Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 95,418 | T→G | S257A (TCG→GCG) | murF → | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 95,418 | 0 | T | G | 99.3% | 482.1 / ‑4.7 | 138 | S257A (TCG→GCG) | murF | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (70/67); total (71/67) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 |
Only 100 of 143 total aligned reads displayed.
GGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAG > NC_000913/95320‑95514 | ggCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTc > 2:3428732/1‑100 (MQ=255) gCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACcgcg < 2:1450302/100‑1 (MQ=255) cTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCgcgc < 2:3174536/100‑1 (MQ=255) tGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa < 1:3409415/100‑1 (MQ=255) tGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCa < 1:3316385/100‑1 (MQ=255) aaCTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACg < 1:3432356/100‑1 (MQ=255) aCTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACgg > 1:1324096/1‑100 (MQ=255) aCTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATCCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACgg > 2:2436591/1‑100 (MQ=255) cTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGt > 1:1087366/1‑100 (MQ=255) cTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGt < 1:1362175/100‑1 (MQ=255) ggCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTAc > 2:2713130/1‑100 (MQ=255) ggCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTAc > 2:1061557/1‑100 (MQ=255) ggCAGAGCGTAATTGGCTCACGCAAAGTGTGACGTTTCTCGCCCAAGGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTAc > 2:2565725/1‑100 (MQ=255) gCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACg > 2:3520701/1‑100 (MQ=255) agagCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGa > 2:3757404/1‑100 (MQ=255) agagCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGa > 1:3995466/1‑100 (MQ=255) agCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAAt < 1:624629/100‑1 (MQ=255) gTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTa < 2:2609480/100‑1 (MQ=255) aaTTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTAcc < 1:2104708/100‑1 (MQ=255) aaTTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTAcc < 1:3507899/100‑1 (MQ=255) aTTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTAccc < 1:1027627/100‑1 (MQ=255) ttGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCt < 2:2569846/100‑1 (MQ=255) ttGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCt < 2:698543/100‑1 (MQ=255) aCGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAcc < 1:931830/100‑1 (MQ=255) aCGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAcc < 1:1186374/100‑1 (MQ=255) aCGCAAAGTGTGGCGTTTCTCACCAAATGGCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAcc < 2:397657/100‑1 (MQ=255) gCAAAGTGTGCCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAAcccc < 2:3960564/100‑1 (MQ=255) cAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCa < 2:1625186/100‑1 (MQ=255) cAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCa > 2:299534/1‑100 (MQ=255) aGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAAcc < 1:3994912/100‑1 (MQ=255) gtgtGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCg < 2:2263049/100‑1 (MQ=255) gtgGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGt > 2:749419/1‑100 (MQ=255) tgGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTa < 2:3928048/100‑1 (MQ=255) ggCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAg < 1:3103463/100‑1 (MQ=255) gCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGc > 1:1648796/1‑100 (MQ=255) cGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCg > 2:2706008/1‑100 (MQ=255) ctcACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGAt < 2:64135/100‑1 (MQ=255) ctcAACCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCCACGGGTAGCGTCGAt < 2:1033778/100‑1 (MQ=255) tcACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATg > 1:841604/1‑100 (MQ=255) tcACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATg < 1:3369265/100‑1 (MQ=255) cACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAGCCGGTAGCGTCGATGt < 1:407517/100‑1 (MQ=255) aCCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCCTGTCACCGCGCACGGTACGGACTTTACCCTACAAACCCCAACCGGTAGCGACCATGtt > 2:531398/1‑100 (MQ=255) aCCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGtt > 1:3489627/1‑100 (MQ=255) cccAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTc < 1:963124/100‑1 (MQ=255) cccAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTc > 2:1925504/1‑100 (MQ=255) ccACTGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTct < 1:2299185/100‑1 (MQ=255) cAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTctg > 1:3793788/1‑100 (MQ=255) aaTGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTctgc < 1:171810/100‑1 (MQ=255) aaTGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTctgc < 1:1995099/100‑1 (MQ=255) gccgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCtgctgc > 1:759337/1‑100 (MQ=255) ccgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGcc > 1:1827838/1‑100 (MQ=255) cgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCg > 1:563203/1‑100 (MQ=255) cgccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCg > 1:218937/1‑100 (MQ=255) gccAACAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGt < 1:1952916/100‑1 (MQ=255) aCAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGcc < 1:2206140/100‑1 (MQ=255) cAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCg > 2:1592190/1‑100 (MQ=255) cAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCg > 2:3017196/1‑100 (MQ=255) cAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCg > 2:254534/1‑100 (MQ=255) cAGCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCg > 2:2266836/1‑100 (MQ=255) gCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCggg > 1:3188781/1‑100 (MQ=255) gCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCggg < 2:562728/100‑1 (MQ=255) gCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCggg > 2:2608253/1‑100 (MQ=255) gCGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCggg > 1:3642786/1‑100 (MQ=255) cGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAGCCCCAGCCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg < 2:3140171/100‑1 (MQ=255) cGATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCgggg > 1:2212003/1‑100 (MQ=255) gATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGc > 1:3781228/1‑100 (MQ=255) gATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGc > 2:2469936/1‑100 (MQ=255) gATTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGc > 2:1517986/1‑100 (MQ=255) aTTTCACCGCCACCAATATCCATGTGAGCGCGCACCTTACGCAATAAACCCTACAAACCCCAACCGGCAGCGTCGATGTTCTGCGGCCCTTGCCGGGGCg > 2:2184132/1‑100 (MQ=255) aTTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAGCCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg < 2:1050425/100‑1 (MQ=255) aTTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCACCTGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg < 2:3905336/100‑1 (MQ=255) aTTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg < 2:1284267/100‑1 (MQ=255) aTTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg < 2:3174624/100‑1 (MQ=255) aTTTCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCg < 2:3042175/100‑1 (MQ=255) tttCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGt > 1:2420977/1‑100 (MQ=255) tttCACCGCCACCAATAGCCATGTGACCGCGCACGGTACGGAATTTACCCTAGAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGt < 2:2234194/100‑1 (MQ=255) ttCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTc > 2:2018695/1‑100 (MQ=255) ttCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTc < 2:2889074/100‑1 (MQ=255) ttCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTc > 2:2019612/1‑100 (MQ=255) tCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTca > 1:3547181/1‑100 (MQ=255) tCACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTca > 2:2165007/1‑100 (MQ=255) cACGGCCACCAATATCCATGTGACCGCGCACGGTACGGCATTTACCCTACAAGTTCCAACCGGCAGAGTCGATGTTCTGATGCCGTTGCCGGGGCGCcac > 2:441100/1‑100 (MQ=255) cACCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCCGCCGTTGCCGGGGCGTcac > 1:3364606/1‑100 (MQ=255) aCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTcaca > 2:2081533/1‑100 (MQ=255) aCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTcaca < 2:1621252/100‑1 (MQ=255) aCCGCCACCAATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGCGGCGCcaca > 2:2585265/1‑100 (MQ=255) aCCGCCACCAATATCCATGTCACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTCCTGCCGTTGCCGGGGCGTcaca > 2:2181716/1‑100 (MQ=255) agcaccaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAata < 2:3602975/98‑1 (MQ=255) caccaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATAtt > 2:3773098/1‑100 (MQ=255) ccaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGc > 2:1806878/1‑100 (MQ=255) ccaATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGc < 2:1798349/100‑1 (MQ=255) caATATCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCg < 2:1339968/100‑1 (MQ=255) natatCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGa > 1:3231957/2‑100 (MQ=255) atCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATg < 1:2330547/100‑1 (MQ=255) tCCATGTGACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATgc > 1:2353692/1‑100 (MQ=255) ccATGTGACCGCGCACGGTACGGAATTTACCCTACAAACTCCAACCGGTAGCGTCCATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGAGAATgcg > 1:3748345/1‑100 (MQ=255) tgACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGc > 1:2694291/1‑100 (MQ=255) gACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCa < 2:637764/100‑1 (MQ=255) gACCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCa > 1:2395916/1‑100 (MQ=255) aCCGCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAGTGCGCTCGCAg > 2:2532191/1‑100 (MQ=255) | GGCTGAACTGGCAGAGCGTAATTGGCTCACGCAAAGTGTGGCGTTTCTCACCCAATGCCGCCAACAGCGATTTCACCGCCACCAATATCCATGTGACCTCGCACGGTACGGAATTTACCCTACAAACCCCAACCGGTAGCGTCGATGTTCTGCTGCCGTTGCCGGGGCGTCACAATATTGCGAATGCGCTGGCAG > NC_000913/95320‑95514 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |