Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,290,933 T→G L231R (CTT→CGT)  rssB → PcnB‑degradosome interaction factor; response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,290,9330TG98.7% 524.0 / NA 152L231R (CTT→CGT) rssBPcnB‑degradosome interaction factor; response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (84/64);  total (85/67)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCT  >  NC_000913/1290836‑1291029
                                                                                                 |                                                                                                
gCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTg                                                                                                >  1:1997799/1‑100 (MQ=255)
 cATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGc                                                                                               <  1:3174008/100‑1 (MQ=255)
 cATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGc                                                                                               <  1:525118/100‑1 (MQ=255)
  aTTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGca                                                                                              >  2:1393613/1‑100 (MQ=255)
  aTTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGca                                                                                              >  2:3960552/1‑100 (MQ=255)
   ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGcac                                                                                             <  1:3025036/100‑1 (MQ=255)
   gttgtttGCGTTGATTGCACCCTTTCTTGACATAATGTCGTACTGGCTTTCTTGGTATTGCGCGCTTTGGTTAACGGATTATTACAGGAACAGCTTGcac                                                                                             <  2:3019882/95‑1 (MQ=255)
       aTTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCa                                                                                         <  1:2789818/100‑1 (MQ=255)
         tGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaa                                                                                       <  1:999321/100‑1 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      >  1:792139/1‑100 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      >  2:2668588/1‑100 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      <  2:286433/100‑1 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      >  1:895764/1‑100 (MQ=255)
          gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCaaaa                                                                                      <  2:700715/100‑1 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     >  1:3965017/1‑100 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     <  2:741971/100‑1 (MQ=255)
           ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAAt                                                                                     <  1:300032/100‑1 (MQ=255)
            cTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATc                                                                                    <  1:252057/100‑1 (MQ=255)
            cTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATc                                                                                    <  2:264129/100‑1 (MQ=255)
              tGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCaa                                                                                  <  2:1152041/100‑1 (MQ=255)
                 tGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACgg                                                                               >  2:2978430/1‑100 (MQ=255)
                  gTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGt                                                                              >  1:1032209/1‑100 (MQ=255)
                   tCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGtt                                                                             <  1:3224664/100‑1 (MQ=255)
                    cACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTg                                                                            >  2:1939995/1‑100 (MQ=255)
                    cACCCGAACTGGACATAATAGCGTACTTGGTGCATTGTTATTACACGCATTGTTTAACGGATTCATCCAGGAACAGAGTGCACACCAAAATAAACCGtta                                                                            >  2:3108533/1‑99 (MQ=255)
                       ccGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCa                                                                         >  2:2523406/1‑100 (MQ=255)
                           gCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGt                                                                     >  1:2898116/1‑100 (MQ=255)
                             tGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTg                                                                   <  1:2358105/100‑1 (MQ=255)
                             tGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCATGCACACCAAAATCAACGGTTGCCAGAGTTg                                                                   >  2:3135628/1‑100 (MQ=255)
                              ggACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTgg                                                                  >  1:831988/1‑100 (MQ=255)
                                 cATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGgcg                                                               >  1:2456847/1‑100 (MQ=255)
                                  aTAATGGCGTACTTGCTGCCTTGTTATTACGCGCAGTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAACTCAACGGTTGCCAGAGTTGGgcgc                                                              <  2:3268341/100‑1 (MQ=255)
                                   tAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGcgcg                                                             >  2:1655331/1‑100 (MQ=255)
                                    aaTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATGATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCTCGt                                                            >  2:815828/1‑100 (MQ=255)
                                     aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           <  2:521499/100‑1 (MQ=255)
                                     aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                                           >  1:3897296/1‑100 (MQ=255)
                                       ggCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAt                                                         >  2:1884882/1‑100 (MQ=255)
                                         cGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACGAAAATCAACGGTTGCCATAGTTGGGCGCGTTCTTg                                                       >  2:3376125/1‑100 (MQ=255)
                                         cGTACTTGCTGCCTTGTTAGTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGGTGGGCGCGTTATTg                                                       >  2:3654324/1‑100 (MQ=255)
                                          gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                      <  1:286300/100‑1 (MQ=255)
                                          gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                      >  1:1585932/1‑100 (MQ=255)
                                          gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGa                                                      <  1:2822211/100‑1 (MQ=255)
                                           tACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGaa                                                     >  2:1220886/1‑100 (MQ=255)
                                           tACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGaa                                                     >  2:2055363/1‑100 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAtagatc                                                    >  1:879846/1‑95 (MQ=255)
                                            aCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                                                    <  2:628377/100‑1 (MQ=255)
                                              ttGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGcn                                                  <  1:3234318/100‑2 (MQ=255)
                                              ttGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCa                                                  <  1:2362919/100‑1 (MQ=255)
                                              ttGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCa                                                  <  2:1844747/100‑1 (MQ=255)
                                               tgctgcCTTGTTATTACGCGCATTGTTTAACGGATGATTACAGGAACAGCGTGCACACCAAAATCAACGGTTCCCAGAGTTGGGCGCGTTATTGAAGCAg                                                 >  2:2191264/1‑100 (MQ=255)
                                                tctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAgg                                                <  2:1491702/99‑1 (MQ=255)
                                                   gcCTTGTTATTCCTCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCTTACCAAAATCAACGGTTGCCAGCGTTGGGCGCGTTATTGAAGCAGGTaa                                             >  2:203876/1‑100 (MQ=255)
                                                   gcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTTCCAGAGTTGGGCGCGTTATTGAAGCAGGTaa                                             <  2:3613120/100‑1 (MQ=255)
                                                    ccTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTaaa                                            >  2:650149/1‑100 (MQ=255)
                                                     cTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                                           >  2:3263759/1‑100 (MQ=255)
                                                     cTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                                           >  1:1505654/1‑100 (MQ=255)
                                                     cTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACAGTTGCCAGAGTGGGGAGCGTTATTGAAGCAGGTAAAc                                           >  1:3798698/1‑100 (MQ=255)
                                                       tGTTATTACGCGCATTGTTTAACTTATTATTACAGGAACAGCGTGCACACCAATGTCAACGTTTGCCATAGTTGGGCGCGTTATTGAAGCAGTTAAACCa                                         <  2:2401157/100‑1 (MQ=255)
                                                       tGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCa                                         >  1:3447199/1‑100 (MQ=255)
                                                       tGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCa                                         <  2:1729315/100‑1 (MQ=255)
                                                         ttattaCGCGCATTGTTGAACGGATTATTACAGGAGCAGCGTGCACACCGGAATCAACGGTTGCGAGAGGTGGGCGCGTTATTGAAGCAGGTAAACCAtt                                       <  2:2521798/100‑1 (MQ=255)
                                                          tattaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCAtta                                      >  1:2508592/1‑99 (MQ=255)
                                                           attaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTa                                     <  2:876899/100‑1 (MQ=255)
                                                           attaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTa                                     >  1:3637103/1‑100 (MQ=255)
                                                            ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTAc                                    <  2:1735510/100‑1 (MQ=255)
                                                             taCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACt                                   >  1:48579/1‑100 (MQ=255)
                                                              aCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACtt                                  <  2:494681/100‑1 (MQ=255)
                                                              aCGCGCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACtt                                  <  1:3492030/100‑1 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACttt                                 >  2:2940873/1‑99 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTGTTGGAGCAGGTAAACCATTTACTTc                                 >  2:3787619/1‑100 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                 >  1:3898409/1‑100 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                 >  1:2442771/1‑100 (MQ=255)
                                                               cgcgCATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                 >  1:1836974/1‑100 (MQ=255)
                                                                gcgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGTGCGTTATTGAAGCAGGTAAACCATTTACTTCg                                <  1:3160898/100‑1 (MQ=255)
                                                                gcgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg                                <  2:1780985/100‑1 (MQ=255)
                                                                gcgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg                                <  1:577593/100‑1 (MQ=255)
                                                                 cgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGt                               >  2:1803270/1‑100 (MQ=255)
                                                                 cgcATTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGt                               >  1:1497090/1‑100 (MQ=255)
                                                                    aTTGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAg                            >  2:1173336/1‑100 (MQ=255)
                                                                    aTTGTTTAACGGATTATTACAGGAACAGCGTACACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAg                            >  1:1790788/1‑100 (MQ=255)
                                                                     ttGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAgg                           >  2:1829769/1‑100 (MQ=255)
                                                                      tGTTTAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGc                          <  1:1047565/100‑1 (MQ=255)
                                                                        tttAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTACACCATTTACTTCGTCAGGCCa                        >  2:3424694/1‑100 (MQ=255)
                                                                         ttAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCaa                       >  1:1632472/1‑100 (MQ=255)
                                                                          tAACGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAAt                      <  1:1797439/100‑1 (MQ=255)
                                                                             cGGATTATTACAGGAACCGCGTGCACACCAAAATCAACGGTTGCCAGCGCTGGGCGCGTTATTGAAGCAGGTAGACCATTCCCTTCGTCAGGCCAATATg                   >  2:834968/1‑100 (MQ=255)
                                                                             cGGATTATTACAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTg                   >  1:942440/1‑100 (MQ=255)
                                                                                 ttattaCAGGAACAGCGTGCACACCAAAATCAGCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGccag               <  2:1605139/100‑3 (MQ=255)
                                                                                  tattaCAGGAACAGCGTGCACACCAAGATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCggg              <  2:2447991/100‑1 (MQ=255)
                                                                                    ttaCAGGAACAGCGTGCACACCGAAATCTACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGc            <  1:3710843/100‑1 (MQ=255)
                                                                                    ttaCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGc            <  2:2257948/100‑1 (MQ=255)
                                                                                    ttaCAGGAACAGCGTGCACACCAAAAGCAACGGTTGCCAGAGTTGCGCGCGTTATTGAAGCAGGTAAACCCTTTAGTTCGTCAGGCCAATCTGCCGGGGc            <  1:3811425/100‑1 (MQ=255)
                                                                                    ttaCAGGAACAGCCTGCACACCAAAATCAACTGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGc            <  2:3134116/100‑1 (MQ=255)
                                                                                     taCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCa           >  1:3091415/1‑100 (MQ=255)
                                                                                     taCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCa           >  1:2115662/1‑100 (MQ=255)
                                                                                      aCAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAg          <  1:3964996/100‑1 (MQ=255)
                                                                                       cAGGAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGTGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGt         >  2:3863531/1‑100 (MQ=255)
                                                                                          gAACAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTc      >  1:605621/1‑100 (MQ=255)
                                                                                           aaCAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTcc     <  1:2222929/100‑1 (MQ=255)
                                                                                           aaCAGCGTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTcc     <  2:3613401/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCT  >  NC_000913/1290836‑1291029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: