Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,311,596 T→G L170V (TTG→GTG)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,5960TG98.2% 551.4 / 0.3 162L170V (TTG→GTG) tonBmembrane spanning protein in TonB‑ExbB‑ExbD transport complex
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (92/67);  total (94/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 162 total aligned reads displayed.

AGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATG  >  NC_000913/1311499‑1311691
                                                                                                 |                                                                                               
aGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTg                                                                                               >  1:831996/1‑100 (MQ=255)
 gCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTgc                                                                                              >  1:2675280/1‑100 (MQ=255)
 gCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTgc                                                                                              >  1:1285428/1‑100 (MQ=255)
   aaCGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTgcgc                                                                                            >  1:2084546/1‑100 (MQ=255)
       gCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTg                                                                                        >  2:440893/1‑100 (MQ=255)
       gCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTg                                                                                        >  2:3637311/1‑100 (MQ=255)
        cTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGa                                                                                       >  1:1730024/1‑100 (MQ=255)
        cTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGa                                                                                       >  2:669486/1‑100 (MQ=255)
          gCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAg                                                                                     >  1:764224/1‑100 (MQ=255)
            aaCCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAggg                                                                                   >  1:3629483/1‑100 (MQ=255)
              ccAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCa                                                                                 <  2:3187744/100‑1 (MQ=255)
              ccAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCa                                                                                 >  1:132599/1‑100 (MQ=255)
               cAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAg                                                                                <  1:3541625/100‑1 (MQ=255)
                aGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAgg                                                                               >  1:856885/1‑100 (MQ=255)
                aGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAgg                                                                               >  1:2199494/1‑100 (MQ=255)
                 gCAAGCCGGTTACCAGCGTGGCATCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTACCCGGCACGAGCACAGGCAGCGCGCATTGAAGGGCAGgt                                                                              <  2:421089/100‑1 (MQ=255)
                    aGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaa                                                                           >  2:1086715/1‑100 (MQ=255)
                     gCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaa                                                                          >  2:3602873/1‑100 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         >  1:930774/1‑100 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         <  1:1023052/100‑1 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         <  1:553688/100‑1 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         <  1:2882368/100‑1 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         >  1:1182045/1‑100 (MQ=255)
                      ccGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaag                                                                         >  1:1342538/1‑100 (MQ=255)
                       ctgTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGGAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaagt                                                                        <  2:109814/98‑1 (MQ=255)
                           tACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgttaaagttaaa                                                                    <  1:2192205/100‑1 (MQ=255)
                           tACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGgataaagttaaa                                                                    >  2:3029988/1‑100 (MQ=255)
                            aCCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAAt                                                                   <  1:3709994/100‑1 (MQ=255)
                              cAGTGTGGCTGCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAAttt                                                                 <  1:2332320/100‑1 (MQ=255)
                               atcttgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTg                                                                <  2:3784472/96‑1 (MQ=255)
                                 tgtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGAc                                                              <  2:2693096/100‑1 (MQ=255)
                                 tgtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGAc                                                              <  2:2845479/100‑1 (MQ=255)
                                  gtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACg                                                             <  1:1600448/100‑1 (MQ=255)
                                  gtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACg                                                             <  1:3727956/100‑1 (MQ=255)
                                  gtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACg                                                             <  1:3371530/100‑1 (MQ=255)
                                  gtgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACg                                                             <  2:197568/100‑1 (MQ=255)
                                   tgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGagat                                                            >  1:3865600/1‑97 (MQ=255)
                                   tgGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGt                                                            >  2:2708046/1‑100 (MQ=255)
                                     gCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTa                                                          >  1:886339/1‑100 (MQ=255)
                                      cTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTAc                                                         <  2:3703065/100‑1 (MQ=255)
                                      cTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTAc                                                         >  1:1504292/1‑100 (MQ=255)
                                      cTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTAc                                                         >  1:803008/1‑100 (MQ=255)
                                      cTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTGAAAGTTAAATTTGACGTTAc                                                         >  1:1782672/1‑100 (MQ=255)
                                         cAGGACCACGCGCATTGAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGcc                                                      <  1:3356117/100‑1 (MQ=255)
                                          aGGACCACGCGCCTTAAGCCGTAATCAACCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGGTAAAGTTAAATTTGACGTTACGCCg                                                     >  1:3601081/1‑100 (MQ=255)
                                          aGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTccgccg                                                     <  2:2377588/100‑1 (MQ=255)
                                           ggACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGccag                                                    >  1:3200831/1‑98 (MQ=255)
                                             aCCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGAt                                                  <  2:1772656/100‑1 (MQ=255)
                                             aCCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGAt                                                  >  2:2966848/1‑100 (MQ=255)
                                             aCCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGAt                                                  >  1:3690084/1‑100 (MQ=255)
                                              ccACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATg                                                 <  2:2335171/100‑1 (MQ=255)
                                               cACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATgg                                                >  2:825510/1‑100 (MQ=255)
                                                aCGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGt                                               >  2:3489757/1‑100 (MQ=255)
                                                 cgcgCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATCGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTc                                              >  2:2535653/1‑100 (MQ=255)
                                                  gcgcATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTcg                                             <  2:3044112/100‑1 (MQ=255)
                                                  gcgcATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTcg                                             <  1:101196/100‑1 (MQ=255)
                                                    gcATTAAGCTGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTcgcg                                           <  2:3458815/100‑1 (MQ=255)
                                                    gcATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTcgcg                                           <  1:2592752/100‑1 (MQ=255)
                                                     cATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGt                                          <  2:1487486/100‑1 (MQ=255)
                                                     cATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGt                                          >  2:2090841/1‑100 (MQ=255)
                                                      aTTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTg                                         >  1:1647696/1‑100 (MQ=255)
                                                      aTTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTg                                         <  1:2098263/100‑1 (MQ=255)
                                                        tAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGa                                       <  1:3566822/100‑1 (MQ=255)
                                                         aaGCCGTAATCAGCCGCAGTATCCGGTAAGAGTACAGGCAGTGCGCATTGAGGGGCAGGTAAAATTTAACTTTGCCGTTACGCCGGATGGTCGCGTGGAt                                      <  2:131190/100‑1 (MQ=255)
                                                          aGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATa                                     <  2:12024/100‑1 (MQ=255)
                                                           gCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATaa                                    >  2:741827/1‑100 (MQ=255)
                                                            ccGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAc                                   <  1:726025/100‑1 (MQ=255)
                                                            ccGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAc                                   >  2:2982508/1‑100 (MQ=255)
                                                            ccGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAc                                   <  2:3193369/100‑1 (MQ=255)
                                                            ccGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAc                                   >  2:3681480/1‑100 (MQ=255)
                                                             cGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTTACGTTACGCCGGATGGTCGCGTGGATAACg                                  >  2:1605829/1‑100 (MQ=255)
                                                             cGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACg                                  >  2:2199757/1‑100 (MQ=255)
                                                                 aTCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACa                              <  2:1889634/100‑1 (MQ=255)
                                                                 aTCAGCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACa                              <  2:3123571/100‑1 (MQ=255)
                                                                     gCCGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATc                          >  2:2387511/1‑100 (MQ=255)
                                                                      ccGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATcc                         >  2:721920/1‑100 (MQ=255)
                                                                      ccGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATcc                         <  1:2464576/100‑1 (MQ=255)
                                                                       cGCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCct                        >  1:4067782/1‑100 (MQ=255)
                                                                        gCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCctc                       >  2:1428551/1‑100 (MQ=255)
                                                                        gCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCctc                       <  2:2034047/100‑1 (MQ=255)
                                                                        gCAGTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCctc                       >  1:1903053/1‑100 (MQ=255)
                                                                           gTATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCa                    >  1:1166665/1‑100 (MQ=255)
                                                                            tATCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAg                   <  1:3171294/100‑1 (MQ=255)
                                                                             aTCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGc                  >  2:3541052/1‑100 (MQ=255)
                                                                             aTCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGc                  <  2:18911/100‑1 (MQ=255)
                                                                             aTCCGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGc                  <  1:961220/100‑1 (MQ=255)
                                                                               ccGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCa                >  1:2808103/1‑100 (MQ=255)
                                                                               ccGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCa                <  1:449427/100‑1 (MQ=255)
                                                                                cGGCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGATACGCAGGCTGGTCGCGGAGATAAGGTACAAATCCTCTCAGCCaa               >  1:3816138/1‑100 (MQ=255)
                                                                                 ggCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAg              >  1:4015621/1‑100 (MQ=255)
                                                                                 ggCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAg              <  1:1840271/100‑1 (MQ=255)
                                                                                 ggCACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAg              <  2:1730259/100‑1 (MQ=255)
                                                                                   cACGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTGAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGcc            <  2:2294275/100‑1 (MQ=255)
                                                                                     cGAGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTg          <  1:1943636/100‑1 (MQ=255)
                                                                                       aGCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCg        >  2:1112091/1‑100 (MQ=255)
                                                                                        gCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGa       >  1:3818001/1‑100 (MQ=255)
                                                                                        gCACAGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGa       >  1:2772233/1‑100 (MQ=255)
                                                                                          acaGGCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAAc     <  1:2002536/100‑1 (MQ=255)
                                                                                             ggCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATg  >  1:1448403/1‑100 (MQ=255)
                                                                                             ggCAGTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATg  <  2:1200193/100‑1 (MQ=255)
                                                                                                 |                                                                                               
AGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAACGTACAAATCCTCTCAGCCAAGCCTGCGAACATG  >  NC_000913/1311499‑1311691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑