| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 647,430 | C→T | R27H (CGC→CAC) | citG ← | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 647,430 | 0 | C | T | 88.6% | 98.5 / 4.6 | 36 | R27H (CGC→CAC) | citG | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (15/16); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
CTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAGGCGGCAGTGAATAGTCGCGGCGGGAGAGAATTTCGCCTTCGG > NC_000913/647212‑647653 | cTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGt > 3:354696/1‑237 (MQ=255) cGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCa < 3:473823/239‑1 (MQ=255) tACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCGTACTCa < 1:185494/230‑1 (MQ=255) ttGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGccagcccagc < 4:220013/212‑1 (MQ=255) ttGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGccagcccagc > 3:220013/1‑212 (MQ=255) ttGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGccag > 3:278661/1‑206 (MQ=255) gCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCACCCGGGAGAACGCATCGATTAATg > 2:185494/1‑230 (MQ=255) tAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCt > 3:450012/1‑239 (MQ=255) cGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTg < 3:158476/239‑1 (MQ=255) tCCGCACTCCAGGCACAAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGa < 4:363920/239‑1 (MQ=255) cGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACa < 3:245888/239‑1 (MQ=255) aCTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCg > 3:356360/1‑239 (MQ=255) cACCAAATTCAATGAACCGGGGTACCCAGCCCGGAATCGCCAGCGCGCTGCGGTGGAAATCCTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTcag < 4:356360/236‑1 (MQ=255) aTGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTTTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGGGCGTCCAttc > 4:246095/1‑237 (MQ=255) tGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCg < 3:246095/239‑1 (MQ=255) aaCGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCa < 3:281179/176‑1 (MQ=255) aaCGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCa > 4:281179/1‑176 (MQ=255) gCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCAcgc < 2:64985/239‑1 (MQ=255) tgCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATg > 3:357027/1‑103 (MQ=255) tgCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATg < 4:357027/103‑1 (MQ=255) cgcACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAg > 1:200355/1‑238 (MQ=255) aCCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACa > 2:187957/1‑143 (MQ=255) aCCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACa < 1:187957/143‑1 (MQ=255) aCCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGtt < 4:80903/169‑1 (MQ=255) aCCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGtt > 3:80903/1‑169 (MQ=255) gCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGAt > 4:213168/1‑177 (MQ=255) gCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGAt < 3:213168/177‑1 (MQ=255) aTCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAgg > 3:284970/1‑232 (MQ=255) aTCCAAGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAgg < 4:284970/232‑1 (MQ=255) cAGATTGACTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAGGCGGCAGTGAATAGTCGCGGCGGGAGAGAAtt > 3:431380/1‑239 (MQ=255) aCTTCAGTCAGCATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAGGCGGCAGTGAATAGTCGCGGCGGGAGAGAATTTCGCCtt < 4:431380/239‑1 (MQ=255) tcaggcagCAGTGCGGGCCAGCCCAGACGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAGGCGGCAGTGAATAGTCGCGGCGGGAGAGAATTTCGCCTTCgg < 4:450012/239‑1 (MQ=255) cATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGt < 4:199489/136‑1 (MQ=255) cATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGt > 3:199489/1‑136 (MQ=255) cATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGa < 4:301554/153‑1 (MQ=255) cATGGCGTGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGa > 3:301554/1‑153 (MQ=255) gcgtgcCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAgg > 3:456551/1‑186 (MQ=255) gcgtgcCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGATTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAgg < 4:456551/186‑1 (MQ=255) | CTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCGTACTCATCGATTAATGACGTCGCAAGCTTTGTGGTTTTAGTTGACGTTGCAGGCATCGACATCGTTCAGCAGTGCCTCCATGCGGTTGAGTAAATCGGTCAGTTGATGGGTTTTTCCACGCGCGCAGACGGCTGCGCTTTGTTCGCACAACAGGCAGCGGCGAGGCGGCAGTGAATAGTCGCGGCGGGAGAGAATTTCGCCTTCGG > NC_000913/647212‑647653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |