Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	127,209	0	T	G	30.9%	112.4 / 11.4	55	G505G (GGT→GGG)	aceF	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base T (9/29);  new base G (17/0);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 4.58e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTG  >  NC_000913/126988‑127445                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              aCCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGGCTGGTTGTTCCGGTAt                                                                                                                                                                                                                               >  1:292223/1‑238 (MQ=255)                ttGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTcaacaa                                                                                                                                                                                                               >  1:354548/1‑239 (MQ=255)                     aGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGaaa                                                                                                                                                                                                           <  2:483081/238‑1 (MQ=255)                        gTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAgg                                                                                                                                                                                                         <  1:577749/237‑1 (MQ=255)                         ttCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGCACGGGCTGGTTGTTCCGGTATTCAAAGAAGTCAACAAGAAAGGca                                                                                                                                                                                                       >  1:400644/1‑238 (MQ=255)                                aaCAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTACCCCGAACGGTCTGGGTGGTTCGGGATTCCAAGACGGCCACAAGAAAAGCATCATCgt                                                                                                                                                                                                >  2:1026434/1‑237 (MQ=255)                                   agcagAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGCTACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGa                                                                                                                                                                                                >  1:747953/1‑235 (MQ=255)                                    gcagAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGGGGGAACCCCCCACGGGCTGGTTTTTCCCGGATTTAAAAATGGCAACAAGAAAAGCATCCTCGAg                                                                                                                                                                                               >  2:29395/1‑235 (MQ=255)                                      agAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTAACCCCAACGGGCTGGTTGTTTCGGGATTTAAAGACGCCAAAAAGAAAGGGATCATCGa                                                                                                                                                                                                >  2:745866/1‑232 (MQ=255)                                      agAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAg                                                                                                                                                                                               <  2:292223/233‑1 (MQ=255)                                      agAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAg                                                                                                                                                                                               <  1:554495/233‑1 (MQ=255)                                      agAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGc                                                                                                                                                                                              <  1:29395/234‑1 (MQ=255)                                             agaagCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTACCCCCAAAGGTCTGGTTTTTCCCGTATTCAAAGACGTCAACAAGAAAGGCATCATCCAGCTGTCTCGCGa                                                                                                                                                                                    >  1:460895/1‑237 (MQ=255)                                                agCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtga                                                                                                                                                                               <  1:745866/239‑1 (MQ=255)                                                agCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtg                                                                                                                                                                                <  1:457461/238‑1 (MQ=255)                                                   ggcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCACGAGCtgatga                                                                                                                                                                            <  1:1005511/239‑1 (MQ=255)                                                   ggcgAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCAAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCtgat                                                                                                                                                                              <  1:1026434/237‑1 (MQ=255)                                                                             tCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATAACCCGAACGGGCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGCGACggg                                                                                                                                                  >  1:508152/1‑238 (MQ=255)                                                                                 cccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCGATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGa                                                                                                                                                                <  2:439243/221‑1 (MQ=255)                                                                                 cccGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGa                                                                                                                                                                >  1:439243/1‑221 (MQ=255)                                                                                           ttCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCCAACGGGCTGGTTGTTCCGGTATTTAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTTTCGCGAGCTGATGGCTATTTCTAAGAAAGCGCGGGACGGTAAGCTGACTGCggg                                                                                                                                    >  1:542687/1‑239 (MQ=255)                                                                                                catGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTAACCCCCACCGGCTGGGTGTTCCGCGATTTAAAGAAGTCAACAAAGAAAGCATCATCGAACTGTCTTGCGAGGTGATGGCTATTTTTTAGAAAACTCGGGACGGTTAGCTGCCTGCTGGCGgaa                                                                                                                               >  2:496849/1‑239 (MQ=255)                                                                                                   gAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGGGGATACCCCCCACGGGCTGGGTGTTCCGGGATTCAAAGACGTCCAAAAGAAAAGCATCATCGAGCCTTCTCCCGAGCTGATGACTATTTTTAAAAAAACCCGGGACGGGAAACTGATCGCGGGGGGAATGc                                                                                                                            >  2:611203/1‑239 (MQ=255)                                                                                                         cGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCa                                                                                                                           <  2:836131/234‑1 (MQ=255)                                                                                                         cGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGACGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCt                                                                                                                                                                                             <  1:1017856/168‑1 (MQ=255)                                                                                                               tgcAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCt                                                                                                                <  1:834578/239‑1 (MQ=255)                                                                                                                   gCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGGACCCCGAACGGGGTGGTTGTTCCGGTATTCAAAGAAGGCAACAAAAAAGGCATCATCGAGCTGTCTCGCGAg                                                                                                                                                                                   >  2:1017856/1‑168 (MQ=255)                                                                                                                   gCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCCAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCa                                                                                                             >  1:282526/1‑238 (MQ=255)                                                                                                                               aTGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATAACCCGAACGGGCTGGTTTTTTCGGGATTCAAAGACGGCAACAAGAAAAGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAACGCGTGGCGGGAAGCTGACTGCGGGCGGAATGCAGGGGGGGTGCTTCACCCACTCCAGCa                                                                                                 >  2:935026/1‑238 (MQ=255)                                                                                                                                   cTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑Tcggc                                                                                           <  2:747953/239‑1 (MQ=255)                                                                                                                                      gCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTACCCCGCACGGGCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAACCCGTGACGGTAAACTGACTGCGGGCGGAATGCAGGGCGGGTGCTTCCCCATCTCCAGCA‑Tcggcgg                                                                                         >  2:308715/1‑238 (MQ=255)                                                                                                                                      gCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCggcggc                                                                                        <  1:294643/239‑1 (MQ=255)                                                                                                                                      gCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACACTGAAGAAATACATCAACATCGGTGTGGCGGTGGATGCCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGGCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGATGATGACGATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGAGTCACCATCTCCAGCA‑TCggcggc                                                                                        <  1:10541/239‑1 (MQ=255)                                                                                                                                        ttCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTcgc                                                                                                                                                                                      <  1:467565/144‑1 (MQ=255)                                                                                                                                                ttCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGGCTGGTTGTTCCGGTATTCAAAAACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGGGGTTGCTTCACCCTCTCCAGCA‑TCGGCGGGCCGGGtact                                                                               >  1:760801/1‑238 (MQ=255)                                                                                                                                                  cGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGTACCCCTAACGGGCTGGTTGTTTCGGGATTTCAAGACGCCAACAAGAAAGGCACCATCGCGCTGGTTCGCGAGTtgatga                                                                                                                                                                            >  2:467565/1‑144 (MQ=255)                                                                                                                                                                            aCCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGGAACCCCCCACGGGCTTGTTGTTCCGGTATTTAAAAAAGTTAACCAAAAAAGCATCCTCGAGGTGTCTTTCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTTACTGTGGGCGGAATTCATGGCGGTTGCTTCACCCTCTCCAGCATTCGCCGCCTTGGGTCTTCCCCACTT‑GCTCCCATTGTTCACGCGcc                                                  >  2:635552/1‑239 (MQ=255)                                                                                                                                                                               cTGAAGAAATACATCAACAGCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGa                                               <  1:512366/239‑1 (MQ=255)                                                                                                                                                                                tGAAGAAATACATCAACATCGGTGTGGCGGTGGGTACCCCGAACGGGCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAAAAAGCGCGTGACGGTAAGCTGACTGCGGGCg                                                                                                                                  >  2:255402/1‑156 (MQ=255)                                                                                                                                                                                tGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCg                                                                                                                                  <  1:255402/156‑1 (MQ=255)                                                                                                                                                                                     aaaTACATCAACAGCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGGTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGc                                         <  2:508152/239‑1 (MQ=255)                                                                                                                                                                                             cAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGAGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTGCCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCg                                 <  2:354548/239‑1 (MQ=255)                                                                                                                                                                                               aCATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGc                               <  1:308715/239‑1 (MQ=255)                                                                                                                                                                                               aCATCGGTGTGGCGGTGGAAACCCCGAACGGGCTGGTTGTTCCGGTATTCAAAGACGTGAACAAGAAAAGCATCATCGAGCCGTCTCGCGAGCCGATGACTATTTTTAAGAAAGCGCGTGACGGTAAGCGGACTGCGGGGGGAATGCAGGGGGGGTGCTTCCCCCTCTCCACCA‑TCGGCGGCCGCGGGACTAACCACCTCGCGCCGCTTGTGACAACGCCCGGAGTggggatactccgc                               >  2:1005057/1‑228 (MQ=255)                                                                                                                                                                                                          gCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCgg                                                                                                                     <  1:838093/143‑1 (MQ=255)                                                                                                                                                                                                          gCGGTGGATACCCCGAACGGGCTGGTTGTTTCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCgg                                                                                                                     >  2:838093/1‑143 (MQ=255)                                                                                                                                                                                                           cGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTc                    <  2:542687/238‑1 (MQ=255)                                                                                                                                                                                                                gATACCCCGAACGGTCTGGTTGTTCCGGGATTTTAAGACGTCAAAAAGAAAGGCCTCATCGAGCTGTCTCCCGAGCTGATGAGTATTTTTAAGAAAGCGGGTGACGGGAAGCTGACTGCGGGCGAAATGCCGGGGGGGTGGTTTTACATCTCCCGCA‑TCGGGAGGCCGGGGACCACCCACCTCCCGCCTCTTGTTGACGCGCCCGACGCGGCGATCCTCCGCGGTTGCCAATCCGCGag              >  2:471528/1‑238 (MQ=255)                                                                                                                                                                                                                    ccccGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAg          <  1:935026/239‑1 (MQ=255)                                                                                                                                                                                                                     cccGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGc         <  2:282526/239‑1 (MQ=255)                                                                                                                                                                                                                     cccGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGc         <  1:750073/239‑1 (MQ=255)                                                                                                                                                                                                                        gAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGCAGTGGCTATCCCCGGCGTTTCCAAATCCCCGATTGGGCCgg      >  2:630400/1‑239 (MQ=255)                                                                                                                                                                                                                          aCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGgtg    <  1:630400/239‑1 (MQ=255)                                                                                                                                                                                                                          aCGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCCGGGAACTACCCCCTTCGCGCCGATTGTGAACGCGCCGGCAGTGGCCATCCCCGGCGTTTCCCAGTCCGCGATGGGGcc        >  2:619319/1‑235 (MQ=255)                                                                                                                                                                                                                            ggTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCAATTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATAGAGCCGGtgtg  <  1:995369/239‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              ACCGATATCACCGAGTTGGAAGCGTTCCGTAAACAGCAGAACGAAGAAGCGGCGAAACGTAAGCTGGATGTGAAGATCACCCCGGTTGTCTTCATCATGAAAGCCGTTGCTGCAGCTCTTGAGCAGATGCCTCGCTTCAATAGTTCGCTGTCGGAAGACGGTCAGCGTCTGACCCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGGTCTGGTTGTTCCGGTATTCAAAGACGTCAACAAGAAAGGCATCATCGAGCTGTCTCGCGAGCTGATGACTATTTCTAAGAAAGCGCGTGACGGTAAGCTGACTGCGGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCA‑TCGGCGGCCTGGGTACTACCCACTTCGCGCCGATTGTGAACGCGCCGGAAGTGGCTATCCTCGGCGTTTCCAAGTCCGCGATGGAGCCGGTGTG  >  NC_000913/126988‑127445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑