Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1700CT23.6% 471.4 / 140.5 249intergenic (+54/+30)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (98/90);  new base T (25/33);  total (125/124)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

GATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGA  >  NC_000913/710072‑710267
                                                                                                  |                                                                                                 
gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                                                                                                   >  1:3289823/1‑99 (MQ=255)
gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                                                                                                   >  1:3188275/1‑99 (MQ=255)
 aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCGCTTGCATAAAAAAGCCAACCCg                                                                                                  >  2:510033/1‑99 (MQ=255)
     tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                                                                                              >  1:2199358/1‑99 (MQ=255)
       aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                            >  1:1120738/1‑99 (MQ=255)
        tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTg                                                                                           >  1:4683801/1‑99 (MQ=255)
         aaaGTCTGGCAGGAAATACGCCGTACTAACAAATAAGCGAGAGCTGCAACTCTCGCTTTTCTTATCTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                          <  1:4825581/99‑1 (MQ=255)
           cgTCTAGCAGGAACTACGCAGTAATAACAAATAAGTGAGAGCTGTGACTCTCTCTTTTCTTATTTCCCTTGCATAAAAAAGCCAATCTGCGGGTTGGCt                                                                                        <  2:4735975/98‑1 (MQ=255)
            gTCTGGCAGGAAATACGCAGTAATAACAACTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                       <  2:4339346/99‑1 (MQ=255)
            gTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtt                                                                                       <  1:533815/99‑1 (MQ=255)
             tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttt                                                                                      <  1:1958619/99‑1 (MQ=255)
              gtGGCAGGAGATACGAAGAAATAACGAATAAGTGGGAGCTGTAACTCTCGCGTTTCTTGTTTCCCTTGCATAAAAAAGCCAAACTGCGGGTTGGCtttt                                                                                     <  2:3957286/98‑1 (MQ=255)
              cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttt                                                                                     <  1:4505180/99‑1 (MQ=255)
              cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                     <  1:2523829/99‑1 (MQ=255)
               tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                    <  1:2151597/99‑2 (MQ=255)
               tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                    <  1:1723279/99‑2 (MQ=255)
               tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                    >  2:1890065/1‑99 (MQ=255)
               tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                    <  1:2068723/99‑1 (MQ=255)
                ggCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttttt                                                                                   <  1:5136332/99‑3 (MQ=39)
                 gCAGGAAATACGCAGTAATAACAATTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAACAAAGCCAACCTGCGGGTTGGCtttttta                                                                                  <  2:2198060/99‑4 (MQ=38)
                  cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttat                                                                                 <  1:1478038/99‑5 (MQ=38)
                  cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttat                                                                                 <  2:348996/99‑5 (MQ=38)
                  cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTCGGCttttttat                                                                                 <  1:4461514/99‑5 (MQ=38)
                  cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCg                                                                                 <  2:3867272/99‑1 (MQ=255)
                   aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                >  2:887832/1‑99 (MQ=255)
                       aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAg                                                                            <  1:3507458/99‑1 (MQ=255)
                       aaTACGCAGTAATAACAAATAAGTGAGAGCTGCAACTCTCGCTTTTCTTATTTCCCTTGCATGAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAg                                                                            >  1:4888770/1‑99 (MQ=255)
                        aTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgg                                                                           <  2:3542365/99‑1 (MQ=255)
                           cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                        >  1:2965923/1‑99 (MQ=255)
                           cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                        <  1:232266/99‑1 (MQ=255)
                            gCAGTAATAACAAATAAGTGAGAGCTGTAGCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctgg                                                                       <  1:5073818/99‑1 (MQ=255)
                            gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctgg                                                                       <  2:26145/99‑1 (MQ=255)
                            gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctgg                                                                       >  2:1123578/1‑99 (MQ=255)
                                  ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAtt                                                                 >  2:1214954/1‑99 (MQ=255)
                                  ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAtt                                                                 <  2:4976107/99‑1 (MQ=255)
                                   taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                <  1:471741/99‑1 (MQ=255)
                                   taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                <  1:4453527/99‑1 (MQ=255)
                                   taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAttt                                                                <  1:1093853/99‑1 (MQ=255)
                                     aCAAATAAGTGAGAGTTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                              <  2:3340901/99‑1 (MQ=255)
                                     aCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                              <  1:3346653/99‑1 (MQ=255)
                                     aCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCAGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                              <  1:2584431/99‑1 (MQ=255)
                                      cAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGcc                                                             <  2:2977787/99‑1 (MQ=255)
                                      cAAATAAGTGAGAGCGGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGcc                                                             >  2:1039662/1‑99 (MQ=255)
                                             gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGCCTGGCTTATTTGCCTTCGTgc                                                      >  2:3518534/1‑99 (MQ=255)
                                               gagaGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                    <  2:2435461/99‑1 (MQ=255)
                                               gagaGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                    >  2:3228652/1‑99 (MQ=255)
                                                agagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                   <  2:2153686/99‑1 (MQ=255)
                                                agagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                   >  2:4866206/1‑99 (MQ=255)
                                                 gagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCAt                                                  <  2:2380592/99‑1 (MQ=255)
                                                   gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                >  2:3750688/1‑99 (MQ=255)
                                                    cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                               >  1:2252078/1‑99 (MQ=255)
                                                     tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                              >  2:3730273/1‑99 (MQ=255)
                                                     tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                              >  1:242906/1‑99 (MQ=255)
                                                      gTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                             <  1:709730/99‑1 (MQ=255)
                                                        aaCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                           >  2:391179/1‑99 (MQ=255)
                                                        aaCTCTCGCTTTTCTTATTACCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGCTCAGGGTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                           <  1:4606387/99‑1 (MQ=255)
                                                             tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                      >  1:1779190/1‑99 (MQ=255)
                                                              cGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                     <  2:1988356/99‑1 (MQ=255)
                                                              cGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                     <  2:2920076/99‑1 (MQ=255)
                                                               gCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                    <  2:4370667/99‑1 (MQ=255)
                                                                cTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGGTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGc                                   >  1:304039/1‑99 (MQ=255)
                                                                 ttttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCa                                  >  2:2123976/1‑99 (MQ=255)
                                                                 ttttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCa                                  >  2:846192/1‑99 (MQ=255)
                                                                  tttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                 <  2:944018/99‑1 (MQ=255)
                                                                      ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTTCGCATGTTCATTTTCGCGGCAATCGc                             >  1:63345/1‑99 (MQ=255)
                                                                      ttATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                             >  2:2640712/1‑99 (MQ=255)
                                                                         tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                          <  2:3872536/99‑1 (MQ=255)
                                                                         tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                          >  1:3638373/1‑99 (MQ=255)
                                                                         tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                         <  1:1007028/99‑1 (MQ=255)
                                                                          ttCCCTTGCATAAAAAAGCCCACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                         <  1:3577829/99‑1 (MQ=255)
                                                                            cccGTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCGCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCgg                       <  2:46826/99‑1 (MQ=255)
                                                                             ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGc                      >  1:3530503/1‑99 (MQ=255)
                                                                              cTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                     <  1:1671753/99‑1 (MQ=255)
                                                                               ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcac                    >  2:4585886/1‑99 (MQ=255)
                                                                                tGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                   <  1:712735/99‑1 (MQ=255)
                                                                                tGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                   >  1:904292/1‑99 (MQ=255)
                                                                                 gCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                  >  2:4004181/1‑99 (MQ=255)
                                                                                  cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                 >  2:3782256/1‑99 (MQ=255)
                                                                                   cgagaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTACGCATGTTCATCGTCGCGTCGATCGCCTTCGGCACAGTg                >  1:4855083/5‑99 (MQ=37)
                                                                                   aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                >  2:2651916/1‑99 (MQ=255)
                                                                                     aaaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCACCTTCGGCACAGTGAc              >  2:2019638/1‑99 (MQ=255)
                                                                                      gaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             >  1:3240767/2‑99 (MQ=255)
                                                                                      gaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             >  1:4102939/2‑99 (MQ=255)
                                                                                      gaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             >  2:2721253/2‑99 (MQ=255)
                                                                                      gaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             >  1:4712968/2‑99 (MQ=255)
                                                                                      aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             <  1:2848626/99‑1 (MQ=255)
                                                                                      aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc             <  2:4061591/99‑1 (MQ=255)
                                                                                       aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg            >  1:1318109/1‑99 (MQ=255)
                                                                                         aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  1:1896360/1‑99 (MQ=255)
                                                                                         aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  2:366806/1‑99 (MQ=255)
                                                                                         aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  2:4165429/1‑99 (MQ=255)
                                                                                         aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  2:2907683/1‑99 (MQ=255)
                                                                                         aaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  1:3077034/1‑99 (MQ=255)
                                                                                         aaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa          >  1:423456/1‑99 (MQ=255)
                                                                                           gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa        >  1:2090434/1‑99 (MQ=255)
                                                                                           gCCAACCCGCAGGTTGGCTTTTCTCGTTCGGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa        <  2:4636126/99‑1 (MQ=255)
                                                                                            ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg       <  1:5013989/99‑1 (MQ=255)
                                                                                             cAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa      >  1:1624192/1‑99 (MQ=255)
                                                                                              aaCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt     <  1:4008422/99‑1 (MQ=255)
                                                                                                cccGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag   <  1:864113/99‑1 (MQ=255)
                                                                                                  |                                                                                                 
GATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGA  >  NC_000913/710072‑710267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: