Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1540T.21.4% 611.9 / 172.0 225intergenic (+19/+24)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (92/84);  new base . (21/27);  total (113/112)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

CGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGG  >  NC_000913/120056‑120252
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cGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCt                                                                                                     <  1:2156089/99‑1 (MQ=255)
   tttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTa                                                                                                  <  1:3149088/99‑1 (MQ=255)
   tttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTa                                                                                                  >  1:4473411/1‑99 (MQ=255)
       aaTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑ggg                                                                                             >  1:4935087/1‑99 (MQ=255)
         tGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTg                                                                                           >  1:4299809/1‑99 (MQ=255)
           gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAg                                                                                         >  1:5032170/1‑99 (MQ=255)
           gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAg                                                                                         >  1:873839/1‑99 (MQ=255)
           gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAg                                                                                         <  1:1374659/99‑1 (MQ=255)
            ggaagaaCACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  2:4212023/98‑1 (MQ=255)
            cGAAGAAAGCCTCGTTGGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  2:3564498/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAgg                                                                                        <  1:1225736/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAgg                                                                                        <  2:4544623/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAgg                                                                                        <  2:2590073/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAgg                                                                                        <  2:1909803/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  1:3667353/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  1:4767841/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  2:1751340/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  1:2041697/99‑1 (MQ=255)
            cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg                                                                                        <  2:738856/99‑1 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCCCCCTGTAC‑GGGTGAggg                                                                                       >  2:4328165/1‑99 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTCC‑GGGAGAggg                                                                                       >  2:3309879/1‑99 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAggg                                                                                       <  1:954432/99‑1 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAggg                                                                                       <  1:4134874/99‑1 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAggg                                                                                       <  2:829983/99‑1 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAggg                                                                                       <  1:735179/99‑1 (MQ=255)
             gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAggg                                                                                       <  1:2639824/99‑1 (MQ=255)
                     ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTagaga                                                                               >  2:1827373/1‑99 (MQ=255)
                     ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTagaga                                                                               >  1:2202542/1‑99 (MQ=255)
                      cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTagagag                                                                              <  1:4509100/99‑1 (MQ=255)
                         gttcgtGGTCGTGGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAACGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGAtt                                                                           >  2:1222249/1‑99 (MQ=255)
                         gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGAtt                                                                           >  2:3981544/1‑99 (MQ=255)
                                gtcgttgtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATgcgc                                                                    <  2:2826119/99‑1 (MQ=255)
                                  cgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCtt                                                                  <  1:4391452/99‑1 (MQ=255)
                                      gtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc                                                              <  1:3274667/99‑1 (MQ=255)
                                       tggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACg                                                             <  2:4450897/99‑1 (MQ=255)
                                       tggtgATTGCACTACCGACGATTTACCGGGCGTTGGTGTAAAGATTATTGCCGTCCGCCTGTAA‑GGGTGAGGGCGTAGAGAGATTAATGAGCTTTTACg                                                             <  2:451827/99‑1 (MQ=255)
                                        ggggATTGCACTACGGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACgg                                                            <  2:1293744/96‑1 (MQ=255)
                                         gtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGc                                                           >  1:389295/1‑99 (MQ=255)
                                              tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTgg                                                      >  1:2235314/1‑99 (MQ=255)
                                               gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                     <  2:4157285/99‑1 (MQ=255)
                                                cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                    >  1:2780068/1‑99 (MQ=255)
                                                cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                    <  2:3402860/99‑1 (MQ=255)
                                                cACTACTGACGAATTACGTGACGGTGGTGAAAAGATTCTTGCCCTCACCCTGTCC‑GGGGGAGGGCCTAGAGCGATTACTGCGCTTTTACGGCTATGGCg                                                    >  2:4787648/1‑99 (MQ=255)
                                                  ctactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGt                                                  >  1:21300/1‑99 (MQ=255)
                                                   tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GTGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                 >  2:4250611/1‑99 (MQ=255)
                                                    actGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                <  1:4418414/99‑1 (MQ=255)
                                                    actGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                >  1:3864150/1‑99 (MQ=255)
                                                     ctGACGATTTACGGGGCGTTGGTGGAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTGTAGGGCTCTGGCGGtttt                                               <  2:4916731/99‑1 (MQ=255)
                                                      tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGGTTtc                                              >  2:4093889/1‑99 (MQ=255)
                                                       gACGGTGTACGGGGGGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                             <  2:1473710/99‑1 (MQ=255)
                                                        aCGATTTACGGGGCGTTGGTGTATAGATCATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTGTACGGCTTTGGCGGTTTtctc                                            >  1:4837565/1‑99 (MQ=255)
                                                        aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                            >  2:219299/1‑99 (MQ=255)
                                                        aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                            >  1:3506784/1‑99 (MQ=255)
                                                         cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                           <  2:2773662/99‑1 (MQ=255)
                                                         cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                           <  1:2726956/99‑1 (MQ=255)
                                                          gATTTACGGGTCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCtt                                          <  2:2522707/99‑1 (MQ=255)
                                                          gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCtt                                          >  1:3635516/1‑99 (MQ=255)
                                                          gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCCCtt                                          >  2:4551914/1‑99 (MQ=255)
                                                           aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt                                         <  1:2863575/99‑1 (MQ=255)
                                                               aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                     <  1:1980919/99‑1 (MQ=255)
                                                               aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGGGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                     <  2:3245690/99‑1 (MQ=255)
                                                                     cGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAg                               <  1:2555080/99‑1 (MQ=255)
                                                                       ttGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGcc                             <  1:2388439/99‑1 (MQ=255)
                                                                         ggTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                           <  1:1052550/99‑1 (MQ=255)
                                                                            gtAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                        <  1:935914/99‑1 (MQ=255)
                                                                             tAAAGATTATTGCCCTGACCCTGTAC‑GGGTGAGGGCCTAGAGAGATTAATGCGCTTTTACGGCATTGGCGGTTTTCTCTTTAGACAGATAGCCGATAcc                       >  2:304937/1‑99 (MQ=255)
                                                                              aaaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                      <  2:3538056/99‑1 (MQ=255)
                                                                              aaaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                      >  1:894390/1‑99 (MQ=255)
                                                                               aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                     >  1:1748121/1‑99 (MQ=255)
                                                                               aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                     >  2:4037742/1‑99 (MQ=255)
                                                                               aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                     >  1:2249264/1‑99 (MQ=255)
                                                                               aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                     >  1:4322800/1‑99 (MQ=255)
                                                                                aGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                    <  1:1596617/99‑1 (MQ=255)
                                                                                 gATTATTGCCCTCACCCCGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAac                   <  1:3993810/99‑1 (MQ=255)
                                                                                  attattGCCCTCACCTTGTAC‑GGGTGGGGGCGTAGAGCGATGAGAGCGACTTGACGGGTGTGGCGGTTTTCTCCGTAAACAGTGTGCCGATTCCTGacg                  <  2:1447008/99‑2 (MQ=255)
                                                                                     tacgCCCTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGa               >  2:1407421/4‑99 (MQ=38)
                                                                                        gCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa            <  1:806422/99‑1 (MQ=255)
                                                                                        gCCCTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACCCGATCa            >  1:1150283/1‑99 (MQ=39)
                                                                                         cccTCACTCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg           >  2:4870094/1‑99 (MQ=255)
                                                                                         cccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg           >  2:4717074/1‑99 (MQ=255)
                                                                                         cccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg           <  1:4049692/99‑1 (MQ=255)
                                                                                         cccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg           >  1:3560010/1‑99 (MQ=255)
                                                                                         cccTCACCC‑GTACAGGGGGAGGGCGTAGAGCGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg           >  2:2423959/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTGAACAGATAGCCGATACCTTACACGATCAGc          >  2:1073254/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          <  2:228655/99‑1 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:393024/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  2:3620767/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:2237192/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTGGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACCCGATCAGc          >  2:2012421/1‑99 (MQ=255)
                                                                                          ccTCACCCTGTAC‑GGGTGAGGGCGTACAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  2:2993008/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGagcagc          >  1:4651924/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:4280636/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:4224180/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:1721410/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  1:2466536/1‑99 (MQ=255)
                                                                                          ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc          >  2:790027/1‑99 (MQ=255)
                                                                                           cTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc         <  2:3503460/99‑1 (MQ=255)
                                                                                              aCCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATa      >  1:3429965/1‑99 (MQ=255)
                                                                                                 cTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCg   >  1:609292/1‑99 (MQ=255)
                                                                                                  tGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCgg  >  1:2932109/1‑99 (MQ=255)
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CGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGG  >  NC_000913/120056‑120252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑