Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091375,0230AC34.2% 127.6 / 17.2 73V153G (GTG→GGG) thiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (30/18);  new base C (0/25);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 2.94e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCAC  >  NC_000913/74794‑75249
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  
aTCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGt                                                                                                                                                                                                                           >  1:1433095/1‑239 (MQ=255)
        tctcttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATc                                                                                                                                                                                                                    >  2:1602822/1‑238 (MQ=255)
          ttttcGAGAATGGTAAAGGCCGGAGGGGTGGTGTAACGCAGTACCAGATCGCTTTCACTTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGTCTTTTTGCCTCCATAGCAACAGACCCAGCCCCGGTGTCCTGGTGCGCGGATCCTGAAAAATCACCCGCCAGTTTTGATCGct                                                                                                                                                                                                                 <  2:1702842/237‑1 (MQ=255)
           cttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATc                                                                                                                                                                                                                    >  1:1566522/1‑235 (MQ=255)
                    tgtgATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCCTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGt                                                                                                                                                                                                       >  1:1847094/1‑239 (MQ=255)
                     gtgAAAAGGCGGAGGGGTGGTGTACCCCGGACCCGGTACGCTTCCCCCGTTTAAAACAAGGCCGTAGGTTGCCTCCCAGCCTTGGGTGCCGGTGGCCTTTTTCTTCGCCATTTTCTGCCAGGCTTGTGGGGCGTCAGCGCCATATATTTTTGGCATCCATGGCAACAGACCCAGCCCCGTTGTACGGGTGCGCTGACCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  2:1831006/237‑1 (MQ=255)
                           aGCCGGAGGGGTGGTCTACCTCAGTACAAGATCGCTTTCACCTTTTAAAACCAGCCCGTAGGCTTCGCTCCAGCTTTTGGTGACCGTGACCTTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                     <  2:534278/218‑1 (MQ=255)
                                 agagGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCttt                                                                                                                                                                                          >  2:1000372/1‑239 (MQ=255)
                                     gtggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAAc                                                                                                                                                                                                           >  1:534278/1‑218 (MQ=255)
                                      gggttaaccTCAGTCCCAGTTCCTTTTCCCTTTTTAAAACCGGCCGGTGGCCTTCGCCCCAGCCTTTGGTGCCCGTGACCTTTTTCTCCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGAt                                                                                                                                                                                                                     <  2:1717316/199‑1 (MQ=255)
                                       ggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGATCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCg                                                                                                                                                                                    >  1:1811846/1‑239 (MQ=255)
                                           tAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtt                                                                                                                                                                                >  1:1222294/1‑239 (MQ=255)
                                                cAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGATTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAAc                                                                                                                                                                                                           >  1:1717316/1‑207 (MQ=255)
                                                  gTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCgggggttttc                                                                                                                                                                           >  1:182842/1‑230 (MQ=255)
                                                        gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  1:678090/202‑1 (MQ=255)
                                                        gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        >  2:678090/1‑202 (MQ=255)
                                                        aaTCTCTTGCCCCTTTAAAAAACGGGCCGTAGTCTTCCCCCCAGCCTTTGGTGACCGGCCCCGTTTTCTTCGCCAGTTTCGGCCGGGCTTGGGGGGCGTCATCGCCATAGCCTTTTTGCATCCATAGCAACAGACCCACCCCCGGTGTACTGGTGCGCGGTTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc                                                                                                                                                                   <  2:1222294/238‑1 (MQ=255)
                                                          tCGCTTTCCCCTTTAAAAAACAGGCCGTAGGCTTCGCTCCAGCTTTGGGTGGCCGTGACCGTTTTCTTCGCCAGTTTCGGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGGGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTc                                                                                                                                                                   <  2:871980/237‑1 (MQ=255)
                                                            gttttCCCCTTTAAAAAACGGGCCGTAGGCTTCCCCCCAGCCTTTGGTGACCGTGCCCTTTTTTTTCGCCAGTTTCTGCCAGGCTTGGGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg                                                                                                                                                                                       <  2:1192224/213‑1 (MQ=255)
                                                             cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                     >  2:782820/1‑184 (MQ=255)
                                                             cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                     <  1:782820/184‑1 (MQ=255)
                                                             cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTAGtttttt                                                                                                                                                                    >  1:1252519/1‑233 (MQ=255)
                                                             cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTttttt                                                                                                                                                                >  1:617657/1‑236 (MQ=255)
                                                             cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCTGTTTGTttttt                                                                                                                                                                >  1:918755/1‑236 (MQ=255)
                                                             cTTCCACCTTTTAAAAACGGCCCGTAGGCTTCGCTCCAGCTTTGGGTGACCGTGACCTTTTTCTTCGCCAGTTTCTGCCAGGCTTGGGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTc                                                                                                                                                              <  2:1847094/239‑1 (MQ=255)
                                                                            aacggcccGTGGGCTTCGCCCAAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCATTTTCTGCCGGGCTTGTGGGGCGTCATCGCCATAGCCTTTTTGCATCAATACCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGAt                                                                                                                                                                                                                     <  2:1452761/163‑1 (MQ=255)
                                                                             agtggCCGTAGCCTCCCCCCCAGCTTTGGGTGACCGTGCCCTTTTTCTTCGCCGGTTTCGGCCCGGCTTGGGGGGCGTCTTCGCCATAGCCTTTTTGAATCAATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGTTCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAg                                                                                                                                              <  2:586981/236‑1 (MQ=255)
                                                                                 gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGa                                                                                                                                                                                                                      >  2:134422/1‑163 (MQ=255)
                                                                                 gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGa                                                                                                                                                                                                                      <  1:134422/163‑1 (MQ=255)
                                                                                  ccGTATGTTTCGCCCCAGCTTTGGGTGCCCGTGCCCTTTTTCTTCGCCGTTTTCTGCCAGGTTTGGGGGGCGTCAGCCCCATAGACTTTTTGCCCCAATAGCAACAGCCCCAGCCCGGGTGTACGGGTGCGCGGTTCCTGAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGc                                                                                                                                          <  2:617657/238‑1 (MQ=255)
                                                                                       aGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAAc                                                                                                                                                                                                           >  1:1452761/1‑169 (MQ=255)
                                                                                         gCTTCGCTCCACCCTTTGGTGACGGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTGGTGGGGCGTCATCGCCATAGCCTTTTTGCACCCATAGCAACAGACCCAGCCCCGGTGTACTGGTCCGCGGACCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  1:1559441/170‑1 (MQ=255)
                                                                                            tCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa                                                                                                                                <  1:1176802/239‑1 (MQ=255)
                                                                                            cggcccccgCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGTTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa                                                                                                                                <  1:1358251/231‑1 (MQ=255)
                                                                                            ccGCTCCAGCTTTTGGTGACCGTACCCGTTTCCTTGCCCATTTCCGGCCAGCCTTTGGGGGGTTACGCCCCATGGCTTTTTGGCACCCAAAGCAACAGCCCCAGCCCCGGGGTACTGGTGCCCGGTTCCTGATAAAGCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa                                                                                                                                <  2:1811846/238‑1 (MQ=255)
                                                                                             cGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc                                                                                                                                                                                     >  2:634438/1‑185 (MQ=255)
                                                                                             cGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc                                                                                                                                                                                     <  1:634438/185‑1 (MQ=255)
                                                                                                   aGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGGGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAgg                                                                                                                                                                                              >  2:1559441/1‑170 (MQ=255)
                                                                                                        ttgggaaccgtgactgtTTTTTACGCCAGTTTCTGCCGGGCTGGGGGGGCGCCATCGCCATATCTTTTTTCCACCCATAGCACCGGCCCCATCCCCGTTTTGCTGGGGGCGGGTCCTGAAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTCCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgt                                                                                                                    <  2:1306344/233‑1 (MQ=255)
                                                                                                          ggTGACCGTGACTTTTTTCTTCGCCATTTTCGGCCAGGCTTGGGGGGCGTATGCGCCATAGTTTTTTGGCCACCATAGCACCAGCCCCGGCCCCGGGGTCCTGGGGCGGGTATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgtgt                                                                                                                  <  2:1252519/239‑1 (MQ=255)
                                                                                                              cccgtgacagtTTTCTTCGCTAGTTTCGCCCAGGGTTGGGGGGAGTCATCCCCATAGTCTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTTTACTGGTGCGCGGATCCTAAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGaa                                                                                                                                               <  2:730440/205‑1 (MQ=255)
                                                                                                                        ttttCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        >  2:291368/1‑139 (MQ=255)
                                                                                                                        ttttCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  1:291368/139‑1 (MQ=255)
                                                                                                                               cGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGGTTTTTTTTTTTGTAAAAAAAAGCGAAGTAAGCAGAATCAAACAGTACGAAAAAGTCATTTTTTCAACCCCCgggg                                                                                              >  2:769128/1‑237 (MQ=255)
                                                                                                                                           ccAGGCTTGGGGGGGGTCATCGCCATAGACTTTTTGCACCCATAGCAACAGCCCCAGCCCCGGTGTACGGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg                                                                                                                                                                                       <  2:1530163/137‑1 (MQ=255)
                                                                                                                                                 ttGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTGTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAgg                                                                                                                                                   <  1:1738942/167‑1 (MQ=255)
                                                                                                                                                     ggggCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgtttt                                                                                                                                                                             >  1:1530163/1‑137 (MQ=255)
                                                                                                                                                           tCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGtt                                                                                                                                                                                                                          >  1:1360153/1‑86 (MQ=255)
                                                                                                                                                                           ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc                                                                                                                                                                                                 <  1:233208/96‑1 (MQ=255)
                                                                                                                                                                           ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc                                                                                                                                                                                                 >  2:233208/1‑96 (MQ=255)
                                                                                                                                                                                  tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                     <  1:987822/69‑1 (MQ=255)
                                                                                                                                                                                  tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                     >  2:987822/1‑69 (MQ=255)
                                                                                                                                                                                         cAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAAc                                                                                      <  1:294416/189‑1 (MQ=255)
                                                                                                                                                                                          aaCAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTGGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCg                                                                                                                                                 <  1:1796289/129‑1 (MQ=255)
                                                                                                                                                                                           aCAGCCCCAGCCCGGGTGTACTGGTGCGCGGATCCTAAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg                                                                                               <  1:759297/178‑1 (MQ=255)
                                                                                                                                                                                              gACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAgt                                                                                                                    <  1:1047964/154‑1 (MQ=255)
                                                                                                                                                                                                  cggCCCCGGTGTAGTGGTGCGCGGACCCTAAAAAACCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACa                           <  1:427139/237‑1 (MQ=255)
                                                                                                                                                                                                   aGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTCATAAAAGAAAGGGAAGTAGGCATAAACAAAACGTACGAAAAGGTCATTATTTCCGCCCCCCGGGACGTTAACCGGCTCCGCt                                                                            >  2:294416/1‑189 (MQ=255)
                                                                                                                                                                                                           tgtACTGGTGCGCGGATCCTTATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCtt                                                                <  1:977228/193‑1 (MQ=255)
                                                                                                                                                                                                           tatccTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGTTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa                                                                                              <  1:1347568/159‑1 (MQ=255)
                                                                                                                                                                                                             tcttGGTGCGGGGTCCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc                <  1:35933/236‑1 (MQ=255)
                                                                                                                                                                                                             tccTGGTGGGCGGATCCTAATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc                <  1:1309563/237‑1 (MQ=255)
                                                                                                                                                                                                             tACTGGTGCGCGGTTCCTGAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGc                                                                                                                                          <  2:522185/117‑1 (MQ=255)
                                                                                                                                                                                                               cTGGTGCGCGGTTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCa                                                                                                                                                                           <  2:1581895/82‑1 (MQ=255)
                                                                                                                                                                                                               cTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGtttttta                                                                                                                                                                           >  1:1581895/1‑80 (MQ=255)
                                                                                                                                                                                                                    gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        >  2:397709/1‑48 (MQ=255)
                                                                                                                                                                                                                    gcgcGGATCCTGATAAACCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  1:397709/48‑1 (MQ=255)
                                                                                                                                                                                                                     cgcgGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        <  2:376649/47‑1 (MQ=255)
                                                                                                                                                                                                                     cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                        >  1:376649/1‑47 (MQ=255)
                                                                                                                                                                                                                      gcgGACCCTGAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCATTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTACAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGccc           <  1:1313113/235‑1 (MQ=255)
                                                                                                                                                                                                                       cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTCTTTTTTTCTTTTTATTAACGAAGGCGGAGAAGCCCCAATTaaa                                                                                                                                >  1:522185/1‑117 (MQ=255)
                                                                                                                                                                                                                          aTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACg                                                                                                                              <  1:731201/116‑1 (MQ=255)
                                                                                                                                                                                                                           tCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGcaccac  <  1:1000372/239‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  
ATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCAC  >  NC_000913/74794‑75249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: