGCAGCCCTTAATCa                                                                             >  2:3704766/4‑100 (MQ=255)
                      ttCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCa                                                                             <  1:3845100/100‑1 (MQ=255)
                        cAGGGAGATGACTGGAAGGCTCGTGAACCTCTGTGTCAGGCGTTGGCATATCGTGTGGAAGCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCTTg                                                                           <  2:620819/100‑1 (MQ=255)
                        cAGGGAGATAACTGGTAGGCTCGTGAGACATTGTGTCAGGTGTTGGCATAGAGCGTGCCATGTGGAGATTGGTGTGCTCGGCGAGCGGTCCTTAATCATg                                                                           >  2:360914/1‑100 (MQ=255)
                        cAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCCTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATg                                                                           >  2:848422/1‑100 (MQ=255)
                          gggAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGaa                                                                         <  1:2453865/100‑1 (MQ=255)
                           ggAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAAc                                                                        <  2:495148/100‑1 (MQ=255)
                              gaTAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAg                                                                     >  1:2986300/1‑100 (MQ=255)
                               aTAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGc                                                                    <  2:2284709/100‑1 (MQ=255)
                                  aCTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTcc                                                                 >  2:1714549/1‑100 (MQ=255)
                                   cTGGCAGTCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCg                                                                >  2:2564914/1‑100 (MQ=255)
                                   cTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGCCTGGTACGCGCTGCTAGCGGCCCTTACTCATGAACAACCTCCg                                                                >  1:3888468/1‑100 (MQ=255)
                                   cTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCg                                                                >  1:637517/1‑100 (MQ=255)
                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  2:3664018/1‑100 (MQ=255)
                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              <  1:2022512/100‑1 (MQ=255)
                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  1:1570660/1‑100 (MQ=255)
                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  1:3388928/1‑100 (MQ=255)
                                        aGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGcgcg                                                           >  1:795271/1‑100 (MQ=255)
                                         ggCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGt                                                          <  2:803598/100‑1 (MQ=255)
                                          gCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGtt                                                         <  2:3550291/100‑1 (MQ=255)
                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttt                                                        <  2:3554968/100‑1 (MQ=255)
                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttt                                                        <  1:1346646/100‑1 (MQ=255)
                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttn                                                        <  1:367528/100‑2 (MQ=255)
                                            tCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTa                                                       <  2:228460/100‑1 (MQ=255)
                                            tCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTa                                                       <  2:1239555/100‑1 (MQ=255)
                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     <  2:1216070/100‑1 (MQ=255)
                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  2:1355297/1‑100 (MQ=255)
                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  1:620654/1‑100 (MQ=255)
                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  1:1770102/1‑100 (MQ=255)
                                               tGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTacac                                                    <  2:778622/100‑1 (MQ=255)
                                               tGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTacac                                                    >  2:3685191/1‑100 (MQ=255)
                                                  aaCATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACActgg                                                 <  1:1526794/100‑1 (MQ=255)
                                                    cATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACActggct                                               >  1:1911138/1‑100 (MQ=255)
                                                       tgtgTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACtggctggc                                            <  2:2941751/100‑1 (MQ=255)
                                                       tgtgTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACtggctggc                                            <  1:1000331/100‑1 (MQ=255)
                                                        gtgtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCa                                           >  1:165093/1‑100 (MQ=255)
                                                          gtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAAc                                         <  2:584520/100‑1 (MQ=255)
                                                          gtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAAc                                         <  1:3764965/100‑1 (MQ=255)
                                                            cAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCCACGt                                       >  1:1081226/1‑100 (MQ=255)
                                                              ggCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTg                                     >  2:2550511/1‑100 (MQ=255)
                                                               gCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGc                                    <  2:2733040/100‑1 (MQ=255)
                                                               gCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGc                                    <  1:181159/100‑1 (MQ=255)
                                                                 gTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACATGCTCCGGCTCGTTTACACTGGCTGGCAACGTTGCTg                                  <  2:457399/100‑1 (MQ=255)
                                                                 gTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTg                                  >  2:2902149/1‑100 (MQ=255)
                                                                   tGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgat                                <  1:2692792/100‑1 (MQ=255)
                                                                    ggCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatn                               <  1:1824805/100‑2 (MQ=255)
                                                                    ggCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatg                               >  2:2026551/1‑100 (MQ=255)
                                                                      cATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatgga                             >  2:2376433/1‑100 (MQ=255)
                                                                      cATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatgga                             >  1:148270/1‑100 (MQ=255)
                                                                       atatAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatggat                            <  2:553077/100‑1 (MQ=255)
                                                                        tataGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatggatg                           >  1:10015/1‑100 (MQ=255)
                                                                         ataGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgc                          <  2:143301/100‑1 (MQ=255)
                                                                          taGCGTGCCATCGGGCGACTGGTATGCGCTGCTGGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgcg                         <  2:252305/100‑1 (MQ=255)
                                                                          taGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgcg                         <  2:600081/100‑1 (MQ=255)
                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  1:1983269/1‑100 (MQ=255)
                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  2:1545654/1‑100 (MQ=255)
                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  2:151862/1‑100 (MQ=255)
                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCAGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  1:182490/1‑100 (MQ=255)
                                                                                gCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaaaa                   >  2:805499/1‑100 (MQ=255)
                                                                                  cATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTCCACTGGCTGGCAACGTTGCTGATGGCTGCGCTAAAACg                 >  2:3159396/1‑100 (MQ=255)
                                                                                   aTCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGc                >  2:2553165/1‑100 (MQ=255)
                                                                                    tCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGcc               >  1:1676599/1‑100 (MQ=255)
                                                                                     cGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCca              <  1:662694/100‑1 (MQ=255)
                                                                                     cGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCca              <  1:28907/100‑1 (MQ=255)
                                                                                      gggCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCcat             >  1:541214/1‑100 (MQ=255)
                                                                                      gggCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCcat             >  1:1442004/1‑100 (MQ=255)
                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  1:1379913/100‑1 (MQ=255)
                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  1:1237036/100‑1 (MQ=255)
                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  2:3537918/100‑1 (MQ=255)
                                                                                            cTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGt       >  1:2465125/1‑100 (MQ=255)
                                                                                            cTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGt       <  1:3634479/100‑1 (MQ=255)
                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  1:2043275/1‑100 (MQ=255)
                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  1:1668411/1‑100 (MQ=255)
                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  2:1131333/1‑100 (MQ=255)
                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     <  2:2825391/100‑1 (MQ=255)
                                                                                                 aTTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCCACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgctgc  >  1:738770/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
CCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGTGCTGC  >  NC_000913/1156353‑1156549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: