Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 417,533 | 0 | T | G | 54.0% | 8.0 / 30.7 | 38 | V131G (GTG→GGG) | phoB | response regulator in two‑component regulatory system with PhoR |
Reads supporting (aligned to +/- strand): ref base T (16/1); new base G (20/0); total (37/1) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-05 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAG‑A‑TGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTAT‑GACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGA > NC_000913/417295‑417761 | ctcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAACCACCTCAAGCGCGAGTCGATGACCCGGGATATTCTAGTGGTGCTTTTGACCGCCAGAGGGTAAGAAGAAGATCGCGTGCGCGGCCTTGAAATCGGCGCGGATGACTATACCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGAGGTAATGCGCCGTATTTTGCCAATGGCGGTGGGAGACGt > 2:1416603/1‑239 (MQ=255) ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGGGGGGATTGAGATGCAggg > 2:123066/1‑239 (MQ=255) cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGAGATGCAGGGATTaa > 1:1162066/1‑239 (MQ=255) cTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGTTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGAt > 1:1158456/1‑239 (MQ=255) aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCGGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGTCGAGGCGGTTTCGAATCAGGGATTAAGTTTTCAACCGCCAACTCACCCGGGGAAggc‑‑gggaggg > 2:132763/1‑235 (MQ=255) tggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGa > 1:320331/1‑200 (MQ=255) gATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCAGCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGAAGAGCCG‑CTGGGG‑A‑GGGGGGCGGCA‑GAAt > 1:1687889/1‑239 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAAAAGCG‑CCGGGG‑A‑TGGGGGCGgaa‑gaaat > 2:1827689/1‑237 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACCTCTCACCGGGTGATGGC‑‑GGGGGGAGAGACG‑CCGGGG‑A‑TGGGGGCgccccaaat > 2:578637/1‑231 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGAAGAGCCG‑CTGGGG‑A‑Tgg > 1:1276564/1‑226 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGCCTCGACCCGACATCTCa > 1:1684516/1‑191 (MQ=255) tGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGTCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCCCACCGGGGGCGGGC‑‑GGGCGGAAAGCCG‑CCGGGG‑A‑TGGGGGCCACA‑AAATTTaaa > 1:1710324/1‑239 (MQ=255) cAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGCGAAGAGCCG‑CGGGGG‑A‑GGGGGGCGACA‑GAATTTtaaat > 1:1122204/1‑232 (MQ=255) gggAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGGCGGGATTTAGTCTCGACCCGACCTCTCACCGGGGGATGGCGGGGGGGAAGAGCGG‑GGGGAG‑‑‑GGGGGCCGACG‑GGTTTTAAAatgcgcc > 2:72979/1‑232 (MQ=255) gaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTGACCGAGTGATGGC‑‑GGGGGGAGGAGGG‑CCGGGG‑G‑GGGGGCCGGCC‑GGATTTTAACTGCTGGACtttttttttgagcc > 2:797592/1‑231 (MQ=255) gaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGGTTAAGGCCCGACCCGACATCTCACCGGGTGGTGGCG‑GGGGGAAGAGCCG‑CGGGGG‑A‑GGGGGCGGAAA‑GAATTTAAATTGCGGGCATTTTTTTT‑GAGGc > 2:206532/1‑239 (MQ=255) cgTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGC‑‑GGGGGGGGAGCCGCCGGGCG‑A‑GGGGGCGCACG‑AAATTTAAA‑TGCTGCCCCTTTTTTT‑GTCggctcccc > 2:1248761/1‑232 (MQ=255) ccTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGGG‑A‑TGGGGCCGACA‑GAATTTAAACTGCTGCCCTTTTTTTT‑GACGCATCCTTAGCGCgtgg > 1:568422/1‑238 (MQ=255) ggCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAAAGGCG‑CCGGGG‑G‑AGGGGGCGGCA‑GAATTTTAAATGGCGCACTTTTTTTT‑g > 1:1112215/1‑210 (MQ=255) cgcgGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGCGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGGCATCTCACCGAGTGATGGG‑‑GGGGGGAGAGGCGCCGGGGG‑G‑GGGGGCCGACG‑GAATTATAACTCGTGCCCTTTTTTTTGGGCACACCGTGAGCGTGTGTagcccgccg > 2:465046/1‑230 (MQ=255) cACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCG‑GGGGGAAAGGCCG‑CGGGGG‑A‑GGGGGCGGCCG‑AAATTTAAA‑TGCTGCCCTTTTTTTT‑TACGCAACCTGAGTGCGGGTACAACCCCGAGCACCTG‑TTTAAacac > 2:162452/1‑238 (MQ=255) ccAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAAGTCTCGAACCGACATCTCCCCGAGTGATGGC‑‑GGGGGGAGAGGCG‑CTGGAG‑G‑TGGGGGCGGCA‑GAATTTAAACTGCTGCACTTTTTTTT‑TTAGCATCCTGAGCGCGTGTACAGCCCCGAGCAGCTG‑TTATAACACg > 2:1307064/1‑237 (MQ=255) ccAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGa > 1:1173491/1‑125 (MQ=255) cAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCa > 1:383898/1‑120 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAGAGCCG‑CTGga > 1:1347913/1‑148 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAAAGGCGCCGGGGG‑A‑GGGGGCGGCAA‑AAATTTaaa > 2:994096/1‑171 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTTAGATGCAGGGGTTAAGCCTCGACCCGTCATCTCACCGAGTGATGGC‑‑GGGCGTAGAAACG‑CCGGGGAA‑TGGGGCCAGAG‑GAATTTCAATTGC‑GCACTTTTTTTT‑TACGCATCCTGTGCGCGTGGACAGCCGCGCGCAGCCG‑CTAAATCACCGCTgg > 2:330576/1‑239 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAGAGGCG‑CTGGGG‑A‑AGGGGGCGGCA‑GAAATTAAACCGCTGGACTTTTTTTT‑GACGCATCCCGAGCGGGGGGTCAACCGCGCGCAGCTGTTTAAACCCCgctgg > 2:1844527/1‑235 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAG‑A‑TGGGGCCGACA‑GAATTTAAAATGCTGCACTTTTTTTT‑GACGCATCCTGAGCGCgtgga > 1:178338/1‑206 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAG‑A‑TGGGGCCCACA‑GAATTTTAAATGCTGCCCCTTTTTAT‑GACGCCTCCTGAGCGGGGGTACAGCCCCGGGGAGCTG‑TTAAACCACGGCTgg > 1:806298/1‑239 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGCGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGGGATGCAGGGCATAAGTCTCGACCCGa > 1:327905/1‑111 (MQ=255) gcggtgggCGCGAATCAAAGCGGGAACGCGCCTTATTTCGCCAATGGCGGTGGAAGAGGTGATTGGAATGCAGGGATTAAGTCTCGACCCGACATCTCCCCGAGTGATGGC‑‑GGGCGAAGCGCCG‑CTGGAG‑A‑TGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTAT‑GACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgtg < 2:884096/232‑1 (MQ=255) gTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATg > 2:304420/1‑48 (MQ=255) tATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGGAAAGGCG‑CTGGGG‑A‑Tgg > 2:567341/1‑101 (MQ=255) tATTTCGCCAATGGCGGGGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGGGAAAAGGCG‑CTGGGG‑ATTGGGGCCG‑CA‑GAATTTTAAATGCTGCCATTTTTTTT‑TACGCCACCTGGGCGCGGGTACAGCcgcgg > 2:79167/1‑164 (MQ=255) tGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGG‑‑GGGGGGAGAGGCG‑CCGGGG‑A‑TGGGGGCGAAA‑GAAATTAAAATGCTGCACTTTTTTTT‑GAAGCATACTGAGGGCGGGTACAGAAGCGAGCAGCTG‑TTTAAACAAGACTGGGGGACTAACGTGGATGGGGGAGAACGCACGGGAGGTGTGCACAATTGTCGCACGCGTAAAga > 2:190928/1‑237 (MQ=255) gTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAGAGCCG‑CCGGGG‑A‑TGGGGGCGGCA‑GAATTTAAAATGCTGCCCTTTTTTTT‑TACGCCTCCTGGGCGCGGGTAAAACCCCCGGGAGCTG‑GTTAACCACCGCTGGGGGACTAACGGGTATGTGGAAGAACGCACGGTCGGTGTCCCCATTTGTCGCCTGCCTAAAGCACTGGa > 2:37237/1‑239 (MQ=255) ggTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAG‑A‑TGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTAT‑GACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGaa > 1:982380/1‑184 (MQ=255) gTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGGAGAGCCG‑CCGGAG‑A‑AGGGGCCGGCAGGAATTTAAA‑TCGTGCACTTTTTTTT‑GAGGCATCCCGAGCGCGTGTACAGCCGCGAGCCGCTG‑TTAAACCACGCCTGGGGGACTAACGTGTTtggtgtaga > 2:1722252/1‑178 (MQ=255) tGATTGAGATGCAGGGGTTAAGt < 2:327905/23‑1 (MQ=255) | CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGC‑‑GGGCGAAGAGCCG‑CTGGAG‑A‑TGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTAT‑GACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGA > NC_000913/417295‑417761 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |