BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,298	T→G	S47A (TCG→GCG)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,298	0	T	G	91.0%	398.4 / 23.8	144	S47A (TCG→GCG)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (7/6); new base G (69/62); total (76/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 144 total aligned reads displayed.

GTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA  >  NC_000913/1245202‑1245394

gTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCTATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCgg                                                                                               >  1:3839092/1‑100 (MQ=255)

gTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAACTGCTCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCgg                                                                                               <  2:1462188/100‑1 (MQ=255)

  tAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCa                                                                                             >  1:2833527/1‑100 (MQ=255)

  tAAAAATGGGGCGGATAGTGACGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGAAAAAAAAAGAAAAAATACCCGTGCGGCa                                                                                             <  2:796176/100‑1 (MQ=255)

   aaaaaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCaa                                                                                            >  1:855381/1‑100 (MQ=255)

    aaaaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAAt                                                                                           >  1:3757803/1‑100 (MQ=255)

      aaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGc                                                                                         <  2:710077/100‑1 (MQ=255)

      aaTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGc                                                                                         <  1:3888603/100‑1 (MQ=255)

       aTGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCt                                                                                        >  1:2657905/1‑100 (MQ=255)

          gggCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCAAAAATACCGGTGCGGCAATGCTTCa                                                                                     >  2:3346171/1‑100 (MQ=255)

           ggCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATCGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAg                                                                                    <  2:1114482/100‑1 (MQ=255)

           ggCGGATAGTGAGGCGGTTATGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAGTACCCGTGCGGCACTGCTTCAg                                                                                    >  2:1249939/1‑100 (MQ=255)

              gatctgTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTa                                                                                 <  2:3965754/95‑1 (MQ=255)

                aTAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTAcg                                                                               <  1:2633884/100‑1 (MQ=255)

                 tAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTAcgc                                                                              >  1:1091756/1‑100 (MQ=255)

                   gTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTaagatc                                                                            >  1:4049234/1‑95 (MQ=255)

                    tGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGaa                                                                           >  1:1022502/1‑100 (MQ=255)

                     gAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAag                                                                          >  2:1240225/1‑100 (MQ=255)

                      aGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAaga                                                                         <  1:1622097/100‑1 (MQ=255)

                      aGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGAGCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAaga                                                                         >  2:3075774/1‑100 (MQ=255)

                        gCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAgg                                                                       <  2:1574084/100‑1 (MQ=255)

                         cGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGa                                                                      <  2:3879014/100‑1 (MQ=255)

                         cGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGa                                                                      >  2:1799856/1‑100 (MQ=255)

                          ggTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGaa                                                                     >  1:3895088/1‑100 (MQ=255)

                           gTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAAt                                                                    >  2:2369495/1‑100 (MQ=255)

                            ttttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATg                                                                   >  1:1630706/1‑100 (MQ=255)

                             tttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATgg                                                                  <  2:2739579/100‑1 (MQ=255)

                              ttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCC‑AAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTc                                                                <  2:3523203/100‑1 (MQ=255)

                                ggCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCt                                                               >  2:2374396/1‑100 (MQ=255)

                                ggCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCt                                                               >  1:2192515/1‑100 (MQ=255)

                                 gCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTg                                                              >  1:1364568/1‑100 (MQ=255)

                                 gCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTg                                                              <  1:2408185/100‑1 (MQ=255)

                                  cTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGa                                                             >  2:2769100/1‑100 (MQ=255)

                                   tGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                            >  2:539138/1‑100 (MQ=255)

                                   tGCGCTTATTGAACAGTTAGAATTGATCAATCCTTCTGGTAGATTAAGCCAAAATACACGTTCGGTAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                            <  2:2615580/100‑1 (MQ=255)

                                    gcgcTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAt                                                           <  1:2464383/100‑1 (MQ=255)

                                    gcgcTTATTGAACAGGTAAAATTGATCAATCATTCTGGCATATTAAGGCAAAATACCCTTGCGGCAATGCTTCAGTTACGCGAAGAGGAATTGTCTGAAt                                                           <  2:985517/100‑1 (MQ=255)

                                     cgcTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtt                                                          >  1:2941209/1‑100 (MQ=255)

                                     cgcTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtt                                                          >  1:3723238/1‑100 (MQ=255)

                                         tATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttt                                                      >  1:1303148/1‑100 (MQ=255)

                                         tATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttt                                                      >  1:2713462/1‑100 (MQ=255)

                                           ttGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttttt                                                    >  1:2653411/1‑100 (MQ=255)

                                            tGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAATCCACAATACACGTGCGGCAATGCTTAAGTTACGCGAAGAGGAATGGTCTGAATTCttttta                                                   >  2:4002423/1‑99 (MQ=255)

                                            tGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttttt                                                   >  1:2042051/1‑100 (MQ=255)

                                            tGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttttt                                                   >  2:1665259/1‑100 (MQ=255)

                                             gAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCCATGCTTCAGTTACGCGAAGAGGCATGGTCTGAATTCttttttt                                                  >  2:1698016/1‑100 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTGAGCCACAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                                 <  2:3820091/100‑1 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttttttt                                                 >  2:2082644/1‑99 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                                 >  1:1795787/1‑100 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                                 <  2:3644101/100‑1 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                                 <  2:3961923/100‑1 (MQ=255)

                                              aaCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                                 <  1:4055991/100‑1 (MQ=255)

                                               aCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAGTTCTTTTTTTgg                                                <  1:3510111/100‑1 (MQ=255)

                                               aCATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTgg                                                >  1:3280804/1‑100 (MQ=255)

                                                cATTTAGAATTGATCAATCCTTCTGGCAGATTAAGTCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGt                                               <  2:242067/100‑1 (MQ=255)

                                                 aTTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt                                              >  1:606907/1‑100 (MQ=255)

                                                 aTTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt                                              <  1:710935/100‑1 (MQ=255)

                                                   ttAGAATTGATCAATCCTTCTGGCAGATTAAGCAAAAATACGCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGc                                            <  2:543818/100‑1 (MQ=255)

                                                     aGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTa                                          >  1:1642806/1‑100 (MQ=255)

                                                      gAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTaa                                         >  2:815281/1‑100 (MQ=255)

                                                        aTTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAAt                                       <  1:224852/100‑1 (MQ=255)

                                                        aTTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAAt                                       <  2:3338862/100‑1 (MQ=255)

                                                          tGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtc                                     <  2:2663984/100‑1 (MQ=255)

                                                          tGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtc                                     <  1:3392807/100‑1 (MQ=255)

                                                           gATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtct                                    >  2:1249226/1‑100 (MQ=255)

                                                           gATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtct                                    >  1:1006159/1‑100 (MQ=255)

                                                            aTCAATCCTTCTGGCAGATTAAGCCAAAATAACCGTGCGCCAATGCTTCCGTTACGCGAAACGGCAAGATCCGAATTCTTTTTTTGGTTTCCAAACtcct                                   >  2:3157053/1‑98 (MQ=255)

                                                              cAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTg                                 <  2:740047/100‑1 (MQ=255)

                                                              cAATCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTg                                 <  2:594502/100‑1 (MQ=255)

                                                               aaTCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTgg                                >  1:853754/1‑100 (MQ=255)

                                                               aaTCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTgg                                <  1:2034958/100‑1 (MQ=255)

                                                                aTCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGa                               >  1:2295698/1‑100 (MQ=255)

                                                                 tCCTTCTGGGAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                              <  2:3505330/100‑1 (MQ=255)

                                                                 tCCTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                              <  2:2064294/100‑1 (MQ=255)

                                                                 gccTTCTGGCAGATTAGGCCAAAATACCCGTGCGGCAATGGTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                              <  2:3238032/99‑1 (MQ=255)

                                                                  ccTTCTGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgt                             >  2:861120/1‑100 (MQ=255)

                                                                       tGGCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTg                        <  2:3989158/100‑1 (MQ=255)

                                                                        ggCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTgg                       >  1:2659761/1‑100 (MQ=255)

                                                                         gCAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGa                      <  2:1050809/100‑1 (MQ=255)

                                                                          cAGATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGAt                     >  2:2461470/1‑100 (MQ=255)

                                                                            gATTAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGACTGAATTCTTTTTTTGATTGCTAAATTCTCTGGAGTGTCTGGATTa                   >  2:1381452/1‑100 (MQ=255)

                                                                              ttAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtg                 <  1:977814/100‑1 (MQ=255)

                                                                               tAAGCCGAGATCCCCGTGCGGCAATGCTTTAGTTACGCGAAGAGGAATGGTGTGAATTCTGTTTTTGGTTGCTGAATTCTCCGGAGTGTCTGGATTAtgt                <  2:2897183/100‑1 (MQ=255)

                                                                               tAAGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgt                <  1:3014762/100‑1 (MQ=255)

                                                                                aaGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGACGAGGAATGGTCTGAATTTTTTTTTTGGTTGCTAAATCCTCTGGAGTGTCTAGATTAtgag               >  1:3555348/1‑98 (MQ=255)

                                                                                aaGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg               <  1:333621/100‑1 (MQ=255)

                                                                                 aGCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTTGTTTCTAAATTCTCTGGAGTGTCTGGATTATGTGa              >  2:1928972/1‑100 (MQ=255)

                                                                                  gCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat             >  2:2124792/1‑100 (MQ=255)

                                                                                  gCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGTTTTCTAAATTCTCTGGAGTGGCTGGATTATGTGat             >  2:2199873/1‑100 (MQ=255)

                                                                                  gCCAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat             >  1:362335/1‑100 (MQ=255)

                                                                                   ccAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGGTTATGTGatt            >  2:1070806/1‑100 (MQ=255)

                                                                                   ccAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt            >  2:2707959/1‑100 (MQ=255)

                                                                                   ccAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt            >  1:522144/1‑100 (MQ=255)

                                                                                    cAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatta           >  1:107867/1‑100 (MQ=255)

                                                                                    cAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatta           >  2:3705189/1‑100 (MQ=255)

                                                                                    cAAAATACCCGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTCGTTGCCAAATTCTCTGGAGTGTCTGGATTATGTGatta           <  1:3626866/100‑1 (MQ=255)

                                                                                        aTACCCGTGCGGCAATGCTTCAGTGACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTaa       <  1:1072302/100‑1 (MQ=255)

                                                                                           cccGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTAGTAAttt    >  2:3530849/1‑100 (MQ=255)

                                                                                            ccGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTa   <  1:1574588/100‑1 (MQ=255)

                                                                                             cGTGCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTaa  >  2:2818324/1‑100 (MQ=255)

GTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA  >  NC_000913/1245202‑1245394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`