Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,647,986 A→C N18T (AAC→ACC)  dicA → Qin prophage; putative regulator for DicB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,647,9860AC96.0% 490.6 / 5.9 150N18T (AAC→ACC) dicAQin prophage; putative regulator for DicB
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (69/74);  total (73/77)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 158 total aligned reads displayed.

GATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAA  >  NC_000913/1647889‑1648085
                                                                                                 |                                                                                                   
gatGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAccc                                                                                                   <  2:3316942/100‑1 (MQ=255)
  tGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTc                                                                                                 <  1:50290/100‑1 (MQ=255)
   gTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCTTCa                                                                                                >  1:601165/1‑100 (MQ=255)
   gTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCa                                                                                                >  1:425884/1‑100 (MQ=255)
    ttAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCaa                                                                                               >  2:2870881/1‑100 (MQ=255)
     tAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCaaa                                                                                              >  1:3345735/1‑100 (MQ=255)
      naCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAac                                                                                             >  1:3252932/2‑100 (MQ=255)
      aaCAGAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAGAAATGTAACTATCGGCGAAGGCATCAGGTATCGTCGGAAAACCGTCAAac                                                                                             >  2:3767782/1‑100 (MQ=255)
      aaCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAac                                                                                             <  1:1036787/100‑1 (MQ=255)
      aaCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAac                                                                                             <  1:2553697/100‑1 (MQ=255)
      aaCAAAAACATAGGCAAGACGATTTATCATTATCTGACGATGGAAACAAAAAATTTAACTATCTACGAACGCATCAGGTAGCGGCGGAAAACCCTCAAac                                                                                             >  2:2002270/1‑100 (MQ=255)
       aCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAaca                                                                                            <  1:1802872/100‑1 (MQ=255)
        cAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAacac                                                                                           <  2:1233746/100‑1 (MQ=255)
        cAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAacac                                                                                           >  1:2105262/1‑100 (MQ=255)
         aaaaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAacaca                                                                                          >  2:422625/1‑100 (MQ=255)
          aaaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAacacac                                                                                         <  2:2274651/100‑1 (MQ=255)
           aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACAcc                                                                                        <  2:2434601/100‑1 (MQ=255)
             aCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCa                                                                                      <  1:3530494/100‑1 (MQ=255)
             aCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCa                                                                                      <  2:3362259/100‑1 (MQ=255)
              cATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCaa                                                                                     >  1:2341830/1‑100 (MQ=255)
                tAGTCAATACGATTTAGCATTAGCTAACTATGGAAAGAAAAAACTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCTAACGCTCCCAAAg                                                                                   <  2:3300786/100‑1 (MQ=255)
                tAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAg                                                                                   >  2:2201149/1‑100 (MQ=255)
                tAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGAGAACGCATCAGCTATCGTCGGAAAACGCTCAAACACGCCCAAAg                                                                                   >  1:3744527/1‑100 (MQ=255)
                    cAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGtct                                                                               >  1:2826125/1‑100 (MQ=255)
                     aaTACGATTTGGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAGCGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGtctc                                                                              >  2:3688436/1‑100 (MQ=255)
                          gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCt                                                                         <  1:1479413/100‑1 (MQ=255)
                           aTTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTa                                                                        >  1:3100020/1‑100 (MQ=255)
                           aTTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATAGGCGAACACATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCGTGCTa                                                                        >  1:3870838/1‑100 (MQ=255)
                              tAGCCTTAGCTAACTATGGAAACACAAAATGTAACTATCGCCGCACGCCTCAGTCATCGTCGGAAAACCCTCAACCACACCCGAAGGTCTCTTGCTAAAg                                                                     <  2:3334511/100‑1 (MQ=255)
                              tAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAg                                                                     <  1:1627125/100‑1 (MQ=255)
                              tAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAg                                                                     >  2:1715277/1‑100 (MQ=255)
                                gCATTAGCTAACTATGGAAACAAAAAATTTAACTAGCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGcc                                                                   <  2:501791/100‑1 (MQ=255)
                                  aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCt                                                                 >  1:1654257/1‑100 (MQ=255)
                                   ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTg                                                                <  1:3991166/100‑1 (MQ=255)
                                   ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTg                                                                >  1:1375001/1‑100 (MQ=255)
                                     aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa                                                              <  1:736380/100‑1 (MQ=255)
                                     aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa                                                              <  1:2225111/100‑1 (MQ=255)
                                      gCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaa                                                             >  1:3589618/1‑100 (MQ=255)
                                       cTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaaa                                                            <  2:1642404/100‑1 (MQ=255)
                                        tAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAt                                                           >  2:3026963/1‑100 (MQ=255)
                                           cTATGGAAACAAAAAATTTTACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAtctc                                                        <  2:3571226/100‑1 (MQ=255)
                                           cTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAtctc                                                        >  2:2735938/1‑100 (MQ=255)
                                            tATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTcc                                                       >  1:102936/1‑100 (MQ=255)
                                             aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTccc                                                      >  2:1455562/1‑100 (MQ=255)
                                             aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTccc                                                      <  1:2051053/100‑1 (MQ=255)
                                             aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTccc                                                      >  1:1181955/1‑100 (MQ=255)
                                              tGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCa                                                     >  2:254874/1‑100 (MQ=255)
                                               ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAt                                                    >  2:362708/1‑100 (MQ=255)
                                               ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAt                                                    >  1:765226/1‑100 (MQ=255)
                                               ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGCTCTCTTGCTAAAGCCCTGAAAATCTCCCAt                                                    >  2:1666576/1‑100 (MQ=255)
                                                gaaagaaaAAATTTAACTATCGGCGAACGCATCAGGTGTCGTCGGAAAGCCCTCAAACACGCCCAAAGGTCTCTTGCTAAAGCCCTGGAAATGTCCCAtg                                                   <  2:2932945/100‑1 (MQ=255)
                                                 aaaCAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtgt                                                  >  2:3830887/1‑100 (MQ=255)
                                                  aaCAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAtgtg                                                 <  2:1741375/100‑1 (MQ=255)
                                                   aCAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                >  1:3471758/1‑100 (MQ=255)
                                                    cAAAAAGTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAACATCTCCCATGTGTc                                               >  2:3022471/1‑100 (MQ=255)
                                                    cAAAAAATTTAACTATCGGCGAACGCATCAGGTATAGTCAGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTc                                               >  1:248116/1‑100 (MQ=255)
                                                     aaaaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTcc                                              >  2:1051744/1‑99 (MQ=255)
                                                     aaaaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCt                                              >  2:295510/1‑100 (MQ=255)
                                                     aaaaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCt                                              >  1:1291343/1‑100 (MQ=255)
                                                      aaaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTg                                             <  2:3085349/100‑1 (MQ=255)
                                                       aaaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGt                                            >  2:2262131/1‑100 (MQ=255)
                                                        aaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTa                                           <  1:4064664/100‑1 (MQ=255)
                                                        aaaTTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTa                                           <  1:3775529/100‑1 (MQ=255)
                                                           tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATca                                        <  2:556786/100‑1 (MQ=255)
                                                           tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATca                                        >  2:2529929/1‑100 (MQ=255)
                                                           tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATca                                        <  1:3112739/100‑1 (MQ=255)
                                                               aCTATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAAt                                    <  1:3201493/100‑1 (MQ=255)
                                                                 tATCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATgg                                  <  2:2695886/100‑1 (MQ=255)
                                                                  aTCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACATACTCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATggg                                 <  2:3812531/100‑1 (MQ=255)
                                                                  aTCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATggg                                 <  1:2388148/100‑1 (MQ=255)
                                                                  aTCGGCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATggg                                 <  2:3914331/100‑1 (MQ=255)
                                                                     ggCGAACGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAAc                              >  1:2569738/1‑100 (MQ=255)
                                                                         aaCGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgggg                          >  1:3052992/1‑100 (MQ=255)
                                                                          aCGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGt                         <  1:1289286/100‑1 (MQ=255)
                                                                          aCGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGt                         >  2:626598/1‑100 (MQ=255)
                                                                           cGCATCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTg                        >  1:1171203/1‑100 (MQ=255)
                                                                              aTCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATa                     >  1:1464078/1‑100 (MQ=255)
                                                                              aTCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATa                     >  1:263375/1‑100 (MQ=255)
                                                                               tCAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAg                    <  1:2454549/100‑1 (MQ=255)
                                                                                cAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGt                   <  1:3879767/100‑1 (MQ=255)
                                                                                cAGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGt                   <  2:3208774/100‑1 (MQ=255)
                                                                                 aGGTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACGATGGGAACGGGGTGATAGTg                  <  2:2072634/100‑1 (MQ=255)
                                                                                  ggTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGa                 <  1:2446644/100‑1 (MQ=255)
                                                                                   gTATCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGaa                <  2:2607153/100‑1 (MQ=255)
                                                                                    tataGTCGGAAAATCGTCAAACACAGCCAATGGTCTCCTGCTCAAGCCCTGACACTCTCCCATGTGTCTGTATGACAATGGGCACGGGGTGATAGTGacc               <  2:1058177/100‑3 (MQ=255)
                                                                                     aTCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAAcc              <  1:2100958/100‑1 (MQ=255)
                                                                                     aTCGTCGGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAAcc              <  2:235922/100‑1 (MQ=255)
                                                                                       cgtcgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTa            >  1:663871/1‑100 (MQ=255)
                                                                                       cgtcgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTa            >  1:2055314/1‑100 (MQ=255)
                                                                                       cgtcgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTa            >  1:3320851/1‑100 (MQ=255)
                                                                                        gtcgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTAc           >  2:2573893/1‑100 (MQ=255)
                                                                                          cgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGTCCTGAAACTCTCCCATGTGTCTGTATCACTATGGGAACGGGGTGATAGTGAACCTACAg         <  2:2500817/100‑1 (MQ=255)
                                                                                          cgGAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAg         <  1:169888/100‑1 (MQ=255)
                                                                                           ngAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAgg        >  1:3254660/2‑100 (MQ=255)
                                                                                           ggAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAgg        <  2:3442187/100‑1 (MQ=255)
                                                                                           ggAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAgg        >  1:2753919/1‑100 (MQ=255)
                                                                                           ggAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAgg        >  2:868353/1‑100 (MQ=255)
                                                                                            gAAAACCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAggg       >  1:20089/1‑100 (MQ=255)
                                                                                             aaaacCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGga      >  2:314945/1‑100 (MQ=255)
                                                                                             aaaacCCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGga      <  2:1580652/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAA  >  NC_000913/1647889‑1648085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑