mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Glucose_ALE_7_FLASK_88_Isolate_1_S4_L001_R1_001.good.fq | 1,861,574 | 326,424,128 | 100.0% | 175.3 bases | 239 bases | 99.3% |
| errors | Glucose_ALE_7_FLASK_88_Isolate_1_S4_L001_R2_001.good.fq | 1,861,574 | 326,404,129 | 100.0% | 175.3 bases | 239 bases | 96.0% |
| total | 3,723,148 | 652,828,257 | 100.0% | 175.3 bases | 239 bases | 97.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 139.6 | 6.9 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 17915 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 58 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.006 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.77077 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 14:02:26 22 May 2019 | 14:03:13 22 May 2019 | 47 seconds |
| Read alignment to reference genome | 14:03:13 22 May 2019 | 14:14:08 22 May 2019 | 10 minutes 55 seconds |
| Preprocessing alignments for candidate junction identification | 14:14:08 22 May 2019 | 14:14:59 22 May 2019 | 51 seconds |
| Preliminary analysis of coverage distribution | 14:14:59 22 May 2019 | 14:17:54 22 May 2019 | 2 minutes 55 seconds |
| Identifying junction candidates | 14:17:54 22 May 2019 | 14:18:39 22 May 2019 | 45 seconds |
| Re-alignment to junction candidates | 14:18:39 22 May 2019 | 14:21:43 22 May 2019 | 3 minutes 4 seconds |
| Resolving best read alignments | 14:21:43 22 May 2019 | 14:23:13 22 May 2019 | 1 minute 30 seconds |
| Creating BAM files | 14:23:13 22 May 2019 | 14:25:48 22 May 2019 | 2 minutes 35 seconds |
| Tabulating error counts | 14:25:48 22 May 2019 | 14:26:43 22 May 2019 | 55 seconds |
| Re-calibrating base error rates | 14:26:43 22 May 2019 | 14:26:44 22 May 2019 | 1 second |
| Examining read alignment evidence | 14:26:44 22 May 2019 | 14:36:21 22 May 2019 | 9 minutes 37 seconds |
| Polymorphism statistics | 14:36:21 22 May 2019 | 14:36:21 22 May 2019 | 0 seconds |
| Output | 14:36:22 22 May 2019 | 14:36:39 22 May 2019 | 17 seconds |
| Total | 34 minutes 12 seconds | ||
