Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 75,023 | 0 | A | C | 34.2% | 127.6 / 17.2 | 73 | V153G (GTG→GGG) | thiB | thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein |
Reads supporting (aligned to +/- strand): ref base A (30/18); new base C (0/25); total (30/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.94e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
ATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCAC > NC_000913/74794‑75249 | aTCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGt > 1:1433095/1‑239 (MQ=255) tctcttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATc > 2:1602822/1‑238 (MQ=255) ttttcGAGAATGGTAAAGGCCGGAGGGGTGGTGTAACGCAGTACCAGATCGCTTTCACTTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGTCTTTTTGCCTCCATAGCAACAGACCCAGCCCCGGTGTCCTGGTGCGCGGATCCTG‑AAAAATCACCCGCCAGTTTTGATCGct < 2:1702842/237‑1 (MQ=255) cttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATc > 1:1566522/1‑235 (MQ=255) tgtgATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCCTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGt > 1:1847094/1‑239 (MQ=255) gtgAAAAGGCGGAGGGGTGGTGTACCCCGGACCCGGTACGCTTCCCCCGTTTAAAACAAGGCC‑GTAGGTTGCCTCCCAGCCTTGGGTGCCGGTGGCCTTTTTCTTCGCCATTTTCTGCCAGGCTTGTGGGGCGTCA‑GCGCCATATATTTTTGGCATCCATGGCAACAGACCCAGCCCCGTTGTACGGGTGCGCTGACCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAg < 2:1831006/237‑1 (MQ=255) aGCCGGAGGGGTGGTCTACCTCAGTACAAGATCGCTTTCACCTTTTAAAACCAGCCC‑GTAGGCTTCGCTCCAGCTTTTGGTGACCGTGACCTTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGAt < 2:534278/218‑1 (MQ=255) agagGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCttt > 2:1000372/1‑239 (MQ=255) gtggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAAc > 1:534278/1‑218 (MQ=255) gggttaaccTCAGTCCCAGTTC‑CTTTTCCCTTTTTAAAACCGGCCGGTGGCCTTCGCCCCAGCCTTTGGTGCCCGTGACCTTTTTCTCCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGAt < 2:1717316/199‑1 (MQ=255) ggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGATCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCg > 1:1811846/1‑239 (MQ=255) tAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtt > 1:1222294/1‑239 (MQ=255) cAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGATTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAAc > 1:1717316/1‑207 (MQ=255) gTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCgggggttttc > 1:182842/1‑230 (MQ=255) gATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg < 1:678090/202‑1 (MQ=255) gATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg > 2:678090/1‑202 (MQ=255) aaTCTCTTGCCCCTTTAAAAAACGGGCC‑GTAGTCTTCCCCCCAGCCTTTGGTGACCGGCCCCGTTTTCTTCGCCAGTTTCGGCCGGGCTTGGGGGGCGTCA‑TCGCCATAGCCTTTTTGCATCCATAGCAACAGACCCACCCCCGGTGTACTGGTGCGCGGTTCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc < 2:1222294/238‑1 (MQ=255) tCGCTTTCCCCTTTAAAAAACAGGCC‑GTAGGCTTCGCTCCAGCTTTGGGTGGCCGTGACCGTTTTCTTCGCCAGTTTCGGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGGGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc < 2:871980/237‑1 (MQ=255) gttttCCCCTTTAAAAAACGGGCC‑GTAGGCTTCCCCCCAGCCTTTGGTGACCGTGCCCTTTTTTTTCGCCAGTTTCTGCCAGGCTTGGGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg < 2:1192224/213‑1 (MQ=255) cTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGAt > 2:782820/1‑184 (MQ=255) cTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGAt < 1:782820/184‑1 (MQ=255) cTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTAGtttttt > 1:1252519/1‑233 (MQ=255) cTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTttttt > 1:617657/1‑236 (MQ=255) cTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTGTTTGTttttt > 1:918755/1‑236 (MQ=255) cTTCCACCTTTTAAAAACGGCCC‑GTAGGCTTCGCTCCAGCTTTGGGTGACCGTGACCTTTTTCTTCGCCAGTTTCTGCCAGGCTTGGGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTc < 2:1847094/239‑1 (MQ=255) aacggccc‑GTGGGCTTCGCCCAAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCATTTTCTGCCGGGCTTGTGGGGCGTCA‑TCGCCATAGCCTTTTTGCATCAATACCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGAt < 2:1452761/163‑1 (MQ=255) agtggCC‑GTAGCCTCCCCCCCAGCTTTGGGTGACCGTGCCCTTTTTCTTCGCCGGTTTCGGCCCGGCTTGGGGGGCGTCT‑TCGCCATAGCCTTTTTGAATCAATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGTTCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAg < 2:586981/236‑1 (MQ=255) gCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGa > 2:134422/1‑163 (MQ=255) gCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGa < 1:134422/163‑1 (MQ=255) cc‑GTATGTTTCGCCCCAGCTTTGGGTGCCCGTGCCCTTTTTCTTCGCCGTTTTCTGCCAGGTTTGGGGGGCGTCA‑GCCCCATAGACTTTTTGCCCCAATAGCAACAGCCCCAGCCCGGGTGTACGGGTGCGCGGTTCCTG‑AAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGc < 2:617657/238‑1 (MQ=255) aGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAAc > 1:1452761/1‑169 (MQ=255) gCTTCGCTCCACCCTTTGGTGACGGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTGGTGGGGCGTCA‑TCGCCATAGCCTTTTTGCACCCATAGCAACAGACCCAGCCCCGGTGTACTGGTCCGCGGACCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg < 1:1559441/170‑1 (MQ=255) tCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa < 1:1176802/239‑1 (MQ=255) cggcccccgCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGTTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa < 1:1358251/231‑1 (MQ=255) ccGCTCCAGCTTTTGGTGACCGTACCCGTTTCCTTGCCCATTTCCGGCCAGCCTT‑TGGGGGGTTACGCCCCATGGCTTTTTGGCACCCAAAGCAACAGCCCCAGCCCCGGGGTACTGGTGCCCGGTTCCTG‑ATAAAGCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa < 2:1811846/238‑1 (MQ=255) cGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc > 2:634438/1‑185 (MQ=255) cGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc < 1:634438/185‑1 (MQ=255) aGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGGGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAgg > 2:1559441/1‑170 (MQ=255) ttgggaaccgtgactgtTTTTTACGCCAGTTTCTGCCGGGCTGGGGGGGCGCCA‑TCGCCATATCTTTTTTCCACCCATAGCACCGGCCCCATCCCCGTTTTGCTGG‑GGGCGGGTCCTGAAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTCCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgt < 2:1306344/233‑1 (MQ=255) ggTGACCGTGACTTTTTTCTTCGCCATTTTCGGCCAGGCTTGGGGGGCGTAT‑GCGCCATAGTTTTTTGGCCACCATAGCACCAGCCCCGGCCCCGGGGTCCTGGGGCGGGTATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgtgt < 2:1252519/239‑1 (MQ=255) cccgtgacagtTTTCTTCGCTAGTTTCGCCCAGGGTTGGGGGGAGTCA‑TCCCCATAGTCTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTTTACTGGTGCGCGGATCCTA‑AAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGaa < 2:730440/205‑1 (MQ=255) ttttCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg > 2:291368/1‑139 (MQ=255) ttttCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg < 1:291368/139‑1 (MQ=255) cGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGGTTTTTTTTTTTGTAAAAAAAAGCGAAGTAAGCAGAATCAAACAGTACGAAAAAGTCATTTTTTCAACCCCCgggg > 2:769128/1‑237 (MQ=255) ccAGGCTTGGGGGGGGTCA‑TCGCCATAGACTTTTTGCACCCATAGCAACAGCCCCAGCCCCGGTGTACGGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg < 2:1530163/137‑1 (MQ=255) ttGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTGTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAgg < 1:1738942/167‑1 (MQ=255) ggggCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgtttt > 1:1530163/1‑137 (MQ=255) tCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGtt > 1:1360153/1‑86 (MQ=255) ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc < 1:233208/96‑1 (MQ=255) ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc > 2:233208/1‑96 (MQ=255) tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGAt < 1:987822/69‑1 (MQ=255) tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGAt > 2:987822/1‑69 (MQ=255) cAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAAc < 1:294416/189‑1 (MQ=255) aaCAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTGGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCg < 1:1796289/129‑1 (MQ=255) aCAGCCCCAGCCCGGGTGTACTGGTGCGCGGATCCTA‑AAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg < 1:759297/178‑1 (MQ=255) gACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgt < 1:1047964/154‑1 (MQ=255) cggCCCCGGTGTAGTGGTGCGCGGACCCTA‑AAAAACCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACa < 1:427139/237‑1 (MQ=255) aGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTCATAAAAGAAAGGGAAGTAGGCATAAACAAAACGTACGAAAAGGTCATTATTTCCGCCCCCCGGGACGTTAACCGGCTCCGCt > 2:294416/1‑189 (MQ=255) tgtACTGGTGCGCGGATCCTT‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCtt < 1:977228/193‑1 (MQ=255) tatccTGGTGCGCGGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGTTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa < 1:1347568/159‑1 (MQ=255) tcttGGTGCGGGGTCCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc < 1:35933/236‑1 (MQ=255) tccTGGTGGGCGGATCCTA‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc < 1:1309563/237‑1 (MQ=255) tACTGGTGCGCGGTTCCTG‑AAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGc < 2:522185/117‑1 (MQ=255) cTGGTGCGCGGTTCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCa < 2:1581895/82‑1 (MQ=255) cTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtttttta > 1:1581895/1‑80 (MQ=255) gcgcGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg > 2:397709/1‑48 (MQ=255) gcgcGGATCCTG‑ATAAACCACCCGCCAGTTTTGATCGCTCTCAACCAg < 1:397709/48‑1 (MQ=255) cgcgGATCCTG‑ATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAg < 2:376649/47‑1 (MQ=255) cgcgGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg > 1:376649/1‑47 (MQ=255) gcgGACCCTG‑AAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCATTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTACAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGccc < 1:1313113/235‑1 (MQ=255) cgGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTCTTTTTTTCTTTTTATTAACGAAGGCGGAGAAGCCCCAATTaaa > 1:522185/1‑117 (MQ=255) aTCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACg < 1:731201/116‑1 (MQ=255) tCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGcaccac < 1:1000372/239‑1 (MQ=255) | ATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCC‑GTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCA‑TCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTG‑ATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCAC > NC_000913/74794‑75249 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |