Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,823,297 T→G intergenic (+12/‑218) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,823,2970TG93.6% 527.4 / 14.6 172intergenic (+12/‑218)nadE/choNAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (3/8);  new base G (66/95);  total (69/103)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

CCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAG  >  NC_000913/1823200‑1823392
                                                                                                 |                                                                                               
     aCAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGAt                                                                                          <  1:2758058/100‑1 (MQ=255)
     aCAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGAt                                                                                          <  1:3273883/100‑1 (MQ=255)
      cAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGAtt                                                                                         >  2:3198547/1‑100 (MQ=255)
         tAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATtgtg                                                                                      <  2:2348910/100‑1 (MQ=255)
           gagaACTGGTATCTGAAAACCGCACATCGCCGCCGTCCGCCAATTGCCGCTTTCGAACATTTCAGGAAAAAGCAAAAATTTGCACAGACTGGGTTgcgcc                                                                                    >  2:3873499/1‑99 (MQ=255)
           gagaACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCg                                                                                    <  1:2353118/100‑1 (MQ=255)
                 tGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAag                                                                              <  1:4019702/100‑1 (MQ=255)
                  ggTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAaga                                                                             >  1:3040640/1‑100 (MQ=255)
                  ggTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAaga                                                                             >  1:898372/1‑100 (MQ=255)
                  ggTATCTGAAAACCGAACATAAACGCCGTCCG‑CAATTACCGTTTTCGATGATATCTCGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAagag                                                                            <  2:1960691/100‑1 (MQ=255)
                     aTCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTa                                                                          >  1:2820430/1‑100 (MQ=255)
                      tCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTaa                                                                         >  1:2176025/1‑100 (MQ=255)
                        tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAAtt                                                                       >  2:3577945/1‑100 (MQ=255)
                        tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAAtt                                                                       >  1:1349682/1‑100 (MQ=255)
                        tGAAAACCGAACATAAACGCCGACCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAAtt                                                                       <  2:1315128/100‑1 (MQ=255)
                         gAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAAttt                                                                      >  2:3717561/1‑100 (MQ=255)
                          aaaaCCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTg                                                                     <  2:583090/100‑1 (MQ=255)
                           aaaCCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGt                                                                    >  1:3491294/1‑100 (MQ=255)
                           aaaCCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGt                                                                    <  2:1391279/100‑1 (MQ=255)
                              ccGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCa                                                                 <  1:1423696/100‑1 (MQ=255)
                                gAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACg                                                               <  1:2425435/100‑1 (MQ=255)
                                   cATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCg                                                            >  1:2640333/1‑100 (MQ=255)
                                     tAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGa                                                          >  1:1702502/1‑100 (MQ=255)
                                      aagcGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGAt                                                         <  1:1171794/97‑1 (MQ=255)
                                       aaCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATg                                                        >  2:2520359/1‑100 (MQ=255)
                                        aCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACCGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATgc                                                       >  1:2698502/1‑100 (MQ=255)
                                        aCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATgc                                                       <  2:13563/100‑1 (MQ=255)
                                          gCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATgcgg                                                     <  1:1601963/100‑1 (MQ=255)
                                             gTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGt                                                  >  2:516001/1‑100 (MQ=255)
                                             gTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGt                                                  >  1:3950926/1‑100 (MQ=255)
                                              tCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTg                                                 <  2:711135/100‑1 (MQ=255)
                                               ccGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGa                                                <  1:758214/100‑1 (MQ=255)
                                               ccGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGa                                                >  1:1612533/1‑100 (MQ=255)
                                               ccGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGa                                                <  2:2777833/100‑1 (MQ=255)
                                                ctccAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGaa                                               <  2:2441539/98‑1 (MQ=255)
                                                cGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATCTTGGATTGTGCGAAAAAGAGCAATTTGTTCACGCCGGATGCGGCGTGaa                                               >  2:2057936/1‑100 (MQ=255)
                                                 gCCAATTTCCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGatc                                              <  2:3706180/100‑3 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATCATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                              <  1:3899718/100‑1 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                              <  2:2707401/100‑1 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                              >  2:3872982/1‑100 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                              <  2:563900/100‑1 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                              >  2:96260/1‑100 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAc                                              <  2:2899406/100‑1 (MQ=255)
                                                 gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAc                                              <  2:2018683/100‑1 (MQ=255)
                                                 gCCAATTACCGCTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCTGCGTGAAc                                              <  2:617940/100‑1 (MQ=255)
                                                  ccAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGaaag                                             >  1:2479651/1‑98 (MQ=255)
                                                  ccAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAca                                             <  1:3367391/100‑2 (MQ=255)
                                                    aaTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                           <  2:1770158/100‑1 (MQ=255)
                                                    aaTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                           <  1:1145957/100‑1 (MQ=255)
                                                    aaTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                           <  2:1582441/100‑1 (MQ=255)
                                                    aaTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATAGAAAAAAAAAAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                           <  2:2702003/100‑1 (MQ=25)
                                                     aTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCt                                          <  1:545315/100‑1 (MQ=255)
                                                     aTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCt                                          <  1:1241141/100‑1 (MQ=255)
                                                     aTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCt                                          <  2:410291/100‑1 (MQ=255)
                                                     aTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCt                                          >  2:1024653/1‑100 (MQ=255)
                                                      ttACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAcaaatt                                         <  1:1833729/100‑6 (MQ=38)
                                                      ttACCGTGTTCGATGATCTCTGGAAAAAGTAATAATTTGCACAGGTTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAcaaatt                                         <  1:3751176/100‑6 (MQ=37)
                                                       tACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCttn                                        <  1:3253246/100‑2 (MQ=255)
                                                        aCCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTAt                                       <  2:575230/100‑1 (MQ=255)
                                                         ccGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTAtt                                      <  1:1847673/100‑1 (MQ=255)
                                                         ccGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTAtt                                      >  1:624667/1‑100 (MQ=255)
                                                           gTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCg                                    >  1:1886638/1‑100 (MQ=255)
                                                             tttCGATGATTTCTAAAAAAAGAAATAATTTACACAGATTGGATTGTGAGAAAAAGAGTAATCGGTTCACGCCGGATGCGGCGTGAACGCCTTACTCGAc                                  <  2:3752592/100‑1 (MQ=37)
                                                              ttCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAcc                                 <  1:173580/100‑1 (MQ=255)
                                                              ttCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAcc                                 <  2:2337090/100‑1 (MQ=255)
                                                              ttCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAcc                                 <  1:79974/100‑1 (MQ=255)
                                                               tCGATGATTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCt                                <  2:1132730/100‑1 (MQ=255)
                                                                cgacgaCTTCTGGAAAAAGTAATAATAGGCACAGAATGGAGTGGGCGAAAAAGAGAAATTTCTTCACGCAGGATACGGCGTGAACGACTTATTCGACAta                               >  2:1379130/1‑100 (MQ=255)
                                                                cGATGATTTCTGGAAAAAGGAATAATTTGCAGAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCta                               <  2:909284/100‑1 (MQ=255)
                                                                 gatgatTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCtat                              >  1:3694254/1‑100 (MQ=255)
                                                                  atggtTTCTGGAAAAAGTAATAATGTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTGTTCGACCgata                             <  2:34023/100‑1 (MQ=38)
                                                                  atgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCtata                             <  2:1540698/100‑1 (MQ=255)
                                                                  atgatTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCtata                             <  1:1735078/100‑1 (MQ=255)
                                                                    gatTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaa                           >  1:587116/1‑100 (MQ=255)
                                                                    gatTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaa                           <  2:297336/100‑1 (MQ=255)
                                                                    gatTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaa                           <  2:713712/100‑1 (MQ=255)
                                                                     atTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaaa                          <  1:2134365/100‑1 (MQ=255)
                                                                     atTTCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaaa                          >  1:3053659/1‑100 (MQ=255)
                                                                      tttCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAAc                         >  1:3793071/1‑100 (MQ=255)
                                                                        tCTGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTa                       <  2:1658535/100‑1 (MQ=255)
                                                                        tCTGGAAAAAGTAATAAGTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGGCTTATTCGACCTATAAAACTa                       <  2:243598/100‑1 (MQ=255)
                                                                          tGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTCTg                     >  1:1183665/1‑100 (MQ=255)
                                                                          tGGAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATg                     >  2:2028495/1‑100 (MQ=255)
                                                                           ggAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGc                    <  2:2657440/100‑1 (MQ=255)
                                                                           ggAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGc                    >  1:3102330/1‑100 (MQ=255)
                                                                           ggAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGc                    >  1:1857442/1‑100 (MQ=255)
                                                                            gAAAAAGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCa                   <  1:1011049/100‑1 (MQ=255)
                                                                              aaaaGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCaaa                 >  1:2056302/1‑100 (MQ=255)
                                                                               aaaGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCTACCTATAAAACTATGCAAAt                >  2:2882113/1‑100 (MQ=255)
                                                                                 aGTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTc              >  2:1715015/1‑100 (MQ=255)
                                                                                  gTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCa             <  2:3089422/100‑1 (MQ=255)
                                                                                  gTAATAATTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCa             >  1:315610/1‑100 (MQ=255)
                                                                                   taataaTTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCaa            <  1:3301581/100‑1 (MQ=255)
                                                                                    gagaatTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTGCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAAt           <  1:3216879/97‑1 (MQ=255)
                                                                                    aataatTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAAt           <  1:1025868/100‑1 (MQ=255)
                                                                                    aataatTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAAt           <  1:3319876/100‑1 (MQ=255)
                                                                                     ataatTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATa          <  1:2551715/100‑1 (MQ=255)
                                                                                        atTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAt       <  2:3284096/100‑1 (MQ=255)
                                                                                        atTTGCACAGATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAt       <  2:1261925/100‑1 (MQ=255)
                                                                                            gCACAGATTGGATTGTGAGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCa   <  2:1621102/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAG  >  NC_000913/1823200‑1823392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑