Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,024,185 A→C N73T (AAT→ACT)  rcsA → transcriptional regulator of colanic acid capsular biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,1850AC92.6% 453.1 / 20.7 150N73T (AAT→ACT) rcsAtranscriptional regulator of colanic acid capsular biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base C (70/67);  total (78/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 151 total aligned reads displayed.

ATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAGTTCTAA  >  NC_000913/2024088‑2024284
                                                                                                 |                                                                                                   
aTAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTc                                                                                                   <  2:1761394/100‑1 (MQ=255)
 tAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCa                                                                                                  <  1:2131829/100‑1 (MQ=255)
  aGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCaa                                                                                                 <  2:3534519/100‑1 (MQ=255)
     ttGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGGGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACAt                                                                                              <  2:333530/100‑1 (MQ=255)
      tgtgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATc                                                                                             >  2:2263279/1‑100 (MQ=255)
        tgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATccc                                                                                           <  2:1293623/100‑1 (MQ=255)
          aTTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATTCCaa                                                                                         >  1:3657882/1‑100 (MQ=255)
          aTTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCaa                                                                                         >  1:3317760/1‑100 (MQ=255)
           ttCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAAt                                                                                        <  1:2558071/100‑1 (MQ=255)
           ttCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAAt                                                                                        >  2:2518560/1‑100 (MQ=255)
           ttCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAAt                                                                                        <  2:3172370/100‑1 (MQ=255)
           gtCACAGCGCCCTGCAGTGGGGTTTATGAATGAGGACTGTTTCATCCACGATGCTTCAAACATGCAGCTTATCAAGCTCATCAGTACTCAACATCCCAAt                                                                                        >  2:3996203/2‑100 (MQ=255)
            tCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATa                                                                                       >  2:3855327/1‑100 (MQ=255)
               caGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGt                                                                                    >  1:789121/1‑100 (MQ=255)
                  cgcCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGttat                                                                                 <  2:1096028/100‑1 (MQ=255)
                   gcCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGttatt                                                                                <  2:2348036/100‑1 (MQ=255)
                        tCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATcn                                                                           <  1:1363081/100‑2 (MQ=255)
                        tCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCg                                                                           <  2:559733/100‑1 (MQ=255)
                         cAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGt                                                                          <  1:1442804/100‑1 (MQ=255)
                         cAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGt                                                                          <  2:1212007/100‑1 (MQ=255)
                              gtgtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTa                                                                     <  1:2776989/100‑1 (MQ=255)
                               tgtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTAt                                                                    >  2:1468341/1‑100 (MQ=255)
                                gtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATg                                                                   >  1:3985343/1‑100 (MQ=255)
                                gtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATg                                                                   >  1:404804/1‑100 (MQ=255)
                                gtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATg                                                                   >  2:46771/1‑100 (MQ=255)
                                gtTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATg                                                                   >  1:959871/1‑100 (MQ=255)
                                   tattaATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCa                                                                >  2:3798104/1‑100 (MQ=255)
                                     ttaATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAAt                                                              >  1:935840/1‑100 (MQ=255)
                                      taATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAAtt                                                             >  1:147754/1‑100 (MQ=255)
                                        aTGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGc                                                           >  2:2393136/1‑100 (MQ=255)
                                          gAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTTTGGCAATTGCCa                                                         <  1:3516542/100‑1 (MQ=255)
                                           aGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCaa                                                        <  1:3854700/100‑1 (MQ=255)
                                            ggACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAAt                                                       >  1:1188356/1‑100 (MQ=255)
                                             gACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATg                                                      >  1:98600/1‑100 (MQ=255)
                                               cTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGtt                                                    >  2:3149530/1‑100 (MQ=255)
                                                tGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTc                                                   <  2:1557519/100‑1 (MQ=255)
                                                 gTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTcn                                                  <  1:3239442/100‑2 (MQ=255)
                                                 gTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCa                                                  >  1:1373142/1‑100 (MQ=255)
                                                 gTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCa                                                  >  1:2506854/1‑100 (MQ=255)
                                                   ttCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCAtt                                                <  1:1087127/100‑1 (MQ=255)
                                                     cATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCAtttt                                              >  1:2557650/1‑100 (MQ=255)
                                                      aTCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtg                                             >  1:368039/1‑100 (MQ=255)
                                                        ccACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCATTTGCCAATGTTGATTTtgat                                           >  1:1840363/1‑100 (MQ=255)
                                                        ccACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtgat                                           >  1:805068/1‑100 (MQ=255)
                                                        ccACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtgat                                           <  2:2995052/100‑1 (MQ=255)
                                                         cACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtgatg                                          <  1:1270720/100‑1 (MQ=255)
                                                           cGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGaa                                        <  2:2105021/100‑1 (MQ=255)
                                                            gATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAat                                       <  1:3497598/100‑1 (MQ=255)
                                                                cTTCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCt                                   >  2:3950907/1‑100 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCTAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAt                                 <  1:3154761/100‑1 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAt                                 >  2:2893858/1‑100 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAt                                 <  2:2415959/100‑1 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCAAGCTCATCATTACCCAACATCCCAATACGTTATATATCGCTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAt                                 >  2:889495/1‑100 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGACTATCtcc                                 >  1:629410/1‑98 (MQ=255)
                                                                  tCTAACAGTCAGCGTATCAAACTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAt                                 >  1:566401/1‑100 (MQ=255)
                                                                   cTAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATATAtt                                >  2:3606731/1‑100 (MQ=255)
                                                                   cTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTAtt                                <  1:1914048/100‑1 (MQ=255)
                                                                    tAACAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTg                               <  1:1012447/100‑1 (MQ=255)
                                                                     aaCAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTgg                              <  2:1301976/100‑1 (MQ=255)
                                                                     aaCAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTgg                              <  1:1301368/100‑1 (MQ=255)
                                                                       cAGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTc                            <  1:2675426/100‑1 (MQ=255)
                                                                        aGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCa                           >  2:1598834/1‑100 (MQ=255)
                                                                        aGTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCa                           <  2:3035580/100‑1 (MQ=255)
                                                                         gTCAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAg                          <  2:1978702/100‑1 (MQ=255)
                                                                           ctgtttATCAAGCTCATCAGTACTCATAATCCCAACACGTTATTTATCGTTTTGATGGCGATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaa                        <  2:514527/95‑1 (MQ=255)
                                                                           cAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGGCAATGTTCATTTTGATGAATATTTATTGGTTAGaa                        >  2:3368830/1‑100 (MQ=255)
                                                                           cAGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaa                        <  1:1144555/100‑1 (MQ=255)
                                                                            aGCGTATCAAGCTCATCATTACTCAACATCCCAATAGGTTGTTTATCGTTTTTACGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaa                       >  2:3754425/1‑100 (MQ=255)
                                                                            aGCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaa                       >  2:3545507/1‑100 (MQ=255)
                                                                             gCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaaa                      >  1:1144162/1‑100 (MQ=255)
                                                                             gCGTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaaa                      >  2:2798015/1‑100 (MQ=255)
                                                                               gTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaaaaa                    >  1:1623411/1‑100 (MQ=255)
                                                                               gTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaaaaa                    <  1:3781336/100‑1 (MQ=255)
                                                                               gTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCAAATGTTCATTTTGATGAATATCTATTGGTCAGaaaaaa                    <  2:1091584/100‑1 (MQ=255)
                                                                               gTATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTACGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGaaaaaa                    >  1:4050669/1‑100 (MQ=255)
                                                                                tATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAt                   <  1:2430440/100‑1 (MQ=255)
                                                                                tATCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAt                   <  1:3784779/100‑1 (MQ=255)
                                                                                 aTCAAGCTCATCATTATTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAtt                  >  2:3865831/1‑100 (MQ=255)
                                                                                 aTCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAtt                  >  2:2575566/1‑100 (MQ=255)
                                                                                 aTCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAtt                  >  2:3050893/1‑100 (MQ=255)
                                                                                  tCAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAttt                 <  2:1163430/100‑1 (MQ=255)
                                                                                   cAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa                <  1:1704573/100‑1 (MQ=255)
                                                                                   cAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa                <  1:2011770/100‑1 (MQ=255)
                                                                                   cAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa                <  1:2727646/100‑1 (MQ=255)
                                                                                   cAAGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa                >  1:1683292/1‑100 (MQ=255)
                                                                                    aaGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTTCCAATGTTCATTTTGATGAATATCTATTGGTGAGAAAAGATTTAt               >  2:810271/1‑100 (MQ=255)
                                                                                    aaGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAt               >  2:1636619/1‑100 (MQ=255)
                                                                                     aGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAtt              >  2:266231/1‑100 (MQ=255)
                                                                                     aGCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAtt              <  1:2249483/100‑1 (MQ=255)
                                                                                      gCTCATCATTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTg             <  2:2953128/100‑1 (MQ=255)
                                                                                         catcatTACTTAACATCCCAATACGTTAATTGACGTTGTACCGGCAATTCCCATAGTGCATTTTGTTGCCTCTCTATCGATCAGTTACAATTTATTGATc          >  2:785563/1‑100 (MQ=255)
                                                                                         catcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATc          >  1:2740468/1‑100 (MQ=255)
                                                                                         catcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATc          <  1:3478277/100‑1 (MQ=255)
                                                                                         catcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATc          >  2:686749/1‑100 (MQ=255)
                                                                                           tcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAg        <  1:1295585/100‑1 (MQ=255)
                                                                                           tcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAg        <  2:165425/100‑1 (MQ=255)
                                                                                           tcatTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAg        <  2:641467/100‑1 (MQ=255)
                                                                                            catTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAGt       >  1:164300/1‑100 (MQ=255)
                                                                                             atTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAGtt      <  1:2005808/100‑1 (MQ=255)
                                                                                             atTACTCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAGtt      >  2:2208291/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
ATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTATTGATCAGTTCTAA  >  NC_000913/2024088‑2024284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: