BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,936	A→C	N267H (AAC→CAC)	ynbC →	putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,936	0	A	C	99.2%	452.5 / NA	133	N267H (AAC→CAC)	ynbC	putative esterase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (72/59); total (72/61)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACA  >  NC_000913/1479839‑1480031

cctctaaCCGCCGACTGATTTTTATCCGAGATTACCTCGTCCGCTGTAAGCGCTGCATCGGCTGCCAGGCTTTTATCACGACACGTTAGGTAAACAGCAc                                                                                               <  2:58858/95‑1 (MQ=255)

 cGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGcaca                                                                                              >  2:3776613/1‑100 (MQ=255)

  gCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAg                                                                                             <  2:2051165/100‑1 (MQ=255)

   ccAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCATTTATCACGACACGTTGGGTGAAGAGCACAgg                                                                                            >  1:3739187/1‑100 (MQ=255)

    cAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGTTTTTATCACGACACGTTGGGTGAAGAGCACAggg                                                                                           <  2:1522803/100‑1 (MQ=255)

    cAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAggg                                                                                           <  2:1456882/100‑1 (MQ=255)

     aaCCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgc                                                                                          <  1:895379/100‑1 (MQ=255)

      aCCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcg                                                                                         >  2:1235887/1‑100 (MQ=255)

       ccGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcgc                                                                                        >  1:2986051/1‑100 (MQ=255)

        cGCAGATTGATTTTTATCAGAGGTTACGGAGTCCTCTGAAAGAGCTGGGTCTGCGGCCAGGCGTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCt                                                                                       <  2:1353535/100‑1 (MQ=255)

            gattgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCa                                                                                   <  1:3281216/100‑1 (MQ=255)

                gattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTg                                                                               >  1:2869181/1‑100 (MQ=255)

                gattTTTATCAAGGATTACGTAGCCCTCTCTAATAGTTGCATCTTCTCCCGAGCTTCGTTCTCGGCGCGGTGGGTGAAGAGCACAGGGCGCTGGCAGTTg                                                                               <  2:1257525/100‑1 (MQ=255)

                 attTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGa                                                                              >  2:222322/1‑100 (MQ=255)

                    tttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaaa                                                                           <  2:564537/100‑1 (MQ=255)

                     ttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaaaa                                                                          >  2:2007785/1‑100 (MQ=255)

                       aTCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAAt                                                                        >  2:977699/1‑100 (MQ=255)

                       aTCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGACATTTGAAAAAAt                                                                        >  2:3128313/1‑100 (MQ=255)

                       aTCAGAGATTACGCAGCCCGCTGAAAGCTCTGCATCGGCGGCCAAGATTTTATCCCGACACGTAGGGTGAAGAGCACAGGGCCCTGGCATTTGAAAAAAt                                                                        <  2:471071/100‑1 (MQ=255)

                        tCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATg                                                                       <  1:2374586/100‑1 (MQ=255)

                        tCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATg                                                                       >  2:1250433/1‑100 (MQ=255)

                         cAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGTCGTTGATCACGACACGTTGGTTGAAGAGCGCAGTGCGCTGGCATGTGaaaaatggc                                                                      >  1:2232105/1‑96 (MQ=255)

                          agagATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                     >  1:1509251/1‑100 (MQ=255)

                           gagaTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaa                                                                    >  1:2464729/1‑100 (MQ=255)

                           gagaTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaa                                                                    >  1:1938615/1‑100 (MQ=255)

                            agaTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaaa                                                                   <  1:2683507/100‑1 (MQ=255)

                              aTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGc                                                                 <  2:2682942/100‑1 (MQ=255)

                                   gTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTttat                                                            >  1:707472/1‑100 (MQ=255)

                                   gTAGCCCTCTGAAAGAGCTGCATCTGCGGCCAGTCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTttat                                                            <  1:2031860/100‑1 (MQ=255)

                                     aGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTttatta                                                          <  1:3999183/100‑1 (MQ=255)

                                     aGCACTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTAACACGACACGTTGGGTGAAGAGCACATGGCGAAAAAAAAAAAAAAAATGCAAAGCTttatta                                                          <  2:613136/100‑1 (MQ=255)

                                      gCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACCGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAg                                                         >  2:3454610/1‑100 (MQ=255)

                                      gCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAg                                                         <  2:3225345/100‑1 (MQ=255)

                                      gCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAg                                                         >  2:786295/1‑100 (MQ=255)

                                      gCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAg                                                         <  1:1118467/100‑1 (MQ=255)

                                       cccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGt                                                        >  1:2635533/1‑100 (MQ=255)

                                         ctctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCg                                                      >  1:1998674/1‑100 (MQ=255)

                                          tctGAAAGGGCTGCCTCTGCTGCCGGGCTTTTGTCCCGACGCGTTGGGTGAAGAACACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGt                                                     <  2:2435072/100‑1 (MQ=255)

                                          tctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGt                                                     >  1:3986979/1‑100 (MQ=255)

                                            tGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGttt                                                   <  1:2667442/100‑1 (MQ=255)

                                            tGAAAGAGCTGCATCTGCTGCCAGGCATTTATAACGACAAGTTGGGTGAAGATCTCAGGGAGAAAAAAAAAAAAAAAATGCAAAGCTTTATTAGTCGttt                                                   <  2:2939164/100‑1 (MQ=255)

                                             gAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTta                                                  >  2:474847/1‑100 (MQ=255)

                                              aaaGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTtat                                                 >  2:2682129/1‑100 (MQ=255)

                                                agagCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTatat                                               >  1:1993288/1‑100 (MQ=255)

                                                agagCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTatat                                               >  2:1715016/1‑100 (MQ=255)

                                                agagCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTatat                                               >  2:1715128/1‑100 (MQ=255)

                                                 gagCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATg                                              <  1:3473779/100‑1 (MQ=255)

                                                  agCTGCATCTGCTGCCAGGCTTTTATCACGACACGGTGGGTGAAGAGCACAGGTCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGc                                             >  2:871723/1‑100 (MQ=255)

                                                    cTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                           <  1:595834/100‑1 (MQ=255)

                                                      gCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAAc                                         <  1:3195055/100‑1 (MQ=255)

                                                        aTCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACaa                                       <  1:2549593/100‑1 (MQ=255)

                                                        aTCTGCTGCCAGGCTTTTATCACGACACGCTGGGAGAAGAGCACAGGGCTCTGGCATTTGAAAACATGAAAAGCTTTATTAGCCGTTTCTATGCTAACaa                                       >  2:2385011/1‑100 (MQ=255)

                                                           tgctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATc                                    >  2:1455268/1‑100 (MQ=255)

                                                            gctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGTATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCg                                   <  2:2776501/100‑1 (MQ=255)

                                                            gctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCg                                   <  1:2439402/100‑1 (MQ=255)

                                                            gctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCg                                   >  2:2941446/1‑100 (MQ=255)

                                                              tgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCa                                 >  1:1188206/1‑100 (MQ=255)

                                                               gcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaa                                >  2:2679330/1‑100 (MQ=255)

                                                                ccAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCCTTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaa                               >  1:3336016/1‑100 (MQ=255)

                                                                ccAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaa                               <  1:2862159/100‑1 (MQ=255)

                                                                 cAGGCTTTTATCACGACACGCTGGGTCAAGAGCACAGGCCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaaa                              >  2:1738456/1‑100 (MQ=255)

                                                                  aGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaaaa                             >  2:2769891/1‑100 (MQ=255)

                                                                  aGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaaaa                             >  2:426596/1‑100 (MQ=255)

                                                                   ggCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAAt                            <  1:2310367/100‑1 (MQ=255)

                                                                   ggCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAAt                            >  1:1154594/1‑100 (MQ=255)

                                                                   ggCTTTTATCACGACACGGTGGGTGAAGAGCACAGGGCGCTGGCATTTTAAAAAATGTAAAGCTTTATTAGTCGTGTATATGCTAAGAAATCGCaaaaag                            >  2:1125972/1‑99 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                         <  1:3349471/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                         <  1:842845/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                         <  2:698849/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                         <  1:3774743/100‑1 (MQ=255)

                                                                       tttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGa                        >  1:1362690/1‑100 (MQ=255)

                                                                       tttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGa                        <  1:1964703/100‑1 (MQ=255)

                                                                        ttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGAt                       <  1:1414280/100‑1 (MQ=255)

                                                                          aTCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGATATTTTTTTTTGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTa                     >  2:1688951/1‑100 (MQ=255)

                                                                          aTCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTa                     >  1:1034897/1‑100 (MQ=255)

                                                                            cACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                   >  2:1120838/1‑100 (MQ=255)

                                                                            cACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                   >  1:3526362/1‑100 (MQ=255)

                                                                            cACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                   <  1:514604/100‑1 (MQ=255)

                                                                             aCGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCa                  >  2:718221/1‑100 (MQ=255)

                                                                             aCGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCa                  <  2:3650792/100‑1 (MQ=255)

                                                                                acacGTTGGGTGAAGAGCGCAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCa               <  2:2577014/100‑1 (MQ=255)

                                                                                acacGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCa               <  1:1288577/100‑1 (MQ=255)

                                                                                acacGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCa               <  2:1608255/100‑1 (MQ=255)

                                                                                 cacGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCAt              <  1:3645385/100‑1 (MQ=255)

                                                                                   cGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGa            >  2:296091/1‑100 (MQ=255)

                                                                                   cGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGa            >  1:3999638/1‑100 (MQ=255)

                                                                                    gTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGaa           >  1:2592382/1‑100 (MQ=255)

                                                                                      tGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGa         >  1:1253188/1‑100 (MQ=255)

                                                                                      tGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGa         <  2:2825994/100‑1 (MQ=255)

                                                                                       gggTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGAc        <  2:3964264/100‑1 (MQ=255)

                                                                                       gggTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGAc        <  1:1745742/100‑1 (MQ=255)

                                                                                        ggTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACt       >  1:2748509/1‑100 (MQ=255)

                                                                                         gTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCACAGCTTTATTAGTCGTTTATATGATAACAAATCGCAAAAATTTGATTATCAGCATGAAGTCTg      >  2:3499086/1‑100 (MQ=255)

                                                                                         gTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTCACAAATCGCAAAAATTTGATTATCAGCATGAAGACTg      <  2:3904286/100‑1 (MQ=255)

                                                                                         gTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTg      >  1:3501915/1‑100 (MQ=255)

                                                                                         gTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTg      >  1:2739376/1‑100 (MQ=255)

                                                                                          tGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGc     >  1:637372/1‑100 (MQ=255)

                                                                                          tGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGc     >  1:3575829/1‑100 (MQ=255)

                                                                                          tGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGAAAAGCTTTATTAGTCGTTTATATGCAAAAAAAAAAAAAAAATTTGATTATCAGCATGAAGACTGc     <  2:689091/100‑1 (MQ=255)

                                                                                             agagCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca  >  1:1916184/1‑100 (MQ=255)

TCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACA  >  NC_000913/1479839‑1480031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`